BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,699,813	T→C	E226G (GAA→GGA)	recO ←	RecO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,699,813	0	T	C	99.7%	1017.5 / ‑5.1	315	E226G (GAA→GGA)	recO	RecO
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (183/131); total (183/132)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

Only 100 of 333 total aligned reads displayed.

GTTGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAG  >  NC_000913/2699723‑2699900

gTTGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTccc                                                                                       >  2:7852722/1‑93 (MQ=255)

 ttGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTcccc                                                                                      <  1:5870064/93‑1 (MQ=255)

 ttGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTcccc                                                                                      >  1:7653386/1‑93 (MQ=255)

  tGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCt                                                                                     >  1:2589229/1‑93 (MQ=255)

  tGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCt                                                                                     <  2:3596287/93‑1 (MQ=255)

   gACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTg                                                                                    >  2:6406278/1‑93 (MQ=255)

    aCGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGc                                                                                   <  1:6487234/93‑1 (MQ=255)

       ccTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAACCTGCCGGAACAGTCCCCTGctct                                                                                >  1:894057/1‑93 (MQ=255)

        cTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCtt                                                                               <  2:2233636/93‑1 (MQ=255)

        cTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCtt                                                                               <  2:2115506/93‑1 (MQ=255)

        cTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCA‑CGTTCGCTTAGGTCTAAACTGCCGGAACAGTCCCCTGCTCttt                                                                              >  1:1582161/1‑93 (MQ=255)

         tAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCttt                                                                              >  1:5588979/1‑93 (MQ=255)

         tAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCttt                                                                              >  2:7441481/1‑93 (MQ=255)

          aaCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTa                                                                             >  1:8658004/1‑93 (MQ=255)

           aCAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTaa                                                                            >  1:4943182/1‑93 (MQ=255)

                aaTTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGtt                                                                       <  2:8609750/93‑1 (MQ=255)

                aaTGCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGtt                                                                       >  2:9836795/1‑93 (MQ=255)

                 aTTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGttt                                                                      >  2:2976401/1‑93 (MQ=255)

                  ttCATCCATGACAATCCTCATCATTCATAATGGGTTTTCACCGTTCGCTTAGGAATAAACTGCCGGAACAGTAAACTGCTCTTTAAAGGATTa                                                                     >  2:8330432/1‑93 (MQ=255)

                  ttCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTa                                                                     >  1:998744/1‑93 (MQ=255)

                   tCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTAc                                                                    <  1:66312/93‑1 (MQ=255)

                    cAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTAcc                                                                   <  1:9636802/93‑1 (MQ=255)

                      gCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGc                                                                 >  1:6101091/1‑93 (MQ=255)

                      gCCATGACAATCCTCATCATTCATAATGTGTTTTCACAGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGc                                                                 >  1:7095069/1‑93 (MQ=255)

                       ccATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGcc                                                                >  1:5927089/1‑93 (MQ=255)

                         aTGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCaa                                                              <  1:381024/93‑1 (MQ=255)

                         aTGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCaa                                                              >  1:1758323/1‑93 (MQ=255)

                          tGACAATCCTCATCATTCATAGTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAg                                                             >  2:7548438/1‑93 (MQ=255)

                          tGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAg                                                             >  1:3183979/1‑93 (MQ=255)

                          tGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAg                                                             >  1:2078522/1‑93 (MQ=255)

                            aCAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGAt                                                           >  1:1246670/1‑93 (MQ=255)

                             cAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATa                                                          <  2:2878958/93‑1 (MQ=255)

                             cAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATa                                                          <  2:8757281/93‑1 (MQ=255)

                             cAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATa                                                          >  1:3447760/1‑93 (MQ=255)

                              aaTCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATAc                                                         >  2:2387970/1‑93 (MQ=255)

                              aaTCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATAc                                                         <  1:1141027/93‑1 (MQ=255)

                              aaTCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATAc                                                         <  1:7546013/93‑1 (MQ=255)

                               aTCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACg                                                        >  2:6875744/1‑93 (MQ=255)

                                 ccTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGc                                                      <  2:7040491/93‑1 (MQ=255)

                                 ccTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGc                                                      <  1:584222/93‑1 (MQ=255)

                                  cTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCt                                                     >  2:1505531/1‑93 (MQ=255)

                                  cTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCt                                                     >  1:2436549/1‑93 (MQ=255)

                                   tcatcaTTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCtt                                                    >  2:1766876/1‑93 (MQ=255)

                                   tcatcaTTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCtt                                                    <  1:6004373/93‑1 (MQ=255)

                                    catcatTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTa                                                   >  2:6247700/1‑93 (MQ=255)

                                      tcattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAg                                                 >  1:8034306/1‑93 (MQ=255)

                                       cattcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgc                                                >  2:3444916/1‑93 (MQ=255)

                                        attcatAATGTGTTTTTACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcg                                               >  2:8978187/1‑93 (MQ=255)

                                        attcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcg                                               >  2:2658684/1‑93 (MQ=255)

                                         ttcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcgc                                              >  2:6279584/1‑93 (MQ=255)

                                         ttcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcgc                                              >  2:122288/1‑93 (MQ=255)

                                         ttcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcgc                                              >  2:7645432/1‑93 (MQ=255)

                                         ttcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAgcgc                                              >  1:9160588/1‑93 (MQ=255)

                                          tcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGcc                                             >  2:821044/1‑93 (MQ=255)

                                          tcatAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGcc                                             >  2:4536015/1‑93 (MQ=255)

                                           catAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCa                                            >  1:4606317/1‑93 (MQ=255)

                                            atAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCAt                                           >  1:4423602/1‑93 (MQ=255)

                                              aaTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGc                                         <  2:5746665/93‑1 (MQ=255)

                                              aaTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGc                                         >  2:4386324/1‑93 (MQ=255)

                                              aaTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGc                                         <  2:5820140/93‑1 (MQ=255)

                                              aaTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGc                                         <  1:3690492/93‑1 (MQ=255)

                                               aTGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCg                                        >  2:1687203/1‑93 (MQ=255)

                                                tgtgTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCgg                                       >  1:10037688/1‑93 (MQ=255)

                                                      ttCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAg                                 <  1:5211297/93‑1 (MQ=255)

                                                      ttCACCGTTCGCTGAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAg                                 <  2:962754/93‑1 (MQ=255)

                                                        cACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCg                               >  1:2840439/1‑93 (MQ=255)

                                                        cACCGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCg                               >  2:5002821/1‑93 (MQ=255)

                                                          ccGTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGtt                             >  2:1188629/1‑93 (MQ=255)

                                                           cGTTCGCATAGGCATAAACTGCCAGAACAGTCCCCTGCTCTTTAAAGCTTTGCCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGttt                            <  1:7448937/93‑1 (MQ=255)

                                                            gTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTc                           >  2:7280038/1‑93 (MQ=255)

                                                            gTTCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTc                           >  2:5854418/1‑93 (MQ=255)

                                                              tCGCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTcgc                         >  1:6385423/1‑93 (MQ=255)

                                                                gCTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCgcgg                       <  2:9229778/93‑1 (MQ=255)

                                                                 cTTAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCgcggc                      >  2:9799379/1‑93 (MQ=255)

                                                                 cTTAGGCATAAACTGCCGGAACAGGCCCCTGCTCTTTAAAGGGTTACCGCCAAGATACGGCTTAAGCGCCAGGCGGGTAAAGCGTTTGgcggg                      >  1:2974972/1‑92 (MQ=255)

                                                                  ttAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCgcggcg                     >  2:7967651/1‑93 (MQ=255)

                                                                   tAGGCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGtcgc                    >  2:4173842/1‑93 (MQ=255)

                                                                     ggCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGgcgcgc                  >  2:196584/1‑93 (MQ=255)

                                                                     ggCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGgcgcgc                  <  1:622074/93‑1 (MQ=255)

                                                                     ggCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGgcgcgc                  <  2:654916/93‑1 (MQ=255)

                                                                      gCATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCa                 <  2:8544166/93‑1 (MQ=255)

                                                                       cATAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAg                <  1:9681597/93‑1 (MQ=255)

                                                                        aTAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgt               <  1:8266856/93‑1 (MQ=255)

                                                                        aTAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgt               >  1:9855535/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg              >  1:9281553/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg              >  2:5325688/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg              >  1:2127807/1‑93 (MQ=255)

                                                                         tAAACTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtg              >  2:1853658/1‑93 (MQ=255)

                                                                          aaaCTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgt             <  1:1955/93‑1 (MQ=255)

                                                                           aaCTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgtg            <  1:10002147/93‑1 (MQ=255)

                                                                           aaCTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgtg            <  1:5058998/93‑1 (MQ=255)

                                                                            aCTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgtgt           >  1:3865486/1‑93 (MQ=255)

                                                                            aCTGCCGGAACAGTCCCCTGCTCGTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAgtgtgt           <  2:5535438/93‑1 (MQ=255)

                                                                             cTGCCGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTc          <  1:4681071/93‑1 (MQ=255)

                                                                                ccGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGc       >  1:3390530/1‑93 (MQ=255)

                                                                                ccGGAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGc       <  2:3383106/93‑1 (MQ=255)

                                                                                  ggAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGCGTGTCTGCGt     >  1:6712613/1‑93 (MQ=255)

                                                                                   gAACAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTc    >  2:6623892/1‑93 (MQ=255)

                                                                                    aaCAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCCGCGCGCAGTGTGTCTGCGTCa   >  2:9346259/1‑93 (MQ=255)

                                                                                     aCAGTCCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAg  >  2:1384958/1‑93 (MQ=255)

GTTGACGCCTAACAGTAATTCAGCCATGACAATCCTCATCATTCATAATGTGTTTTCACCGTTCGCTTAGGCATAAACTGCCGGAACAGTTCCCTGCTCTTTAAAGGTTTACCGCCAAGATACGGCTTAAGCGCCATGCGGGTAAAGCGTTTCGCGGCGCGCAGTGTGTCTGCGTCAG  >  NC_000913/2699723‑2699900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`