Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,097,074 T→C T188A (ACC→GCC)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,097,0740TC99.8% 1348.7 / ‑5.0 417T188A (ACC→GCC) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (236/180);  total (236/181)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 422 total aligned reads displayed.

CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163
                                                                                          |                                                                                         
cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaa                                                                                         >  1:8015351/1‑93 (MQ=255)
cATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaa                                                                                         >  2:7428949/1‑93 (MQ=255)
 aTAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCaaa                                                                                        <  1:7336112/93‑1 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAAc                                                                                       >  1:5882497/1‑93 (MQ=255)
  tAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGGCGGCAAAc                                                                                       >  1:4857259/1‑93 (MQ=255)
   aGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACa                                                                                      >  2:3095220/1‑93 (MQ=255)
    gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     >  2:2837082/1‑93 (MQ=255)
    gCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAg                                                                                     <  1:2451961/93‑1 (MQ=255)
      tATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   >  1:1297179/1‑93 (MQ=255)
      caTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCa                                                                                   <  2:4655166/92‑1 (MQ=255)
       aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  >  1:2009220/1‑93 (MQ=255)
       aTCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCAt                                                                                  <  1:7765585/93‑1 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 >  1:2756321/1‑93 (MQ=255)
        tCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  1:2622218/93‑1 (MQ=255)
        tCCAGGTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATc                                                                                 <  1:886521/93‑1 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  1:325486/1‑93 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                <  2:8237178/93‑1 (MQ=255)
         ccAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCg                                                                                >  2:4751815/1‑93 (MQ=255)
           aGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGATTAATCGCTTCTGCTTCAGTGGATTCGGCAAACAGCATCggg                                                                              >  1:9431869/1‑93 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  1:3269791/1‑93 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             <  1:1380992/93‑1 (MQ=255)
            gTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGa                                                                             >  1:876622/1‑93 (MQ=255)
             ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            >  2:7933449/1‑93 (MQ=255)
             ttCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGat                                                                            >  2:9642100/1‑93 (MQ=255)
                 ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATAttt                                                                        <  1:8268998/93‑1 (MQ=255)
                 ttAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATAttt                                                                        <  1:7014998/93‑1 (MQ=255)
                     aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    <  1:9981003/93‑1 (MQ=255)
                     aGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCt                                                                    >  1:6495158/1‑93 (MQ=255)
                      gTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCtt                                                                   <  2:8060778/93‑1 (MQ=255)
                       tACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTa                                                                  >  1:6082841/1‑93 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  2:8488995/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  2:3456021/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 <  1:8414599/93‑1 (MQ=255)
                        aCGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTaa                                                                 >  2:1122265/1‑93 (MQ=255)
                          gCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATc                                                               <  1:7209952/93‑1 (MQ=255)
                           ccACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATcg                                                              >  1:9513083/1‑93 (MQ=255)
                              cgcgCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGTCAAACAGCATCGGGATATTTTGCTTAATTgcgc                                                           >  1:9668672/1‑93 (MQ=255)
                              cgcgCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCgcgc                                                           >  2:3006705/1‑93 (MQ=255)
                              cgcgCATCGCCAGGTAGGTGTATACAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCgcgc                                                           <  2:2015412/93‑1 (MQ=255)
                               gcgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGcc                                                          <  1:5350538/93‑1 (MQ=255)
                                cgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCt                                                         <  2:452217/93‑1 (MQ=255)
                                cgcATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCt                                                         >  2:4868705/1‑93 (MQ=255)
                                  cATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTc                                                       >  1:1555031/1‑93 (MQ=255)
                                   aTCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTcc                                                      <  2:7241740/93‑1 (MQ=255)
                                        cAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTaa                                                 >  2:4241653/1‑93 (MQ=255)
                                         aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  2:3576085/1‑93 (MQ=255)
                                         aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  2:5677014/1‑93 (MQ=255)
                                         aggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAAc                                                >  1:4044545/1‑93 (MQ=255)
                                          ggtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACa                                               >  2:3869757/1‑93 (MQ=255)
                                           gtaggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACag                                              >  2:9070673/1‑93 (MQ=255)
                                            taggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACaga                                             >  2:5478484/1‑93 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            >  1:7441859/1‑93 (MQ=255)
                                             aggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACagag                                            <  2:5896828/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  2:489796/93‑1 (MQ=255)
                                              ggtGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagc                                           <  2:751920/93‑1 (MQ=255)
                                               gtgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCACACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGGAACCGagca                                          >  1:3624296/1‑93 (MQ=255)
                                                tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         >  2:1699948/1‑93 (MQ=255)
                                                tgtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcag                                         <  1:1028829/93‑1 (MQ=255)
                                                 gtTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGagcagc                                        >  1:1808171/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGGGCGGGCAACCAGAATCGGGATATTTGGCTTATTCGAGCCTTCCTGTAACAGggcagca                                       >  1:5110726/1‑92 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:2288266/1‑93 (MQ=255)
                                                  tttGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCg                                       >  2:6909253/1‑93 (MQ=255)
                                                    tGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaa                                     >  1:4148242/1‑93 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  2:657098/93‑1 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  2:8894664/93‑1 (MQ=255)
                                                     gCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGaaa                                    <  1:5706087/93‑1 (MQ=255)
                                                      cAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAAc                                   <  2:142679/93‑1 (MQ=255)
                                                        aaaaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGt                                 >  2:9808868/1‑93 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  2:7345754/93‑1 (MQ=255)
                                                          aaGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTc                               <  2:1833234/93‑1 (MQ=255)
                                                           aGTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCt                              >  1:9891203/1‑93 (MQ=255)
                                                            gTTTAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTg                             >  1:3820233/1‑93 (MQ=255)
                                                             tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            <  1:4382341/93‑1 (MQ=255)
                                                             tttAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgc                            <  1:9241005/93‑1 (MQ=255)
                                                               tAATCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTgcgc                          >  2:3665989/1‑93 (MQ=255)
                                                                 aTCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGt                        <  2:6444676/93‑1 (MQ=255)
                                                                 aTCGCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGt                        <  1:6807519/93‑1 (MQ=255)
                                                                    gCTTCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCt                     <  2:2610020/93‑1 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGcgcg                         >  1:1902497/1‑87 (MQ=255)
                                                                      ttCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCATTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGa                   >  2:5323431/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  1:9719660/1‑93 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  <  1:2891430/93‑1 (MQ=255)
                                                                       tCTGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGaa                  >  1:6187180/1‑93 (MQ=255)
                                                                         tGCTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACg                >  2:9510017/1‑93 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              >  2:9266807/1‑93 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              <  2:3210748/93‑1 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              >  1:5452916/1‑93 (MQ=255)
                                                                           cTTCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCt              >  2:5145061/1‑93 (MQ=255)
                                                                            ttCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTc             >  1:6643429/1‑93 (MQ=255)
                                                                             tCAGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCa            >  1:1703391/1‑93 (MQ=255)
                                                                               aGTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCAcc          >  2:7738/1‑93 (MQ=255)
                                                                                gTGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCg         >  1:8613717/1‑93 (MQ=255)
                                                                                 tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  1:4638261/93‑1 (MQ=255)
                                                                                 tGGAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGa        <  2:6150364/93‑1 (MQ=255)
                                                                                  ggAGTCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt       >  2:1299166/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCCCCGATGACa   >  1:9000207/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   >  2:8656896/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACa   >  1:2254566/1‑93 (MQ=255)
                                                                                      tCGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCGCagaa         >  1:2170163/1‑84 (MQ=255)
                                                                                       cGGCAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACaa  <  2:8393132/93‑1 (MQ=255)
                                                                                          |                                                                                         
CATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAA  >  NC_000913/2096984‑2097163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑