Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,669,800 T→C C186R (TGT→CGT)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,669,8000TC99.3% 857.2 / ‑4.2 272C186R (TGT→CGT) hcaB2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (146/124);  total (147/125)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 288 total aligned reads displayed.

ACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCC  >  NC_000913/2669711‑2669889
                                                                                         |                                                                                         
  acCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGt                                                                                      >  1:7878808/1‑93 (MQ=255)
   ccGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGGCCGCGTGGTa                                                                                     >  2:4161587/1‑93 (MQ=255)
   ccGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTa                                                                                     <  2:7264943/93‑1 (MQ=255)
       cAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGc                                                                                 >  1:9989966/1‑93 (MQ=255)
        aGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGcc                                                                                >  2:5329250/1‑93 (MQ=255)
        aGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGcc                                                                                <  2:4477371/93‑1 (MQ=255)
         gTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCa                                                                               >  1:7296807/1‑93 (MQ=255)
         gTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCa                                                                               >  1:9551510/1‑93 (MQ=255)
         gTAAACATGCCGCAACCGGACTTATTCGCCAACCGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCa                                                                               <  2:74818/93‑1 (MQ=255)
          tAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAg                                                                              >  1:5385169/1‑93 (MQ=255)
           aaaCATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAgg                                                                             >  2:9765183/1‑92 (MQ=255)
               aTGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGAcc                                                                         >  1:9005287/1‑93 (MQ=255)
                tGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCt                                                                        <  2:6257788/93‑1 (MQ=255)
                   cGCAACCGGACTTCTTCGCCAACTGTCTTATGAACTGGCACCGAAAGTGCGGGTGAATTGCGTCGTCCCGCGTGGTATGGCCAGCGACCTgcg                                                                     <  2:292748/93‑1 (MQ=255)
                   cGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTgcg                                                                     >  2:1475211/1‑93 (MQ=255)
                     cAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGcgcg                                                                   >  1:9331664/1‑93 (MQ=255)
                      aaCCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGTCCCGCGTGGTATGGCCAGCGACCTGCGCgg                                                                  >  1:8705220/1‑93 (MQ=255)
                       aCCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGc                                                                 >  2:2519754/1‑93 (MQ=255)
                        ccGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGcc                                                                >  2:5685633/1‑93 (MQ=255)
                         cGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGccc                                                               >  2:4366163/1‑93 (MQ=255)
                          ggACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCca                                                              >  1:7717284/1‑93 (MQ=255)
                          ggACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCca                                                              >  1:6456708/1‑93 (MQ=255)
                           gACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCcac                                                             >  1:8311776/1‑93 (MQ=255)
                           gACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCcac                                                             >  1:1419832/1‑93 (MQ=255)
                             cTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAg                                                           <  1:2122508/93‑1 (MQ=255)
                              ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGTCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          >  1:6993433/1‑93 (MQ=255)
                              ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          <  1:1020430/93‑1 (MQ=255)
                              ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          <  2:6438925/93‑1 (MQ=255)
                              ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          >  2:8542672/1‑93 (MQ=255)
                              ttATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAgg                                                          >  1:4804876/1‑93 (MQ=255)
                               tATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgc                                                         >  1:6777420/1‑93 (MQ=255)
                                aTTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgcg                                                        >  1:3379383/1‑93 (MQ=255)
                                 ttCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGgcgc                                                       >  2:9015278/1‑93 (MQ=255)
                                 ttCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTAGGCCCGCGGGGTATGGCCAGCGACCTGCGCGGCCCACAGgcgc                                                       >  2:3236166/1‑93 (MQ=255)
                                   cGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTc                                                     <  1:1213557/93‑1 (MQ=255)
                                    gCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCg                                                    <  2:5079104/93‑1 (MQ=255)
                                     ccAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCgg                                                   <  2:360694/93‑1 (MQ=255)
                                     ccAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCgg                                                   >  1:2405283/1‑93 (MQ=255)
                                      cAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCggg                                                  <  1:4755747/93‑1 (MQ=255)
                                      cAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCggg                                                  >  2:9911115/1‑93 (MQ=255)
                                       aaCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGc                                                 <  2:868975/93‑1 (MQ=255)
                                       aaCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGc                                                 >  2:3093901/1‑93 (MQ=255)
                                       aaCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGc                                                 >  1:2591164/1‑93 (MQ=255)
                                        aCTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCa                                                >  2:7067351/1‑93 (MQ=255)
                                         cTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaa                                               >  1:8167843/1‑93 (MQ=255)
                                          tGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCaaa                                              >  2:4726797/1‑93 (MQ=255)
                                           ggCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAg                                             <  2:3393946/93‑1 (MQ=255)
                                            gCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAgg                                            >  1:7734289/1‑92 (MQ=255)
                                            gCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAgg                                            >  1:3047857/1‑92 (MQ=255)
                                            gCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGt                                            >  2:7922993/1‑93 (MQ=255)
                                              ttATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGa                                          >  1:1732105/1‑93 (MQ=255)
                                              ttATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGa                                          <  1:5333466/93‑1 (MQ=255)
                                              ttATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGa                                          <  1:10039199/93‑1 (MQ=255)
                                               tATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGaa                                         >  1:6073157/1‑93 (MQ=255)
                                               tATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTCCGCGGCCCACAGGCGCTCGGCCAAAGTGaa                                         >  2:8719003/1‑93 (MQ=255)
                                                aTGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGaaa                                        <  1:5361178/93‑1 (MQ=255)
                                                  gAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAAcc                                      <  1:1991856/93‑1 (MQ=255)
                                                  gAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAAcc                                      <  2:5861819/93‑1 (MQ=255)
                                                   aaCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGGATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCt                                     >  2:7726509/1‑93 (MQ=255)
                                                    aCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGGGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTc                                    >  2:6030766/1‑93 (MQ=255)
                                                    aCTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTc                                    >  1:8450215/1‑93 (MQ=255)
                                                        gCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATa                                >  1:3827851/1‑93 (MQ=255)
                                                         cACCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATaa                               >  1:8043337/1‑93 (MQ=255)
                                                          aCCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATaag                              >  2:3791251/1‑92 (MQ=255)
                                                          aCCGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAAt                              <  2:3940749/93‑1 (MQ=255)
                                                           ccGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATaa                               <  2:7652000/91‑1 (MQ=255)
                                                           ccGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATaa                               >  1:7652001/1‑91 (MQ=255)
                                                           ccGAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATg                             >  1:2502361/1‑93 (MQ=255)
                                                            cGAAAGTGCGGGTGATTGGCGTCGGCCCGCGTGGTATGGCCATCGACCTGCGCGGCCTACAGGCGCTCGTGCAAAGTGATACTTTGATAATGc                            >  2:9273373/1‑93 (MQ=255)
                                                             gAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCa                           <  1:9438236/93‑1 (MQ=255)
                                                             gAAAGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCa                           >  2:3356404/1‑93 (MQ=255)
                                                              aaaGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAg                          <  1:4162506/93‑1 (MQ=255)
                                                              aaaGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAg                          <  1:4400249/93‑1 (MQ=255)
                                                               aaGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGt                         <  1:7401133/93‑1 (MQ=255)
                                                                aGTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtc                        <  1:5694939/93‑1 (MQ=255)
                                                                 gTGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtct                       >  1:6340890/1‑93 (MQ=255)
                                                                  tGCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCTGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGtctc                      <  2:1169011/93‑1 (MQ=255)
                                                                   gCGGGTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTctct                     <  2:8274028/93‑1 (MQ=255)
                                                                    gcggTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTg                    <  1:3108365/91‑1 (MQ=255)
                                                                     gggTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGa                   <  1:860262/93‑1 (MQ=255)
                                                                     gggTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGa                   <  1:9687263/93‑1 (MQ=255)
                                                                     gggTGAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGa                   <  1:1165925/93‑1 (MQ=255)
                                                                         gAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGcc               >  1:3178581/1‑93 (MQ=255)
                                                                         gAATGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGCCAAAGTGAAACCTCGATAATGCAGTCTCTGACGcc               <  1:784958/93‑1 (MQ=255)
                                                                          aaTGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCg              <  1:7100984/93‑1 (MQ=255)
                                                                          aaTGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCg              <  1:930661/93‑1 (MQ=255)
                                                                          aaTGGCGTCGGCACGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCg              <  2:4215030/93‑1 (MQ=255)
                                                                            tGGGGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGga            <  1:3632522/93‑1 (MQ=255)
                                                                            tGGCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGga            >  1:6690690/1‑93 (MQ=255)
                                                                             ggCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgag           >  1:3743628/1‑93 (MQ=255)
                                                                             ggCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgag           >  2:2009027/1‑93 (MQ=255)
                                                                              gCGTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGgaga          <  2:1725878/93‑1 (MQ=255)
                                                                                gTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGcaa        >  1:2866160/1‑93 (MQ=255)
                                                                                gTCGGCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGaaa        >  2:8004297/1‑93 (MQ=255)
                                                                                  cGGCCCGCGTGGTATGTCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAAt      <  1:4506891/93‑1 (MQ=255)
                                                                                  cGGCCCGCGTGGTATGGCAAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAAt      <  2:1927113/93‑1 (MQ=255)
                                                                                   ggCCCGCGTGGTATGGCCAGTGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAAtt     >  2:3505543/1‑93 (MQ=255)
                                                                                   ggCCCGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAAtt     <  1:2333848/93‑1 (MQ=255)
                                                                                      ccGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTgcc  >  1:7602568/1‑93 (MQ=255)
                                                                                      ccGCGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTgcc  <  1:5991350/93‑1 (MQ=255)
                                                                                         |                                                                                         
ACACCGCCAGTAAACATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGGTATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAATTGCC  >  NC_000913/2669711‑2669889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑