Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,953,902 T→C Q377R (CAG→CGG)  b1872 ← biotin sulfoxide reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,953,9020TC99.6% 754.3 / ‑4.9 234Q377R (CAG→CGG) b1872biotin sulfoxide reductase 2
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (122/111);  total (122/112)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 243 total aligned reads displayed.

ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991
                                                                                          |                                                                                         
aTTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGc                                                                                         >  2:2739383/1‑93 (MQ=255)
 tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGcc                                                                                        >  1:6540681/1‑93 (MQ=255)
 tttCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAAACGCCGCACGGTGTACCAATCCGcc                                                                                        >  2:1251487/1‑93 (MQ=255)
  ttCCGGCAGCACGCCGCCAACACTCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGccc                                                                                       >  1:5831108/1‑93 (MQ=255)
   tCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCt                                                                                      <  2:1883298/93‑1 (MQ=255)
     cGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTaa                                                                                    >  1:6624499/1‑93 (MQ=255)
      ggCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAAc                                                                                   <  1:9357420/93‑1 (MQ=255)
       gcagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACa                                                                                  <  1:3211926/93‑1 (MQ=255)
        cagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAt                                                                                 <  1:2958731/93‑1 (MQ=255)
        cagcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACAt                                                                                 <  2:1356772/93‑1 (MQ=255)
         agcaCGCCGCCAACACGCGTCGGGTTGTCACCGTTGGAGTAATGATAAGAGAAACGAAAACCGCCGCCCGGTGTACAAATCCGCCCTAACATc                                                                                >  1:8739387/1‑93 (MQ=255)
         agcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATc                                                                                <  1:3741586/93‑1 (MQ=255)
          gcaCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcg                                                                               >  2:7242014/1‑93 (MQ=255)
           caCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgc                                                                              <  1:7163531/93‑1 (MQ=255)
           caCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgc                                                                              <  1:5343890/93‑1 (MQ=255)
            aCGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgcc                                                                             >  2:3083090/1‑93 (MQ=255)
             cgccgcCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATcgccg                                                                            >  2:7801928/1‑93 (MQ=255)
               ccgccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCgccgcc                                                                          <  2:642493/93‑1 (MQ=255)
                 gccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAg                                                                        <  1:6844016/93‑1 (MQ=255)
                  ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  2:9902684/93‑1 (MQ=255)
                  ccAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGc                                                                       <  2:239426/93‑1 (MQ=255)
                   cAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCg                                                                      <  1:7456200/93‑1 (MQ=255)
                    aaCACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGt                                                                     >  2:2859980/1‑93 (MQ=255)
                     acacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTc                                                                    <  2:789809/93‑1 (MQ=255)
                      cacGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCa                                                                   <  2:3014771/93‑1 (MQ=255)
                       acGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCAc                                                                  >  1:9168186/1‑93 (MQ=255)
                          cgTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAg                                                               >  1:3479883/1‑93 (MQ=255)
                           gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              >  1:5107310/1‑93 (MQ=255)
                           gTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGc                                                              <  1:609255/93‑1 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  1:4998614/1‑93 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  1:2233251/1‑93 (MQ=255)
                            tCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCa                                                             >  2:5175145/1‑93 (MQ=255)
                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           <  2:4338714/93‑1 (MQ=255)
                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           <  1:9791916/93‑1 (MQ=255)
                              gggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATc                                                           >  2:4024863/1‑93 (MQ=255)
                               ggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATcc                                                          >  2:804024/1‑93 (MQ=255)
                               ggTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATcc                                                          <  2:7091554/93‑1 (MQ=255)
                                gTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCa                                                         <  1:7738393/93‑1 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        >  1:248774/1‑93 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        <  2:7411349/93‑1 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        >  1:9214049/1‑93 (MQ=255)
                                 ttGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAg                                                        <  1:1636084/93‑1 (MQ=255)
                                  tGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgt                                                       <  2:2508764/93‑1 (MQ=255)
                                   gCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAgtg                                                      <  2:378405/93‑1 (MQ=255)
                                     cACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGtt                                                    <  2:8584234/93‑1 (MQ=255)
                                      cccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   <  2:6148732/92‑1 (MQ=255)
                                      aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   >  2:2002410/1‑93 (MQ=255)
                                      aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   <  2:4926849/93‑1 (MQ=255)
                                      aCCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGttt                                                   <  1:5263031/93‑1 (MQ=255)
                                       ccGTTGGGGTAATGATAAGAGAAACCAAAACCCCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCCTCACCTGCCTCCAGTGTTTc                                                  >  2:6852567/1‑93 (MQ=255)
                                       ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  2:8343650/93‑1 (MQ=255)
                                       ccGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTc                                                  <  1:7853598/93‑1 (MQ=255)
                                        cGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCt                                                 >  2:9733400/1‑93 (MQ=255)
                                         gTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTg                                                >  1:8134348/1‑93 (MQ=255)
                                            ggAGCAATCATAAGAGAAACCAAAACCGCCCCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTc                                             <  1:3102471/93‑1 (MQ=255)
                                               gTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCAcc                                          <  1:4232097/93‑1 (MQ=255)
                                                tAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCa                                         >  2:2680618/1‑93 (MQ=255)
                                                 aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        >  2:146753/1‑93 (MQ=255)
                                                 aaTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCat                                        >  1:2374206/1‑93 (MQ=255)
                                                  aTGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCata                                       >  2:2291219/1‑93 (MQ=255)
                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:1582776/1‑93 (MQ=255)
                                                   tGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCatat                                      >  2:3453910/1‑93 (MQ=255)
                                                    gaaaaGAGAAACCAAAACCGCCGCCCGGTGTACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATAtt                                     <  1:2144216/90‑1 (MQ=255)
                                                      tAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGc                                   <  2:1172773/93‑1 (MQ=255)
                                                       aaGAGAAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCt                                  >  1:3991359/1‑93 (MQ=255)
                                                          agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               <  2:5726195/93‑1 (MQ=255)
                                                          agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               >  1:8850118/1‑93 (MQ=255)
                                                          agaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgc                               <  1:9445686/93‑1 (MQ=255)
                                                           gaAACCAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGgcg                              <  2:6802217/93‑1 (MQ=255)
                                                              aCCAAAACCGCCCCCCGGTCTACCAATCCGCCCTAACAGCGCCGCCAGCCTCACCAGCATCCAGTGTTTCTGTTCCCCATATTGCTGGCGCTg                           <  2:2051610/93‑1 (MQ=255)
                                                               ccAAAACCGCCGCCCGTTGTGCCAATCCGCCCTAACACCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa                          <  1:964550/93‑1 (MQ=255)
                                                               ccAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa                          >  1:1983449/1‑93 (MQ=255)
                                                               ccAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGa                          <  1:8326441/93‑1 (MQ=255)
                                                                cAAACCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  2:1377793/93‑1 (MQ=255)
                                                                cAAAACCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGaa                         <  2:8859699/93‑1 (MQ=255)
                                                                 aaaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt                        <  2:4549849/93‑1 (MQ=255)
                                                                 aaaaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAAt                        <  1:1174728/93‑1 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      <  1:9066615/93‑1 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      >  2:3430184/1‑93 (MQ=255)
                                                                   aaCCGCCGCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTc                      >  1:2260104/1‑93 (MQ=255)
                                                                     ccgccgCCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTccc                    >  2:6703538/1‑93 (MQ=255)
                                                                      cgccgcCCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTcccc                   <  2:2566898/93‑1 (MQ=255)
                                                                        ccgccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAg                 <  1:3308076/93‑1 (MQ=255)
                                                                          gccCGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGcc               <  2:5814098/93‑1 (MQ=255)
                                                                           cccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCt              >  1:4561428/1‑93 (MQ=255)
                                                                            ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             >  2:6954722/1‑93 (MQ=255)
                                                                            ccGGTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTg             >  1:4609785/1‑93 (MQ=255)
                                                                              ggTGTACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGcc           >  1:2628835/1‑93 (MQ=255)
                                                                                tgtACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         <  1:1236292/93‑1 (MQ=255)
                                                                                tgtACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCAt         >  2:10042744/1‑93 (MQ=255)
                                                                                 gtACCAACCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCGGCCATc        <  2:5575518/93‑1 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  1:4708688/93‑1 (MQ=255)
                                                                                  tACCAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCa       <  2:4077149/93‑1 (MQ=255)
                                                                                    ccAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGc     <  1:7550879/93‑1 (MQ=255)
                                                                                     cAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa    <  1:5669534/93‑1 (MQ=255)
                                                                                     cAATCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCa    >  1:4333310/1‑93 (MQ=255)
                                                                                      aaTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAt   >  2:9104770/1‑93 (MQ=255)
                                                                                       aTCCGCCCTTACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  >  2:62780/1‑93 (MQ=255)
                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  <  1:683105/93‑1 (MQ=255)
                                                                                       aTCCGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCAtt  >  2:4452678/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATTTCCGGCAGCACGCCGCCAACACGCGTCGGGTTGCCACCGTTGGAGTAATGATAAGAGAAACCAAAACCGCCGCCCGGTGTACCAATCTGCCCTAACATCGCCGCCAGCGTCACCAGCATCCAGTGTTTCTGTTCACCATATTGCTGGCGCTGAATTCCCCAGCCTGCCATCAGCATT  >  NC_000913/1953812‑1953991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑