BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,306,256	T→C	E127G (GAA→GGA)	yojI ←	putative ATP‑binding component of a transport system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,306,256	0	T	C	100.0%	794.3 / NA	243	E127G (GAA→GGA)	yojI	putative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (146/97); total (146/97)

Only 100 of 254 total aligned reads displayed.

ATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCAATGCG  >  NC_000913/2306166‑2306344

aTCCAGATACCCGTCACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTccc                                                                                        <  2:3844909/93‑1 (MQ=255)

  ccAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCgg                                                                                      <  1:8682429/93‑1 (MQ=255)

    aGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCa                                                                                    >  2:2073709/1‑93 (MQ=255)

    aGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCa                                                                                    <  2:510140/93‑1 (MQ=255)

     gATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAg                                                                                   >  1:5923371/1‑93 (MQ=255)

     gATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAg                                                                                   >  1:170287/1‑93 (MQ=255)

     gATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAg                                                                                   >  1:7416251/1‑93 (MQ=255)

      aTAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGa                                                                                  >  1:1219894/1‑93 (MQ=255)

      aTAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGa                                                                                  <  2:2702072/93‑1 (MQ=255)

       tAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGAc                                                                                 <  1:2344561/93‑1 (MQ=255)

        aGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACg                                                                                >  2:2157751/1‑93 (MQ=255)

        aGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACg                                                                                >  1:9173152/1‑93 (MQ=255)

         gCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGc                                                                               >  2:7750611/1‑93 (MQ=255)

          ccGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGca                                                                              >  1:324203/1‑93 (MQ=255)

             ttcccAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCGCaa                                                                           <  2:8938200/90‑1 (MQ=255)

             ttACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACaa                                                                           >  2:6667461/1‑93 (MQ=255)

              tACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACaaa                                                                          >  2:3975400/1‑93 (MQ=255)

              tACCAGCAACATATTGCCCGACATCGTCCACAGATACGCCGCTGAGCCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACaaa                                                                          <  2:3454111/93‑1 (MQ=255)

               aCCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACaaaa                                                                         <  1:203920/93‑1 (MQ=255)

                ccAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAg                                                                        >  2:4446899/1‑93 (MQ=255)

                ccAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAg                                                                        <  2:4771897/93‑1 (MQ=255)

                  aGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCa                                                                      >  2:8343062/1‑93 (MQ=255)

                  aGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCa                                                                      <  1:6885690/93‑1 (MQ=255)

                   gCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCaa                                                                     >  1:7913071/1‑93 (MQ=255)

                    cAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAAt                                                                    <  1:9771359/93‑1 (MQ=255)

                     aaCATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATg                                                                   <  2:500509/93‑1 (MQ=255)

                      aCATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATgg                                                                  <  2:1667840/93‑1 (MQ=255)

                      aCATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATgg                                                                  <  1:9349896/93‑1 (MQ=255)

                       cATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGt                                                                 >  2:606134/1‑93 (MQ=255)

                         ttttGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGa                                                               >  2:4323266/1‑93 (MQ=255)

                         ttttGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGa                                                               >  1:8523960/1‑93 (MQ=255)

                         ttttGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGa                                                               >  1:3019063/1‑93 (MQ=255)

                          tttGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGat                                                              >  2:2753506/1‑93 (MQ=255)

                           ttGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGata                                                             <  1:281509/93‑1 (MQ=255)

                           ttGCCCGACAGCATCCACAGACACGCCGCTGAACCGATAGTGAGAATGAGCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGata                                                             <  2:3179783/93‑1 (MQ=255)

                            tGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGatat                                                            <  2:4847332/93‑1 (MQ=255)

                            tGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGatat                                                            <  1:9318750/93‑1 (MQ=255)

                             gCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATAtt                                                           <  2:237819/93‑1 (MQ=255)

                             gCCAGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATAtt                                                           <  2:9191378/93‑1 (MQ=255)

                                cGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTgcg                                                        >  1:3575816/1‑93 (MQ=255)

                                 gACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTgcgc                                                       >  2:3658304/1‑93 (MQ=255)

                                  aCAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGca                                                      >  2:6294977/1‑93 (MQ=255)

                                  aCAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGca                                                      >  1:5696361/1‑93 (MQ=255)

                                       aTCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCg                                                 <  1:3322902/93‑1 (MQ=255)

                                        tCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGc                                                <  2:2991584/93‑1 (MQ=255)

                                        tCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGc                                                >  2:3936191/1‑93 (MQ=255)

                                        tCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGc                                                >  2:2501198/1‑93 (MQ=255)

                                         ccACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCt                                               <  1:8183103/93‑1 (MQ=255)

                                            caGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGt                                            <  2:2211223/93‑1 (MQ=255)

                                            caGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGt                                            >  2:6899065/1‑93 (MQ=255)

                                            caGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGt                                            <  1:6834170/93‑1 (MQ=255)

                                              gATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTa                                          >  1:6306559/1‑93 (MQ=255)

                                              gATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTa                                          >  1:4016378/1‑93 (MQ=255)

                                               aTACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTaa                                         <  2:8210107/93‑1 (MQ=255)

                                               aTACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTaa                                         <  1:354059/93‑1 (MQ=255)

                                                tACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAc                                        >  2:1222110/1‑93 (MQ=255)

                                                tACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAc                                        >  2:1099838/1‑93 (MQ=255)

                                                tACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAc                                        <  2:7067593/93‑1 (MQ=255)

                                                tACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAc                                        <  1:5185153/93‑1 (MQ=255)

                                                tACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCAAATCGCTGGTTAAc                                        >  1:7289378/1‑93 (MQ=255)

                                                 aCGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAcc                                       <  2:1253475/93‑1 (MQ=255)

                                                 aCGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAcc                                       <  1:7209230/93‑1 (MQ=255)

                                                  cgccgcTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAAccc                                      <  2:3815517/93‑1 (MQ=255)

                                                    ccgcTGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCg                                    <  2:983982/93‑1 (MQ=255)

                                                      gcTGAACCGATAGTGAGAATGATCCCCCGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGcc                                  >  1:1936451/1‑93 (MQ=255)

                                                        tGAACCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAg                                <  2:1793873/93‑1 (MQ=255)

                                                           aCCGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCaa                             >  2:5638549/1‑93 (MQ=255)

                                                            ccGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAAc                            >  2:8844535/1‑93 (MQ=255)

                                                             cGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACg                           >  1:3499431/1‑93 (MQ=255)

                                                             cGATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACg                           <  1:8286867/93‑1 (MQ=255)

                                                              gATAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGa                          >  2:6052030/1‑93 (MQ=255)

                                                                tAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTGACCCCGCCAGCAACGAgg                        >  2:7610028/1‑93 (MQ=255)

                                                                tAGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAgg                        <  1:1105543/93‑1 (MQ=255)

                                                                aaGTGAGAATGATCCCCTGCAACAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAgg                        <  2:9505715/92‑1 (MQ=255)

                                                                 aGTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgc                       <  2:8108336/93‑1 (MQ=255)

                                                                  gTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgcg                      >  1:2589875/1‑93 (MQ=255)

                                                                  gTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgcg                      <  2:4759656/93‑1 (MQ=255)

                                                                  gTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgcg                      >  1:5109504/1‑93 (MQ=255)

                                                                  gTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgcg                      >  1:9820523/1‑93 (MQ=255)

                                                                  gTGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgcg                      >  2:693594/1‑93 (MQ=255)

                                                                   tGAGAATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGgcgc                     >  1:3680696/1‑93 (MQ=255)

                                                                      gaATGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTAc                  <  2:5307280/93‑1 (MQ=255)

                                                                        aTGATCCCCTGCCCCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCg                <  2:7990236/93‑1 (MQ=255)

                                                                        aTGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCg                >  1:1521665/1‑93 (MQ=255)

                                                                         tGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGa               <  1:4482864/93‑1 (MQ=255)

                                                                         tGATCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGa               >  1:1612196/1‑93 (MQ=255)

                                                                         tGACCCCCTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGa               <  2:2256527/93‑1 (MQ=255)

                                                                             ccccTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGCgttg           >  1:6952039/1‑93 (MQ=255)

                                                                             ccccTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAgttg           >  2:1432283/1‑93 (MQ=255)

                                                                             ccccTGCACCAGTCCCGGCAGACGCACAAAAGCAACGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAgttg           >  2:7838819/1‑93 (MQ=255)

                                                                               ccTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAgttgtt         <  1:4256602/93‑1 (MQ=255)

                                                                               ccTGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAgttgtt         >  1:6086738/1‑93 (MQ=255)

                                                                                 tGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCa       >  1:8472647/1‑93 (MQ=255)

                                                                                 tGCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCa       >  2:18241/1‑93 (MQ=255)

                                                                                  gCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCaa      <  2:5524048/93‑1 (MQ=255)

                                                                                  gCACCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCaa      <  1:147731/93‑1 (MQ=255)

                                                                                    aCCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCAATg    >  2:3101856/1‑93 (MQ=255)

                                                                                    aCCAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCAATg    <  2:4203863/93‑1 (MQ=255)

                                                                                      cAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCAATgcg  <  1:5990185/93‑1 (MQ=255)

                                                                                      cAGTCCCGGCAGACGCACAAAAGCAATGGTGATATTGAGCACATCGCTGGTTAACCCCGCCAGCAACAAGGTGATACTGAGCTGTTCAAtcgg  >  2:680648/1‑90 (MQ=255)

ATCCAGATAGCCGTTACCAGCAACATTTTGCCCGACAGCATCCACAGATACGCCGCTGAACCGATAGTGAGAATGATCCCCTGCACCAGTTCCGGCAGACGCACAAAAGCAATGGTGATATTGCGCACATCGCTGGTTAACCCCGCCAGCAACGAGGCGCTACCGAGTTGTTCAATGCG  >  NC_000913/2306166‑2306344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`