BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,470,298	A→G	I37T (ATT→ACT)	b2356 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,470,298	0	A	G	99.6%	895.9 / ‑5.0	275	I37T (ATT→ACT)	b2356	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (161/113); total (162/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 279 total aligned reads displayed.

GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC  >  NC_000913/2470208‑2470387

gcgTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGtt                                                                                         <  1:2643216/93‑1 (MQ=255)

gcgTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGtt                                                                                         >  2:7806198/1‑93 (MQ=255)

  gTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTcc                                                                                       >  1:4971910/1‑93 (MQ=255)

  gTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGAGTTAGTTcc                                                                                       >  2:3129990/1‑93 (MQ=255)

     gTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCa                                                                                    <  1:886233/93‑1 (MQ=255)

        ttCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGaa                                                                                 >  2:5984063/1‑93 (MQ=255)

          ccGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGaaaa                                                                               <  1:9178342/93‑1 (MQ=255)

            gCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtg                                                                             >  2:7756197/1‑93 (MQ=255)

             cAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTGCCCAGAAAAtgt                                                                            >  1:5353776/1‑93 (MQ=255)

             cAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgt                                                                            <  2:8935858/93‑1 (MQ=255)

              aGTGTAATACGCGGCACGTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           <  1:3507638/93‑1 (MQ=255)

              aGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           >  2:4504896/1‑93 (MQ=255)

              aGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgtt                                                                           <  1:1634872/93‑1 (MQ=255)

               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  1:5559069/93‑1 (MQ=255)

               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  2:7929894/93‑1 (MQ=255)

               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          <  1:6708822/93‑1 (MQ=255)

               gtgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttg                                                                          >  2:5881521/1‑93 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  2:4415463/1‑93 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  1:5951885/1‑93 (MQ=255)

                tgtAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAAtgttgt                                                                         >  2:9054616/1‑93 (MQ=255)

                  tAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTcg                                                                       >  2:3550042/1‑92 (MQ=255)

                  tAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTcc                                                                       <  2:5776261/93‑1 (MQ=255)

                     tACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGc                                                                    >  1:6538599/1‑93 (MQ=255)

                      aCGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCg                                                                   <  1:578462/93‑1 (MQ=255)

                        gcgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTg                                                                 <  1:2825344/93‑1 (MQ=255)

                         cgGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgc                                                                <  2:7123034/93‑1 (MQ=255)

                          ggCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcg                                                               >  2:2820195/1‑93 (MQ=255)

                          ggCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcg                                                               >  2:506004/1‑93 (MQ=255)

                           gCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTgcgc                                                              >  1:9926600/1‑93 (MQ=255)

                            cacaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGcc                                                             >  1:7531474/1‑93 (MQ=255)

                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            <  2:6506874/93‑1 (MQ=255)

                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            >  2:2182790/1‑93 (MQ=255)

                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            >  1:7778795/1‑93 (MQ=255)

                             acaTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCa                                                            >  1:7677805/1‑93 (MQ=255)

                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  2:105545/93‑1 (MQ=255)

                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  1:144599/93‑1 (MQ=255)

                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCAc                                                           <  1:7303828/93‑1 (MQ=255)

                              caTTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGCTCCCCAGACAATGTTGACCGGCGTGCGCCAc                                                           >  2:6534356/1‑93 (MQ=255)

                                 ttGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGa                                                        >  1:4907592/1‑93 (MQ=255)

                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       <  2:522773/93‑1 (MQ=255)

                                  tGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGAt                                                       <  2:3061035/93‑1 (MQ=255)

                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  2:1717397/1‑93 (MQ=255)

                                      gTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGcc                                                   >  1:4309338/1‑93 (MQ=255)

                                       ttATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGccc                                                  >  2:5352921/1‑93 (MQ=255)

                                         aTCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAc                                                <  2:4055424/93‑1 (MQ=255)

                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               >  2:415275/1‑93 (MQ=255)

                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               >  1:1014425/1‑93 (MQ=255)

                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               <  1:9387782/93‑1 (MQ=255)

                                          tCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAct                                               >  1:9088607/1‑93 (MQ=255)

                                           cACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActt                                              >  2:5421915/1‑93 (MQ=255)

                                            aCCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCAtttc                                             >  2:2736154/1‑93 (MQ=255)

                                             ccATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcc                                            >  2:9215908/1‑93 (MQ=255)

                                              cATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcct                                           >  1:4817225/1‑93 (MQ=255)

                                               aTCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtcctt                                          <  2:8699558/93‑1 (MQ=255)

                                                tCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCActtccttc                                         >  2:1414194/1‑93 (MQ=255)

                                                 cAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCa                                        <  1:576663/93‑1 (MQ=255)

                                                  aGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAg                                       >  2:7712702/1‑93 (MQ=255)

                                                   gTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGt                                      >  2:4345875/1‑93 (MQ=255)

                                                    tAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGtt                                     >  2:4575062/1‑93 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  1:3808938/1‑93 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  1:7999877/1‑93 (MQ=255)

                                                       aCAGATCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCAt                                  >  1:1712174/1‑93 (MQ=255)

                                                           aTCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGc                              <  1:3079799/93‑1 (MQ=255)

                                                           aTCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGc                              >  2:4716946/1‑93 (MQ=255)

                                                            tCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCt                             >  2:968857/1‑93 (MQ=255)

                                                            tCCAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCt                             <  1:1795594/93‑1 (MQ=255)

                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCtc                            >  1:7104554/1‑92 (MQ=255)

                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTg                            <  2:3342218/93‑1 (MQ=255)

                                                             ccAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTg                            >  1:2437779/1‑93 (MQ=255)

                                                              cAGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTgg                           >  1:2986365/1‑93 (MQ=255)

                                                               aGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGt                          <  2:493637/93‑1 (MQ=255)

                                                               aGAACAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGt                          >  1:9773292/1‑93 (MQ=255)

                                                                 aaCAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGttt                        >  2:3940065/1‑93 (MQ=255)

                                                                 aaCAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGttt                        >  1:4058256/1‑93 (MQ=255)

                                                                 aaCAAACGGGCCAAACAGGGAGTTAGTTTCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATAGCCCACTTCCTTCAGTTCATGCGATGGgtt                        >  2:3760304/1‑93 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      >  1:3386150/1‑93 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      >  1:6272346/1‑93 (MQ=255)

                                                                   cAAACGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGt                      <  1:8584815/93‑1 (MQ=255)

                                                                    aaaCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGtt                     >  2:9383748/1‑93 (MQ=255)

                                                                    aaaCGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGtt                     >  1:6193251/1‑93 (MQ=255)

                                                                     aaCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTc                    >  1:2348638/1‑93 (MQ=255)

                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   >  1:2465220/1‑93 (MQ=255)

                                                                      aCGGGCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTcc                   >  1:2007443/1‑93 (MQ=255)

                                                                        gggCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGc                 <  1:1151275/93‑1 (MQ=255)

                                                                         ggCCAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCa                <  2:6137506/93‑1 (MQ=255)

                                                                            cAAACAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCt             >  2:2465842/1‑93 (MQ=255)

                                                                             aaaCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTc            <  2:2152306/93‑1 (MQ=255)

                                                                               aCAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTcca          >  2:1793116/1‑93 (MQ=255)

                                                                                cAGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCGCTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccac         <  1:8129891/93‑1 (MQ=255)

                                                                                 aGGGTGTTAGTTCCCCGGAAAATGGTGTCCGGCGTGCGCCACTCATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  1:5305384/93‑1 (MQ=255)

                                                                                 aGGGTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacc        <  2:567140/93‑1 (MQ=255)

                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       <  2:7482422/93‑1 (MQ=255)

                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       <  1:95595/93‑1 (MQ=255)

                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       >  1:5284967/1‑93 (MQ=255)

                                                                                  gggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTccacca       <  2:997129/93‑1 (MQ=255)

                                                                                   ggTGTTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAg      <  1:5022496/93‑1 (MQ=255)

                                                                                    gtgtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgc     <  1:5074595/93‑1 (MQ=255)

                                                                                     tgtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgcc    >  2:7345919/1‑92 (MQ=255)

                                                                                      gtTAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAgcgc   <  2:7179740/93‑1 (MQ=255)

                                                                                       ttAGTTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGCTTGGTCCGCAGCTCCACCCGCGcc  >  2:2636510/1‑93 (MQ=255)

GCGTTGTCTTCCGCAGTGTAATACGCGGCACATTTGGCGTTATCACCATCAGTAAACAGATCCAGAACAAACGGGCCAAACAGGGTGTTAATTCCCCAGAAAATGTTGTCCGGCGTGCGCCACTGATCGCCCACTTCCTTCAGTTCATGGGCTGGTTTGTTCCGCAGCTCCACCAGCGCC  >  NC_000913/2470208‑2470387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`