BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,360,764	A→G	V297A (GTG→GCG)	b2249 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,360,764	0	A	G	95.9%	718.0 / 10.8	242	V297A (GTG→GCG)	b2249	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (3/7); new base G (114/118); total (117/125)
Fisher's exact test for biased strand distribution p-value = 3.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 250 total aligned reads displayed.

GCCAGTGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG  >  NC_000913/2360677‑2360855

gccagcgccagcCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca                                                                                        >  1:8294114/1‑93 (MQ=255)

gccagcgccagcCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTca                                                                                        >  2:1173400/1‑93 (MQ=255)

  cAGCGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca                                                                                      >  1:5392774/1‑93 (MQ=255)

  cAGCGCCACCCCGGAAAAAT‑GTTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca                                                                                      <  1:3742680/93‑1 (MQ=255)

  cAGCGCCACCCCGGAAAAAT‑GGTCGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTcaca                                                                                      <  1:494469/93‑1 (MQ=255)

    gcgCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACg                                                                                    >  1:3166630/3‑93 (MQ=255)

    gcgCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACg                                                                                    >  1:5744348/3‑93 (MQ=255)

     cgCCACCCGGGAAAAAT‑TTTCGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGc                                                                                   <  2:9571684/92‑1 (MQ=255)

      gCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGCACCGATTCACACgac                                                                                  >  2:5314269/1‑91 (MQ=255)

         cgCCGGGAAAAAT‑GTTGGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGc                                                                               <  2:714255/92‑1 (MQ=255)

         aGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGc                                                                               <  1:8021330/93‑1 (MQ=255)

           cccGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCaa                                                                             >  1:9435902/1‑93 (MQ=255)

            ccGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAAt                                                                            >  2:1031960/1‑93 (MQ=255)

              gggaaaaTGGGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATg                                                                           <  1:17232/90‑1 (MQ=255)

              ggaaaaaT‑GTTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATgg                                                                          <  1:6577546/90‑1 (MQ=255)

               gCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGa                                                                         <  2:6191601/93‑1 (MQ=255)

                cAAAAT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAg                                                                        <  1:1690269/93‑1 (MQ=255)

                 aaaaT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCGATGGAGc                                                                       >  2:4223308/1‑93 (MQ=255)

                 aaaaT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAga                                                                       >  2:3665654/1‑92 (MQ=255)

                  aaaT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCa                                                                      >  1:6798576/1‑93 (MQ=255)

                   agt‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCAc                                                                     <  1:6550736/91‑1 (MQ=255)

                   aaT‑GGTTGCCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCAc                                                                     <  1:9530437/93‑1 (MQ=255)

                     t‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCt                                                                   <  1:1894913/93‑1 (MQ=255)

                     t‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCt                                                                   >  2:7503233/1‑93 (MQ=255)

                            ccccGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCga                                                             <  2:6929792/92‑1 (MQ=255)

                             cccGCCTTTCTGTAATCCATTGCGCAGGTTGCGCCAGTGTTTCCTCCTGTGGAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCgag                                                            <  1:6391153/93‑1 (MQ=255)

                             cccGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCgag                                                            >  2:6322592/1‑93 (MQ=255)

                               cgccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaa                                                          <  1:3477804/93‑1 (MQ=255)

                               cgccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaa                                                          >  2:609528/1‑93 (MQ=255)

                               cgccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaa                                                          <  2:4597243/93‑1 (MQ=255)

                                gccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGaaa                                                         >  2:5738252/1‑93 (MQ=255)

                                 ccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAg                                                        >  2:389437/1‑93 (MQ=255)

                                 ccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAg                                                        >  2:167206/1‑93 (MQ=255)

                                 ccgTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAg                                                        >  2:9134696/1‑93 (MQ=255)

                                   gTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGtt                                                      >  2:3447485/1‑93 (MQ=255)

                                    ttCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTg                                                     <  1:6834101/93‑1 (MQ=255)

                                    ttCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTg                                                     <  2:8493077/93‑1 (MQ=255)

                                    ttCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTg                                                     >  1:2149764/1‑93 (MQ=255)

                                     tCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGc                                                    <  2:3258953/93‑1 (MQ=255)

                                       tGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaa                                                  >  1:7918969/1‑93 (MQ=255)

                                       tGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaa                                                  >  2:526856/1‑93 (MQ=255)

                                       tGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaa                                                  <  2:2329823/93‑1 (MQ=255)

                                        gTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCaaa                                                 >  2:9039241/1‑93 (MQ=255)

                                         tAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACATGCGCGAGAAAGTTGCAAAg                                                <  2:562773/93‑1 (MQ=255)

                                           aTCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGcc                                              <  2:5473504/93‑1 (MQ=255)

                                           aTCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGcc                                              <  1:1022051/93‑1 (MQ=255)

                                            tCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCa                                             <  2:8724921/93‑1 (MQ=255)

                                            tCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCa                                             >  2:951947/1‑93 (MQ=255)

                                             cgaGTGCGCAGGTTGCGCCAGGGTTTCCTCCTGTGAAGTAACCGCTTCGCACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCaa                                            <  1:5122481/91‑1 (MQ=255)

                                             ccAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCaa                                            <  2:5928511/93‑1 (MQ=255)

                                                gTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaa                                         >  1:8847733/1‑93 (MQ=255)

                                                gTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaa                                         >  1:9661199/1‑93 (MQ=255)

                                                 tGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaaa                                        <  2:3985633/93‑1 (MQ=255)

                                                 tGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATaaa                                        >  1:7557565/1‑93 (MQ=255)

                                                  gcgcAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAac                                       <  2:6636333/93‑1 (MQ=255)

                                                   cgcAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAacc                                      <  2:9355785/93‑1 (MQ=255)

                                                   cgcAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAacc                                      >  1:3775683/1‑93 (MQ=255)

                                                     cAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAaccac                                    >  1:9288525/1‑93 (MQ=255)

                                                      aGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCCCCTGCGCGAGAAAGTTGCAAAGCCAATAAaccacc                                   <  1:2838822/93‑1 (MQ=255)

                                                       gTTTGCGCCAGGGTTTCCTCCTGTGAAGTCACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCg                                  <  1:2668709/93‑1 (MQ=255)

                                                       gTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCg                                  >  1:4344282/1‑93 (MQ=255)

                                                        tttGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCgg                                 >  2:7281573/1‑93 (MQ=255)

                                                         ttGCGCCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGt                                <  2:7021029/93‑1 (MQ=255)

                                                            cgcCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGaa                             <  2:3552249/93‑1 (MQ=255)

                                                             gcCAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAt                            <  2:6507586/93‑1 (MQ=255)

                                                              ccAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAtt                           <  2:8773886/93‑1 (MQ=255)

                                                              ccAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAAtt                           >  1:4552167/1‑93 (MQ=255)

                                                               cAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTg                          <  2:8330790/93‑1 (MQ=255)

                                                               cAGGGTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTg                          >  2:4710410/1‑93 (MQ=255)

                                                                 gggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgct                        <  2:626520/93‑1 (MQ=255)

                                                                 gggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgct                        >  2:7062479/1‑93 (MQ=255)

                                                                  ggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctc                       <  1:5865998/93‑1 (MQ=255)

                                                                  ggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctc                       >  2:2436786/1‑93 (MQ=255)

                                                                  ggTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctc                       <  2:1710238/93‑1 (MQ=255)

                                                                   gTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcg                      >  1:6213543/1‑93 (MQ=255)

                                                                   gTTTCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcg                      <  1:4406239/93‑1 (MQ=255)

                                                                    tttCCTCCTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcgc                     >  2:1581716/1‑93 (MQ=255)

                                                                      tcctccTGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTgctcgctc                   >  2:4783024/1‑93 (MQ=255)

                                                                       cctcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCa                  >  1:1504252/1‑93 (MQ=255)

                                                                        ctcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCTGTGAATTGCTCGCTCaa                 <  1:1242181/93‑1 (MQ=255)

                                                                        ctcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCaa                 >  1:9874301/1‑93 (MQ=255)

                                                                         tcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAAc                >  2:4415326/1‑93 (MQ=255)

                                                                         tcctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAAc                >  1:9085157/1‑93 (MQ=255)

                                                                          cctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACg               <  2:9369218/93‑1 (MQ=255)

                                                                          cctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACg               <  1:7743350/93‑1 (MQ=255)

                                                                           ctGTGAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGt              <  1:8557775/93‑1 (MQ=255)

                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             <  2:3194326/93‑1 (MQ=255)

                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             <  1:733139/93‑1 (MQ=255)

                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             >  1:8051675/1‑93 (MQ=255)

                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             >  2:1482095/1‑93 (MQ=255)

                                                                            tgtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTc             <  2:304640/93‑1 (MQ=255)

                                                                             gtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCa            >  1:918761/1‑93 (MQ=255)

                                                                             gtgAAGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCa            <  1:5661752/93‑1 (MQ=255)

                                                                                aaGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGc         <  2:2633624/93‑1 (MQ=255)

                                                                                aaGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGc         >  1:5958132/1‑93 (MQ=255)

                                                                                 aGGAACCGCTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCt        <  1:4621183/93‑1 (MQ=255)

                                                                                    aaCCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGaa     <  2:7726307/93‑1 (MQ=255)

                                                                                    aaCCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGaa     <  2:198517/93‑1 (MQ=255)

                                                                                     aCCGCTTCACACGCCAGCAATGGAGCAGCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAc    <  1:4825677/93‑1 (MQ=255)

                                                                                     aCCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAAc    <  1:3962552/93‑1 (MQ=255)

GCCAGTGCCAGCCCGGCAAAAT‑GGTTGGCCCGCCGTTCTGTAATCCAGTGCGCAGTTTGCGCCAGGGTTTCCTCCTGTGAAGGAACCACTTCACACGCCAGCAATGGAGCACCTGCGCGAGAAAGTTGCAAAGCCAATAAACCACCGGTGAATTGCTCGCTCAACGTCAGGCTGAACTG  >  NC_000913/2360677‑2360855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`