BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	317,326	T→C	Y76C (TAC→TGC)	ykgB ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	317,326	0	T	C	99.6%	1578.8 / ‑3.7	482	Y76C (TAC→TGC)	ykgB	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (276/202); total (279/203)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01

Only 100 of 492 total aligned reads displayed.

AGCAGAGCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGTATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGCGACGAATGGTGTA  >  NC_000913/317236‑317415

 gcagAGCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCAtt                                                                                        >  1:8457857/1‑93 (MQ=255)

 gcagAGCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCAtt                                                                                        >  2:6431066/1‑93 (MQ=255)

   agagCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCg                                                                                      >  2:8020682/1‑93 (MQ=255)

     agCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGcc                                                                                    <  2:4734983/93‑1 (MQ=255)

      gCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCt                                                                                   <  2:9104645/93‑1 (MQ=255)

      gCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCt                                                                                   >  1:2746261/1‑93 (MQ=255)

       cAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCtt                                                                                  <  1:726185/93‑1 (MQ=255)

          taatCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGt                                                                               >  2:1832821/1‑93 (MQ=255)

          taatCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGt                                                                               >  1:5065209/1‑93 (MQ=255)

          taatCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGt                                                                               >  1:3124732/1‑93 (MQ=255)

             tCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTACATCTGGTTTGCATTCGCCTTCGTGAg                                                                            >  2:7386572/1‑93 (MQ=255)

              cACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGt                                                                           <  2:5550100/93‑1 (MQ=255)

               cccTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTc                                                                          <  2:6872363/92‑1 (MQ=255)

                ccTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCa                                                                         <  1:8234677/93‑1 (MQ=255)

                ccTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATGATTGGCCGTTTGCCACGCCCTTGGTTCTGGTTTGTATTCGCCTTCGTGatgca                                                                         >  1:4120343/1‑89 (MQ=255)

                 cTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAg                                                                        >  2:1856904/1‑93 (MQ=255)

                 cTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAg                                                                        <  1:8844763/93‑1 (MQ=255)

                  tCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGa                                                                       <  1:6113890/93‑1 (MQ=255)

                  cccACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGa                                                                       <  2:7875049/92‑1 (MQ=255)

                  accACGACGCCAAGACCGTTGGAAAACCCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGa                                                                       <  2:2130171/92‑1 (MQ=255)

                   ccACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGAt                                                                      <  2:6341027/93‑1 (MQ=255)

                   ccACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGAt                                                                      <  2:5690479/93‑1 (MQ=255)

                   ccACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGAt                                                                      >  2:508874/1‑93 (MQ=255)

                     acgacgCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATAc                                                                    >  1:2156637/1‑93 (MQ=255)

                      cgacgCGAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACt                                                                   <  2:5189817/93‑1 (MQ=255)

                      cgacgCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACt                                                                   <  2:2584670/93‑1 (MQ=255)

                       gacgCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTg                                                                  <  2:1059844/93‑1 (MQ=255)

                       gacgCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTg                                                                  >  2:8502210/1‑93 (MQ=255)

                         cgCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCAGGCCCTTGCTTCTGGTTTTCATTCGCCTTGGTGAGGAAGATAcgttt                                                                >  1:3628041/1‑89 (MQ=255)

                          gCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGttt                                                               >  1:4871187/1‑93 (MQ=255)

                           ccAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTta                                                              >  2:9222057/1‑93 (MQ=255)

                            cAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTtat                                                             >  1:1351503/1‑93 (MQ=255)

                              aGACCGTTGGAAAAACCATAGGGATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAg                                                           >  1:1218985/1‑93 (MQ=255)

                               gACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGt                                                          >  1:735205/1‑93 (MQ=255)

                               gACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGt                                                          <  2:7048588/93‑1 (MQ=255)

                                 ccGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCt                                                        >  1:10003604/1‑93 (MQ=255)

                                  cGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCtt                                                       >  2:8627747/1‑93 (MQ=255)

                                   gTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTc                                                      >  2:9488344/1‑93 (MQ=255)

                                   gTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTATATAGTCTTc                                                      >  1:1139652/1‑93 (MQ=255)

                                    ttGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCCTTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTcc                                                     >  1:8550826/1‑93 (MQ=255)

                                     tGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCg                                                    <  1:177135/93‑1 (MQ=255)

                                     tGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCg                                                    >  1:9486510/1‑93 (MQ=255)

                                     tGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCg                                                    >  2:195282/1‑93 (MQ=255)

                                      ggAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCgg                                                   >  2:6681903/1‑93 (MQ=255)

                                       gAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCggg                                                  <  1:7947876/93‑1 (MQ=255)

                                       gAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCggg                                                  >  1:153187/1‑93 (MQ=255)

                                       gAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCggg                                                  >  1:3958808/1‑93 (MQ=255)

                                       gAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCggg                                                  <  2:1333506/93‑1 (MQ=255)

                                        aaaaaCCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGgt                                                 >  2:9035340/1‑93 (MQ=255)

                                        aaaaaCCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGgt                                                 >  1:3513997/1‑93 (MQ=255)

                                            aCCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTc                                             <  2:8373492/93‑1 (MQ=255)

                                             ccATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCa                                            <  2:2777418/93‑1 (MQ=255)

                                               aTAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATa                                          >  2:9763681/1‑93 (MQ=255)

                                                tAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATaa                                         >  1:5691032/1‑93 (MQ=255)

                                                tAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATaa                                         >  2:6563173/1‑93 (MQ=255)

                                                  ggaattattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATaaaa                                       >  1:4278767/4‑93 (MQ=255)

                                                   gTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATaaaaa                                      >  1:2364965/1‑92 (MQ=255)

                                                   gTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAg                                      <  2:5848326/93‑1 (MQ=255)

                                                    tattatTGGCCGTTTGCCATGACCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGa                                     >  2:8939259/1‑93 (MQ=255)

                                                     attattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGaa                                    >  2:3685484/1‑93 (MQ=255)

                                                     attattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGaa                                    <  2:2904013/93‑1 (MQ=255)

                                                      ttattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAACAGAAc                                   >  1:6661548/1‑93 (MQ=255)

                                                      ttattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAAc                                   >  2:1212371/1‑93 (MQ=255)

                                                      ttattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAAc                                   <  2:2687344/93‑1 (MQ=255)

                                                      ttattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAAc                                   >  1:2303591/1‑93 (MQ=255)

                                                      ttattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGAATTAGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGCGTTCATAAAAGAAc                                   >  2:3747162/1‑93 (MQ=255)

                                                       tattGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACg                                  >  1:9816382/1‑93 (MQ=255)

                                                        attGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGa                                 <  1:5273314/93‑1 (MQ=255)

                                                         ttGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGAc                                <  1:9311344/93‑1 (MQ=255)

                                                          tGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACa                               >  1:587634/1‑93 (MQ=255)

                                                           tgCCCTTTGCCCTTCCCTTGCTTCTGGTTTCCATTCCCCTTCGTGAGTCAGATCCTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACCACAt                              <  1:7160074/92‑1 (MQ=255)

                                                           ggCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACAt                              >  2:4032952/1‑93 (MQ=255)

                                                            gCCGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACAtt                             <  1:7547788/93‑1 (MQ=255)

                                                             ccGTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCAGAAAAGAACGACATTa                            <  2:1731330/93‑1 (MQ=255)

                                                              cGTTTGCTATGCCCTTGCGTCTGTTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAg                           <  1:8667071/93‑1 (MQ=255)

                                                               gTTTGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGt                          >  1:7761836/1‑93 (MQ=255)

                                                                tttGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATGAGTg                         >  2:6083701/1‑93 (MQ=255)

                                                                 ttGCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTgg                        >  2:3021436/1‑93 (MQ=255)

                                                                   gCCATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGAc                      <  2:492408/93‑1 (MQ=255)

                                                                     cATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTg                    >  1:5449209/1‑93 (MQ=255)

                                                                     cATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTg                    >  1:1958383/1‑93 (MQ=255)

                                                                     cATGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGCGTTCATAAAAGAACGACATTAGTGGACTg                    >  1:5204194/1‑93 (MQ=255)

                                                                      aTGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGt                   >  1:6378415/1‑93 (MQ=255)

                                                                      aTGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGt                   >  1:3757168/1‑93 (MQ=255)

                                                                      aTGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGt                   >  2:7676340/1‑93 (MQ=255)

                                                                       tGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGtt                  >  2:3413802/1‑93 (MQ=255)

                                                                       tGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGtt                  >  1:4612034/1‑93 (MQ=255)

                                                                       tGCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGtt                  >  2:2301563/1‑93 (MQ=255)

                                                                        gCCCTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGttt                 <  2:4491068/93‑1 (MQ=255)

                                                                           cTTGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcg              >  1:9035884/1‑93 (MQ=255)

                                                                            ttGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcga             <  1:7434680/93‑1 (MQ=255)

                                                                            ttGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcga             <  2:10002509/93‑1 (MQ=255)

                                                                            ttGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcga             <  1:2936355/93‑1 (MQ=255)

                                                                            ttGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcga             >  1:6857815/1‑93 (MQ=255)

                                                                            ttGCTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcga             >  1:388362/1‑93 (MQ=255)

                                                                             tgctgcTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcgac            <  2:321547/93‑1 (MQ=255)

                                                                               cTTCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGcgacga          <  1:3377221/93‑1 (MQ=255)

                                                                                ttCTGGTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGCGACGaa         <  2:1495313/93‑1 (MQ=255)

                                                                                 tCTGGTTTGCATTCGGCTTCGTGAGTCCGATACTGTTTATAGTCTTCCGGGTGTTCATCAAAGAACGACATTAGTGGACTGTTTGCGACGAAt        >  2:4195486/1‑93 (MQ=255)

                                                                                    ggTTTGCATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGCGACGAATGgt     >  2:6639308/1‑93 (MQ=255)

AGCAGAGCAATAATCACCTCCACGACGCCAAGACCGTTGGAAAAACCATAGGTATTATTGGCCGTTTGCCATGCCCTTGCTTCTGGTTTGTATTCGCCTTCGTGAGTCAGATACTGTTTATAGTCTTCCGGGTGTTCATAAAAGAACGACATTAGTGGACTGTTTGCGACGAATGGTGTA  >  NC_000913/317236‑317415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`