BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,151,093	A→G	C20R (TGC→CGC)	b2073 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,151,093	0	A	G	99.7%	942.0 / ‑5.0	296	C20R (TGC→CGC)	b2073	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (157/137); total (158/138)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.11e-01

Only 100 of 304 total aligned reads displayed.

CATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTC  >  NC_000913/2151003‑2151182

catcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGAc                                                                                         >  2:9022264/1‑93 (MQ=255)

 atcaCGAAAGATAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACg                                                                                        <  1:116287/93‑1 (MQ=255)

  tcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGt                                                                                       <  1:1024614/93‑1 (MQ=255)

  tcaCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGt                                                                                       <  2:4465846/93‑1 (MQ=255)

  tcaCGAAAGGGAACCAATCCGGCGGTAAGTTCATTAATAGGTCTCCCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGTGCGACGt                                                                                       <  2:1631401/93‑1 (MQ=255)

    aCGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTgg                                                                                     >  2:4711608/1‑93 (MQ=255)

     cGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGt                                                                                    <  1:2847275/93‑1 (MQ=255)

       aaaGGTATCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTc                                                                                  <  2:8827238/93‑1 (MQ=255)

        aaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTcc                                                                                 <  1:6020961/93‑1 (MQ=255)

        aaGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTcc                                                                                 <  2:9083038/93‑1 (MQ=255)

         aGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCg                                                                                >  1:3253255/1‑93 (MQ=255)

          ggTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCgg                                                                               >  1:7674845/1‑93 (MQ=255)

          ggTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCgg                                                                               >  1:5156846/1‑93 (MQ=255)

           gTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCggg                                                                              >  2:7998714/1‑93 (MQ=255)

            tAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGt                                                                             <  2:9599211/93‑1 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            >  2:6402470/1‑93 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            <  1:1213843/93‑1 (MQ=255)

             aaCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGtt                                                                            >  1:4726716/1‑93 (MQ=255)

              aCCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTa                                                                           >  1:1599604/1‑93 (MQ=255)

               ccAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTAc                                                                          <  1:6215081/93‑1 (MQ=255)

               ccAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTAc                                                                          >  2:1313137/1‑93 (MQ=255)

                cAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACt                                                                         >  2:2003280/1‑93 (MQ=255)

                  aTCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTgg                                                                       >  1:4201432/1‑93 (MQ=255)

                  aTCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTgg                                                                       >  2:2537873/1‑93 (MQ=255)

                   tCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGc                                                                      <  1:3432965/93‑1 (MQ=255)

                       gCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCaaaa                                                                  >  1:8930505/1‑93 (MQ=255)

                        cGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAAt                                                                 <  1:9663409/93‑1 (MQ=255)

                        cGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAAt                                                                 <  1:638718/93‑1 (MQ=255)

                        cGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAAt                                                                 >  2:51214/1‑93 (MQ=255)

                          ttAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCg                                                               >  1:2570424/1‑93 (MQ=255)

                           tAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGc                                                              >  1:1966942/1‑93 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             <  2:967265/93‑1 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             >  2:4581544/1‑93 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             <  1:3925486/93‑1 (MQ=255)

                            aaGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCt                                                             <  1:8749424/93‑1 (MQ=255)

                              gTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtgg                                                           <  2:3524761/93‑1 (MQ=255)

                              gTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtgg                                                           >  2:2898802/1‑93 (MQ=255)

                              gTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtgg                                                           <  1:3546663/93‑1 (MQ=255)

                               ttCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggt                                                          <  2:7202575/93‑1 (MQ=255)

                                tCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtg                                                         >  1:6962560/1‑93 (MQ=255)

                                 cATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                        >  1:4561033/1‑93 (MQ=255)

                                 cATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCtggtgg                                                        <  2:3817715/93‑1 (MQ=255)

                                  aTTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGc                                                       <  2:5279883/93‑1 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      >  1:2429380/1‑93 (MQ=255)

                                   ttAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCa                                                      >  2:1519657/1‑93 (MQ=255)

                                    tAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCaa                                                     >  2:1062124/1‑93 (MQ=255)

                                     aaTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCaaa                                                    <  2:3312861/93‑1 (MQ=255)

                                      aTAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAAc                                                   >  2:4090955/1‑93 (MQ=255)

                                      aTAGCTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAAc                                                   <  2:2254761/93‑1 (MQ=255)

                                       tAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACg                                                  >  2:936689/1‑93 (MQ=255)

                                           tCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAt                                              <  2:8733729/93‑1 (MQ=255)

                                             tACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGAttt                                            >  2:6726020/1‑93 (MQ=255)

                                              aCCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTg                                           >  1:3009077/1‑93 (MQ=255)

                                               ccGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGt                                          >  2:5988701/1‑93 (MQ=255)

                                               ccGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTCATTTGt                                          >  1:8156726/1‑93 (MQ=255)

                                                cGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGtt                                         <  2:3049411/93‑1 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        <  2:8268531/93‑1 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        >  1:6252966/1‑93 (MQ=255)

                                                 gTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                        <  1:941295/93‑1 (MQ=255)

                                                  ttCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCg                                       >  1:5266719/1‑93 (MQ=255)

                                                   tCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGc                                      >  2:7008465/1‑93 (MQ=255)

                                                    cATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCt                                     >  1:9756042/1‑93 (MQ=255)

                                                     aTAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTc                                    >  1:5465562/1‑93 (MQ=255)

                                                      tAGAGCCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCa                                   <  1:136746/93‑1 (MQ=255)

                                                       aGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGTTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCAt                                  <  2:561847/93‑1 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 >  1:5960375/1‑93 (MQ=255)

                                                        gAACCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATa                                 >  1:8109452/1‑93 (MQ=255)

                                                         aaCTAGAATCATCCAGCAGCAAAAAGCATTGGCGATTTGGTTCCGGGATACTGGCAAAATCGCTGGTGGCAAACGCGATATGTTCGCTCATaa                                >  2:9254938/1‑93 (MQ=255)

                                                         aaCCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATaa                                >  1:7516251/1‑93 (MQ=255)

                                                          aCCAGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAg                               <  2:5771130/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                            <  1:9525204/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                            <  1:517375/93‑1 (MQ=255)

                                                             aGAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGaaa                            >  1:834823/1‑93 (MQ=255)

                                                              gAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAAt                           <  1:801486/93‑1 (MQ=255)

                                                              gAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAAt                           <  1:9178261/93‑1 (MQ=255)

                                                              gAAACATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAAt                           >  2:3922594/1‑93 (MQ=255)

                                                               aaaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATc                          >  2:632087/1‑93 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTTATTTGTTCGCTCATAAGAAATcc                         >  1:5728432/1‑93 (MQ=255)

                                                                aaCATCCAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATct                         >  1:4830304/1‑92 (MQ=255)

                                                                     ccAGCAGCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCt                    <  2:4263078/93‑1 (MQ=255)

                                                                      cagcagCAAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCtt                   <  1:5848711/93‑1 (MQ=255)

                                                                        gcagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTag                 <  1:1516716/93‑1 (MQ=255)

                                                                        gcagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTag                 >  2:7911265/1‑93 (MQ=255)

                                                                         cagcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTaga                >  2:6798908/1‑93 (MQ=255)

                                                                           gcaAAATGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAgaga              <  2:6114895/93‑1 (MQ=255)

                                                                           gcaAAATGCAGGGGCGACGTGGCTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAgaga              >  2:7039925/1‑93 (MQ=255)

                                                                             aaaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAt            >  1:2085315/1‑93 (MQ=255)

                                                                              aaaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAtt           <  1:2631340/93‑1 (MQ=255)

                                                                               aaTGCAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAAttt          >  1:9143673/1‑92 (MQ=255)

                                                                                   cAGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGa      <  2:852160/93‑1 (MQ=255)

                                                                                    aGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAg     <  2:8691152/93‑1 (MQ=255)

                                                                                    aGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAg     >  1:6669558/1‑93 (MQ=255)

                                                                                    aGGGGCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAg     <  2:2721254/93‑1 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    >  2:1829071/1‑93 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    >  2:740488/1‑93 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    <  1:8475201/93‑1 (MQ=255)

                                                                                     ggggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAgg    >  2:5367961/1‑93 (MQ=255)

                                                                                      gggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAggc   >  1:2374275/1‑92 (MQ=255)

                                                                                      gggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAACCCTTTCTTAGAGAATTATCGAGGt   >  1:7819349/1‑93 (MQ=255)

                                                                                       ggCGACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc  <  1:7215726/93‑1 (MQ=255)

CATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTC  >  NC_000913/2151003‑2151182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`