BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	294,990	T→C	H444R (CAC→CGC)	intF ←	putative phage integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	294,990	0	T	C	99.5%	1968.9 / ‑3.2	609	H444R (CAC→CGC)	intF	putative phage integrase
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (312/294); total (314/295)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 638 total aligned reads displayed.

TATGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGTGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGCAACAATA  >  NC_000913/294900‑295079

tatGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCgcgc                                                                                         >  1:5133920/1‑93 (MQ=255)

tatGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCgcgc                                                                                         >  2:500154/1‑93 (MQ=255)

tacgTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCgcgc                                                                                         >  1:5999711/4‑93 (MQ=255)

  tGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCa                                                                                       >  2:2147988/1‑93 (MQ=255)

  tGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCa                                                                                       <  1:2212352/93‑1 (MQ=255)

   gTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAt                                                                                      >  2:8571528/1‑93 (MQ=255)

    ttGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAtt                                                                                     <  2:1188059/93‑1 (MQ=255)

    ttGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAtt                                                                                     >  2:5882484/1‑93 (MQ=255)

           ttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTaa                                                                              >  1:9705749/1‑93 (MQ=255)

            tAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAAc                                                                             >  2:775569/1‑93 (MQ=255)

            tAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAAc                                                                             <  1:3865839/93‑1 (MQ=255)

             aaTGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACa                                                                            >  1:2961429/1‑93 (MQ=255)

             aaTGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACa                                                                            <  1:5520456/93‑1 (MQ=255)

             aaTGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACa                                                                            >  2:6477750/1‑93 (MQ=255)

              aTGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAg                                                                           <  1:6290237/93‑1 (MQ=255)

               tGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGa                                                                          >  2:6984944/1‑93 (MQ=255)

                ggAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGAt                                                                         >  1:7070354/1‑93 (MQ=255)

                ggAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGAt                                                                         <  2:1403466/93‑1 (MQ=255)

                 gAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATc                                                                        >  2:9902274/1‑93 (MQ=255)

                   aTCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTCACAGATCTa                                                                      >  2:1606420/1‑93 (MQ=255)

                    tCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTaa                                                                     >  1:8809899/1‑93 (MQ=255)

                       aCGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACgg                                                                  >  2:8680818/1‑93 (MQ=255)

                       aCGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACgg                                                                  >  2:9383431/1‑93 (MQ=255)

                        cGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGga                                                                 >  2:196647/1‑93 (MQ=255)

                        cGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGga                                                                 <  2:6712853/93‑1 (MQ=255)

                         gCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgac                                                                >  2:7156029/1‑93 (MQ=255)

                           atatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacga                                                              <  2:7403168/93‑1 (MQ=255)

                           atatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacga                                                              <  2:3903673/93‑1 (MQ=255)

                            tatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacgac                                                             >  1:4854231/1‑93 (MQ=255)

                            tatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacgac                                                             >  1:6492158/1‑93 (MQ=255)

                            tatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacgac                                                             >  2:5273327/1‑93 (MQ=255)

                            tatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacgac                                                             <  1:3386648/93‑1 (MQ=255)

                             atCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGacgacg                                                            <  2:343507/93‑1 (MQ=255)

                               cAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGc                                                          <  2:6176681/93‑1 (MQ=255)

                                aaCGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCg                                                         <  1:3185543/93‑1 (MQ=255)

                                 aCGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGa                                                        <  1:3048034/93‑1 (MQ=255)

                                  cGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGAt                                                       <  2:6359344/93‑1 (MQ=255)

                                   gttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATa                                                      >  1:6821371/1‑93 (MQ=255)

                                    ttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAg                                                     <  1:7716581/93‑1 (MQ=255)

                                    ttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAg                                                     >  1:2096232/1‑93 (MQ=255)

                                      gttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAgtg                                                   >  1:2378072/1‑93 (MQ=255)

                                        tttttATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGtt                                                 <  1:7862618/93‑1 (MQ=255)

                                           ttATGGTACTACCTGCTTCATTTAAGATCCATGTCTCATTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGttttt                                              <  2:8941280/93‑1 (MQ=255)

                                             aTGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCg                                            >  1:4355472/1‑93 (MQ=255)

                                              tGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCgg                                           >  2:3059538/1‑93 (MQ=255)

                                                gTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCa                                         <  2:7594344/93‑1 (MQ=255)

                                                 tAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCaa                                        >  1:3335119/1‑93 (MQ=255)

                                                  aaTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAag                                       >  2:4687041/1‑93 (MQ=255)

                                                  aaTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAag                                       >  2:4092421/1‑93 (MQ=255)

                                                   aTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  2:3733876/93‑1 (MQ=255)

                                                   aTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  1:8272899/93‑1 (MQ=255)

                                                   aTACCTCCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAAGGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  2:2748302/93‑1 (MQ=255)

                                                    tACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAagag                                     <  1:9956508/93‑1 (MQ=255)

                                                    tACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAagag                                     <  1:5229976/93‑1 (MQ=255)

                                                    tACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAagag                                     >  1:4046595/1‑93 (MQ=255)

                                                    tACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAagag                                     >  1:8436105/1‑93 (MQ=255)

                                                     tccTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgc                                    <  1:738813/92‑1 (MQ=255)

                                                     tccTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgc                                    <  2:320915/92‑1 (MQ=255)

                                                     aCCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgc                                    <  2:951974/93‑1 (MQ=255)

                                                     aCCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgc                                    >  2:5588435/1‑93 (MQ=255)

                                                       cTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgcgc                                  >  2:9875405/1‑93 (MQ=255)

                                                       cTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgcgc                                  <  1:3699710/93‑1 (MQ=255)

                                                       cTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgcgc                                  >  1:8511571/1‑93 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                >  2:6565855/1‑93 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                >  2:2614247/1‑93 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                >  1:1921152/1‑93 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                >  1:1637233/1‑93 (MQ=255)

                                                          cTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTgg                               >  2:2982124/1‑93 (MQ=255)

                                                          cTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTgg                               >  1:6984527/1‑93 (MQ=255)

                                                           ttCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTggg                              >  2:8358931/1‑93 (MQ=255)

                                                            tCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGt                             >  1:7199877/1‑93 (MQ=255)

                                                            tCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGt                             >  1:9322053/1‑93 (MQ=255)

                                                             cATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGtt                            >  1:925741/1‑93 (MQ=255)

                                                             cATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGtt                            <  2:4407027/93‑1 (MQ=255)

                                                             cATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGtt                            >  1:7545896/1‑93 (MQ=255)

                                                              aTTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGttt                           <  2:5079716/93‑1 (MQ=255)

                                                               tttAAGATCCATGTCTCAATTTTCTCGTGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtg                          >  2:8806053/1‑93 (MQ=255)

                                                               tttAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtg                          <  1:801014/93‑1 (MQ=255)

                                                                ttAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtgt                         >  1:6634299/1‑93 (MQ=255)

                                                                 tAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCTATAGTGTTTTTCGGCAAGAGCGCTGGGTTtgtg                        <  1:4168810/93‑1 (MQ=255)

                                                                  aaGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTgtgt                       <  2:7990435/93‑1 (MQ=255)

                                                                  aaGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTgtgt                       <  1:1136808/93‑1 (MQ=255)

                                                                   aGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTc                      <  2:8148396/93‑1 (MQ=255)

                                                                       ccATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCAt                  >  2:2467297/1‑93 (MQ=255)

                                                                        cATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATa                 <  1:10008079/93‑1 (MQ=255)

                                                                        cATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATa                 >  1:9058142/1‑93 (MQ=255)

                                                                        cATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGGCCCATa                 <  2:1836565/93‑1 (MQ=255)

                                                                        cATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGAGTTTTTCTGCAAGAGCGCTTGGTTTGTGTCCCaca                 >  2:9105971/1‑91 (MQ=255)

                                                                          tGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAAt               >  2:9638824/1‑93 (MQ=255)

                                                                           gTCTCAATTTTCTCGCGCGATTTTCGTAACAGATCTAACGGACGACGTCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAAtt              <  1:535245/93‑1 (MQ=255)

                                                                             ctcAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTg            <  1:5845464/93‑1 (MQ=255)

                                                                              tcAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGa           <  2:4837685/93‑1 (MQ=255)

                                                                              tcAATTTTCTCGCGCCATTTTAGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGa           >  2:5385544/1‑93 (MQ=255)

                                                                                aaTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGc         <  1:9152247/93‑1 (MQ=255)

                                                                                aaTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGc         <  2:1065945/93‑1 (MQ=255)

                                                                                 aTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGca        >  1:1278899/1‑93 (MQ=255)

                                                                                   tttCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGcaac      >  1:9485124/1‑93 (MQ=255)

                                                                                   tttCTCGCGCCATTTTAGTAACAGAGCTAACGGACGGCGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGcaac      <  1:2737948/93‑1 (MQ=255)

                                                                                    ttCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGACAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGcaaca     <  1:4481996/93‑1 (MQ=255)

                                                                                     tctcGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGcaacaa    <  1:1410889/93‑1 (MQ=255)

TATGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGTGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGCAACAATA  >  NC_000913/294900‑295079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`