Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,449,280 T→C T109A (ACC→GCC)  b2335 ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,2800TC99.4% 1065.3 / ‑4.3 334T109A (ACC→GCC) b2335putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (151/181);  total (152/182)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 348 total aligned reads displayed.

GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC  >  NC_000913/2449190‑2449369
                                                                                          |                                                                                         
ggTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGa                                                                                         <  1:4668158/93‑1 (MQ=255)
 gTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGat                                                                                        >  2:364364/1‑93 (MQ=255)
    aTTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATAtt                                                                                     <  1:9514014/93‑1 (MQ=255)
      tGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTgg                                                                                   <  1:3890990/93‑1 (MQ=255)
      tGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTgg                                                                                   >  2:5721091/1‑93 (MQ=255)
       gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGt                                                                                  >  2:3127605/1‑93 (MQ=255)
       gATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGt                                                                                  >  1:3623717/1‑93 (MQ=255)
        atatTAAATGGCGTCTAGCTATTTCCGCCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  2:5199281/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  1:3042438/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  2:4827164/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  1:2157086/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  2:1825672/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  1:174431/93‑1 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATCggga                                                                                 >  2:2236931/1‑91 (MQ=255)
        atatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGATATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTa                                                                                 <  2:6611439/93‑1 (MQ=255)
         tatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTAc                                                                                <  1:9816728/93‑1 (MQ=255)
         tatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTAc                                                                                >  2:8334788/1‑93 (MQ=255)
         tatTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTAc                                                                                <  2:1503188/93‑1 (MQ=255)
           ttAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTg                                                                              <  2:491188/93‑1 (MQ=255)
           ttAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTg                                                                              >  2:8778741/1‑93 (MQ=255)
            tAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGa                                                                             <  2:6351865/93‑1 (MQ=255)
            tAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGa                                                                             <  1:7239701/93‑1 (MQ=255)
             aaaTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAg                                                                            <  1:8184996/93‑1 (MQ=255)
               aTGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTa                                                                          >  1:5889720/1‑93 (MQ=255)
                 ggCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTAcc                                                                        >  1:7762896/1‑93 (MQ=255)
                  gCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCg                                                                       >  2:8254033/1‑93 (MQ=255)
                   gctCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGt                                                                      <  1:1490052/91‑1 (MQ=255)
                    gTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGtt                                                                     <  1:7636583/93‑1 (MQ=255)
                     tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGttt                                                                    >  1:9958698/1‑93 (MQ=255)
                     tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGttt                                                                    >  2:2547999/1‑93 (MQ=255)
                     tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGttt                                                                    <  2:4449554/93‑1 (MQ=255)
                     tCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGttt                                                                    >  1:8487411/1‑93 (MQ=255)
                      ccAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTc                                                                   >  1:6215403/1‑93 (MQ=255)
                      ccAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTc                                                                   <  2:8634068/93‑1 (MQ=255)
                        aGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATCCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGc                                                                 >  2:5029929/1‑93 (MQ=255)
                          atATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTAACCGAATGCCCAGCCCGGCGATATTGGTACTGAGTTCCGTTTCGACg                                                               <  2:9265678/92‑1 (MQ=255)
                           tATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGt                                                              <  2:9834704/93‑1 (MQ=255)
                             ttttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTa                                                            <  1:3089265/93‑1 (MQ=255)
                             ttttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTa                                                            >  2:9591814/1‑93 (MQ=255)
                             ttttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTa                                                            <  2:5446407/93‑1 (MQ=255)
                              tttCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTaa                                                           >  2:6442497/1‑93 (MQ=255)
                                tCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATc                                                         >  1:7504804/1‑93 (MQ=255)
                                 cACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCg                                                        <  2:3422879/93‑1 (MQ=255)
                                  aCCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGt                                                       >  1:2665581/1‑93 (MQ=255)
                                        ggCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGt                                                 <  2:6930577/93‑1 (MQ=255)
                                         gCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAAGGCCCAGCCCGGCGATATTGGTACTGGGTACCGTTTCGCCGTTAATCGTTGAGGTa                                                <  2:3262021/93‑1 (MQ=255)
                                          cAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAt                                               <  2:1663117/93‑1 (MQ=255)
                                          cAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAt                                               >  2:2116129/1‑93 (MQ=255)
                                          cAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAt                                               <  1:8093704/93‑1 (MQ=255)
                                           aaaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAtt                                              >  2:3847438/1‑93 (MQ=255)
                                           aaaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTAtt                                              <  1:5266960/93‑1 (MQ=255)
                                            aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                             >  1:9704892/1‑93 (MQ=255)
                                            aaCAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTg                                             >  2:4658368/1‑93 (MQ=255)
                                               aaTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGccc                                          <  1:6541984/93‑1 (MQ=255)
                                               aaTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGccc                                          <  2:9771369/93‑1 (MQ=255)
                                                aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                         >  2:7910603/1‑93 (MQ=255)
                                                aTGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCt                                         >  2:2991051/1‑93 (MQ=255)
                                                  gAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTa                                       <  2:737421/93‑1 (MQ=255)
                                                  gAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTa                                       >  2:8828434/1‑93 (MQ=255)
                                                   aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  2:574333/93‑1 (MQ=255)
                                                   aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  1:3718555/93‑1 (MQ=255)
                                                   aGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTaa                                      <  1:5672387/93‑1 (MQ=255)
                                                    gTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAg                                     <  2:3386062/93‑1 (MQ=255)
                                                     ttATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGt                                    <  1:5179223/93‑1 (MQ=255)
                                                     ttATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGt                                    >  1:1343028/1‑93 (MQ=255)
                                                     ttATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGt                                    <  1:8554283/93‑1 (MQ=255)
                                                      tATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                                   <  1:4792792/93‑1 (MQ=255)
                                                      tATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGtt                                   <  1:2052984/93‑1 (MQ=255)
                                                        tCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGc                                 <  2:8430420/93‑1 (MQ=255)
                                                         cGGCGCTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCa                                <  2:7716951/93‑1 (MQ=255)
                                                           gcgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                              >  2:5177079/1‑93 (MQ=255)
                                                           gcgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATc                              <  1:6408818/93‑1 (MQ=255)
                                                            cgcTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATcc                             <  2:6714172/93‑1 (MQ=255)
                                                              cTATTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGc                           >  2:6782840/1‑93 (MQ=255)
                                                                tttttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCaa                         <  1:9219444/92‑1 (MQ=255)
                                                                aTTTTCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCaa                         <  1:4234816/93‑1 (MQ=255)
                                                                 ttttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAg                        >  1:2882555/1‑93 (MQ=255)
                                                                  tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  2:8003388/93‑1 (MQ=255)
                                                                  tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  1:3795229/93‑1 (MQ=255)
                                                                  tttCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGt                       <  1:1197954/93‑1 (MQ=255)
                                                                    tCTATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCg                     >  1:6916946/1‑93 (MQ=255)
                                                                      tATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGtt                   <  1:1025392/93‑1 (MQ=255)
                                                                      tATCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGtt                   <  2:6041994/93‑1 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  >  1:7952041/1‑93 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  <  2:5600247/93‑1 (MQ=255)
                                                                       aTCCGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTa                  <  2:4114282/93‑1 (MQ=255)
                                                                          cGAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCa               >  1:8999255/1‑93 (MQ=255)
                                                                           gAATGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCaa              <  1:5354733/93‑1 (MQ=255)
                                                                            aaTGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAg             >  1:6765607/1‑93 (MQ=255)
                                                                            aaTGCCCAGCCAGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAg             >  1:2096889/1‑93 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           <  1:3511060/93‑1 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           <  1:5458125/93‑1 (MQ=255)
                                                                              tGCCCAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGAc           >  2:6266514/1‑93 (MQ=255)
                                                                                cccAGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTg         >  1:4928182/1‑93 (MQ=255)
                                                                                   cgCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      <  1:3364692/92‑1 (MQ=255)
                                                                                   aGCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGttt      <  2:7612613/93‑1 (MQ=255)
                                                                                    gCCCGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTtg     <  1:2280380/93‑1 (MQ=255)
                                                                                      ccGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTtgtg   <  1:6727479/93‑1 (MQ=255)
                                                                                       cGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGc  >  2:6559758/1‑93 (MQ=255)
                                                                                       cGGCGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGc  >  2:7053458/1‑93 (MQ=255)
                                                                                          |                                                                                         
GGTTATTGATATTAAATGGCGTCCAGCTATTTTCACCGACGGCAAACAATGAGTTATCGGCGCTATTTTCTATCCGAATGCCCAGCCCGGTGATATTGGTACTGAGTACCGTTTCGCCGTTAATCGTTGAGGTATTGCCCTTAAGTTGCATCCGCAAGTCGTTAGCAAGACTGTTTGTGC  >  NC_000913/2449190‑2449369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑