Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,111,654 T→C A232A (GCA→GCG)  galF ← putative a UDP‑gal transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,111,6540TC99.7% 974.3 / ‑5.1 304A232A (GCA→GCG) galFputative a UDP‑gal transferase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (139/164);  total (140/164)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 321 total aligned reads displayed.

TCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGC  >  NC_000913/2111565‑2111744
                                                                                         |                                                                                          
tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCa                                                                                         >  2:7474606/1‑93 (MQ=255)
tCGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCa                                                                                         >  1:9232013/1‑93 (MQ=255)
 cGTAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCAc                                                                                        <  2:2760043/93‑1 (MQ=255)
   tAACTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                      >  1:255989/1‑93 (MQ=255)
   caaCTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCa                                                                                      <  2:2899804/92‑1 (MQ=255)
     aCTGTCGCCGGGCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCCGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCGACGCACCCgg                                                                                    >  2:3045621/1‑93 (MQ=255)
      cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                   >  2:7197581/1‑93 (MQ=255)
      cTGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGc                                                                                   >  1:3255907/1‑93 (MQ=255)
       tGTCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCt                                                                                  >  2:3554993/1‑93 (MQ=255)
         tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGTAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                                <  2:2556755/93‑1 (MQ=255)
         tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTTCCCACGCACCAGGCTGa                                                                                >  1:8824481/1‑93 (MQ=255)
         tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                                <  2:9769781/93‑1 (MQ=255)
         tCGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGa                                                                                <  2:4776120/93‑1 (MQ=255)
          cGCCGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAg                                                                               <  1:5620087/93‑1 (MQ=255)
            ccGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTa                                                                             >  1:794980/1‑93 (MQ=255)
             cGGTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTAc                                                                            <  2:6922789/93‑1 (MQ=255)
              ggTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACg                                                                           >  2:9180497/1‑93 (MQ=255)
               gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGGTCGGCAATAGCATCAGTCAGCTTAATACGTCCCGACGCACCAGGCGGAGGACGt                                                                          >  1:2401664/1‑93 (MQ=255)
               gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt                                                                          >  2:6042097/1‑93 (MQ=255)
               gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt                                                                          >  1:4794792/1‑93 (MQ=255)
               gTCATCAGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGt                                                                          >  1:4650499/1‑93 (MQ=255)
                tcatcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTTCCCACGCACCAGGCTGAGTACGtt                                                                         >  2:3845720/1‑93 (MQ=255)
                tcatcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGtt                                                                         >  1:5958948/1‑93 (MQ=255)
                  atcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTcc                                                                       <  2:3417913/93‑1 (MQ=255)
                   tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                      >  2:5937112/1‑93 (MQ=255)
                   tcaGCGTTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCa                                                                      <  1:7485900/93‑1 (MQ=255)
                      gcgttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGtt                                                                   >  1:10038373/4‑93 (MQ=255)
                         ttGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCg                                                                <  2:9672311/93‑1 (MQ=255)
                           gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGc                                                              >  2:9336345/1‑93 (MQ=255)
                           gCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCACCACGCAACAGGCTGAGTACGTTCCAGTTCCCGc                                                              >  1:6131320/1‑93 (MQ=255)
                              tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaa                                                           <  1:4314112/93‑1 (MQ=255)
                              tCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaa                                                           >  1:6099975/1‑93 (MQ=255)
                               cAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCaaa                                                          <  2:1838605/93‑1 (MQ=255)
                                aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCCAat                                                         >  1:4932894/1‑93 (MQ=255)
                                aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                         >  2:6272863/1‑93 (MQ=255)
                                aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                         >  1:7090785/1‑93 (MQ=255)
                                aaCGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAat                                                         >  2:6656238/1‑93 (MQ=255)
                                   ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                      >  1:3063384/1‑93 (MQ=255)
                                   ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                      <  2:9586241/93‑1 (MQ=255)
                                   ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                      <  1:3487255/93‑1 (MQ=255)
                                   ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGGCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                      >  1:1813054/1‑93 (MQ=255)
                                   ggATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGCCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATc                                                      >  2:8391030/1‑93 (MQ=255)
                                    gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                     >  1:9045455/1‑93 (MQ=255)
                                    gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                     <  1:166193/93‑1 (MQ=255)
                                    gATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCg                                                     <  1:2725587/93‑1 (MQ=255)
                                     aTTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCgg                                                    <  1:1524455/93‑1 (MQ=255)
                                     aTTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCgg                                                    >  2:4868031/1‑93 (MQ=255)
                                     aTTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCgg                                                    <  2:8537843/93‑1 (MQ=255)
                                        gTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAg                                                 <  1:4232762/93‑1 (MQ=255)
                                         ttttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGa                                                >  1:8291989/1‑93 (MQ=255)
                                           ttttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGaaa                                              <  1:2872609/93‑1 (MQ=255)
                                            tttCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAg                                             >  1:2642206/1‑93 (MQ=255)
                                               cGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcac                                          <  2:2499087/93‑1 (MQ=255)
                                               cGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcac                                          <  2:5150095/93‑1 (MQ=255)
                                                gCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGcaca                                         <  2:2187880/93‑1 (MQ=255)
                                                 ccAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACAt                                        <  2:4455052/93‑1 (MQ=255)
                                                    gCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                     <  1:6323372/93‑1 (MQ=255)
                                                    gCTCGGCAATAGCAGCAGTCAGCGGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGc                                     <  1:6636564/93‑1 (MQ=255)
                                                     cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCg                                    <  1:6395991/93‑1 (MQ=255)
                                                     cTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCg                                    >  1:8072081/1‑93 (MQ=255)
                                                      taggCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                   >  2:8091730/3‑93 (MQ=255)
                                                      tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                   >  1:630971/1‑93 (MQ=255)
                                                      tCGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGa                                   >  1:9416568/1‑93 (MQ=255)
                                                       cGGCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAc                                  <  1:2604097/93‑1 (MQ=255)
                                                        cgCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGAcc                                 <  1:2945352/92‑1 (MQ=255)
                                                         gCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCt                                >  2:7319442/1‑93 (MQ=255)
                                                         gCAATAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCt                                >  2:7615105/1‑93 (MQ=255)
                                                           aaTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTAc                              >  1:5817675/1‑93 (MQ=255)
                                                            aTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCCAATATCGGCAGAAAGCACATAGCGACCTaag                             >  1:5703440/1‑91 (MQ=255)
                                                            aTAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACg                             >  2:7894329/1‑93 (MQ=255)
                                                             tAGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACgg                            >  2:9085263/1‑93 (MQ=255)
                                                              aGCATCAGTCAGCTGACTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                           <  1:3659850/93‑1 (MQ=255)
                                                              aGCATCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGc                           >  2:1569178/1‑93 (MQ=255)
                                                                 aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                        >  1:1134103/1‑93 (MQ=255)
                                                                 aTCAGTCAGCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCat                        <  1:3877137/93‑1 (MQ=255)
                                                                  tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                       <  1:6493469/93‑1 (MQ=255)
                                                                  tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatg                       >  1:4973605/1‑93 (MQ=255)
                                                                  tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGCCCata                       >  1:1367153/1‑92 (MQ=255)
                                                                  tcagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCCACGGCCatg                       >  2:4193304/1‑93 (MQ=255)
                                                                   cagtcagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCatga                      <  1:9583153/93‑1 (MQ=255)
                                                                       cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                  >  2:1249965/1‑93 (MQ=255)
                                                                       cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                  <  2:879549/93‑1 (MQ=255)
                                                                       cagCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTc                  >  1:4425454/1‑93 (MQ=255)
                                                                        agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt                 <  1:8578843/93‑1 (MQ=255)
                                                                        agCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCt                 <  2:7950845/93‑1 (MQ=255)
                                                                         gCTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTg                >  1:9876749/1‑93 (MQ=255)
                                                                          cTGAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGa               <  2:2571385/93‑1 (MQ=255)
                                                                            gAATACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGt             >  2:5067714/1‑93 (MQ=255)
                                                                             aaTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTc            <  2:7141438/93‑1 (MQ=255)
                                                                             aaTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTc            >  1:5362354/1‑93 (MQ=255)
                                                                             aaTACGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTc            >  2:4064112/1‑93 (MQ=255)
                                                                             aaTACGTCCCCACGCACCAGGCTGAGGACGTTCCAGTTCCGGCCAGATATCGGCAGAAAGGACATAGCGACCTACGGCCATGATGTCTGAGTc            >  2:462618/1‑93 (MQ=255)
                                                                                aCGTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAg         <  1:1999793/93‑1 (MQ=255)
                                                                                  gTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCg       >  1:8181077/1‑93 (MQ=255)
                                                                                  gTCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGAGACCTACGGCCATGATGTCTGAGTCCAGCg       >  1:1276425/1‑93 (MQ=255)
                                                                                   tCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGt      >  1:727361/1‑93 (MQ=255)
                                                                                   tCCCCACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGt      >  2:1787154/1‑93 (MQ=255)
                                                                                    ccccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTc     <  2:3739444/93‑1 (MQ=255)
                                                                                     cccACGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCt    <  2:5752519/93‑1 (MQ=255)
                                                                                       cATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGc  >  1:7751159/1‑93 (MQ=255)
                                                                                         |                                                                                          
TCGTAACTGTCGCCGGTCATCAGCATTGCATCAACGGATTGTTTTTTCGCCAGCTCGGCAATAGCATCAGTCAGCTGAATACGTCCCCATGCACCAGGCTGAGTACGTTCCAGTTCCGGCCAAATATCGGCAGAAAGCACATAGCGACCTACGGCCATGATGTCTGAGTCCAGCGTCTGC  >  NC_000913/2111565‑2111744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑