BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,741	T→C	D76G (GAC→GGC)	yejK ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,741	0	T	C	98.3%	896.7 / 2.4	293	D76G (GAC→GGC)	yejK	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (151/135); total (156/137)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-01

Only 100 of 314 total aligned reads displayed.

CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281651‑2281830

gaaaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGccc                                                                                         <  1:8680368/92‑1 (MQ=255)

  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctc                                                                                       >  2:3796314/1‑93 (MQ=255)

  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctc                                                                                       <  1:3632965/93‑1 (MQ=255)

  aaaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGAGGCTAAATGCCAGGAAGCCctc                                                                                       >  1:1818192/1‑93 (MQ=255)

   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGGGGCTAAATGCCAGGAAGCCctct                                                                                      >  2:5511908/1‑93 (MQ=255)

   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCctct                                                                                      >  1:8799663/1‑93 (MQ=255)

   aaCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCctct                                                                                      <  2:3879826/93‑1 (MQ=255)

    aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                     <  1:7882446/93‑1 (MQ=255)

    aCAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCtt                                                                                     <  2:7450580/93‑1 (MQ=255)

         aCGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGccc                                                                                <  1:5355403/93‑1 (MQ=255)

          cGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCt                                                                               <  2:3570032/93‑1 (MQ=255)

          cGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCt                                                                               >  2:5178449/1‑93 (MQ=255)

            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             >  2:8135753/1‑93 (MQ=255)

            aaTCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGa                                                                             <  2:6343467/93‑1 (MQ=255)

              tCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACg                                                                           >  1:7644673/1‑93 (MQ=255)

                cACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCt                                                                         >  2:3008734/1‑93 (MQ=255)

                  ccGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGt                                                                       <  2:3678417/93‑1 (MQ=255)

                   cGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTa                                                                      <  2:4632327/93‑1 (MQ=255)

                     tCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAg                                                                    >  1:3309721/1‑93 (MQ=255)

                      cAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgc                                                                   <  2:4939029/93‑1 (MQ=255)

                       aGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcg                                                                  >  1:1496693/1‑93 (MQ=255)

                        gCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAgcgc                                                                 >  1:1925290/1‑93 (MQ=255)

                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                >  2:2033599/1‑93 (MQ=255)

                         cAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCa                                                                <  1:9400799/93‑1 (MQ=255)

                          aaaaGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAg                                                               <  1:8366300/93‑1 (MQ=255)

                             aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            >  1:6548693/1‑93 (MQ=255)

                             aGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGt                                                            <  2:9212146/93‑1 (MQ=255)

                             aGGATATTTCGCTAATTCGTCACGCAGGC‑GCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  1:1022760/93‑1 (MQ=255)

                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           <  1:141307/93‑1 (MQ=255)

                              ggATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTc                                                           >  2:1629347/1‑93 (MQ=255)

                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          >  2:7507399/1‑93 (MQ=255)

                               gATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCt                                                          <  1:2770950/93‑1 (MQ=255)

                                gtatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         <  1:6203063/92‑1 (MQ=255)

                                atatTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTg                                                         <  1:9947543/93‑1 (MQ=255)

                                   tttCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTgcgc                                                      <  1:9874876/93‑1 (MQ=255)

                                     tCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCa                                                    <  2:6511818/93‑1 (MQ=255)

                                     tCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCAGTAAGCGCAGCGTCTGCGCCa                                                    <  2:2637637/93‑1 (MQ=255)

                                      cGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAg                                                   <  1:5017312/93‑1 (MQ=255)

                                       gCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGt                                                  >  1:5753771/1‑93 (MQ=255)

                                        cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGtt                                                 <  1:924075/93‑1 (MQ=255)

                                        cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGtt                                                 <  2:9322252/93‑1 (MQ=255)

                                        cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGtt                                                 >  1:7828522/1‑93 (MQ=255)

                                        cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGtt                                                 >  2:1439250/1‑93 (MQ=255)

                                        cTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGtt                                                 >  1:6677961/1‑93 (MQ=255)

                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                >  1:3276425/1‑93 (MQ=255)

                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                <  1:9594265/93‑1 (MQ=255)

                                         tAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTc                                                >  2:437418/1‑93 (MQ=255)

                                          aaTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCa                                               <  1:7761690/93‑1 (MQ=255)

                                           aTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAc                                              >  2:707483/1‑93 (MQ=255)

                                           aTTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAc                                              <  1:9073551/93‑1 (MQ=255)

                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                             >  1:1172972/1‑93 (MQ=255)

                                            ttCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCAct                                             >  1:208948/1‑93 (MQ=255)

                                             tCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActc                                            <  1:4702563/93‑1 (MQ=255)

                                             tCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActc                                            >  1:7356224/1‑93 (MQ=255)

                                              cGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActct                                           >  1:5645154/1‑93 (MQ=255)

                                               gTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctc                                          >  2:94238/1‑93 (MQ=255)

                                               gTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCActctc                                          >  1:4085786/1‑93 (MQ=255)

                                                 cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                        <  1:3799454/93‑1 (MQ=255)

                                                 cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                        <  2:5685532/93‑1 (MQ=255)

                                                 cACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCtt                                        <  2:533522/93‑1 (MQ=255)

                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCTCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       <  2:1807772/93‑1 (MQ=255)

                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       >  2:7911140/1‑93 (MQ=255)

                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       >  2:7181637/1‑93 (MQ=255)

                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       <  2:2778472/93‑1 (MQ=255)

                                                  aCGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTc                                       <  1:1656833/93‑1 (MQ=255)

                                                   cGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCg                                      >  2:1132907/1‑93 (MQ=255)

                                                      aGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTCAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTa                                   >  1:9678369/1‑93 (MQ=255)

                                                      aGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTa                                   >  2:1930904/1‑93 (MQ=255)

                                                       ggcgacCGGTTACCGAGCGGCTAAATGCCAGGACGCCCTCTTCACCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGATCACACTCTTAACTaa                                  <  2:8992890/93‑1 (MQ=255)

                                                          ggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACa                               <  2:4858150/93‑1 (MQ=255)

                                                          ggcCGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACa                               <  2:2718379/93‑1 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGATGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            <  2:837058/93‑1 (MQ=255)

                                                             cGGTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGc                            >  1:8465641/1‑93 (MQ=255)

                                                              ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                           <  2:3963757/93‑1 (MQ=255)

                                                              ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                           <  1:3422227/93‑1 (MQ=255)

                                                              ggTTGCCGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGcc                           >  1:5825300/1‑93 (MQ=255)

                                                                   ccGCGCGGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAg                      >  1:8487110/1‑93 (MQ=255)

                                                                    cgcgcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGc                     <  1:6394579/93‑1 (MQ=255)

                                                                       gcgGCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCttt                  <  1:129194/93‑1 (MQ=255)

                                                                         ggCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAt                >  2:5060700/1‑93 (MQ=255)

                                                                          gCTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt               >  2:9132973/1‑93 (MQ=255)

                                                                          gCTAAAAGCGAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGGGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtt               <  2:3221121/93‑1 (MQ=255)

                                                                           cTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt              <  1:7070831/93‑1 (MQ=255)

                                                                           cTAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCGGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttt              <  1:3104446/93‑1 (MQ=255)

                                                                            tAAATGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             >  1:426936/1‑93 (MQ=255)

                                                                            tAAATGCCAGAAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             <  2:5890177/93‑1 (MQ=255)

                                                                            tAAATGCAAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGAAGCGTCTGCGCCAGTTCCCTCTCTTCGCTAAACAAGCCGTAAGCTTTAtttt             <  1:444066/93‑1 (MQ=255)

                                                                             aaaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTAttttt            >  1:5143823/1‑93 (MQ=255)

                                                                              aaggCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg           <  1:7541535/90‑1 (MQ=255)

                                                                              aaTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTg           <  1:8245790/93‑1 (MQ=255)

                                                                               aTGCCAGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTgg          >  2:8601012/1‑93 (MQ=255)

                                                                                  ccAGGAAGCCCTCTTCCCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGgcgc       <  1:69097/93‑1 (MQ=255)

                                                                                    aGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta     <  1:6807220/93‑1 (MQ=255)

                                                                                    aGGAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCta     >  1:4403168/1‑93 (MQ=255)

                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat    <  2:4433330/93‑1 (MQ=255)

                                                                                     ggAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat    >  1:8074409/1‑93 (MQ=255)

                                                                                     ggAAGCCCTCTTCGCCCTGACGCGGTAGGCGCGGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtat    <  1:5511298/93‑1 (MQ=255)

                                                                                      gAAGCCTTCTCCGCCCTGACGCTGAAAGCGCCGCGTCTGCGCCATTTCACTCTCTTCGCTAAAAAAGCCGTAAGCTTTATTTTTGGCGCtata   <  2:6689220/93‑1 (MQ=255)

                                                                                      gAAGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCtata   >  1:2139463/1‑93 (MQ=255)

                                                                                       aaGCCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAg  >  2:8833773/1‑93 (MQ=255)

CAAAACAGCACGAATCCACCGTCAGCAAAAGGATATTTCGCTAATTCGTCACGCAGGCGGCCGGTTGCCGCGCGGCTAAATGCCAGGAAGTCCTCTTCGCCCTGACGCTGTAAGCGCAGCGTCTGCGCCAGTTCACTCTCTTCGCTAAACAAGCCGTAAGCTTTATTTTTGGCGCTATAG  >  NC_000913/2281651‑2281830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`