BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,718,460	T→C	I163T (ATC→ACC)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,718,460	0	T	C	99.6%	849.4 / NA	264	I163T (ATC→ACC)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (137/126); minor base G (1/0); total (138/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCt                                                                                        <  2:3899707/93‑1 (MQ=255)

 gCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCt                                                                                        <  1:6081695/93‑1 (MQ=255)

  cAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTc                                                                                       >  2:253238/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:8174871/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  2:5889892/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:3437285/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:2850246/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  2:4726963/1‑93 (MQ=255)

    aGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGa                                                                                     >  1:10015527/1‑93 (MQ=255)

      gTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACt                                                                                   <  1:9926870/93‑1 (MQ=255)

       tCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTg                                                                                  >  1:5726703/1‑93 (MQ=255)

         tGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTggg                                                                                <  2:8089755/93‑1 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                               >  1:6338087/1‑93 (MQ=255)

          gAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgc                                                                               <  2:7620223/93‑1 (MQ=255)

           aaTGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGgcg                                                                              >  1:6836002/1‑93 (MQ=255)

             tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCa                                                                            >  1:8771705/1‑93 (MQ=255)

             tGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCa                                                                            <  2:4281432/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  2:1408842/93‑1 (MQ=255)

               ccAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAAc                                                                          <  1:7563616/93‑1 (MQ=255)

                cAGCCTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACa                                                                         <  1:228022/93‑1 (MQ=255)

                  gCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGc                                                                       <  1:7847128/93‑1 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                    >  2:7056394/1‑93 (MQ=255)

                     tttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTa                                                                    >  2:7382896/1‑93 (MQ=255)

                      ttCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTaa                                                                   <  1:9406527/93‑1 (MQ=255)

                       tCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAg                                                                  >  1:9099742/1‑93 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTTGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  1:6572908/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  2:6563977/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  2:518592/93‑1 (MQ=255)

                        cGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGa                                                                 <  2:830864/93‑1 (MQ=255)

                          ccTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCTTCTCCACCGCCTCCCTCGACTGGGCGCAACAGCGTAAGATg                                                               <  2:4615441/93‑1 (MQ=255)

                          ccTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATg                                                               >  1:5992345/1‑93 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           <  1:3178738/93‑1 (MQ=255)

                              tgCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCt                                                           >  2:5659907/1‑93 (MQ=255)

                                ccGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCAGCCTCGACTGGGCGCAACAGCGTAAGATGGGCttt                                                         >  1:6214327/1‑93 (MQ=255)

                                 cGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCtttt                                                        >  2:1660210/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       >  2:3690671/1‑93 (MQ=255)

                                  gATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTc                                                       <  1:4538661/93‑1 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGCGCTTTTCCt                                                     >  1:458189/1‑93 (MQ=255)

                                    ttAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGGCTCCAACACCACCCTCGTCTGGGCTCAACAGCGTAAGATTTGCTTTTCCt                                                     >  1:7524679/1‑93 (MQ=255)

                                     tAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                    >  2:9690314/1‑93 (MQ=255)

                                     tAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTa                                                    >  2:1136327/1‑93 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGTCTTTTCCTat                                                   >  1:7673985/1‑92 (MQ=255)

                                      aaaCGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAc                                                   >  1:3019887/1‑93 (MQ=255)

                                         cgcgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACttt                                                <  2:2319504/93‑1 (MQ=255)

                                           cgacAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAt                                              <  2:6479196/90‑1 (MQ=255)

                                           cgGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAt                                              <  1:6418854/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  2:1925515/93‑1 (MQ=255)

                                            ggCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTAtt                                             <  1:2501278/93‑1 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGGGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           >  1:8370758/1‑93 (MQ=255)

                                              cAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgc                                           <  2:7589576/93‑1 (MQ=255)

                                               aaGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcg                                          >  1:231778/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         >  2:1826551/1‑93 (MQ=255)

                                                aGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTgcgc                                         <  2:627673/93‑1 (MQ=255)

                                                  cTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTc                                       <  1:8244638/93‑1 (MQ=255)

                                                   tGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCg                                      >  1:6970277/1‑93 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                    <  1:6707002/93‑1 (MQ=255)

                                                     gCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGc                                    <  1:2684527/93‑1 (MQ=255)

                                                       gTTTATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGa                                  >  1:1134499/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGaa                                 >  1:5970191/1‑92 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:1675759/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:240297/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  2:8645936/1‑93 (MQ=255)

                                                        tttATTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGAc                                 >  1:7981021/1‑93 (MQ=255)

                                                           aTTTCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGc                              >  2:2946905/1‑93 (MQ=255)

                                                            tttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGcc                             <  1:5317038/93‑1 (MQ=255)

                                                             ttCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGccg                            >  1:4837229/1‑92 (MQ=255)

                                                              tCGCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTg                           >  2:6006166/1‑93 (MQ=255)

                                                                gCAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGa                         <  2:9046940/93‑1 (MQ=255)

                                                                 cAATCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGTGCTCGGCGACAGCCTGGat                        >  1:3888225/1‑93 (MQ=255)

                                                                  aaTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGata                       <  1:9787287/93‑1 (MQ=255)

                                                                   aTCGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGatat                      <  2:7902445/93‑1 (MQ=255)

                                                                     cGGCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCg                    <  1:6760282/93‑1 (MQ=255)

                                                                      ggCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGa                   >  1:7222208/1‑93 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGAc                  >  1:9585543/1‑93 (MQ=255)

                                                                       gCTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGCATATCGAc                  >  1:4022225/1‑93 (MQ=255)

                                                                        cTGCCGTCTCCAACACCTCCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                 <  1:1496074/93‑1 (MQ=255)

                                                                        cTGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACg                 <  2:8028102/93‑1 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTAGTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                <  2:9896196/93‑1 (MQ=255)

                                                                         tGCCGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGt                >  2:7499699/1‑93 (MQ=255)

                                                                            cGTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtga             >  2:4198260/1‑93 (MQ=255)

                                                                             gTCTCCAACCCCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            <  2:9551372/93‑1 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            >  1:9350518/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            >  2:4388641/1‑93 (MQ=255)

                                                                             gTCTCCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgat            <  2:6647421/93‑1 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg           >  2:6227477/1‑93 (MQ=255)

                                                                              tctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatg           >  1:2101323/1‑93 (MQ=255)

                                                                               ctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatga          <  1:5370957/93‑1 (MQ=255)

                                                                               ctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatga          <  1:4197314/93‑1 (MQ=255)

                                                                               ctcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTtgatga          <  1:8519524/93‑1 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCTCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  2:7220912/93‑1 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGTTCGGCGAAACCCTGGCTATCGACGTtgacgat         >  1:1252701/1‑92 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         >  2:2060841/1‑93 (MQ=255)

                                                                                tcCAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGaa         <  2:4002782/93‑1 (MQ=255)

                                                                                 ccAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAt        <  2:681717/93‑1 (MQ=255)

                                                                                  cAACACCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAAtt       >  2:2191475/1‑93 (MQ=255)

                                                                                    acacCACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGc     >  1:8008162/1‑93 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    <  2:350838/93‑1 (MQ=255)

                                                                                     caccacCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCt    >  2:6118735/1‑93 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  >  1:7075856/1‑93 (MQ=255)

                                                                                       ccACCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTg  >  2:1318817/1‑93 (MQ=255)

GGCAAGGTCTGAATGCCAGCTTTTCGCCTGTGCCGATTAAACGCGGCAAGCTGGCGTTTATTTCGCAATCGGCTGCCGTCTCCAACACCATCCTCGACTGGGCGCAACAGCGTAAGATGGGCTTTTCCTACTTTATTGCGCTCGGCGACAGCCTGGATATCGACGTTGATGAATTGCTTG  >  NC_000913/2718370‑2718549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`