Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 260,932 C→T T69M (ACG→ATG)  proA → gamma‑glutamylphosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913260,9320CT99.4% 1188.2 / ‑4.7 327T69M (ACG→ATG) proAgamma‑glutamylphosphate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base T (126/199);  total (126/201)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 338 total aligned reads displayed.

AAGCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGACGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTAC  >  NC_000913/260840‑261021
                                                                                            |                                                                                         
aaacGATATCATCCTCAACGCAAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGAc                                                                                           <  1:6958377/90‑1 (MQ=255)
  gCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGc                                                                                         <  1:6161146/93‑1 (MQ=255)
   ggAAATCACCCTGAACGCAAACCCCCAGGATTTTGCTAACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGACGcc                                                                                        <  2:8091079/92‑1 (MQ=255)
    gAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGccc                                                                                       <  2:4588137/93‑1 (MQ=255)
    gAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGccc                                                                                       >  2:23624/1‑93 (MQ=255)
    gAAATCACCCTCAACGCTAACGCCCAGGATGTTGCTGGCGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGccc                                                                                       <  1:7870870/93‑1 (MQ=255)
     aaaTCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCg                                                                                      <  1:4621082/93‑1 (MQ=255)
      aaTCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGc                                                                                     <  1:8319017/93‑1 (MQ=255)
        tcatcCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCAc                                                                                   >  1:1546661/1‑93 (MQ=255)
         cacccTCAAGGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACg                                                                                  <  1:89194/90‑1 (MQ=255)
          atcCTCAACGCTAGCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACgg                                                                                 <  1:7150599/93‑1 (MQ=255)
          atcCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACgg                                                                                 <  2:6909600/93‑1 (MQ=255)
           tcCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGc                                                                                >  2:1552641/1‑93 (MQ=255)
            ccTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCt                                                                               <  1:1200514/93‑1 (MQ=255)
              tCAACGCTACCGCCCAGGATGTTGCTGCCGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGa                                                                             <  2:7321172/93‑1 (MQ=255)
              tCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGa                                                                             >  2:5320876/1‑93 (MQ=255)
               cAACGCTACCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGaa                                                                            <  2:9722322/93‑1 (MQ=255)
                aaCGCTACCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGaaa                                                                           <  1:5801514/93‑1 (MQ=255)
                  cGCTACCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAgg                                                                         <  2:7156046/93‑1 (MQ=255)
                  cGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAgg                                                                         >  2:5478258/1‑93 (MQ=255)
                   gCTACCGCCGAGAATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGc                                                                        <  2:9634581/93‑1 (MQ=255)
                    cTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCa                                                                       <  2:9387147/93‑1 (MQ=255)
                     tAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCAt                                                                      <  2:7055374/93‑1 (MQ=255)
                      aaCGCCTAGGATGTTGTTAGCCGGGGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCAtt                                                                     <  1:3940757/93‑1 (MQ=255)
                      aaCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCAtt                                                                     >  2:3799829/1‑93 (MQ=255)
                      aaCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCAtt                                                                     >  1:1038142/1‑93 (MQ=255)
                       aCGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTg                                                                    >  2:607823/1‑93 (MQ=255)
                        cGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGc                                                                   <  2:3481457/93‑1 (MQ=255)
                           ccAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcga                                                                >  2:129931/1‑93 (MQ=255)
                           ccAGGATGTTCCTGACCCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcga                                                                <  1:5319082/93‑1 (MQ=255)
                           ccAGGATGTCTCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcga                                                                <  1:27159/93‑1 (MQ=255)
                            cAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcgac                                                               >  1:7503387/1‑93 (MQ=255)
                              ggttGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcgacga                                                             <  2:64077/90‑1 (MQ=255)
                              ggATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcgacga                                                             >  1:13101/1‑93 (MQ=255)
                              ggATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCcgacga                                                             >  1:5606082/1‑93 (MQ=255)
                               gATGTTGCTGCCGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGAt                                                            <  2:2092339/93‑1 (MQ=255)
                               gATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGAt                                                            <  2:2744286/93‑1 (MQ=255)
                               gATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGAt                                                            <  1:4394192/93‑1 (MQ=255)
                               gATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGAt                                                            <  1:1002856/93‑1 (MQ=255)
                               gATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTCGACCGTCTGGCACTGATGCCCGCCCGGCGAAAAGGCATTGCGGACCAt                                                            >  1:3255266/1‑93 (MQ=255)
                                aTGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATg                                                           <  2:7664542/93‑1 (MQ=255)
                                 tGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGt                                                          >  1:7197138/1‑93 (MQ=255)
                                  tttGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTa                                                         <  1:2942119/92‑1 (MQ=255)
                                  gTTGCTGCCGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTa                                                         <  2:4027577/93‑1 (MQ=255)
                                  gTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGTATTGGCGACGATGTa                                                         >  2:852503/1‑93 (MQ=255)
                                   ttGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTAc                                                        <  1:7625881/93‑1 (MQ=255)
                                   ttGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTAc                                                        <  1:3418279/93‑1 (MQ=255)
                                    tGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACg                                                       <  2:4546350/93‑1 (MQ=255)
                                     gCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGt                                                      >  1:8113775/1‑93 (MQ=255)
                                     gCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGt                                                      >  1:5947638/1‑93 (MQ=255)
                                      cTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTc                                                     <  1:9227721/93‑1 (MQ=255)
                                      cTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTc                                                     <  1:7554519/93‑1 (MQ=255)
                                      cTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTc                                                     >  1:3731966/1‑93 (MQ=255)
                                       ggACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCa                                                    <  1:6269579/92‑1 (MQ=255)
                                        gACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAg                                                   <  1:6397842/93‑1 (MQ=255)
                                        gACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAg                                                   <  1:252237/93‑1 (MQ=255)
                                         gcgcgcgAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAgg                                                  <  2:167195/92‑1 (MQ=255)
                                         ccgcgcgAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAgg                                                  <  2:5734587/92‑1 (MQ=255)
                                          cgcgcgAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAggg                                                 >  1:9305964/1‑92 (MQ=255)
                                           gcgcgAGCCTATGGCCTTAGCGAAGCGGTGCTGGACCGTCTGGCACTGATCCCCGCACGTCGGAAAGGCATTGCCGACGATGTACGTCAGgtg                                                <  2:8558349/93‑1 (MQ=255)
                                           gcgcgAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGgtg                                                <  1:8535518/93‑1 (MQ=255)
                                            cgcgAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGgtgt                                               >  2:1265243/1‑93 (MQ=255)
                                              cgAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAGAGGCATTGCCGACGATGTACGTCAGGTGTGc                                             <  2:2493400/93‑1 (MQ=255)
                                               gAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCa                                            >  2:7388199/1‑93 (MQ=255)
                                                aGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGtgtggac                                           >  2:4163555/1‑90 (MQ=255)
                                                aGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCaa                                           <  1:6720258/93‑1 (MQ=255)
                                                aGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGACGCCGATGTACGTCAGGTGTGCac                                           >  2:6338921/1‑92 (MQ=255)
                                                  ccAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAAcc                                         <  1:956933/93‑1 (MQ=255)
                                                      tGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGc                                     >  2:8885327/1‑93 (MQ=255)
                                                      tGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGc                                     <  2:9876020/93‑1 (MQ=255)
                                                       ggCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGcc                                    >  2:4801872/1‑93 (MQ=255)
                                                          cTTAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGAt                                 <  1:3489872/93‑1 (MQ=255)
                                                           ttAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATc                                <  1:7772990/93‑1 (MQ=255)
                                                           ttAGCGAAGCGATGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATc                                >  1:4105172/1‑93 (MQ=255)
                                                             agcgaagcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCg                              <  2:3325279/93‑1 (MQ=255)
                                                              gcgaagcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCgg                             <  1:4951306/93‑1 (MQ=255)
                                                              gcgaagcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCgg                             <  2:1041672/93‑1 (MQ=255)
                                                               cgaagcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGt                            <  2:2557111/93‑1 (MQ=255)
                                                                 aagcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTgg                          <  2:3254332/93‑1 (MQ=255)
                                                                 aagcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTgg                          <  2:3652628/93‑1 (MQ=255)
                                                                   gcgaTGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGgggggg                        >  2:7262055/1‑93 (MQ=255)
                                                                       tGCTTGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAgg                    >  2:8374439/1‑93 (MQ=255)
                                                                          ttGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTaa                 <  1:9835055/93‑1 (MQ=255)
                                                                           tGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAAt                >  2:9511067/1‑93 (MQ=255)
                                                                           tGACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGGGGGGCAGGTAAt                >  1:2096235/1‑93 (MQ=255)
                                                                            gACCGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTATGCAACCTCGCCGATCCGGTGGGGCAGGTAATc               <  1:2281619/93‑1 (MQ=255)
                                                                              ccGTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGa             <  1:5857937/93‑1 (MQ=255)
                                                                                gTCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATg           >  1:3325679/1‑93 (MQ=255)
                                                                                 tCTGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATgg          >  1:5252670/1‑93 (MQ=255)
                                                                                   tGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATggcg        <  2:783429/93‑1 (MQ=255)
                                                                                   tGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATggcg        >  2:6722455/1‑93 (MQ=255)
                                                                                   tGGCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATggcg        <  2:1186004/93‑1 (MQ=255)
                                                                                    ggCACTGATGCCCGCACGGCTGAATGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATggcgg       >  2:7542136/1‑93 (MQ=255)
                                                                                    ggCACTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATggcgg       <  2:8303531/93‑1 (MQ=255)
                                                                                       aCTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGt    <  2:4970665/93‑1 (MQ=255)
                                                                                        cTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTa   <  1:4525820/93‑1 (MQ=255)
                                                                                        cTGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTa   <  1:1830922/93‑1 (MQ=255)
                                                                                         tGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTAc  <  2:433445/93‑1 (MQ=255)
                                                                                         tGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTAc  >  1:880680/1‑93 (MQ=255)
                                                                                         tGATGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTAc  <  2:2948882/93‑1 (MQ=255)
                                                                                            |                                                                                         
AAGCGAAATCATCCTCAACGCTAACGCCCAGGATGTTGCTGACGCGCGAGCCAATGGCCTTAGCGAAGCGATGCTTGACCGTCTGGCACTGACGCCCGCACGGCTGAAAGGCATTGCCGACGATGTACGTCAGGTGTGCAACCTCGCCGATCCGGTGGGGCAGGTAATCGATGGCGGCGTAC  >  NC_000913/260840‑261021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑