BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,596,026	T→C	D287G (GAC→GGC)	nlpB ←	lipoprotein‑34

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,596,026	0	T	C	95.4%	826.3 / 17.3	285	D287G (GAC→GGC)	nlpB	lipoprotein‑34
Reads supporting (aligned to +/- strand): ref base T (4/9); new base C (118/153); total (123/162)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 294 total aligned reads displayed.

TGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCC  >  NC_000913/2595936‑2596115

tGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCg                                                                                         >  2:1197323/1‑93 (MQ=255)

tGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCg                                                                                         >  2:6703659/1‑93 (MQ=255)

 gTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGc                                                                                        >  1:1251837/1‑93 (MQ=255)

 gTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGc                                                                                        <  2:7240375/93‑1 (MQ=255)

  tAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCt                                                                                       <  2:4373061/93‑1 (MQ=255)

    ggCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGt                                                                                     <  1:4773179/93‑1 (MQ=255)

    ggCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTCTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTGCTGCCAGCCGCTGt                                                                                     <  1:7393052/93‑1 (MQ=255)

     gctgctGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTc                                                                                    <  2:1439884/93‑1 (MQ=255)

      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   <  1:2252468/93‑1 (MQ=255)

      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   <  1:9470544/93‑1 (MQ=255)

      ctgctgCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   >  1:2534098/1‑93 (MQ=255)

      ctgctgCGGTTATCTAAATCGCAAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCa                                                                                   <  2:8415444/93‑1 (MQ=255)

         ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAc                                                                                <  2:6331338/93‑1 (MQ=255)

         ctgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAc                                                                                >  1:9599985/1‑93 (MQ=255)

         ctgcGGGTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGAc                                                                                <  1:3626567/93‑1 (MQ=255)

          tgcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACa                                                                               >  1:2763070/1‑93 (MQ=255)

          tgcGGTGATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACa                                                                               <  2:4233480/93‑1 (MQ=255)

            cGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGc                                                                             <  1:7822114/93‑1 (MQ=255)

            cGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGc                                                                             <  1:9159840/93‑1 (MQ=255)

             ggTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCg                                                                            >  1:1951084/1‑93 (MQ=255)

               ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCCGACAGCGGc                                                                          >  2:8523112/1‑93 (MQ=255)

               ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGc                                                                          <  1:8164146/93‑1 (MQ=255)

                 aTCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTGAGACAGCGGCtt                                                                        >  2:190001/1‑93 (MQ=255)

                 aTCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCtt                                                                        <  1:9319013/93‑1 (MQ=255)

                  tCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTta                                                                       <  2:5440672/93‑1 (MQ=255)

                   cTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTtat                                                                      <  1:3120397/93‑1 (MQ=255)

                    tAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTtata                                                                     >  1:8525700/1‑93 (MQ=255)

                     aaaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    >  2:6448100/1‑93 (MQ=255)

                     aaaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    >  2:1141159/1‑93 (MQ=255)

                     aaaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    <  1:537937/93‑1 (MQ=255)

                     aaaTCGCCAACCTCCGGTTTATAGTCGCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATaa                                                                    <  1:6078571/93‑1 (MQ=255)

                       aTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGt                                                                  >  2:5969661/1‑93 (MQ=255)

                       aTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGt                                                                  <  2:3704190/93‑1 (MQ=255)

                         cGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTa                                                                <  1:6232084/93‑1 (MQ=255)

                         cGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTa                                                                <  1:402689/93‑1 (MQ=255)

                          gCCAACCTGCAGTTTATAGTCACCGGAGGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTAc                                                               <  1:4287181/93‑1 (MQ=255)

                           ccAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACg                                                              <  1:5159772/93‑1 (MQ=255)

                             aaCCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGc                                                            <  2:6075024/93‑1 (MQ=255)

                              aCCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGcc                                                           >  2:676792/1‑93 (MQ=255)

                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          <  2:2428274/93‑1 (MQ=255)

                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          <  2:8168064/93‑1 (MQ=255)

                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          >  1:8477741/1‑93 (MQ=255)

                               ccTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                                          >  2:303180/1‑93 (MQ=255)

                                cTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAt                                                         >  2:8803905/1‑93 (MQ=255)

                                cTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCAt                                                         <  1:671827/93‑1 (MQ=255)

                                 tgtaGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATg                                                        <  1:1013884/90‑1 (MQ=255)

                                 tGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATg                                                        <  2:25205/93‑1 (MQ=255)

                                  gCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGt                                                       <  2:7711702/93‑1 (MQ=255)

                                      tttATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGcc                                                   >  2:7433704/1‑93 (MQ=255)

                                      tttATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGGTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTggc                                                   >  2:9610636/1‑91 (MQ=255)

                                       ttATAGTCACCGGATGTCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGccc                                                  <  1:7884013/93‑1 (MQ=255)

                                       ttATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGccc                                                  <  1:4578327/93‑1 (MQ=255)

                                        tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCt                                                 <  1:6978509/93‑1 (MQ=255)

                                        tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCt                                                 >  2:7377116/1‑93 (MQ=255)

                                        tataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCt                                                 <  2:5062805/93‑1 (MQ=255)

                                         ataGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTg                                                >  1:8712864/1‑93 (MQ=255)

                                            gTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGa                                             >  1:4440123/1‑93 (MQ=255)

                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            <  1:1326083/93‑1 (MQ=255)

                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            <  1:8583174/93‑1 (MQ=255)

                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            <  1:3572442/93‑1 (MQ=255)

                                             tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGaa                                            >  2:4972813/1‑93 (MQ=255)

                                              cACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAAc                                           <  1:3440006/93‑1 (MQ=255)

                                                ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACgg                                         >  2:5766158/1‑93 (MQ=255)

                                                ccGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACgg                                         >  1:8902308/1‑93 (MQ=255)

                                                   gATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTg                                      >  1:9758477/1‑93 (MQ=255)

                                                    aTGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGc                                     <  1:6444928/93‑1 (MQ=255)

                                                    aTGCCAGCCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGc                                     <  2:9127065/93‑1 (MQ=255)

                                                          ggCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGGTGTCgg                               >  2:9160521/1‑93 (MQ=255)

                                                          ggCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCgg                               <  2:5462883/93‑1 (MQ=255)

                                                           gCCTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGt                              >  1:3954590/1‑93 (MQ=255)

                                                            ccTGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTc                             >  1:753167/1‑93 (MQ=255)

                                                              tgaaTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCAc                           <  1:5009990/90‑1 (MQ=255)

                                                              tGGATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGGACGGGTGCTGGCGGTCAc                           >  2:7309916/1‑93 (MQ=255)

                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          >  1:2106466/1‑93 (MQ=255)

                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          <  1:9971569/93‑1 (MQ=255)

                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          >  2:7832649/1‑93 (MQ=255)

                                                               ggATCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACt                          >  2:4624199/1‑93 (MQ=255)

                                                                gATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACtt                         <  1:1833605/93‑1 (MQ=255)

                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        >  2:3598630/1‑93 (MQ=255)

                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        <  2:4019015/93‑1 (MQ=255)

                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        >  2:3193654/1‑93 (MQ=255)

                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt                        <  2:3421653/93‑1 (MQ=255)

                                                                 aTCGCTCGCGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGCGCTGTCGGTCACttt                        >  2:5013967/1‑93 (MQ=255)

                                                                   cgctcgcGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCa                      <  1:5563186/93‑1 (MQ=255)

                                                                   cgctcgcGCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCa                      <  1:1691708/93‑1 (MQ=255)

                                                                       cgcgCCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGcc                  <  1:617504/93‑1 (MQ=255)

                                                                         cgcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGTTGTCGGTCACTTTCATGCCCa                <  2:5210078/93‑1 (MQ=255)

                                                                         cgcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCa                <  2:2731524/93‑1 (MQ=255)

                                                                         cgcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCa                <  1:1596786/93‑1 (MQ=255)

                                                                          gcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAc               >  1:8823617/1‑93 (MQ=255)

                                                                          gcCCAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACAGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCAc               >  2:2820566/1‑93 (MQ=255)

                                                                            ccAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACtt             <  1:6483144/93‑1 (MQ=255)

                                                                             cAGTTCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACttt            >  1:5746923/1‑93 (MQ=255)

                                                                                 tCCTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTcc        <  2:9577633/93‑1 (MQ=255)

                                                                                  ccTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCa       <  2:3689474/93‑1 (MQ=255)

                                                                                  ccTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCa       <  2:9366343/93‑1 (MQ=255)

                                                                                  ccTGCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCa       <  2:9551308/93‑1 (MQ=255)

                                                                                     gCCAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAgcg    >  2:2296836/1‑93 (MQ=255)

                                                                                      ccAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAgcgc   >  2:7054829/1‑93 (MQ=255)

                                                                                       cAGCCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGcgcc  >  2:9385635/1‑93 (MQ=255)

TGTAGGCTGCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTTCATGCCCACTTTTTCCAGCGCC  >  NC_000913/2595936‑2596115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`