BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	285,775	A→G	Y386C (TAC→TGC)	yagG →	putative permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	285,775	0	A	G	99.5%	616.9 / ‑4.9	198	Y386C (TAC→TGC)	yagG	putative permease
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (96/101); total (96/102)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.82e-01

Only 100 of 211 total aligned reads displayed.

GCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCT  >  NC_000913/285685‑285864

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         <  2:10013002/93‑1 (MQ=255)

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         <  2:7588704/93‑1 (MQ=255)

gccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCg                                                                                         >  2:7736562/1‑93 (MQ=255)

 ccTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGgcgtgcgt                                                                                        <  1:4079587/93‑1 (MQ=255)

       cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                  <  1:327032/93‑1 (MQ=255)

       cGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTa                                                                                  <  1:2740492/93‑1 (MQ=255)

         ggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTAtt                                                                                >  1:9043754/1‑93 (MQ=255)

         ggCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTAtt                                                                                >  2:9710479/1‑93 (MQ=255)

          gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTc                                                                               <  2:3379665/93‑1 (MQ=255)

          gCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTc                                                                               <  2:7283322/93‑1 (MQ=255)

           cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTcc                                                                              >  1:5501354/1‑93 (MQ=255)

           cTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTcc                                                                              >  1:5998301/1‑93 (MQ=255)

            tGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCg                                                                             <  2:4815624/93‑1 (MQ=255)

              gtgtTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGGTCCTGGCGTGCGTCAACTGTTCCGcc                                                                           >  2:8149009/1‑93 (MQ=255)

                 ttCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagc                                                                        >  1:3951994/1‑93 (MQ=255)

                   ctcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCcagcag                                                                      >  2:418520/1‑93 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     >  2:1241012/1‑93 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     >  2:5957474/1‑93 (MQ=255)

                    tcCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagc                                                                     >  2:2211934/1‑93 (MQ=255)

                     ccACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagca                                                                    <  1:5470485/93‑1 (MQ=255)

                      cACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagcag                                                                   >  1:2342874/1‑93 (MQ=255)

                      cACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCcagcagcag                                                                   <  1:5357358/93‑1 (MQ=255)

                       acctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCagcagcagc                                                                  >  1:9738928/1‑93 (MQ=255)

                        cctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCg                                                                 <  2:6643680/93‑1 (MQ=255)

                        cctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCg                                                                 <  2:3449669/93‑1 (MQ=255)

                         ctacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGt                                                                >  1:5784339/1‑93 (MQ=255)

                          tacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTg                                                               >  1:7160463/1‑93 (MQ=255)

                          tacctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTg                                                               >  2:8690327/1‑93 (MQ=255)

                          tacctGTTCAGCCTGAAGATTGACCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTg                                                               >  2:6406102/1‑93 (MQ=255)

                           accgGTTCAGCCTGAACATTGGCCTTGCGATTGGCGGGGCGGTGGTTGGTCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGc                                                              <  1:5338932/93‑1 (MQ=255)

                            cctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGcg                                                             >  1:3713794/1‑92 (MQ=255)

                             ctGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAg                                                            >  1:6791212/1‑93 (MQ=255)

                              tGTTCAGCCTGAAGATTGGCCTGGCGATTGGCTGGGCGGTGGTGGTCTGGATCCTGGCGTTCGTCAACTATTCCGCCAGCAGCAGCGTGCAGc                                                           <  2:8731110/93‑1 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          >  2:9288851/1‑93 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          <  2:5487454/93‑1 (MQ=255)

                               gTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGcc                                                          >  1:4359998/1‑93 (MQ=255)

                                 tCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCgg                                                        >  2:436797/1‑93 (MQ=255)

                                 tCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCgg                                                        >  2:2882895/1‑93 (MQ=255)

                                     ccTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGa                                                    <  1:2816615/93‑1 (MQ=255)

                                     ccTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGa                                                    <  1:6143024/93‑1 (MQ=255)

                                      cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                   >  1:8624282/1‑93 (MQ=255)

                                      cTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAg                                                   <  2:956783/93‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  >  2:9453387/1‑93 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  <  2:1834430/93‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAgg                                                  <  1:5427806/93‑1 (MQ=255)

                                       tGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCAGGTTGAgg                                                  >  1:1252066/1‑93 (MQ=255)

                                        gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGt                                                 <  2:6974253/93‑1 (MQ=255)

                                        gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGt                                                 <  2:2632973/93‑1 (MQ=255)

                                        gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGt                                                 <  2:7448201/93‑1 (MQ=255)

                                        gAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGt                                                 >  2:1287323/1‑93 (MQ=255)

                                         aaGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTg                                                <  1:566000/93‑1 (MQ=255)

                                         aaGAGTGCCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTg                                                <  2:8025805/93‑1 (MQ=255)

                                           gATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCt                                              <  1:9991210/93‑1 (MQ=255)

                                             ttGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTca                                            >  2:5398821/1‑93 (MQ=255)

                                             ttGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTca                                            <  2:8478272/93‑1 (MQ=255)

                                                gCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTcacca                                         <  1:4883643/93‑1 (MQ=255)

                                                  cTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCaccacc                                       >  1:8730032/1‑93 (MQ=255)

                                                  cTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCaccacc                                       <  1:1662745/93‑1 (MQ=255)

                                                   tGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCAccacca                                      <  1:9346430/93‑1 (MQ=255)

                                                    ggCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCAt                                     <  2:2754903/93‑1 (MQ=255)

                                                      cGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCa                                   <  1:1517130/93‑1 (MQ=255)

                                                        aTTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCaaa                                 >  2:5830228/1‑93 (MQ=255)

                                                          tGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAt                               <  2:6810180/93‑1 (MQ=255)

                                                            gcggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttc                             <  1:1161977/93‑1 (MQ=255)

                                                            gcggggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttc                             <  1:9518776/93‑1 (MQ=255)

                                                            gcggggcggTGGTGGGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttc                             >  1:2134894/1‑93 (MQ=255)

                                                               ggtcggTGGTGGGCTGGATCCTGGCGTGCGACAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgt                          <  2:3588957/90‑1 (MQ=255)

                                                               gggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgt                          >  1:9883816/1‑93 (MQ=255)

                                                               gggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgt                          >  1:4222866/1‑93 (MQ=255)

                                                                ggcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgtt                         <  1:554861/93‑1 (MQ=255)

                                                                 gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                        >  1:1708468/1‑93 (MQ=255)

                                                                 gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                        <  2:4490656/93‑1 (MQ=255)

                                                                 gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                        >  2:7040475/1‑93 (MQ=255)

                                                                 gcggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                        <  1:9080832/93‑1 (MQ=255)

                                                                 gcggTGGTGCGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttc                        <  1:8842112/93‑1 (MQ=255)

                                                                  cggTGGTGGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAAttctgttct                       <  1:8502826/93‑1 (MQ=255)

                                                                    gtggtgGGCTGGCTCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCTTCAAAATTCTGTTCTGc                     <  2:8986062/93‑1 (MQ=255)

                                                                    gtggtgGGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGc                     >  2:6186219/1‑93 (MQ=255)

                                                                     tggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCg                    >  2:5268619/1‑93 (MQ=255)

                                                                     tggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCg                    <  1:769438/93‑1 (MQ=255)

                                                                      ggtggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgt                   >  1:10019988/1‑93 (MQ=255)

                                                                        tggGCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtgg                 >  1:7349345/1‑93 (MQ=255)

                                                                        tggGCCGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtgg                 <  1:3433682/93‑1 (MQ=255)

                                                                         ggtcTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtggt                <  2:4659812/90‑1 (MQ=255)

                                                                          ggCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtggtg               <  1:7301911/93‑1 (MQ=255)

                                                                          ggCTGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCgtggtg               >  1:6903624/1‑93 (MQ=255)

                                                                             tGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg            >  1:8313527/1‑93 (MQ=255)

                                                                             tGGATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCg            <  2:3723703/93‑1 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           >  2:1505488/1‑93 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           >  1:3381518/1‑93 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCgg           >  1:6646695/1‑93 (MQ=255)

                                                                              ggATCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTAACCACACTCAAAATTCTGTTCTGCGTGGTGCCgg           >  1:8740045/1‑93 (MQ=255)

                                                                                 tCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtgg        <  1:4764868/93‑1 (MQ=255)

                                                                                 tCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtgg        <  2:5371069/93‑1 (MQ=255)

                                                                                 tCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtgg        >  2:9215293/1‑93 (MQ=255)

                                                                                 tCCTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCggtgg        >  2:3047820/1‑93 (MQ=255)

                                                                                   cTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGgtggtg      >  1:408960/1‑93 (MQ=255)

                                                                                   cTGGCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGgtggtg      >  1:9686193/1‑93 (MQ=255)

                                                                                     ggCGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGct    >  1:6369660/1‑93 (MQ=255)

                                                                                       cGTGCGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGctct  <  1:5288492/93‑1 (MQ=255)

GCCTCGACGGGCTGGTGTTCTCCACCTACCTGTTCAGCCTGAAGATTGGCCTGGCGATTGGCGGGGCGGTGGTGGGCTGGATCCTGGCGTACGTCAACTATTCCGCCAGCAGCAGCGTGCAGCCGGTTGAGGTGCTCACCACCATCAAAATTCTGTTCTGCGTGGTGCCGGTGGTGCTCT  >  NC_000913/285685‑285864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`