Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,208,331 A→G S404S (TCA→TCG)  b2122 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,208,3310AG99.5% 637.0 / ‑5.6 205S404S (TCA→TCG) b2122hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (64/140);  total (65/140)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 235 total aligned reads displayed.

CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208243‑2208420
                                                                                         |                                                                                         
  cgAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAagca                                                                                     <  2:159345/93‑1 (MQ=255)
   gAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAagcag                                                                                    >  1:9124014/1‑93 (MQ=255)
    aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑AAATTTTTTCGGCagcagc                                                                                   >  2:8218874/1‑93 (MQ=255)
    aaGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑AAATTGGTTCGCAagcagc                                                                                   >  2:9414601/1‑93 (MQ=255)
     aGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAacagca                                                                                  >  2:9029757/1‑93 (MQ=255)
      gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGG‑ACAATTTTTCGCAAGCCGCaa                                                                                 >  2:2441801/1‑93 (MQ=255)
      gTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaa                                                                                 <  2:7000662/93‑1 (MQ=255)
        gAACAGGACACCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaaaa                                                                               <  2:6300929/93‑1 (MQ=255)
        gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaaaa                                                                               <  1:3137874/93‑1 (MQ=255)
        gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGCTTCGCAAGCAGCaaaa                                                                               <  2:8112529/93‑1 (MQ=255)
        gAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAATTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCaaaa                                                                               <  1:4940581/93‑1 (MQ=255)
         aaCAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAACCACCAAAAc                                                                              >  1:8532488/1‑93 (MQ=255)
            cggAAAACTCCCCTGCAGCTACGCGTCGGCTGTTTAATTTTCAGGCTGGCGCACTGGCCGGCGGGG‑AAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  2:2836198/92‑1 (MQ=255)
            aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCa                                                                           >  2:934382/1‑93 (MQ=255)
            aGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCa                                                                           <  1:8740051/93‑1 (MQ=255)
                aaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGc                                                                       <  1:6141478/93‑1 (MQ=255)
                aaaCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGc                                                                       <  2:893898/93‑1 (MQ=255)
                  aCTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCg                                                                     <  2:8598364/93‑1 (MQ=255)
                   cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  1:8115187/93‑1 (MQ=255)
                   cTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGa                                                                    <  2:9747114/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCATTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  2:3382718/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  1:5642769/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAt                                                                   <  1:7946284/93‑1 (MQ=255)
                    tCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGGTTCGCCAGCAGCAAAAACCAGTGCCCAt                                                                   >  1:4731511/1‑93 (MQ=255)
                      aCCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATgg                                                                 >  2:1093529/1‑93 (MQ=255)
                       ccGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGc                                                                <  2:6024162/93‑1 (MQ=255)
                         ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              >  1:1066914/1‑93 (MQ=255)
                         ggCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGa                                                              <  2:6669123/93‑1 (MQ=255)
                              cTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑GAAATGTTTCGCAAGCAGCAAAAGGCGGTGCCGAGGGGGAgctgg                                                         >  1:7556741/1‑92 (MQ=255)
                               tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGCgctgct                                                        >  2:8366108/1‑93 (MQ=255)
                               tACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAgctgct                                                        >  1:329743/1‑93 (MQ=255)
                                aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGctgctg                                                       <  1:3869708/93‑1 (MQ=255)
                                aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGctgctg                                                       <  1:3852785/93‑1 (MQ=255)
                                aCGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGAGGGCGAGctgctg                                                       >  2:4365723/1‑93 (MQ=255)
                                 cgcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGt                                                      >  2:8571658/1‑93 (MQ=255)
                                  gcgTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGCAAAATTGTTT‑GCAAGCAGCAAAACGCCGTGCCGACGGGGAGCTGCTGtt                                                     >  1:5576136/1‑93 (MQ=255)
                                  gcgTCGGCTGTTTAGTGTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGtt                                                     <  1:6632416/93‑1 (MQ=255)
                                     tCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGAAGCAAAACGCAGTGCCGATTGCGAGCTGCTGTTAGc                                                  >  1:6880025/1‑93 (MQ=255)
                                      cGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCt                                                 <  2:5676180/93‑1 (MQ=255)
                                       ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCCAAACGCAGTGCCGATGGCGGGCTGCTGTTAGCTa                                                >  1:6500426/1‑93 (MQ=255)
                                       ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  1:3593222/1‑93 (MQ=255)
                                       ggCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTa                                                >  2:7206330/1‑93 (MQ=255)
                                        gCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAc                                               <  1:2496114/93‑1 (MQ=255)
                                         cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGGAAGCAGCAAAACGCAGGGCCGATGGCGAGCTGCTGTTAGCTAcc                                              >  2:3860993/1‑93 (MQ=255)
                                         cTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAcc                                              <  1:723960/93‑1 (MQ=255)
                                          tGTTTAGTTTTCAGGCTGGCGCACTGGCGTGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  1:1477972/93‑1 (MQ=255)
                                          tGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTAccc                                             <  2:3537189/93‑1 (MQ=255)
                                           gattAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCg                                            <  2:4761013/91‑1 (MQ=255)
                                           gTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCCGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCg                                            >  1:4549502/1‑93 (MQ=255)
                                           gTTTAGTTTTCAGGCTCGCGCACTGGCGGGCGGGG‑AAATTGATTTGCTAGCAGCAAAACGCAGCGCCGATCGCGTGCTGCCGTTAGATACCCg                                            >  2:2122568/1‑93 (MQ=255)
                                              tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  1:5278764/93‑1 (MQ=255)
                                              tAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCaa                                         <  1:4677043/93‑1 (MQ=255)
                                                gTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAAcc                                       <  2:6420610/93‑1 (MQ=255)
                                                  tttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     <  1:4046656/93‑1 (MQ=255)
                                                  tttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGc                                     >  1:538882/1‑93 (MQ=255)
                                                   ttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    >  1:1898626/1‑93 (MQ=255)
                                                   ttCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCt                                    >  1:9365204/1‑93 (MQ=255)
                                                     cAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTa                                  <  1:6401934/93‑1 (MQ=255)
                                                     cAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTa                                  <  2:9694657/93‑1 (MQ=255)
                                                       ggCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAag                                >  2:4276407/1‑93 (MQ=255)
                                                        gCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               >  2:2619308/1‑93 (MQ=255)
                                                        gCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagc                               <  2:7572872/93‑1 (MQ=255)
                                                         cTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTATCCGCAACCGCTTAagca                              >  2:10017071/1‑93 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            >  1:2365436/1‑93 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  1:2438058/93‑1 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            >  2:4324313/1‑93 (MQ=255)
                                                           ggCGCACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  2:9911846/93‑1 (MQ=255)
                                                           ggCGCACGGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAagcagc                            <  2:9628654/93‑1 (MQ=255)
                                                            gcgcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCg                           >  2:4412351/1‑93 (MQ=255)
                                                             cgcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:1807500/93‑1 (MQ=255)
                                                             cgcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGt                          <  2:5846753/93‑1 (MQ=255)
                                                              gcACTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGtt                         >  2:4102802/1‑93 (MQ=255)
                                                                aCTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAAAGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgt                       <  2:7477450/93‑1 (MQ=255)
                                                                 cTGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTtgtg                      <  1:3933004/93‑1 (MQ=255)
                                                                  tGGCGGGCGGGC‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGc                     >  2:4751612/1‑93 (MQ=255)
                                                                      gggcgggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCt                 <  2:5901034/93‑1 (MQ=255)
                                                                       ggcgggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  2:117693/1‑93 (MQ=255)
                                                                       ggcgggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTg                >  1:5098808/1‑93 (MQ=255)
                                                                          gggc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCg             >  2:5006326/1‑93 (MQ=255)
                                                                            gc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           <  2:9124013/93‑1 (MQ=255)
                                                                            gc‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGcc           <  1:5887784/93‑1 (MQ=255)
                                                                             c‑AAATTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCt          <  1:222496/93‑1 (MQ=255)
                                                                               aaaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTg         <  2:8744739/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        >  1:1792506/1‑93 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:3068104/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  2:3274506/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:9058443/93‑1 (MQ=255)
                                                                                aaTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGa        <  1:4092064/93‑1 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  1:6272020/93‑1 (MQ=255)
                                                                                 aTTGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGAt       <  2:3126578/93‑1 (MQ=255)
                                                                                  ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      >  2:8049913/1‑93 (MQ=255)
                                                                                  ttGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATg      <  2:7784798/93‑1 (MQ=255)
                                                                                   tctttCGCAAGCAGCAAAGCGCAGTGCCGATGGCGATCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     <  2:1836515/91‑1 (MQ=255)
                                                                                   tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     >  2:9002739/1‑93 (MQ=255)
                                                                                   tGTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGc     <  1:2927959/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCTGCTGTCGCCTGATGcc    <  2:9556962/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  1:6758241/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  1:3564898/93‑1 (MQ=255)
                                                                                    gTTTCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGcc    <  2:6791176/93‑1 (MQ=255)
                                                                                      ttCGCAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTg  <  1:1343194/93‑1 (MQ=255)
                                                                                         |                                                                                         
CGCGAAGTGAACAGGAAAACTCACCGGCAGCTACGCGTCGGCTGTTTAGTTTTCAGGCTGGCGCACTGGCGGGCGGGC‑AAATTGTTTCACAAGCAGCAAAACGCAGTGCCGATGGCGAGCTGCTGTTAGCTACCCGCAACCGCTTAAGCAGCGTTGTGCCGCTGTCGCCTGATGCCTG  >  NC_000913/2208243‑2208420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑