BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,972,288	G→A	T354M (ACG→ATG)	cheA ←	CheA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,972,288	0	G	A	98.8%	856.1 / ‑2.8	241	T354M (ACG→ATG)	cheA	CheA
Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (109/128); total (110/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 255 total aligned reads displayed.

TGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGA  >  NC_000913/1972198‑1972377

tGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATc                                                                                         >  2:5014940/1‑93 (MQ=255)

tGATCGAGGCTATTGCGTACCAGATGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATc                                                                                         <  2:6891030/93‑1 (MQ=255)

 gATCGATGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCa                                                                                        <  2:4021747/93‑1 (MQ=255)

 gATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCa                                                                                        >  1:8416797/1‑93 (MQ=255)

  gtCGAGGGTATTTCGTACCAGGTTGGTCAGCGGGTTCATAATGCGTTCTATCAGGCTTTTGTCGGGTTCAGTAGAACTGCCCACCAGCATCAg                                                                                       <  1:2182692/92‑1 (MQ=255)

   tCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGt                                                                                      >  2:4735768/1‑93 (MQ=255)

   tCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGt                                                                                      <  1:5135492/93‑1 (MQ=255)

    cGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGtt                                                                                     >  1:2657285/1‑93 (MQ=255)

     gaagCTACTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCATTTc                                                                                    <  1:6637995/90‑1 (MQ=255)

     gAGGCTATTGCGTCCCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTc                                                                                    <  2:2426634/93‑1 (MQ=255)

     gAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTc                                                                                    <  2:9931249/93‑1 (MQ=255)

       ggCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTa                                                                                  >  2:10041525/1‑93 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 <  2:5958490/93‑1 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 >  2:3450133/1‑93 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 <  2:7695539/93‑1 (MQ=255)

        gCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTAc                                                                                 >  1:6029347/1‑93 (MQ=255)

          tATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCt                                                                               <  1:6319471/93‑1 (MQ=255)

           aTTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTg                                                                              >  1:3586931/1‑93 (MQ=255)

           aTTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTg                                                                              >  1:3958230/1‑93 (MQ=255)

             tGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCt                                                                            <  2:3407670/93‑1 (MQ=255)

                 tACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGcc                                                                        >  1:9604952/1‑93 (MQ=255)

                   ccAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGa                                                                      >  1:6998340/1‑93 (MQ=255)

                    cAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAg                                                                     >  1:6895956/1‑93 (MQ=255)

                      ggTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGtt                                                                   >  1:860649/1‑93 (MQ=255)

                      ggTGGGTAAGCGGGTCGATGATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGtt                                                                   <  1:4141809/93‑1 (MQ=255)

                       gTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGttt                                                                  <  2:6240583/93‑1 (MQ=255)

                         gggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTc                                                                >  2:6605281/1‑93 (MQ=255)

                         gggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTc                                                                >  2:857729/1‑93 (MQ=255)

                          ggTGAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACGGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTcc                                                               <  2:4244948/93‑1 (MQ=255)

                          ggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTcc                                                               >  2:165686/1‑93 (MQ=255)

                          ggTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTcc                                                               <  1:4163814/93‑1 (MQ=255)

                           gTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTccc                                                              <  1:1142778/93‑1 (MQ=255)

                           gTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTccc                                                              <  1:7855038/93‑1 (MQ=255)

                           gTCAGCGGGTCGATAATGCGTGCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTccc                                                              <  1:1941769/93‑1 (MQ=255)

                            tCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCg                                                             >  2:8258227/1‑93 (MQ=255)

                             cAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGc                                                            >  1:6608123/1‑93 (MQ=255)

                               gCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCa                                                          >  1:3742775/1‑93 (MQ=255)

                                 gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                        <  2:5805144/93‑1 (MQ=255)

                                 gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                        >  1:2531873/1‑93 (MQ=255)

                                 gggTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGa                                                        >  2:7189505/1‑93 (MQ=255)

                                  ggTCGATAATGCGTTCTATCAGGCTGTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTGTTCCCGCCAGAt                                                       <  2:6011987/93‑1 (MQ=255)

                                   gttgATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                      >  1:23322/4‑93 (MQ=255)

                                   gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTCGCAATGCCCACCAGCCTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGAta                                                      <  2:8990372/93‑2 (MQ=255)

                                   gTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATc                                                      <  1:2952508/93‑1 (MQ=255)

                                    tCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCa                                                     <  1:660222/93‑1 (MQ=255)

                                    tCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCa                                                     >  2:5220195/1‑93 (MQ=255)

                                      gATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACg                                                   >  1:5058978/1‑93 (MQ=255)

                                        tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGcc                                                 >  1:4649624/1‑92 (MQ=255)

                                        tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                                 >  1:886203/1‑93 (MQ=255)

                                        tAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCa                                                 <  2:8499480/93‑1 (MQ=255)

                                             cGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGc                                            <  2:4552665/93‑1 (MQ=255)

                                              gTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCCCGCACCAGcc                                           >  2:9794531/1‑93 (MQ=255)

                                               ttCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCg                                          >  2:4880978/1‑93 (MQ=255)

                                                 cTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCGGCATCAGTTGTACCTGCTTGCCGAGGTTTCGCGCCAGATCACGAAACAGGCggg                                        >  1:1102320/1‑93 (MQ=255)

                                                 cTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCggg                                        <  2:7308765/93‑1 (MQ=255)

                                                 cTATCAGGCTTTGGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCggg                                        <  1:7458897/93‑1 (MQ=255)

                                                  tATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCgggg                                       <  1:5547062/93‑1 (MQ=255)

                                                   aTCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                      <  2:452543/93‑1 (MQ=255)

                                                   aTCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                      <  2:7369314/93‑1 (MQ=255)

                                                   aTCAGGCTTTTGGCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGa                                      <  1:7094862/93‑1 (MQ=255)

                                                    acAGGCTTTTCTCGAGTTGAGTAGATCTGCCCAGCAGCATCAGTTCTGCCTGCTTGCCGAGTTTTCCCGCCAGATCACGCGCCAGCCGGGGAt                                     <  2:6756919/92‑1 (MQ=255)

                                                      aGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAg                                   >  1:9045272/1‑93 (MQ=255)

                                                      aGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGCGATAg                                   >  1:8786228/1‑93 (MQ=255)

                                                         cTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGa                                <  2:7266883/93‑1 (MQ=255)

                                                         cTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGa                                >  1:8287860/1‑93 (MQ=255)

                                                          ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAc                               <  2:9715048/93‑1 (MQ=255)

                                                          ttttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGAc                               <  2:2090553/93‑1 (MQ=255)

                                                           tttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACt                              <  1:9144185/93‑1 (MQ=255)

                                                            ttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTa                             >  1:3246276/1‑93 (MQ=255)

                                                            ttGTCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTa                             >  1:8029127/1‑93 (MQ=255)

                                                               tCGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTaaaa                          <  1:4499813/93‑1 (MQ=255)

                                                                cGAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTaaaaa                         >  2:2729510/1‑93 (MQ=255)

                                                                 gAGTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAAc                        <  2:2995213/93‑1 (MQ=255)

                                                                   gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                      <  2:2510668/93‑1 (MQ=255)

                                                                   gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                      >  2:8733266/1‑93 (MQ=255)

                                                                   gTTCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACat                      <  1:7608261/93‑1 (MQ=255)

                                                                    ttCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACata                     <  2:5490057/93‑1 (MQ=255)

                                                                    ttCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACata                     <  1:2602844/93‑1 (MQ=255)

                                                                    ttCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACata                     <  1:7220063/93‑1 (MQ=255)

                                                                     tCAGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACatat                    <  1:8148641/93‑1 (MQ=255)

                                                                       cgTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTc                  <  1:97137/92‑1 (MQ=255)

                                                                       aGTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTc                  <  1:1414691/93‑1 (MQ=255)

                                                                        gTAGAACTGCCCCCCCGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTcc                 <  2:7717111/93‑1 (MQ=255)

                                                                        gTAGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTcc                 >  2:642355/1‑93 (MQ=255)

                                                                          aGAACTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCGCCAGCCGGGGATAGCGACTAAAAACATATTCCAt               <  2:9395775/93‑1 (MQ=255)

                                                                            aaCTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCg             >  2:6294166/1‑93 (MQ=255)

                                                                              cTGCCCACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGc           >  1:5505106/1‑93 (MQ=255)

                                                                                 cccACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc        <  1:3479075/93‑1 (MQ=255)

                                                                                 cccACCAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc        <  2:1778942/93‑1 (MQ=255)

                                                                                 cccACAAGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatc        <  1:4329066/93‑1 (MQ=255)

                                                                                  ccaccaGGATCTGTAATACCTGCTTGGCGAGTTTTCCCGCCAGATCGCGCTCCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatca       <  2:7818216/93‑1 (MQ=255)

                                                                                  ccaccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatca       >  2:3029613/1‑93 (MQ=255)

                                                                                  ccaccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatca       <  2:4171087/93‑1 (MQ=255)

                                                                                   caccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGcatcat      <  1:1292777/93‑1 (MQ=255)

                                                                                    accaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg     <  1:1790270/93‑1 (MQ=255)

                                                                                    accaGCATCAGTTCTACCTGCTTGCCGAGTTTTACCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATg     >  2:7503024/1‑93 (MQ=255)

                                                                                     ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc    <  1:8318975/93‑1 (MQ=255)

                                                                                     ccaGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGc    >  2:1288229/1‑93 (MQ=255)

                                                                                      caGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCg   >  2:7905620/1‑93 (MQ=255)

                                                                                       aGCATCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGa  >  2:3822949/1‑93 (MQ=255)

TGATCGAGGCTATTGCGTACCAGGTGGGTCAGCGGGTCGATAATGCGTTCTATCAGGCTTTTGTCGAGTTCAGTAGAACTGCCCACCAGCGTCAGTTCTACCTGCTTGCCGAGTTTTCCCGCCAGATCACGCACCAGCCGGGGATAGCGACTAAAAACATATTCCATCGGCATCATGCGA  >  NC_000913/1972198‑1972377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`