BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,957,451	T→C	L140L (CTA→CTG)	b1875 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,957,451	0	T	C	99.6%	846.1 / NA	263	L140L (CTA→CTG)	b1875	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (120/142); minor base A (1/0); total (121/142)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

AGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCC  >  NC_000913/1957361‑1957539

aGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAg                                                                                        <  1:23312/93‑1 (MQ=255)

 gttgttTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAgg                                                                                       <  1:7180271/93‑1 (MQ=255)

   tgttTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCt                                                                                     <  2:6757003/93‑1 (MQ=255)

    gttTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTg                                                                                    >  1:8314085/1‑93 (MQ=255)

     ttttGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGa                                                                                   <  2:214161/93‑1 (MQ=255)

      tttGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAg                                                                                  >  2:3630887/1‑93 (MQ=255)

       ttGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGt                                                                                 <  2:4917069/93‑1 (MQ=255)

        tGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTc                                                                                >  1:3810404/1‑93 (MQ=255)

          ccATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCt                                                                              <  1:8918445/93‑1 (MQ=255)

          ccATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCt                                                                              <  2:6312136/93‑1 (MQ=255)

          ccATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCt                                                                              >  2:894205/1‑93 (MQ=255)

           cATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCtt                                                                             <  1:4090202/93‑1 (MQ=255)

           cATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCtt                                                                             >  2:9716348/1‑93 (MQ=255)

            aTCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTtc                                                                            <  2:1382166/93‑1 (MQ=255)

            aTCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTtc                                                                            >  1:6390439/1‑93 (MQ=255)

             tCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTtca                                                                           >  1:6086662/1‑93 (MQ=255)

              cGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTtcat                                                                          <  1:4745466/93‑1 (MQ=255)

                   aCCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGaa                                                                     <  1:1006264/93‑1 (MQ=255)

                    ccGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGaaa                                                                    <  2:3032745/93‑1 (MQ=255)

                    ccGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGaaa                                                                    <  2:8513287/93‑1 (MQ=255)

                     cGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAg                                                                   >  2:8103883/1‑93 (MQ=255)

                      gCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGCAAGc                                                                  >  2:6276381/1‑93 (MQ=255)

                      gCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGc                                                                  >  1:3681532/1‑93 (MQ=255)

                      gCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGc                                                                  >  1:7576841/1‑93 (MQ=255)

                       ccAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCa                                                                 <  2:9624732/93‑1 (MQ=255)

                        cAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCaa                                                                <  1:9915886/93‑1 (MQ=255)

                        cAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCaa                                                                <  2:3024701/93‑1 (MQ=255)

                        cAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCaa                                                                <  1:7496310/93‑1 (MQ=255)

                        cAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCaa                                                                >  1:7905084/1‑93 (MQ=255)

                        cAGCGTCGGATTCGGT‑ATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAAt                                                               <  1:6705720/93‑1 (MQ=255)

                         aGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAAt                                                               <  2:3270828/93‑1 (MQ=255)

                         aGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAAt                                                               <  1:4485465/93‑1 (MQ=255)

                         aGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAAt                                                               >  1:7863605/1‑93 (MQ=255)

                          gCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATg                                                              >  2:2820069/1‑93 (MQ=255)

                            gTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGcc                                                            <  1:5664481/93‑1 (MQ=255)

                            gTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGCCCTTCATCAGAAAGCAATGcc                                                            <  1:1777716/93‑1 (MQ=255)

                             tCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCa                                                           <  2:6564108/93‑1 (MQ=255)

                              cGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAg                                                          <  1:1259543/93‑1 (MQ=255)

                              cGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAg                                                          <  1:4152711/93‑1 (MQ=255)

                               ggATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAgc                                                         <  2:2081932/93‑1 (MQ=255)

                               cgATTCGGTAAGCGCTCATGCTCGCCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAgc                                                         <  2:3583506/92‑1 (MQ=255)

                                gATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAgcg                                                        >  1:3300009/1‑93 (MQ=255)

                                gATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAgcg                                                        >  1:4796148/1‑93 (MQ=255)

                                 aTTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAgcgc                                                       <  2:4090857/93‑1 (MQ=255)

                                 aTTCGGTAATCGCTCATGCTCACCTTTTGGCGAACCGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAgcgc                                                       >  1:8980423/1‑93 (MQ=255)

                                   tCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCAc                                                     <  1:3091286/93‑1 (MQ=255)

                                   tCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCAc                                                     <  2:7216070/93‑1 (MQ=255)

                                    cGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACg                                                    >  2:1652897/1‑93 (MQ=255)

                                     ggTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACgg                                                   >  2:7846082/1‑93 (MQ=255)

                                     ggTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACgg                                                   >  2:744602/1‑93 (MQ=255)

                                       tAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGc                                                 >  1:4304596/1‑93 (MQ=255)

                                       tAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGc                                                 >  2:5623369/1‑93 (MQ=255)

                                        aaTCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCa                                                >  1:6837606/1‑93 (MQ=255)

                                         aTCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCAt                                               <  2:211058/93‑1 (MQ=255)

                                          tCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCAtt                                              >  1:3953415/1‑93 (MQ=255)

                                             cTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAg                                           >  2:4245628/1‑93 (MQ=255)

                                             cTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAg                                           >  1:1519464/1‑93 (MQ=255)

                                             cTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAg                                           >  1:6142665/1‑93 (MQ=255)

                                              tCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGc                                          <  1:265684/93‑1 (MQ=255)

                                                 tGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGtt                                       >  2:4492085/1‑93 (MQ=255)

                                                 tGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGtt                                       >  1:7818547/1‑93 (MQ=255)

                                                  gCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGttt                                      >  2:5975370/1‑93 (MQ=255)

                                                  gCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGttt                                      >  1:627920/1‑93 (MQ=255)

                                                      aCCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTgg                                  <  2:2411578/93‑1 (MQ=255)

                                                        ctgttGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGAt                                <  1:9284165/90‑1 (MQ=255)

                                                        cTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGAt                                >  2:1898319/1‑93 (MQ=255)

                                                        cTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGAt                                <  1:8499208/93‑1 (MQ=255)

                                                            tGGCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCAcc                            <  2:8604125/93‑1 (MQ=255)

                                                             ggCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCAccc                           <  2:3955660/93‑1 (MQ=255)

                                                             ggCGAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCAccc                           <  1:2537853/93‑1 (MQ=255)

                                                                gAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGc                        <  2:3535934/93‑1 (MQ=255)

                                                                gAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGc                        <  1:5877184/93‑1 (MQ=255)

                                                                gAACTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGc                        >  2:4969188/1‑93 (MQ=255)

                                                                 aaCTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCa                       >  2:6455548/1‑93 (MQ=255)

                                                                 aaCTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCa                       >  2:5383/1‑93 (MQ=255)

                                                                 aaCTGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCa                       <  1:9168126/93‑1 (MQ=255)

                                                                    tGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGaa                    <  2:4291335/93‑1 (MQ=255)

                                                                    tGGTTTTCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATAACCCGGCAGaa                    >  1:2782521/1‑93 (MQ=255)

                                                                       ttttCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGa                 <  2:7629366/93‑1 (MQ=255)

                                                                        tttCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGAt                <  2:9006909/93‑1 (MQ=255)

                                                                        tttCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGAt                <  2:9380157/93‑1 (MQ=255)

                                                                         ttCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGAtt               >  1:442660/1‑93 (MQ=255)

                                                                          tCACTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGAttt              <  2:9809562/93‑1 (MQ=255)

                                                                           cccTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTc             <  2:1087844/91‑1 (MQ=255)

                                                                            aCTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCGGGCAGAACGATTTCa            >  1:5489958/1‑93 (MQ=255)

                                                                             cTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCaa           >  2:3988609/1‑93 (MQ=255)

                                                                             cTGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCaa           >  1:1178689/1‑93 (MQ=255)

                                                                             cTGAGAACCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCaa           <  1:8745274/93‑1 (MQ=255)

                                                                              tGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCATCGTTTCTGGATCACCCGGCAGAACGATTTCAat          >  1:9701978/1‑93 (MQ=255)

                                                                              tGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAat          >  1:710062/1‑93 (MQ=255)

                                                                              tGAGATCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAat          >  2:1728429/1‑93 (MQ=255)

                                                                               gagaTCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAata         >  1:5204283/1‑93 (MQ=255)

                                                                                agaTCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAatat        <  2:5587899/93‑1 (MQ=255)

                                                                                agaTCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAatat        <  2:8643586/93‑1 (MQ=255)

                                                                                   tCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGtt     >  2:8277166/1‑93 (MQ=255)

                                                                                   tCCCCGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGtt     >  1:7038062/1‑93 (MQ=255)

                                                                                    ccccGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTc    <  1:3141711/93‑1 (MQ=255)

                                                                                      ccGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTccc  <  1:939757/93‑1 (MQ=255)

                                                                                      ccGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTccc  >  1:2010459/1‑93 (MQ=255)

                                                                                      ccGGCAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTccc  <  1:10006200/93‑1 (MQ=255)

AGTTGTTTTGCCATCGGTGACCGCCAGCGTCGGATTCGGTAATCGCTCATGCTCACCTTTTGGCGAACTGGTTTTCACTGAGATCCCCGGTAGGCTGAGTCCTTCATCAGAAAGCAATGCCAGCGCACGGGCATTCAGCGTTTCTGGATCACCCGGCAGAACGATTTCAATATGTTCCC  >  NC_000913/1957361‑1957539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`