Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,063,343 T→C A148A (GCA→GCG)  cobU ← cobinamide kinase/cobinamide phosphate guanylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,063,3430TC99.6% 886.4 / ‑5.0 277A148A (GCA→GCG) cobUcobinamide kinase/cobinamide phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (137/139);  total (137/140)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

Only 100 of 289 total aligned reads displayed.

TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA  >  NC_000913/2063253‑2063432
                                                                                          |                                                                                         
tACTCCAATCCCCGAAACCCCCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCgcgc                                                                                         <  1:7443185/93‑1 (MQ=255)
  cTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCa                                                                                       <  2:2834781/93‑1 (MQ=255)
    ccAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                     <  1:9099234/93‑1 (MQ=255)
    ccAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAga                                                                                     <  1:1200744/93‑1 (MQ=255)
     cAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgac                                                                                    >  2:10043590/1‑93 (MQ=255)
      aaTCCCCGAAACCACCAGCCATACTTCATTTGCCGCATCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacg                                                                                   <  1:6719219/93‑1 (MQ=255)
       atccccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacga                                                                                  <  2:8097960/90‑1 (MQ=255)
        tccccGAACCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 <  2:1958030/91‑1 (MQ=255)
        tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAgacgac                                                                                 <  1:9217884/93‑1 (MQ=255)
        tACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAgacgac                                                                                 >  1:5949285/1‑93 (MQ=255)
         aCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAct                                                                                <  2:479710/93‑1 (MQ=255)
         aCCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGAct                                                                                <  1:3901695/93‑1 (MQ=255)
          cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               >  1:1691670/1‑93 (MQ=255)
          cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               >  1:2699054/1‑93 (MQ=255)
          cccGAAACCACCAGCCATACTTCATTTGCCGCAGCGCCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActc                                                                               <  2:5625363/93‑1 (MQ=255)
           ccGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGActct                                                                              >  2:3892461/1‑93 (MQ=255)
            cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             <  1:7383572/93‑1 (MQ=255)
            cGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACtctc                                                                             >  2:4710689/1‑93 (MQ=255)
              aacccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           <  1:8046768/90‑1 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           >  2:182190/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           >  2:6212200/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           >  1:7038192/1‑93 (MQ=255)
              aaaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCg                                                                           <  2:4907710/93‑1 (MQ=255)
               aaCCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCgg                                                                          >  2:3735537/1‑93 (MQ=255)
                 ccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGca                                                                        <  1:2741010/93‑1 (MQ=255)
                 ccaccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGca                                                                        >  2:8826538/1‑93 (MQ=255)
                  caccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGCCTCTCCGGcac                                                                       >  1:3306821/1‑93 (MQ=255)
                  caccaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcac                                                                       >  1:1016548/1‑93 (MQ=255)
                   accaGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGcaca                                                                      >  2:5229524/1‑93 (MQ=255)
                     caagcATACTTCATTTGCCGCAGCGGCCAACCTCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGGCTCTCCGGCACAAt                                                                    <  2:3059735/89‑1 (MQ=255)
                     caGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAAt                                                                    >  2:9523724/1‑93 (MQ=255)
                      aGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATc                                                                   <  2:5016981/93‑1 (MQ=255)
                       gCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcc                                                                  >  2:8445883/1‑93 (MQ=255)
                        ccATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATccc                                                                 <  1:7726418/93‑1 (MQ=255)
                         cATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATcccc                                                                >  2:4078682/1‑93 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               >  1:4432618/1‑93 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               >  2:7332939/1‑93 (MQ=255)
                          aTACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCa                                                               >  1:2523681/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:2083027/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              <  2:7172225/93‑1 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  1:940750/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  2:4786533/1‑93 (MQ=255)
                           tACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAt                                                              >  2:4223820/1‑93 (MQ=255)
                            aCTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCAtc                                                             >  1:3649357/1‑92 (MQ=255)
                               tCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTccc                                                          >  2:1302774/1‑93 (MQ=255)
                                cATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCa                                                         >  2:9740831/1‑93 (MQ=255)
                                  tttGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACt                                                       >  1:3268724/1‑93 (MQ=255)
                                   ttGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACtc                                                      >  1:5357185/1‑92 (MQ=255)
                                    tGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTc                                                     <  2:3622241/93‑1 (MQ=255)
                                    tGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTc                                                     <  1:2361919/93‑1 (MQ=255)
                                     gcggCAGCGGCCAACTGCTGATTTGCCCGCCCGGCCATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCg                                                    <  1:5155141/90‑1 (MQ=255)
                                     gCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCg                                                    >  1:4596030/1‑93 (MQ=255)
                                      ccGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGt                                                   <  2:8155902/93‑1 (MQ=255)
                                       cGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCGCAATCCCCATTCCCACTTCGtt                                                  <  2:7752885/93‑1 (MQ=255)
                                          aGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGt                                               >  2:5425948/1‑93 (MQ=255)
                                          aGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGt                                               <  2:1528934/93‑1 (MQ=255)
                                            cGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCa                                             >  1:6597663/1‑93 (MQ=255)
                                              gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           >  1:4247176/1‑93 (MQ=255)
                                              gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           >  1:4356091/1‑93 (MQ=255)
                                              gCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACt                                           <  1:9929733/93‑1 (MQ=255)
                                                 aaCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAAt                                        >  2:1246472/1‑93 (MQ=255)
                                                  aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       >  2:5885841/1‑93 (MQ=255)
                                                  aCTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATa                                       >  2:3929619/1‑93 (MQ=255)
                                                     gctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACaa                                    <  1:8530272/93‑1 (MQ=255)
                                                     gctcATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACaa                                    <  1:888891/93‑1 (MQ=255)
                                                      ctgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAc                                   <  2:1699613/93‑1 (MQ=255)
                                                       tgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                  >  2:6496993/1‑93 (MQ=255)
                                                       tgATTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                  >  1:3226780/1‑93 (MQ=255)
                                                       tgATGTACCCGCCCGGCAATATCACTAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAAcc                                  <  1:9142124/93‑1 (MQ=255)
                                                         aTTTACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCtt                                >  2:9140160/1‑93 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             >  1:182014/1‑93 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             <  1:3575675/93‑1 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             <  2:3362524/93‑1 (MQ=255)
                                                            tACCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGc                             <  1:2006447/93‑1 (MQ=255)
                                                             aCCCGCCCGGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCg                            <  2:7576519/93‑1 (MQ=255)
                                                              cccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCgg                           >  2:3230562/1‑93 (MQ=255)
                                                               ccgcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCACACTTCGTTAGTAACTAATACCACCTTTGCggg                          >  2:6118913/1‑93 (MQ=255)
                                                                 gcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGc                        <  2:6054760/93‑1 (MQ=255)
                                                                 gcccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGc                        <  1:4833066/93‑1 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                       >  1:4532387/1‑93 (MQ=255)
                                                                  cccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCa                       >  1:6380176/1‑93 (MQ=255)
                                                                   ccgGCAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCaa                      <  1:4156344/93‑1 (MQ=255)
                                                                       cAATATCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGtt                  <  1:6529921/93‑1 (MQ=255)
                                                                         atatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTgg                <  1:9581948/93‑1 (MQ=255)
                                                                          tatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggc               >  2:8783489/1‑93 (MQ=255)
                                                                          tatCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggc               >  2:2208483/1‑93 (MQ=255)
                                                                           atCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcg              >  2:8543052/1‑92 (MQ=255)
                                                                           atCACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggca              >  2:2378534/1‑93 (MQ=255)
                                                                             cACGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTggcagg            <  1:10004239/93‑1 (MQ=255)
                                                                               cGAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAGTACAACCTTTGCGGGGCAACGTTggcaggca          <  2:6695716/93‑1 (MQ=255)
                                                                                gAAAATGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGgcaggcag         >  2:5680530/1‑93 (MQ=255)
                                                                                 aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        >  2:2595278/1‑93 (MQ=255)
                                                                                 aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagc        >  1:7145103/1‑93 (MQ=255)
                                                                                 aaaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTAGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgaagc        >  2:8509199/1‑93 (MQ=255)
                                                                                  aaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagca       >  1:5463803/1‑93 (MQ=255)
                                                                                  aaaTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGgcagca       >  1:8675949/1‑93 (MQ=255)
                                                                                    aTGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAAt     <  1:8157223/93‑1 (MQ=255)
                                                                                     tGTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATc    <  1:5973524/93‑1 (MQ=255)
                                                                                      gTCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCa   >  1:5803153/1‑93 (MQ=255)
                                                                                       tCGCGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCaa  >  1:8271779/1‑93 (MQ=255)
                                                                                          |                                                                                         
TACTCCAATACCCGAAACCACCAGCCATACTTCATTTGCCGCAGCGGCCAACTGCTGATTTACCCGCCCGGCAATATCACGAAAATGTCGTGCCAGACGACTCTCCGGCACAATCCCCATTCCCACTTCGTTAGTCACTAATACAACCTTTGCGGGGCAACGTTGGCAGGCAGCAATCAA  >  NC_000913/2063253‑2063432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑