BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,613,076	T→C	T276A (ACT→GCT)	perM ←	putative permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,613,076	0	T	C	99.6%	784.6 / ‑5.2	242	T276A (ACT→GCT)	perM	putative permease
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (120/121); total (120/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01

Only 100 of 245 total aligned reads displayed.

TAACCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGTACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGCCG  >  NC_000913/2612986‑2613164

tAACCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCAc                                                                                        <  1:8037591/93‑1 (MQ=255)

tAACCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCAc                                                                                        <  2:2820692/93‑1 (MQ=255)

 aaCCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCAcc                                                                                       <  1:4246505/93‑1 (MQ=255)

 aaCCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCAcc                                                                                       <  1:7046324/93‑1 (MQ=255)

 aaCCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCAcc                                                                                       <  1:2681641/93‑1 (MQ=255)

  aCCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCt                                                                                      <  2:4481305/93‑1 (MQ=255)

  aCCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCt                                                                                      <  2:5434146/93‑1 (MQ=255)

   ccGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTg                                                                                     >  2:4683893/1‑93 (MQ=255)

       ttCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGGGcc                                                                                 >  2:982911/1‑93 (MQ=255)

       ttCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGcc                                                                                 >  2:7257150/1‑93 (MQ=255)

       ttCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGcc                                                                                 >  2:3632339/1‑93 (MQ=255)

        tCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCGGCGCCTGAATAATCAGATACAACGCGAAGCAGCTCCAGAATTCAGCACCTGAGCCa                                                                                >  1:5392318/1‑93 (MQ=255)

         cGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCaa                                                                               >  2:1734766/1‑93 (MQ=255)

         cGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCaa                                                                               >  1:7800130/1‑93 (MQ=255)

          ggAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCaaa                                                                              <  2:7133859/93‑1 (MQ=255)

          ggAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCaaa                                                                              >  1:2561021/1‑93 (MQ=255)

          ggAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCaaa                                                                              <  1:6767210/93‑1 (MQ=255)

           gagaACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAAc                                                                             >  1:594606/1‑93 (MQ=255)

           gagaACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAAc                                                                             <  1:1026856/93‑1 (MQ=255)

                caacaCCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGaa                                                                        >  1:5642529/1‑93 (MQ=255)

                  acacCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATa                                                                      <  2:1269268/93‑1 (MQ=255)

                  acacCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATa                                                                      <  1:3497520/93‑1 (MQ=255)

                    acCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAgc                                                                    >  1:8898595/1‑93 (MQ=255)

                    acCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAgc                                                                    >  1:223063/1‑93 (MQ=255)

                        gCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGcgcca                                                                <  1:4286375/93‑1 (MQ=255)

                        gCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGcgcca                                                                <  1:1445189/93‑1 (MQ=255)

                           cTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGcgccacgc                                                             <  1:9491706/93‑1 (MQ=255)

                           cTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGcgccacgc                                                             >  1:285548/1‑93 (MQ=255)

                            tAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGcgccacgcc                                                            >  2:8134919/1‑93 (MQ=255)

                            tAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGcgccacgcc                                                            >  1:9347242/1‑93 (MQ=255)

                               cAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAac                                                         >  1:4129138/1‑93 (MQ=255)

                               cAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAac                                                         >  1:8085951/1‑93 (MQ=255)

                               cAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAac                                                         >  2:8375035/1‑93 (MQ=255)

                                aGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAacc                                                        <  1:2283451/93‑1 (MQ=255)

                                aGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAacc                                                        <  2:5874504/93‑1 (MQ=255)

                                 ggTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAacca                                                       <  1:8557742/93‑1 (MQ=255)

                                 ggTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAacca                                                       <  2:2418890/93‑1 (MQ=255)

                                 ggTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAacca                                                       >  2:9584688/1‑93 (MQ=255)

                                 ggTTGCCGTCCAGCGCCTGAATAATCAGACACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAacca                                                       <  1:1947562/93‑1 (MQ=255)

                                  gTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAaccac                                                      >  2:8868544/1‑93 (MQ=255)

                                        gTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAAt                                                <  2:1369626/93‑1 (MQ=255)

                                        gTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAAt                                                >  1:1919855/1‑93 (MQ=255)

                                           cAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGt                                             >  1:8992385/1‑93 (MQ=255)

                                            aGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTc                                            >  1:4667004/1‑93 (MQ=255)

                                             gcgcCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTca                                           >  2:4613511/1‑93 (MQ=255)

                                             gcgcCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTca                                           <  2:919925/93‑1 (MQ=255)

                                              cgcCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTcac                                          <  2:7675557/93‑1 (MQ=255)

                                              cgcCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTcac                                          <  2:8684794/93‑1 (MQ=255)

                                               gcCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTcacc                                         >  2:546832/1‑93 (MQ=255)

                                               gcCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTcacc                                         >  1:7301336/1‑93 (MQ=255)

                                                  tGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACcaca                                      >  1:8272003/1‑93 (MQ=255)

                                                  tGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACcaca                                      >  1:5141750/1‑93 (MQ=255)

                                                   gAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACaa                                     >  1:9073430/1‑93 (MQ=255)

                                                   gAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACaa                                     <  1:6031099/93‑1 (MQ=255)

                                                    aataatCAGATACCCCCCGAAGCAGCTCCAGAATCCAGCCACTGCGCCAAACTGGAATAGAGCCACGCCAACCACCGGAATGGTCACCACaaa                                    <  1:1971657/93‑1 (MQ=255)

                                                    aataatCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACaaa                                    <  2:9701133/93‑1 (MQ=255)

                                                    aataatCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACaaa                                    <  2:2943081/93‑1 (MQ=255)

                                                     ataatCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAAt                                   >  2:6328496/1‑93 (MQ=255)

                                                     ataatCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAAt                                   >  2:5070664/1‑93 (MQ=255)

                                                      taatCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATg                                  >  1:8206973/1‑93 (MQ=255)

                                                      aaatCAGATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATg                                  <  1:3407527/92‑1 (MQ=255)

                                                            gATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCaa                            <  2:437526/93‑1 (MQ=255)

                                                            gATACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCaa                            <  2:5131413/93‑1 (MQ=255)

                                                             aTACACCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAAt                           >  2:3208815/1‑93 (MQ=255)

                                                               acacCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGt                         >  1:1089216/1‑93 (MQ=255)

                                                               acacCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGt                         <  2:8802507/93‑1 (MQ=255)

                                                                 acCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTAc                       >  1:1562621/1‑93 (MQ=255)

                                                                 acCGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTAc                       <  2:1091222/93‑1 (MQ=255)

                                                                  ccGCGAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACg                      <  2:6930582/93‑1 (MQ=255)

                                                                   cgcgAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACgg                     >  1:3215737/1‑93 (MQ=255)

                                                                   cgcgAAGCAGCCCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACgg                     >  2:6765027/1‑93 (MQ=255)

                                                                     cgAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGaa                   <  1:6467892/93‑1 (MQ=255)

                                                                      gAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAAt                  <  1:3937962/93‑1 (MQ=255)

                                                                      gAAGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAAt                  <  2:1509988/93‑1 (MQ=255)

                                                                       aaGCAGCTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATa                 >  1:7402438/1‑93 (MQ=255)

                                                                        agcagcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATaa                <  2:9446138/93‑1 (MQ=255)

                                                                         gcagcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAg               <  2:3940680/93‑1 (MQ=255)

                                                                          cagcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGa              <  2:6729399/93‑1 (MQ=255)

                                                                          cagcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGa              <  1:1765242/93‑1 (MQ=255)

                                                                          cagcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGa              <  1:5255268/93‑1 (MQ=255)

                                                                           agcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGaa             <  1:9104759/93‑1 (MQ=255)

                                                                            gcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAAc            <  2:6123183/93‑1 (MQ=255)

                                                                            gcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAAc            >  2:2032605/1‑93 (MQ=255)

                                                                            gcTCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAAc            <  2:372777/93‑1 (MQ=255)

                                                                            acTCCACAATTCAGTACCTTCGCCAAACTGGAATAGCGCCACGCCAACCACCGGATTGGTCACCACAAATGCGCCAATGTACGGAATAAGAAc            <  2:9191424/92‑1 (MQ=255)

                                                                              tCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCg          >  1:1251800/1‑93 (MQ=255)

                                                                              tCCAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCg          >  2:4298496/1‑93 (MQ=255)

                                                                               ccAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCga         >  1:9853472/1‑93 (MQ=255)

                                                                                cAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCgag        >  2:2314643/1‑93 (MQ=255)

                                                                                cAGAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCgag        <  2:7198875/93‑1 (MQ=255)

                                                                                  gAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGaa      <  2:7469771/93‑1 (MQ=255)

                                                                                  gAATTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGaa      <  2:4560370/93‑1 (MQ=255)

                                                                                  aaaTTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCGAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGaa      <  2:3167426/92‑1 (MQ=255)

                                                                                   aaTTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTCCGGAATAAGAACCGAGAAg     <  1:6612272/93‑1 (MQ=255)

                                                                                   aaTTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAg     >  1:743456/1‑93 (MQ=255)

                                                                                    aTTCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGc    <  1:2378547/93‑1 (MQ=255)

                                                                                     ttCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGcc   >  2:3316816/1‑93 (MQ=255)

                                                                                     ttCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGcc   >  2:6368380/1‑93 (MQ=255)

                                                                                     atCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGcc   <  1:5447412/92‑1 (MQ=255)

                                                                                      tCAGCACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGCCg  >  2:6586746/1‑93 (MQ=255)

TAACCGCTTCGGAGAACAACACCGGCACTAACAGGTTGCCGTCCAGCGCCTGAATAATCAGATACACCGCGAAGCAGCTCCAGAATTCAGTACCTGCGCCAAACTGGAATAGCGCCACGCCAACCACCGGAATGGTCACCACAAATGCGCCAATGTACGGAATAAGAACCGAGAAGCCG  >  NC_000913/2612986‑2613164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`