BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,123,880	T→C	Q123R (CAG→CGG)	wcaH ←	GDP‑mannose mannosyl hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,123,880	0	T	C	100.0%	607.6 / NA	187	Q123R (CAG→CGG)	wcaH	GDP‑mannose mannosyl hydrolase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (89/98); total (89/98)

Only 100 of 200 total aligned reads displayed.

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG  >  NC_000913/2123790‑2123969

  ctcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTc                                                                                       <  1:2305468/93‑1 (MQ=255)

   tcAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCa                                                                                      <  2:6157327/93‑1 (MQ=255)

     aGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATc                                                                                    <  2:2450890/93‑1 (MQ=255)

      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   <  1:9750592/93‑1 (MQ=255)

      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   <  1:7524207/93‑1 (MQ=255)

      gCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATcc                                                                                   >  1:458369/1‑93 (MQ=255)

       cGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCg                                                                                  <  1:5655015/93‑1 (MQ=255)

        gagaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCgg                                                                                 >  1:5380118/1‑93 (MQ=255)

         agaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGc                                                                                <  2:6310222/93‑1 (MQ=255)

          gaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCa                                                                               <  1:5614484/93‑1 (MQ=255)

          gaAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCa                                                                               >  1:984513/1‑93 (MQ=255)

            aaaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAga                                                                             >  2:8532695/1‑92 (MQ=255)

             aaTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTa                                                                            <  2:1383950/93‑1 (MQ=255)

              aTAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCATCCATCGGTAATCGTCATGCCGCTCATCCCGCAGTaa                                                                           >  2:6136561/1‑93 (MQ=255)

               aaGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                          <  1:8305984/92‑1 (MQ=255)

               aaGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAAc                                                                          <  1:8166719/92‑1 (MQ=255)

                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                         <  2:6900057/93‑1 (MQ=255)

                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                         >  1:4995354/1‑93 (MQ=255)

                aGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACa                                                                         <  2:9795043/93‑1 (MQ=255)

                  gcgcGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGc                                                                       <  1:7829122/93‑1 (MQ=255)

                    gcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctc                                                                     <  2:791391/93‑1 (MQ=255)

                    gcgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctc                                                                     <  2:77691/93‑1 (MQ=255)

                     cgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctct                                                                    <  1:3391278/93‑1 (MQ=255)

                     cgGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGctct                                                                    <  2:5077996/93‑1 (MQ=255)

                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                   >  1:709907/1‑93 (MQ=255)

                      ggCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtctt                                                                   >  2:482025/1‑93 (MQ=255)

                        cTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCtcttct                                                                 <  2:8735361/93‑1 (MQ=255)

                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                >  1:5144746/1‑93 (MQ=255)

                         tGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTcttctt                                                                <  1:124945/93‑1 (MQ=255)

                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                               >  1:3158412/1‑93 (MQ=255)

                          gTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCttcttc                                                               <  1:1872178/93‑1 (MQ=255)

                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              >  1:6331907/1‑93 (MQ=255)

                           ttAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTcc                                                              <  2:9275090/93‑1 (MQ=255)

                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  1:10039944/93‑1 (MQ=255)

                             aGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGa                                                            <  2:5826023/93‑1 (MQ=255)

                               cagtaaCATTATCACTGGCTAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                          <  1:1438232/89‑1 (MQ=255)

                               aaTGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATa                                                          <  2:160354/92‑1 (MQ=255)

                                 tGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCCGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATAct                                                        >  2:7359235/1‑93 (MQ=255)

                                       ttATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAg                                                  >  1:2905975/1‑93 (MQ=255)

                                       ttATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAg                                                  >  2:1593690/1‑93 (MQ=255)

                                        tATCACTGGCGAGCAGCTCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                                                 <  1:9307718/93‑1 (MQ=255)

                                        tATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGc                                                 <  2:5402016/93‑1 (MQ=255)

                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                               >  1:1566505/1‑93 (MQ=255)

                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                               <  1:3611206/93‑1 (MQ=255)

                                          tCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGa                                               >  1:2775695/1‑93 (MQ=255)

                                           cACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaa                                              >  2:9367594/1‑93 (MQ=255)

                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  2:3365578/1‑93 (MQ=255)

                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  1:592385/1‑93 (MQ=255)

                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  1:2341827/1‑93 (MQ=255)

                                            aCTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaa                                             >  2:5796245/1‑93 (MQ=255)

                                             cgggCGAGCAGCGCGTCCGCCGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                            <  1:740731/91‑1 (MQ=255)

                                             cTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGaaaa                                            <  2:4223944/93‑1 (MQ=255)

                                              tGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAc                                           <  2:3439486/93‑1 (MQ=255)

                                                gagAGCAGCGCGTCCGACGTCAGCCAGCGGTAATTGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCg                                         >  2:6979787/3‑93 (MQ=255)

                                                 cGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGa                                        <  1:1169490/93‑1 (MQ=255)

                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                       >  1:6875012/1‑93 (MQ=255)

                                                  gAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAg                                       >  1:7006571/1‑93 (MQ=255)

                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGGAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                      >  1:3453893/1‑93 (MQ=255)

                                                   agcagcGCGTCCGACGTCAGCCAGCGGTAATCGGCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGc                                      <  1:9679368/93‑1 (MQ=255)

                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                     >  1:6841468/1‑93 (MQ=255)

                                                    gcagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGca                                     <  2:7624522/93‑1 (MQ=255)

                                                     cagcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcac                                    <  2:1278459/93‑1 (MQ=255)

                                                      agcGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacc                                   <  1:3774939/93‑1 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  1:3041949/1‑93 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  1:2219791/1‑93 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  2:3970634/1‑93 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcacca                                  >  2:4959644/1‑93 (MQ=255)

                                                       gcgcGTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAAACGAGcacca                                  >  2:3117436/1‑93 (MQ=255)

                                                        cgcgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGcaccac                                 <  1:6131060/93‑1 (MQ=255)

                                                          cgTCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACAt                               >  2:3592385/1‑93 (MQ=255)

                                                            tCCGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATag                             >  1:4272705/1‑93 (MQ=255)

                                                             ccGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGATGCGAAAACCGAGCACCACATagt                            >  2:5533153/1‑93 (MQ=255)

                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGCAAACCGAGCACCACATagtg                           >  2:1065994/1‑93 (MQ=255)

                                                              cGACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtg                           >  2:8042232/1‑93 (MQ=255)

                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                          <  1:5767844/93‑1 (MQ=255)

                                                               gACGTCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATagtga                          <  2:6753884/93‑1 (MQ=255)

                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      <  1:6730464/93‑1 (MQ=255)

                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      <  2:2020773/93‑1 (MQ=255)

                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      >  2:1843126/1‑93 (MQ=255)

                                                                   tCAGCCAGCGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtgg                      >  2:6825841/1‑93 (MQ=255)

                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  1:4417993/93‑1 (MQ=255)

                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  2:3533609/93‑1 (MQ=255)

                                                                     agccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTGCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAgtggtg                    <  1:8450548/93‑1 (MQ=255)

                                                                       ccagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaa                  >  1:4056249/1‑93 (MQ=255)

                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 >  1:9787849/1‑93 (MQ=255)

                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 >  2:2343055/1‑93 (MQ=255)

                                                                        cagcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGaaa                 >  1:8807098/1‑93 (MQ=255)

                                                                         agcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAAt                >  1:2707416/1‑93 (MQ=255)

                                                                          gcGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATc               <  1:8597420/93‑1 (MQ=255)

                                                                           cGGTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATcc              >  2:9062494/1‑93 (MQ=255)

                                                                            ggTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCg             >  2:2086596/1‑93 (MQ=255)

                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            <  2:2661189/93‑1 (MQ=255)

                                                                             gTAATCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGt            <  2:847635/93‑1 (MQ=255)

                                                                                 tCGTCATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCa        >  1:8777558/1‑93 (MQ=255)

                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  2:10044503/1‑93 (MQ=255)

                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  1:2470649/1‑93 (MQ=255)

                                                                                     cATGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAgaga    >  2:9842659/1‑93 (MQ=255)

                                                                                      aTGCCGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGaa   <  2:5990099/93‑1 (MQ=255)

                                                                                       tgccgcTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAg  >  2:2760068/1‑93 (MQ=255)

                                                                                       tgccgcTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAg  <  1:3014166/93‑1 (MQ=255)

TTCTCAGCGAGAAAATAGGCGCGGCTGTTAGCATGAACATTATCACTGGCGAGCAGCGCGTCCGACGTCAGCCAGCGGTAATCGTCATGCTGCTCATCCGGCAGTAACAGCTCTTCTTCCGATACTCTGAAGCGAAAACCGAGCACCACATAGTGAGTGGTGAAATCCGTGCCAGAGAAG  >  NC_000913/2123790‑2123969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`