BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,074	A→G	N145S (AAC→AGC)	yfcG →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,074	0	A	G	99.6%	848.5 / ‑5.3	264	N145S (AAC→AGC)	yfcG	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (129/134); total (129/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 273 total aligned reads displayed.

ATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTC  >  NC_000913/2418984‑2419163

catcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCa                                                                                         >  2:1104991‑M2/22‑93 (MQ=255)

 atcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCaa                                                                                        >  1:1865882‑M2/21‑93 (MQ=255)

    attttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCg                                                                                     <  1:7747573‑M2/76‑1 (MQ=255)

    attttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCg                                                                                     <  1:5825637‑M2/76‑1 (MQ=255)

        taatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGTGGCTg                                                                                 <  1:8028643‑M2/80‑1 (MQ=255)

         aatcacgccgccCCCCCCAACCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                <  2:1774104‑M2/81‑1 (MQ=255)

         aatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                <  1:4917795‑M2/81‑1 (MQ=255)

         aatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                >  1:2157497‑M2/13‑93 (MQ=255)

         aatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                <  1:6549344‑M2/81‑1 (MQ=255)

               cagcccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACt                                                                          <  1:1621263‑M2/87‑1 (MQ=255)

                cagccCCCCCCAAACCATACCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTc                                                                         <  2:537074‑M2/88‑1 (MQ=255)

                   ccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGcc                                                                      <  2:2305661‑M2/91‑1 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCct                                                                    >  2:5611920/1‑93 (MQ=255)

                      cccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctg                                                                   >  2:775483/1‑93 (MQ=255)

                      cccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctg                                                                   >  2:8190733/1‑93 (MQ=255)

                       ccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctgg                                                                  <  2:8868304/93‑1 (MQ=255)

                          cAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggctg                                                               <  1:8811397/93‑1 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggctgg                                                              >  1:5431011/1‑93 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCctggctgg                                                              <  1:1108128/93‑1 (MQ=255)

                            aaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTggg                                                             >  2:7110538/1‑93 (MQ=255)

                             aCCATTGCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGa                                                            <  1:4401059/93‑1 (MQ=255)

                             aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGa                                                            <  1:5964952/93‑1 (MQ=255)

                              acATTCCTTACGCTATTGATCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAg                                                           <  1:9355367/92‑1 (MQ=255)

                                aTTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGc                                                         >  1:6287958/1‑93 (MQ=255)

                                  tCCTTACGCTATTGAACGTTATCAGGTTGAAATTCAGCGTCTTTATCATGTACTGAGCTAGCGGCTGTAAAACTCCCCCTGGCTTGGAGgaga                                                       >  1:9015168/1‑93 (MQ=255)

                                  tCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCga                                                       <  2:3435860/93‑1 (MQ=255)

                                    cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCgaga                                                     >  1:8753026/1‑93 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCATCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGaa                                                    <  1:9680765/93‑1 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGaa                                                    <  1:4342024/93‑1 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGaa                                                    >  1:7050633/1‑93 (MQ=255)

                                     ttACGATATTGATCGTACTCAGGTTGAAACTCAGCGTCTTTACAATGAACTGAGCAAGCGGCTGGAAAACTCGCCCAGGCTGGGAGGAAAGaa                                                    <  2:8978473/93‑1 (MQ=255)

                                      tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAAc                                                   <  2:5312170/93‑1 (MQ=255)

                                        cGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTa                                                 <  2:7903005/93‑1 (MQ=255)

                                          cTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACa                                               <  1:733008/93‑1 (MQ=255)

                                          cTATTGAACGTGATCAGGTTGCAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACa                                               <  1:5067249/93‑1 (MQ=255)

                                            aTTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGc                                             >  1:393162/1‑93 (MQ=255)

                                              tGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAt                                           <  1:9883833/93‑1 (MQ=255)

                                              tGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAt                                           <  2:946090/93‑1 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          >  1:2584877/1‑93 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGCCGAGAACTACAGCAtt                                          >  2:1510138/1‑93 (MQ=255)

                                                aaCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGTAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTg                                         <  1:9676643/93‑1 (MQ=255)

                                                aaCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTg                                         >  2:1869158/1‑93 (MQ=255)

                                                 aCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGc                                        >  1:6431389/1‑93 (MQ=255)

                                                   gTTATTGGGTTGAAACTCAGCGTCTTTACCAGTTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCgg                                      <  1:476987/93‑1 (MQ=255)

                                                    ttATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGa                                     >  2:1652661/1‑93 (MQ=255)

                                                    ttATAAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGa                                     <  1:1190723/93‑1 (MQ=255)

                                                     tATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGat                                    <  2:5987752/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   <  2:2990438/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   <  2:5878742/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   >  2:9689534/1‑93 (MQ=255)

                                                        cAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATAtt                                 >  1:7550441/1‑93 (MQ=255)

                                                         aGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTg                                >  2:9408626/1‑93 (MQ=255)

                                                         aGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTg                                <  2:2961780/93‑1 (MQ=255)

                                                           gTTTAAACTCAGCGTCTTTACCATGGACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              <  1:9758367/93‑1 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              >  1:3873389/1‑93 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              <  2:7312889/93‑1 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              <  2:111465/93‑1 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              >  2:860229/1‑93 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  2:2580950/93‑1 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  2:6686991/93‑1 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  1:6149662/93‑1 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  2:1321175/93‑1 (MQ=255)

                                                            gtGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  1:5330742/92‑1 (MQ=255)

                                                              gAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                           <  2:5491897/93‑1 (MQ=255)

                                                               aaaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgct                          <  1:5701586/93‑1 (MQ=255)

                                                               aaaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgct                          >  2:4466702/1‑93 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         <  2:5014748/93‑1 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         >  2:4137449/1‑93 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         <  1:955761/93‑1 (MQ=255)

                                                                 aCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTgg                        >  1:135255/1‑93 (MQ=255)

                                                                 aCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTgg                        >  2:7493676/1‑93 (MQ=255)

                                                                    cAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCg                     >  1:8158797/1‑93 (MQ=255)

                                                                    cAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCg                     >  2:3330600/1‑93 (MQ=255)

                                                                     aGCGTCTTTACCATGTACTGAGCCAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGt                    <  1:2746050/93‑1 (MQ=255)

                                                                     aGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGt                    <  1:5007070/93‑1 (MQ=255)

                                                                       cGTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                  >  1:2071916/1‑93 (MQ=255)

                                                                        gTCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTggg                 <  1:8340555/93‑1 (MQ=255)

                                                                         tCTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGt                >  1:842384/1‑93 (MQ=255)

                                                                          cTTTACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGtt               >  1:3424908/1‑93 (MQ=255)

                                                                           tttACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTa              <  1:6704371/93‑1 (MQ=255)

                                                                            tgaCCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTTCCTGCTGGCCGTGGGTTaa             <  2:476051/91‑1 (MQ=255)

                                                                             tACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAt            <  2:4370025/93‑1 (MQ=255)

                                                                             tACCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAt            <  2:1576173/93‑1 (MQ=255)

                                                                              aCCATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATg           >  1:9165792/1‑93 (MQ=255)

                                                                              aCCATGTACTGAGCAAGCGGCTGGAAAACACGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAtc           >  1:2329082/1‑92 (MQ=255)

                                                                                cATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATggc         >  1:145525/1‑91 (MQ=255)

                                                                                cATGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc         >  1:4489500/1‑93 (MQ=255)

                                                                                 aTGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt        <  1:4132711/93‑1 (MQ=255)

                                                                                 aTGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt        >  2:7081177/1‑93 (MQ=255)

                                                                                  tGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTg       >  2:5834617/1‑93 (MQ=255)

                                                                                  tGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTg       >  2:872479/1‑93 (MQ=255)

                                                                                  tGTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTg       <  1:8567848/93‑1 (MQ=255)

                                                                                  tGTACGGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTg       <  1:465600/93‑1 (MQ=255)

                                                                                   gTACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTgg      <  1:6929587/93‑1 (MQ=255)

                                                                                    tACTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGa     <  2:9663991/93‑1 (MQ=255)

                                                                                      cTGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACt   >  1:3217743/1‑93 (MQ=255)

                                                                                       tGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATTCCTGGACTc  >  2:141702/1‑93 (MQ=255)

                                                                                       tGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTc  >  2:3773978/1‑93 (MQ=255)

                                                                                       tGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTc  <  2:3921888/93‑1 (MQ=255)

                                                                                       tGAGCAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTc  <  1:9461350/93‑1 (MQ=255)

ATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGAAAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTC  >  NC_000913/2418984‑2419163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`