BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,362,769	G→A	E66K (GAA→AAA)	yfaO →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,362,769	0	G	A	99.0%	1502.8 / ‑1.9	415	E66K (GAA→AAA)	yfaO	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/4); new base A (205/203); total (208/207)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 431 total aligned reads displayed.

TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858

tCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACaaaa                                                                                         <  2:2268135/93‑1 (MQ=255)

tCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACaaaa                                                                                         >  2:5023590/1‑93 (MQ=255)

 cGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAaaaa                                                                                        <  2:6793766/93‑1 (MQ=255)

  gggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAAt                                                                                       <  2:7614003/93‑1 (MQ=255)

   ggTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATc                                                                                      <  2:6231680/93‑1 (MQ=255)

    gTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCa                                                                                     <  1:5298134/93‑1 (MQ=255)

    gTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCa                                                                                     <  2:8539892/93‑1 (MQ=255)

      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  2:2695748/93‑1 (MQ=255)

      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  2:9438745/93‑1 (MQ=255)

      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   <  1:1841040/93‑1 (MQ=255)

      ggcggcGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACg                                                                                   >  1:3708135/1‑93 (MQ=255)

       gcggcgTGGAGCCTGGCGAACGAATGGAAGAGGCACTCCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  2:5463472/93‑1 (MQ=255)

       gcggcgTGGAGCCTCGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGc                                                                                  <  1:2787108/93‑1 (MQ=255)

        cggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGcc                                                                                 >  2:2903287/1‑93 (MQ=255)

         ggcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCg                                                                                <  1:7428409/93‑1 (MQ=255)

          gcgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGt                                                                               <  1:8263852/93‑1 (MQ=255)

           cgTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTg                                                                              >  1:9018688/1‑93 (MQ=255)

            gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  1:10012310/1‑93 (MQ=255)

            gTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTgg                                                                             >  1:9921465/1‑93 (MQ=255)

             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            <  1:5078979/93‑1 (MQ=255)

             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  2:7955364/1‑93 (MQ=255)

             tGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGa                                                                            >  2:7222919/1‑93 (MQ=255)

              ggAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGAc                                                                           <  1:1195025/93‑1 (MQ=255)

                aGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCt                                                                         <  2:8895890/93‑1 (MQ=255)

                   cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACACAAATCACGCCGTGGACCTTCa                                                                      >  2:4561385/1‑93 (MQ=255)

                   cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      >  1:4710033/1‑93 (MQ=255)

                   cTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCa                                                                      >  2:2360865/1‑93 (MQ=255)

                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  1:1588033/1‑93 (MQ=255)

                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  1:4099394/1‑93 (MQ=255)

                    tGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAg                                                                     >  1:6232304/1‑93 (MQ=255)

                     ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  2:9662325/1‑93 (MQ=255)

                     ggCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGc                                                                    >  1:4938550/1‑93 (MQ=255)

                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   <  2:7343167/93‑1 (MQ=255)

                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   >  2:3908928/1‑93 (MQ=255)

                      gCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCg                                                                   >  1:9403915/1‑93 (MQ=255)

                       cgaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCga                                                                  >  1:6154500/1‑93 (MQ=255)

                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 <  2:1897349/93‑1 (MQ=255)

                        gaacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgat                                                                 >  2:8361835/1‑93 (MQ=255)

                         aacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatg                                                                >  2:1395093/1‑93 (MQ=255)

                         aacgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatg                                                                >  1:9188912/1‑93 (MQ=255)

                          acgaaTTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCgatga                                                               <  2:9778230/93‑1 (MQ=255)

                          acgaaTTGAAGAGGCACTACGCCGCGAAAGTCGTGAAGAACGGGGAGAACAGCTGCGTTTGACAAAAATCACGCCGTGGACCTTCAGCgatga                                                               <  1:1924615/93‑1 (MQ=255)

                               ttGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTc                                                          >  1:2046951/1‑93 (MQ=255)

                                tGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTca                                                         >  1:3109007/1‑92 (MQ=255)

                                tGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCg                                                         >  2:6980612/1‑93 (MQ=255)

                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        <  2:4743478/93‑1 (MQ=255)

                                 gAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGc                                                        <  2:2194073/93‑1 (MQ=255)

                                   agagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAc                                                      <  1:6606138/93‑1 (MQ=255)

                                    gagGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCAcc                                                     <  1:2546243/93‑1 (MQ=255)

                                      ggCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCaa                                                   <  2:772635/93‑1 (MQ=255)

                                       gCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAg                                                  <  1:4507294/93‑1 (MQ=255)

                                       gCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAg                                                  <  2:448355/93‑1 (MQ=255)

                                        cACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCACGa                                                 >  2:10011233/1‑93 (MQ=255)

                                         aCTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGAc                                                >  1:6101286/1‑93 (MQ=255)

                                           tACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGt                                              >  2:9910520/1‑93 (MQ=255)

                                            aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             <  2:7530724/93‑1 (MQ=255)

                                            aCGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTa                                             <  2:4916800/93‑1 (MQ=255)

                                             cgccgaGAAATTGGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTAt                                            <  2:6592807/93‑1 (MQ=255)

                                               ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          >  2:2049393/1‑93 (MQ=255)

                                               ccgcGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGc                                          <  1:2342747/93‑1 (MQ=255)

                                                   gAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGAt                                      >  2:6007958/1‑93 (MQ=255)

                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATg                                     <  2:1094107/93‑1 (MQ=255)

                                                    aaaTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTAGCACCAAGACGTATGCAGATg                                     >  1:719539/1‑93 (MQ=255)

                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   <  1:3391287/93‑1 (MQ=255)

                                                      aTTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGt                                   <  1:7956807/93‑1 (MQ=255)

                                                       ttCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTc                                  <  1:6989343/93‑1 (MQ=255)

                                                           cgAAGAACTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCaa                              <  1:6817264/93‑1 (MQ=255)

                                                              agaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGa                           <  1:1048703/93‑1 (MQ=255)

                                                               gaaCTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGAACCAAGACGTATGCAGATGGTCGCAAGGaa                          >  2:9168120/1‑93 (MQ=255)

                                                                  cTGGGAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGAATGCAGATGTCCGCAACGAagat                       >  1:2897240/1‑92 (MQ=255)

                                                                    gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     <  1:220389/93‑1 (MQ=255)

                                                                    gggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAt                     >  2:5671655/1‑93 (MQ=255)

                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  2:5278555/93‑1 (MQ=255)

                                                                     ggAGAACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGAtt                    <  2:3630571/93‑1 (MQ=255)

                                                                       agaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTta                  >  1:9314562/1‑93 (MQ=255)

                                                                       agaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTta                  <  1:9563784/93‑1 (MQ=255)

                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  2:2580605/1‑93 (MQ=255)

                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  1:3210655/1‑93 (MQ=255)

                                                                        gaACAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtat                 >  2:6422496/1‑93 (MQ=255)

                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                <  1:8155424/93‑1 (MQ=255)

                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                <  1:9866354/93‑1 (MQ=255)

                                                                         aaCAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTtata                >  2:3124422/1‑93 (MQ=255)

                                                                           cAGCTGCTTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATg              >  2:3199673/1‑93 (MQ=255)

                                                                             gctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAt            >  1:4688874/1‑93 (MQ=255)

                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  1:8582380/1‑93 (MQ=255)

                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  2:1216474/1‑93 (MQ=255)

                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           >  2:2605725/1‑93 (MQ=255)

                                                                              ctgctTTTGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGAtt           <  1:586690/93‑1 (MQ=255)

                                                                                  ttttGACAAAAATCACGCCTTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTAcc       <  1:9781321/93‑1 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      <  2:8209050/93‑1 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:4154419/1‑93 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:6893246/1‑93 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  2:3463013/1‑93 (MQ=255)

                                                                                   tttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCt      >  1:8668842/1‑93 (MQ=255)

                                                                                    ttGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCGCCAAGACGTATGCAGATGGTCGC‑AGGAAGAGATTTATATGATTTACCTGa    <  2:806728/93‑1 (MQ=255)

                                                                                     tGACAAAAATCGCGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGa    <  1:7725156/93‑1 (MQ=255)

                                                                                     tGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGa    >  1:6463274/1‑93 (MQ=255)

                                                                                     tGACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTACATGATTTACCTGa    >  1:5206486/1‑93 (MQ=255)

                                                                                      gACAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAt   >  1:8451091/1‑93 (MQ=255)

                                                                                       aCAAAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGAtt  >  1:560086/1‑93 (MQ=255)

TCGGGTGGCGGCGTGGAGCCTGGCGAACGAATTGAAGAGGCACTACGCCGCGAAATTCGCGAAGAACTGGGAGAACAGCTGCTTTTGACAGAAATCACGCCGTGGACCTTCAGCGATGATATTCGCACCAAGACGTATGCAGATGGTCGCAAGGAAGAGATTTATATGATTTACCTGATT  >  NC_000913/2362679‑2362858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`