BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	232,218	A→G	D98G (GAC→GGC)	yafE →	putative biotin synthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	232,218	0	A	G	100.0%	1247.7 / NA	390	D98G (GAC→GGC)	yafE	putative biotin synthesis protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (210/180); total (210/180)

Only 100 of 399 total aligned reads displayed.

GTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCA  >  NC_000913/232128‑232307

 ttATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGt                                                                                        >  2:3945469/1‑93 (MQ=255)

 ttATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGt                                                                                        >  2:5388633/1‑93 (MQ=255)

  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       <  1:5715537/93‑1 (MQ=255)

  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       <  2:265111/93‑1 (MQ=255)

  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       <  2:7972923/93‑1 (MQ=255)

  tATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTa                                                                                       >  1:1979258/1‑93 (MQ=255)

   aTTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTaa                                                                                      <  1:8559770/93‑1 (MQ=255)

     tCTGCCCGTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATg                                                                                    >  1:842937/1‑93 (MQ=255)

     tCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATg                                                                                    >  2:8768863/1‑93 (MQ=255)

     tCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATg                                                                                    <  2:1985560/93‑1 (MQ=255)

        gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtct                                                                                 >  2:2983870/1‑93 (MQ=255)

        gCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtct                                                                                 >  2:9067985/1‑93 (MQ=255)

         cccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtctc                                                                                >  2:6993508/1‑93 (MQ=255)

         cccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtctc                                                                                <  2:6110800/93‑1 (MQ=255)

         cccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtctc                                                                                <  1:8740669/93‑1 (MQ=255)

         cccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGtctc                                                                                <  2:5191507/93‑1 (MQ=255)

          ccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTcc                                                                               >  1:5140316/1‑93 (MQ=255)

          ccATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGGGAATAGGATATTGAAACCTGGAGGTAGGCTGATTGCGGTTGGCGTAATGtcttc                                                                               >  2:979735/1‑91 (MQ=255)

           cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCg                                                                              >  1:1645750/1‑93 (MQ=255)

           cATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCg                                                                              >  2:9380089/1‑93 (MQ=255)

            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             <  2:4898585/93‑1 (MQ=255)

            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             >  1:6421278/1‑93 (MQ=255)

            aTCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCgg                                                                             <  1:7714885/93‑1 (MQ=255)

             tCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCggg                                                                            <  2:7039694/93‑1 (MQ=255)

              cACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGt                                                                           >  2:9167390/1‑93 (MQ=255)

               aCTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGta                                                                          >  2:4721731/1‑92 (MQ=255)

                cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTcc                                                                         >  1:592976/1‑92 (MQ=255)

                cTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCa                                                                         >  2:5936569/1‑93 (MQ=255)

                  ggCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCAcc                                                                       >  1:9142304/1‑93 (MQ=255)

                   gCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCAccc                                                                      <  2:761971/93‑1 (MQ=255)

                     atgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAg                                                                    <  2:5771324/93‑1 (MQ=255)

                      tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGt                                                                   >  1:1061236/1‑93 (MQ=255)

                      tgatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGt                                                                   <  1:8267684/93‑1 (MQ=255)

                       gatgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTg                                                                  <  1:3617522/93‑1 (MQ=255)

                        atgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgc                                                                 >  1:7497661/1‑93 (MQ=255)

                        atgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgc                                                                 >  1:3179041/1‑93 (MQ=255)

                         tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTCATGTCTCCGGGTCACCCAGTgcg                                                                >  2:5535389/1‑93 (MQ=255)

                         tgTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTgcg                                                                >  1:2551393/1‑93 (MQ=255)

                         ggTTGGGGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGGCGCCGGGTCACCCAGTgcg                                                                >  2:8964427/2‑93 (MQ=255)

                           ttGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGcgcg                                                              >  1:9802568/1‑93 (MQ=255)

                            tGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGa                                                             <  1:10012395/93‑1 (MQ=255)

                            tGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGa                                                             <  1:9952218/93‑1 (MQ=255)

                            tGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTCCGCGa                                                             >  2:3354524/1‑93 (MQ=255)

                               tGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACAt                                                          >  2:4558996/1‑93 (MQ=255)

                                gcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATc                                                         >  1:4308555/1‑93 (MQ=255)

                                gcagcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATc                                                         <  1:3861905/93‑1 (MQ=255)

                                   gcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTgg                                                      >  1:1906576/1‑93 (MQ=255)

                                   gcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTgg                                                      >  2:7162306/1‑93 (MQ=255)

                                   gcaCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTgg                                                      <  1:7906709/93‑1 (MQ=255)

                                     aCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                                    >  2:4166306/1‑93 (MQ=255)

                                     aCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                                    >  2:330210/1‑93 (MQ=255)

                                     aCTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGtt                                                    <  1:6701157/93‑1 (MQ=255)

                                       tGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTAc                                                  <  2:9472824/93‑1 (MQ=255)

                                        gCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACa                                                 <  2:4364671/93‑1 (MQ=255)

                                         cGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAg                                                <  1:3360383/93‑1 (MQ=255)

                                          gagaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGa                                               <  1:2267198/93‑1 (MQ=255)

                                           agaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                                              >  2:6486605/1‑93 (MQ=255)

                                           agaAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGAc                                              <  1:1382556/93‑1 (MQ=255)

                                             aaGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACgg                                            >  1:9644476/1‑93 (MQ=255)

                                              attGAATCGGATATTGAAACGTGTGGGTACGCTTATTGTGATGAGCGTAATGCCTCGGGGTCCCCCAGTGCGCGACATCTGGTTACAGACGGt                                           <  2:6295030/91‑1 (MQ=255)

                                              aGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGt                                           <  2:2265466/93‑1 (MQ=255)

                                               gTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTa                                          >  2:4397061/1‑93 (MQ=255)

                                                tGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAg                                         <  1:3967248/93‑1 (MQ=255)

                                                 gAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGa                                        <  2:6243707/93‑1 (MQ=255)

                                                  aaTAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGaa                                       >  2:3564175/1‑93 (MQ=255)

                                                  aaTAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGaa                                       <  1:8981192/93‑1 (MQ=255)

                                                   aTAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAg                                      >  2:1841752/1‑93 (MQ=255)

                                                       gATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAtt                                  <  1:526783/93‑1 (MQ=255)

                                                       gATATTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCAtt                                  <  1:1247444/93‑1 (MQ=255)

                                                        atatTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTa                                 <  2:9659214/93‑1 (MQ=255)

                                                          atTGAAACCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTAcg                               >  1:4155950/1‑93 (MQ=255)

                                                             taaaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGa                            <  1:5474200/92‑1 (MQ=255)

                                                              aaaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGAt                           >  2:6506727/1‑93 (MQ=255)

                                                               aaCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATa                          <  1:9239443/93‑1 (MQ=255)

                                                                aCCTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATAc                         >  1:5024928/1‑93 (MQ=255)

                                                                  cTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct                       <  1:7206808/93‑1 (MQ=255)

                                                                  cTGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACct                       >  2:9276532/1‑93 (MQ=255)

                                                                   tGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctc                      >  1:485883/1‑93 (MQ=255)

                                                                   tGGCGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACctc                      <  1:9526716/93‑1 (MQ=255)

                                                                      cGGTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCa                   <  2:7865126/93‑1 (MQ=255)

                                                                       ggTAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCAc                  <  2:1466802/93‑1 (MQ=255)

                                                                         tAGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGt                <  2:8628511/93‑1 (MQ=255)

                                                                          aGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCGCCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTa               >  2:6468490/1‑93 (MQ=255)

                                                                          aGGCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTa               <  1:2653857/93‑1 (MQ=255)

                                                                           ggCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTAc              <  2:9213368/93‑1 (MQ=255)

                                                                            gCTGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACg             >  2:121637/1‑93 (MQ=255)

                                                                              tGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaa           >  2:6724348/1‑93 (MQ=255)

                                                                              tGATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaa           >  1:2418910/1‑93 (MQ=255)

                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          <  1:7352917/93‑1 (MQ=255)

                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          <  2:8362511/93‑1 (MQ=255)

                                                                               gATTGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGaaa          <  1:295526/93‑1 (MQ=255)

                                                                                 ttGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACt        >  1:697521/1‑93 (MQ=255)

                                                                                 ttGTGATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACt        >  1:4506116/1‑93 (MQ=255)

                                                                                 ttGTGATGGGCCGCATGTGTCCGGGTCTCCGAGGGCGAGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACt        <  1:2616432/93‑1 (MQ=255)

                                                                                  tgtgATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTa       <  2:7380038/93‑1 (MQ=255)

                                                                                    tgATGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACg     >  1:5812508/1‑93 (MQ=255)

                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  >  1:8147241/1‑93 (MQ=255)

                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  >  1:1154998/1‑93 (MQ=255)

                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  <  2:3799764/93‑1 (MQ=255)

                                                                                       tGGGCGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCa  >  2:1346872/1‑93 (MQ=255)

GTTATTCTGCCCATCACTGGCATGATGTTGGTGCAGCACTGCGAGAAGTGAATAGGATATTGAAACCTGGCGGTAGGCTGATTGTGATGGACGTAATGTCTCCGGGTCACCCAGTGCGCGACATCTGGTTACAGACGGTAGAAGCATTACGCGATACCTCTCACGTACGAAACTACGCCA  >  NC_000913/232128‑232307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`