BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,461,706	T→C	S225S (AGT→AGC)	b2345 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,461,706	0	T	C	99.8%	1602.2 / ‑5.2	490	S225S (AGT→AGC)	b2345	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (243/246); total (243/247)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.70e-01

Only 100 of 497 total aligned reads displayed.

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

ttACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGa                                                                                         >  2:778269/1‑93 (MQ=255)

ttACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGa                                                                                         >  1:6924618/1‑93 (MQ=255)

 tACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaa                                                                                        >  2:2805031/1‑93 (MQ=255)

 tACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaa                                                                                        >  1:8187249/1‑93 (MQ=255)

  aCTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaa                                                                                       >  1:7664986/1‑93 (MQ=255)

   cctATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  2:1963284/91‑1 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  1:108136/93‑1 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      >  2:9787990/1‑93 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      >  2:9278677/1‑93 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      <  2:8886687/93‑1 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      >  2:3962781/1‑93 (MQ=255)

   cTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGaaaa                                                                                      >  1:6182579/1‑93 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     <  1:6552224/93‑1 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAAc                                                                                     >  2:209619/1‑93 (MQ=255)

    ttATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTATCTCCCAATATTGTAGCGAAAAc                                                                                     <  2:6708652/93‑1 (MQ=255)

     tATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACg                                                                                    >  1:7756315/1‑93 (MQ=255)

       tctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGcc                                                                                  <  2:4403353/93‑1 (MQ=255)

        ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCa                                                                                 <  2:768861/93‑1 (MQ=255)

        ctctcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCa                                                                                 <  1:5822696/93‑1 (MQ=255)

           tcGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGa                                                                              <  1:90726/93‑1 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  2:5644941/1‑93 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  2:8702067/1‑93 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  2:3060330/1‑93 (MQ=255)

              gTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTg                                                                           >  1:9887458/1‑93 (MQ=255)

               ttACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGc                                                                          >  2:5220997/1‑93 (MQ=255)

                tactacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCt                                                                         <  2:5941064/93‑1 (MQ=255)

                  ctacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTc                                                                       >  1:154543/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      >  2:2454081/1‑93 (MQ=255)

                   tacGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCa                                                                      >  1:1627722/1‑93 (MQ=255)

                    acGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAg                                                                     >  2:4497244/1‑93 (MQ=255)

                      gATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCg                                                                   <  1:3108087/93‑1 (MQ=255)

                       aTCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGt                                                                  >  1:5522786/1‑93 (MQ=255)

                       aTCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGt                                                                  >  1:3519236/1‑93 (MQ=255)

                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 <  2:8330768/93‑1 (MQ=255)

                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 <  1:3369388/93‑1 (MQ=255)

                        tCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTg                                                                 <  2:2908884/93‑1 (MQ=255)

                          aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGt                                                               <  1:1858206/93‑1 (MQ=255)

                          aGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGt                                                               >  1:836893/1‑93 (MQ=255)

                           ggACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGtt                                                              >  2:372036/1‑93 (MQ=255)

                            gacgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGttt                                                             >  1:10015400/1‑93 (MQ=255)

                            gacgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGttt                                                             <  2:8267868/93‑1 (MQ=255)

                              cgacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGa                                                           <  1:3711297/93‑1 (MQ=255)

                               gacTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaa                                                          <  1:6786950/93‑1 (MQ=255)

                                acTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaaa                                                         <  1:2329934/93‑1 (MQ=255)

                                acTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGaaa                                                         <  1:6361204/93‑1 (MQ=255)

                                 cTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAg                                                        >  2:2572318/1‑93 (MQ=255)

                                  tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                       >  2:7963999/1‑93 (MQ=255)

                                  tACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGc                                                       >  1:9950293/1‑93 (MQ=255)

                                   aCCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCa                                                      <  1:7948321/93‑1 (MQ=255)

                                     cacaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGt                                                    <  1:1762978/93‑1 (MQ=255)

                                     cacaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGt                                                    >  1:2640499/1‑93 (MQ=255)

                                     cacaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGt                                                    >  2:405581/1‑93 (MQ=255)

                                      acaTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTa                                                   >  1:2536868/1‑93 (MQ=255)

                                       caTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaa                                                  <  1:5360444/93‑1 (MQ=255)

                                        aTTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTaaa                                                 <  1:8734938/93‑1 (MQ=255)

                                         ttCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAg                                                >  2:8637830/1‑93 (MQ=255)

                                             aaGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTAt                                            >  2:1552343/1‑93 (MQ=255)

                                              agagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATc                                           <  1:7116622/93‑1 (MQ=255)

                                               gagGAATTCGTATTGATGGTGATAGTCGCACCCACTATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          <  1:9722326/93‑1 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  2:2171866/1‑93 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          <  2:5058188/93‑1 (MQ=255)

                                               gagGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCa                                          >  1:4523547/1‑93 (MQ=255)

                                                 ggAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATg                                        >  2:7360929/1‑93 (MQ=255)

                                                   aaTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGc                                      >  2:7052455/1‑93 (MQ=255)

                                                   aaTTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGc                                      <  1:921586/93‑1 (MQ=255)

                                                     ttCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAg                                    >  2:9064700/1‑93 (MQ=255)

                                                     ttCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAg                                    >  1:5926202/1‑93 (MQ=255)

                                                      tCGTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGa                                   <  2:330479/93‑1 (MQ=255)

                                                        gTATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAAt                                 <  2:8118890/93‑1 (MQ=255)

                                                         tATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATc                                >  1:7428166/1‑93 (MQ=255)

                                                         tATTAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATc                                >  2:1117055/1‑93 (MQ=255)

                                                           ttAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAt                              <  1:952424/93‑1 (MQ=255)

                                                            tAATGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCAtt                             <  1:439874/93‑1 (MQ=255)

                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTTGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           <  2:1250096/93‑1 (MQ=255)

                                                              aTGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTg                           >  2:3849657/1‑93 (MQ=255)

                                                               tGGTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGc                          >  1:2044532/1‑93 (MQ=255)

                                                                ggTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCa                         <  2:540633/93‑1 (MQ=255)

                                                                 gTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaa                        >  1:2818996/1‑93 (MQ=255)

                                                                 gTGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCaa                        >  2:4975677/1‑93 (MQ=255)

                                                                  tGATAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAAc                       >  2:3168915/1‑93 (MQ=255)

                                                                   gATAGTCTCACCCAATATCGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACt                      >  2:3510921/1‑93 (MQ=255)

                                                                     tAGTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                    >  1:3234882/1‑93 (MQ=255)

                                                                     tAGTCTCACCCAATATTGAAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTgg                    <  1:8931328/93‑1 (MQ=255)

                                                                       gTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGtt                  <  1:1276533/93‑1 (MQ=255)

                                                                       gTCTCACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGtt                  >  2:4630056/1‑93 (MQ=255)

                                                                        tctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTc                 >  2:6370745/1‑93 (MQ=255)

                                                                        tctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTc                 <  1:1357496/93‑1 (MQ=255)

                                                                        tctcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTc                 <  1:4110748/93‑1 (MQ=255)

                                                                          tcACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCg               <  2:624999/93‑1 (MQ=255)

                                                                           cACCCAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGa              >  1:2188873/1‑93 (MQ=255)

                                                                             cccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc            >  2:2371482/1‑93 (MQ=255)

                                                                             cccAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatc            >  1:4773818/1‑93 (MQ=255)

                                                                               cAATATTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGatcat          <  2:5738249/93‑1 (MQ=255)

                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        >  1:4259196/1‑93 (MQ=255)

                                                                                 atatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGAtcatca        <  1:9349533/93‑1 (MQ=255)

                                                                                  tatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAg       <  1:8878527/93‑1 (MQ=255)

                                                                                  tatTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAg       >  2:6535654/1‑93 (MQ=255)

                                                                                   atTGTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAgg      >  2:9576826/1‑93 (MQ=255)

                                                                                      gTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTg   >  1:299719/1‑93 (MQ=255)

                                                                                      gTAGCGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTg   <  2:6286341/93‑1 (MQ=255)

TTACTTATCTCTCGGTTACTACGATCAGGACGACTACCACATTCAAAGAGGAATTCGTATTAATGGTGATAGTCTCACCCAATATTGTAGTGAAAACGCCAGGAGTGCTTCAGCGTGGTTTGAAAGCAGTAAAGCTATCATGGCAGAATCATTTGCAACTGGTTCCGATCATCAGGTTGT  >  NC_000913/2461616‑2461795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`