BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,115,180	T→C	Q416R (CAG→CGG)	wcaK ←	putative galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,115,180	0	T	C	100.0%	711.8 / NA	223	Q416R (CAG→CGG)	wcaK	putative galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (114/109); total (114/109)

Only 100 of 230 total aligned reads displayed.

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

  tAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAc                                                                                       >  2:6118875/1‑93 (MQ=255)

  tAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCAc                                                                                       >  1:598044/1‑93 (MQ=255)

     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    <  2:6815601/93‑1 (MQ=255)

     ggACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCAt                                                                                    >  2:869336/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  2:1716729/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  1:1923559/1‑93 (MQ=255)

       aCGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCt                                                                                  >  2:2616499/1‑93 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 >  2:9616563/1‑93 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 <  1:5052940/93‑1 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 >  1:529057/1‑93 (MQ=255)

        cGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTg                                                                                 <  1:8196017/93‑1 (MQ=255)

          aaGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCa                                                                               >  1:3341988/1‑93 (MQ=255)

          aaGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCa                                                                               >  1:8718049/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  2:3185048/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              >  2:9121637/1‑93 (MQ=255)

           aGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCAt                                                                              <  1:425318/93‑1 (MQ=255)

            ggTTTCTGACGACAGCGGGACTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGGTCAAGCACAGACCCCACCATCTGCAtt                                                                             >  2:8221063/1‑93 (MQ=255)

             gTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTc                                                                            >  1:9775824/1‑93 (MQ=255)

              tttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTcc                                                                           <  2:6481314/93‑1 (MQ=255)

               ttCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCt                                                                          <  2:4606290/93‑1 (MQ=255)

                   gacgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCt                                                                      >  1:2912107/1‑93 (MQ=255)

                    acgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTg                                                                     >  1:8323700/1‑93 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  2:7882740/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  1:9359106/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    >  1:1808060/1‑93 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    <  2:572423/93‑1 (MQ=255)

                     cgacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGa                                                                    >  1:5581606/1‑93 (MQ=255)

                      gacAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGAc                                                                   <  2:3275827/93‑1 (MQ=255)

                       acAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACg                                                                  >  1:5832457/1‑93 (MQ=255)

                        cAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGc                                                                 <  1:4686124/93‑1 (MQ=255)

                        cAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGc                                                                 >  2:4400091/1‑93 (MQ=255)

                         aGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCt                                                                >  2:4754238/1‑93 (MQ=255)

                           cGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCa                                                              >  2:8073499/1‑93 (MQ=255)

                            gggAATTTCAGTAAAAAGAAGCCAACCTTCATTTCACCTCCCCGATGCCCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCAc                                                             <  2:6312536/93‑1 (MQ=255)

                             ggAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACg                                                            >  1:2135882/1‑93 (MQ=255)

                              gAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGa                                                           >  1:3471509/1‑93 (MQ=255)

                              gAAATTCAATAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGa                                                           <  2:1109694/93‑1 (MQ=255)

                               aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGAc                                                          <  1:6239540/93‑1 (MQ=255)

                               aaTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGAc                                                          <  1:7202965/93‑1 (MQ=255)

                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  1:2482136/1‑93 (MQ=255)

                                aTTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACt                                                         >  1:2417562/1‑93 (MQ=255)

                                   tCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGAc                                                      >  1:5277387/1‑93 (MQ=255)

                                   tCAATAAAAAGAAGCCGAACTTCATTTACCATCCAAGAAGCGCTAAAGCACAGACCGCACCATCAGAATTCCTGTCTGACGCTAAagacagac                                                      <  1:8940458/93‑1 (MQ=255)

                                     aGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACgg                                                    >  2:1814782/1‑93 (MQ=255)

                                       tAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCt                                                  >  2:2318083/1‑93 (MQ=255)

                                        aaaaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCtt                                                 <  1:7699675/93‑1 (MQ=255)

                                          aaaGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCTCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCa                                               <  1:8105910/93‑1 (MQ=255)

                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc                                              <  2:9339825/93‑1 (MQ=255)

                                           aagaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCAc                                              <  1:4107610/93‑1 (MQ=255)

                                            agaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACt                                             >  1:7992231/1‑93 (MQ=255)

                                             gaagCCGACCTTCATTTCGCCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  2:5111568/93‑1 (MQ=255)

                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  1:8864968/93‑1 (MQ=255)

                                             gaagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTa                                            <  2:8930376/93‑1 (MQ=255)

                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           <  2:8469128/93‑1 (MQ=255)

                                              aagCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTaa                                           <  1:9186669/93‑1 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         <  1:9526047/93‑1 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         >  2:5974495/1‑93 (MQ=255)

                                                gCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGt                                         <  1:7453443/93‑1 (MQ=255)

                                                 ccGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGCCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTc                                        <  1:4020101/93‑1 (MQ=255)

                                                  cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcg                                       <  1:653121/93‑1 (MQ=255)

                                                  cGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcg                                       <  2:4223091/93‑1 (MQ=255)

                                                    aCCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTcgcg                                     >  1:1627492/1‑93 (MQ=255)

                                                      cTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCa                                   <  1:4421110/93‑1 (MQ=255)

                                                        tCATTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCAtt                                 <  2:8898614/93‑1 (MQ=255)

                                                         taTTTCACCTCCCCGATGCGCTCAAGCACAGGCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTc                                <  1:8405059/92‑1 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               >  2:10016228/1‑93 (MQ=255)

                                                          aTTTCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCa                               <  2:4316448/93‑1 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  2:2774181/93‑1 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  1:985419/93‑1 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              >  1:3401954/1‑93 (MQ=255)

                                                           tttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAg                              <  1:4216477/93‑1 (MQ=255)

                                                            ttCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgc                             <  2:4762480/93‑1 (MQ=255)

                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            <  2:7404028/93‑1 (MQ=255)

                                                             tCACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcg                            >  1:461621/1‑93 (MQ=255)

                                                              cACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc                           <  1:1447466/93‑1 (MQ=255)

                                                              cACCTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAgcgc                           >  2:1371361/1‑93 (MQ=255)

                                                                ccTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCg                         >  1:1654447/1‑93 (MQ=255)

                                                                ccTCCCCGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCg                         >  1:5653569/1‑93 (MQ=255)

                                                                 cTCCCCGATGCGCTCAAGCACAGCCCGCCCCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCgg                        <  1:6864922/93‑1 (MQ=255)

                                                                  cccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGa                       <  1:7750603/92‑1 (MQ=255)

                                                                   ccccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGaa                      <  2:9585091/93‑1 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  1:4990007/1‑93 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    <  2:1269345/93‑1 (MQ=255)

                                                                     ccGATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGc                    >  2:508029/1‑93 (MQ=255)

                                                                       gATGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTg                  >  1:7953753/1‑93 (MQ=255)

                                                                        aTGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTgg                 <  1:7319715/93‑1 (MQ=255)

                                                                         tGCGCTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGc                <  2:5381380/93‑1 (MQ=255)

                                                                            gcTCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGAGCCGGAAGCTGGCCTa             >  1:1034044/1‑93 (MQ=255)

                                                                             cTCAAGCACAGGCCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTaa            <  1:9510051/93‑1 (MQ=255)

                                                                              tCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAg           >  2:5873203/1‑93 (MQ=255)

                                                                              tCAAGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAg           <  1:2530080/93‑1 (MQ=255)

                                                                                aaGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGt         >  2:744628/1‑93 (MQ=255)

                                                                                 aGCACAGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTa        <  2:9503372/93‑1 (MQ=255)

                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGTAAGCTGGCCTAAGGTATc      <  2:4727/93‑1 (MQ=255)

                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc      <  1:8126994/93‑1 (MQ=255)

                                                                                   cacaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATc      >  2:6422173/1‑93 (MQ=255)

                                                                                    acaGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATcc     >  2:9068583/1‑93 (MQ=255)

                                                                                      aGACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGc   <  1:5414381/93‑1 (MQ=255)

                                                                                       gACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCa  >  1:3884118/1‑93 (MQ=255)

                                                                                       gACCGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGGATCCGCa  >  1:7651044/1‑93 (MQ=255)

GTTAAGGACGAAGGTTTCTGACGACAGCGGGAATTTCAGTAAAAAGAAGCCGACCTTCATTTCACCTCCCCGATGCGCTCAAGCACAGACTGCACCATCTGCATTCCTGTCTGACGCTCACGACTGACGGCTTCACTAAGTCGCGCATTCAGCGCCGGAAGCTGGCCTAAGGTATCCGCA  >  NC_000913/2115090‑2115269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`