BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,682	T→C	A324A (GCA→GCG)	b2249 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,682	0	T	C	99.3%	437.7 / ‑6.4	142	A324A (GCA→GCG)	b2249	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (97/44); total (97/45)
Fisher's exact test for biased strand distribution p-value = 3.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 210 total aligned reads displayed.

AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACA  >  NC_000913/2360592‑2360771

aGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAgcgc                                                                                         >  2:4389643/1‑93 (MQ=255)

aGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAgcgc                                                                                         <  2:4990575/93‑1 (MQ=255)

 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAACGTTGAGATGCTCGTTCTCGAAACCCGAAACGGCCAGCGcc                                                                                        >  1:8184893/1‑93 (MQ=255)

 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGcc                                                                                        >  1:5920545/1‑93 (MQ=255)

 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGcc                                                                                        >  2:4351052/1‑93 (MQ=255)

 gTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGcc                                                                                        >  2:7055595/1‑93 (MQ=255)

  tGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCa                                                                                       >  1:6859942/1‑93 (MQ=255)

  tGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCa                                                                                       >  1:9706113/1‑93 (MQ=255)

   gCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAg                                                                                      <  1:1334406/93‑1 (MQ=255)

   gCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAg                                                                                      >  2:9608295/1‑93 (MQ=255)

   gCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAg                                                                                      >  2:1516168/1‑93 (MQ=255)

    cTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAGCCGGAAACAgccagcgccagc                                                                                     <  2:1627581/93‑1 (MQ=255)

    cTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAgccagcgccagc                                                                                     <  2:3400600/93‑1 (MQ=255)

     tGAAACGCGCACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGcc                                                                                    <  2:3876858/93‑1 (MQ=255)

      gAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGccc                                                                                   <  2:3646844/93‑1 (MQ=255)

      gAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGccc                                                                                   >  1:9550831/1‑93 (MQ=255)

       aaaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCg                                                                                  <  1:7740882/93‑1 (MQ=255)

        aaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCACCGCCAGCCCgg                                                                                 >  2:2791998/1‑93 (MQ=255)

        aaCGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTCGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCgg                                                                                 <  2:2484887/93‑1 (MQ=255)

          cGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCa                                                                               <  1:3023914/93‑1 (MQ=255)

            cacaCGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaa                                                                             >  2:7743349/1‑93 (MQ=255)

             acacGCAGAGCGAAATTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaaa                                                                            <  1:1828449/93‑1 (MQ=255)

             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCaaaa                                                                            <  2:4019399/93‑1 (MQ=255)

             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaaa                                                                            <  1:9121891/93‑1 (MQ=255)

             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCaaaa                                                                            >  1:2608365/1‑93 (MQ=255)

             acacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCACCCCGGCaaaa                                                                            >  1:7829162/1‑93 (MQ=255)

              cacGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAAt                                                                           <  2:3115502/93‑1 (MQ=255)

                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGtt                                                                       <  2:3285232/93‑1 (MQ=255)

                  cAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGtt                                                                       <  2:6520901/93‑1 (MQ=255)

                   agagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTg                                                                      >  1:5473505/1‑93 (MQ=255)

                   agagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTg                                                                      >  1:4597244/1‑93 (MQ=255)

                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTgg                                                                     >  1:4665707/1‑93 (MQ=255)

                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTgg                                                                     >  2:9067769/1‑93 (MQ=255)

                    gagCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTgg                                                                     >  1:2120805/1‑93 (MQ=255)

                      gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGcc                                                                   <  1:5793515/93‑1 (MQ=255)

                      gCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGcc                                                                   >  2:2722991/1‑93 (MQ=255)

                       cGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGccc                                                                  >  1:9369219/1‑93 (MQ=255)

                       cGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGACGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGccc                                                                  <  2:9234007/93‑1 (MQ=255)

                          aaGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCccgcc                                                               >  1:2231707/1‑93 (MQ=255)

                            gTGCGGTCTGGCGGGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGt                                                             <  2:7881171/93‑1 (MQ=255)

                            gTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGt                                                             <  2:5943085/93‑1 (MQ=255)

                            gTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGt                                                             >  2:8658060/1‑93 (MQ=255)

                             tGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGtt                                                            >  1:8710986/1‑93 (MQ=255)

                             ggCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGtt                                                            <  1:7621888/92‑1 (MQ=255)

                              gCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTc                                                           >  2:6119062/1‑93 (MQ=255)

                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGt                                                        >  1:8878754/1‑93 (MQ=255)

                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGt                                                        >  1:7647403/1‑93 (MQ=255)

                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGt                                                        >  1:5289429/1‑93 (MQ=255)

                                 gTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGt                                                        <  1:4247878/93‑1 (MQ=255)

                                   cgggCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTaa                                                      <  1:1783167/91‑1 (MQ=255)

                                   cTGGCGTGGCTAGCGCAAAGTTGATATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTaa                                                      <  1:7933675/93‑1 (MQ=255)

                                    tGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAAt                                                     >  2:1432472/1‑93 (MQ=255)

                                     ggCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATc                                                    <  1:4447647/93‑1 (MQ=255)

                                      gCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATcc                                                   >  2:5663951/1‑93 (MQ=255)

                                      gCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATcc                                                   >  2:10006814/1‑93 (MQ=255)

                                       cGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCa                                                  <  2:814456/93‑1 (MQ=255)

                                         tGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                >  2:471789/1‑93 (MQ=255)

                                         tGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGt                                                >  2:7998761/1‑93 (MQ=255)

                                          ggCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTg                                               >  2:2193340/1‑93 (MQ=255)

                                          ggCTAGCCCAAAGTGGAGCTCCTCGTTCGCAAACCCCGAAACAGCCGGCGCTACCCCGGCAAATTGGTTGCCCCGCCGTTCTGTAATCCAGTg                                               <  2:5209919/93‑1 (MQ=255)

                                            cgaGCGCAAAGTGGAGATGCTCGTCCGCGAAACCCGAAACAGCCGGCACATGCCCGGTAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg                                             <  2:6221285/91‑1 (MQ=255)

                                            cTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTgcg                                             >  2:10036354/1‑93 (MQ=255)

                                               gcgcAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAg                                          >  2:8876520/1‑93 (MQ=255)

                                                  cAAAGTGGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGttt                                       <  1:4130210/93‑1 (MQ=255)

                                                    aggTTGAGATGCTCGTTCTCGAACCCGAAAACAGCCAGCGCCAGCCCGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTgc                                     <  2:7480085/91‑1 (MQ=255)

                                                        tGAGATGCTCGTTCTCGAACCCCGAAACACCCAGCCCCAGCCCGGCAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCa                                 <  1:6880333/93‑1 (MQ=255)

                                                         gagtTGCTGGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg                                <  2:2578576/93‑1 (MQ=255)

                                                         gagaTGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg                                >  2:3092977/1‑93 (MQ=255)

                                                         gagaTGCTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAg                                >  2:4593312/1‑93 (MQ=255)

                                                            aTGCTCGTTCTCGAACCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGt                             <  1:6996942/93‑1 (MQ=255)

                                                              tctctTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGttt                           <  2:2853367/92‑1 (MQ=255)

                                                               cTCGTTCTCGAAGCCCGAAACAGACAGCGCCAGCCCGGCAAAATGGTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtc                          <  2:6928788/93‑1 (MQ=255)

                                                               cTCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtc                          >  1:1933070/1‑93 (MQ=255)

                                                                tCGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcc                         >  1:2395398/1‑93 (MQ=255)

                                                                 cGTTCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcct                        >  1:9756291/1‑93 (MQ=255)

                                                                   ttCTCGAAACCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTtcctcc                      >  1:862067/1‑93 (MQ=255)

                                                                    tctcAAACCCTGAATCAGCCGGCGCCAGCCGGCGAAAATGTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTcctcct                     <  1:5151438/93‑1 (MQ=255)

                                                                      acGAAACCCAAAACAGCCCGCGCCGGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgt                   <  1:7489956/92‑1 (MQ=255)

                                                                       cGAACCCGGAACCAGCCAGCGCCAGCCGGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCtgtg                  <  2:1313831/93‑1 (MQ=255)

                                                                         aaaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa                >  2:7146818/1‑93 (MQ=255)

                                                                         aaaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGaa                >  1:6094791/1‑93 (MQ=255)

                                                                          aaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAg               <  2:5392428/93‑1 (MQ=255)

                                                                          aaCCCGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAg               >  1:10001565/1‑93 (MQ=255)

                                                                           aCCCAAACCAGCCAGCGCCGGCCGGGCAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAgg              <  2:5621396/93‑1 (MQ=255)

                                                                             ccaaaaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGaa            <  1:7521396/90‑1 (MQ=255)

                                                                             ccGAAACAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGaa            >  2:1323230/1‑93 (MQ=255)

                                                                              caaaaCAGCCAGCGCCACCCCGGCAAAAGGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           <  2:4971495/91‑1 (MQ=255)

                                                                              caaaaCAGCCACCGCCAGCCCGGCAAAAGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           <  2:9209493/91‑1 (MQ=255)

                                                                              cGACACAGCCAGCGCCAGCCGGGAAAAATGGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAc           <  2:8589864/93‑1 (MQ=255)

                                                                               caaaCACCCGGCGCCAGCCCGGCAAAAGGGTGGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAcc          <  2:1401690/92‑1 (MQ=255)

                                                                                aaaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAccg         >  2:9882325/1‑92 (MQ=255)

                                                                                 aaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAccgc        >  1:4384330/1‑91 (MQ=255)

                                                                                 aaCAGCCAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAAccgc        >  1:3297380/1‑91 (MQ=255)

                                                                                     gccagcgccagcCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca    >  1:6462305/1‑93 (MQ=255)

                                                                                     gccagcgccagcCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca    >  1:8294114/1‑93 (MQ=255)

                                                                                      ccAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcac   >  2:1844968/1‑93 (MQ=255)

                                                                                      ccAGCGCCACCACGACAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcac   <  1:5393827/93‑1 (MQ=255)

                                                                                       cAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca  <  1:3081330/93‑1 (MQ=255)

                                                                                       cAGCGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca  >  1:1375087/1‑93 (MQ=255)

                                                                                       cAGCGCCAGCCCGGAAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca  <  1:6570823/93‑1 (MQ=255)

AGTGCTGAAACGCACACGCAGAGCGAAAGTGCCGTCTGGCGTGGCTAGCGCAAAGTTGAGATGCTCGTTCTCGAAACCCGAAACAGCCAGTGCCAGCCCGGCAAAATGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACA  >  NC_000913/2360592‑2360771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`