BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,772,146	C→T	L270F (CTT→TTT)	yfjW →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,772,146	0	C	T	93.6%	1570.2 / 47.0	481	L270F (CTT→TTT)	yfjW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (16/15); new base T (211/239); total (227/254)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.82e-01

Only 100 of 497 total aligned reads displayed.

ATATATAATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAATCCGCTGA  >  NC_000913/2772056‑2772237

atatatAATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGttt                                                                                           <  1:983802/93‑1 (MQ=255)

atatatAATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGttt                                                                                           >  1:6820568/1‑93 (MQ=255)

atatatAATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGttt                                                                                           >  1:8148703/1‑93 (MQ=255)

 tatataATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTg                                                                                          >  1:7937198/1‑93 (MQ=255)

  atataATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGa                                                                                         <  2:4360615/93‑1 (MQ=255)

  atataATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGa                                                                                         <  2:9038543/93‑1 (MQ=255)

   tataATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGAt                                                                                        <  2:7672703/93‑1 (MQ=255)

    ataATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATg                                                                                       >  2:2830982/1‑93 (MQ=255)

    ataATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATg                                                                                       <  2:1628453/93‑1 (MQ=255)

     taATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGc                                                                                      <  2:8120843/93‑1 (MQ=255)

     taATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGc                                                                                      >  1:5948560/1‑93 (MQ=255)

     taATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGc                                                                                      >  1:4729682/1‑93 (MQ=255)

      aaTGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCt                                                                                     >  2:2549465/1‑93 (MQ=255)

       aTGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTa                                                                                    <  2:7087537/93‑1 (MQ=255)

        tGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTaa                                                                                   <  1:6642069/93‑1 (MQ=255)

         gATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTaaa                                                                                  <  1:156812/93‑1 (MQ=255)

         gATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTaaa                                                                                  <  2:3416036/93‑1 (MQ=255)

         gATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTaaa                                                                                  >  2:4016924/1‑93 (MQ=255)

          aTACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTaaaa                                                                                 >  1:5859606/1‑93 (MQ=255)

           tACACAGTGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTaaaaa                                                                                <  1:4829520/93‑1 (MQ=255)

              acaGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATc                                                                             >  1:4483965/1‑93 (MQ=255)

               caGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCt                                                                            >  2:5320331/1‑93 (MQ=255)

                 gggTTTAAGTGGTGCTGATATTTATATTTTCTCCGGTTGTTATGCCCTTCGAGAGTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCttt                                                                          >  1:142977/1‑93 (MQ=255)

                 gggTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCttt                                                                          >  1:6968969/1‑93 (MQ=255)

                  ggTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCtttt                                                                         <  2:7461833/93‑1 (MQ=255)

                   gTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTc                                                                        <  2:9532379/93‑1 (MQ=255)

                   gTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTc                                                                        <  1:3915516/93‑1 (MQ=255)

                    tttAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCt                                                                       <  2:1006328/93‑1 (MQ=255)

                    tttAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCt                                                                       >  2:6307848/1‑93 (MQ=255)

                    tttAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCt                                                                       <  1:198712/93‑1 (MQ=255)

                     ttAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTg                                                                      >  1:827956/1‑93 (MQ=255)

                      tAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGa                                                                     <  1:3279374/93‑1 (MQ=255)

                       aaGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGaa                                                                    <  2:3871854/93‑1 (MQ=255)

                       aaGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGaa                                                                    >  2:9136904/1‑93 (MQ=255)

                        aGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAAt                                                                   >  2:8267612/1‑93 (MQ=255)

                           ggtgCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTa                                                                >  2:8793166/1‑93 (MQ=255)

                            gtgCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTGCTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaa                                                               >  1:1839275/1‑93 (MQ=255)

                            gtgCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaa                                                               <  1:7358484/93‑1 (MQ=255)

                             tgCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaaa                                                              <  1:7820538/93‑1 (MQ=255)

                             tgCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaaa                                                              >  2:3318322/1‑93 (MQ=255)

                               cTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaaaaa                                                            <  2:5685189/93‑1 (MQ=255)

                               cTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaaaaa                                                            >  2:4621532/1‑93 (MQ=255)

                               cTGATATTTATATTATCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaaaaa                                                            <  1:1625860/93‑1 (MQ=255)

                                tGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTaaaaaa                                                           <  2:9613391/93‑1 (MQ=255)

                                 gATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAg                                                          >  2:6767545/1‑93 (MQ=255)

                                  atatTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGc                                                         <  2:4670113/93‑1 (MQ=255)

                                  atatTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGc                                                         >  1:878744/1‑93 (MQ=255)

                                   tatTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCt                                                        <  2:8697866/93‑1 (MQ=255)

                                    atTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTa                                                       <  1:9403401/93‑1 (MQ=255)

                                    atTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTa                                                       <  1:3453818/93‑1 (MQ=255)

                                    atTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTa                                                       <  2:7957682/93‑1 (MQ=255)

                                    atTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTa                                                       <  1:1004818/93‑1 (MQ=255)

                                    atTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCTGAATGT‑AAAAAGCTAg                                                      <  2:1557862/93‑1 (MQ=255)

                                     tttATATTGTCTCCTGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAg                                                      <  1:7665861/93‑1 (MQ=255)

                                     tttATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAACAAAGCTAg                                                      >  1:4102050/1‑93 (MQ=255)

                                     tttATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAg                                                      <  1:3504582/93‑1 (MQ=255)

                                     tttATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAg                                                      >  2:1427439/1‑93 (MQ=255)

                                      ttATATTGTCTCCGGTTGTTATGGCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGa                                                     <  2:5499556/93‑1 (MQ=255)

                                       tataTTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGaa                                                    <  2:7441557/93‑1 (MQ=255)

                                        atatTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGaaa                                                   >  1:2448080/1‑93 (MQ=255)

                                         tatTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAAt                                                  >  2:2054219/1‑93 (MQ=255)

                                             gTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATcaca                                              <  1:6187536/93‑1 (MQ=255)

                                             gTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATcaca                                              >  2:9369192/1‑93 (MQ=255)

                                              tctcCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATcacac                                             >  1:8522192/1‑93 (MQ=255)

                                              tctcCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATcacac                                             <  2:7257764/93‑1 (MQ=255)

                                              tctcCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATcacac                                             >  1:4956454/1‑93 (MQ=255)

                                                tcCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAg                                           >  1:2759699/1‑93 (MQ=255)

                                                 ccGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGa                                          >  2:7774607/1‑93 (MQ=255)

                                                   ggTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGaaa                                        <  1:989770/93‑1 (MQ=255)

                                                       gttATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTc                                    >  1:7197821/1‑93 (MQ=255)

                                                        ttATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTcc                                   >  1:5703676/1‑93 (MQ=255)

                                                        ttATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTcc                                   <  2:6670753/93‑1 (MQ=255)

                                                         tATGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCa                                  <  2:2313092/93‑1 (MQ=255)

                                                          aTGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCaa                                 <  1:5147183/93‑1 (MQ=255)

                                                          aTGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCaa                                 <  1:258971/93‑1 (MQ=255)

                                                          aTGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCaa                                 <  2:1924195/93‑1 (MQ=255)

                                                           tGCCCTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAat                                >  1:171793/1‑93 (MQ=255)

                                                             cccTTCTAGATTTACTTCGCAATGTACCGTTTCATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAatga                              <  1:8745749/93‑1 (MQ=255)

                                                             cccTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAatga                              <  1:9449403/93‑1 (MQ=255)

                                                             cccTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAatga                              <  2:3646376/93‑1 (MQ=255)

                                                               cTTCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAatgatg                            <  1:5547821/93‑1 (MQ=255)

                                                                ttCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATgg                           <  2:2973472/93‑1 (MQ=255)

                                                                ttCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATgg                           <  1:7470128/93‑1 (MQ=255)

                                                                 tCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATggg                          >  2:697670/1‑93 (MQ=255)

                                                                 tCCAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATggg                          <  2:5840366/93‑1 (MQ=255)

                                                                  ccAGATTTACTTCGCGATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATgggg                         >  2:6426434/1‑93 (MQ=255)

                                                                  ccAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATgggg                         >  1:2993645/1‑93 (MQ=255)

                                                                  ccAGATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATgggg                         >  2:5132729/1‑93 (MQ=255)

                                                                     gATTTACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTc                      >  2:6127038/1‑93 (MQ=255)

                                                                         tACTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATAc                  >  1:4371393/1‑93 (MQ=255)

                                                                          aCTTCGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACa                 >  2:7534636/1‑93 (MQ=255)

                                                                              cGCAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGt             <  2:8871374/93‑1 (MQ=255)

                                                                                cAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGa           <  2:4750434/93‑1 (MQ=255)

                                                                                cAATGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGa           >  2:8414130/1‑93 (MQ=255)

                                                                                 aaTGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGaa          <  1:9019312/93‑1 (MQ=255)

                                                                                 aaTGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGaa          <  1:9553323/93‑1 (MQ=255)

                                                                                  aTGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAAt         >  1:6387661/1‑93 (MQ=255)

                                                                                   tGTACCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAATc        >  2:4964767/1‑93 (MQ=255)

                                                                                      aCCGTTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAATCCGc     >  1:123143/1‑93 (MQ=255)

                                                                                         gCTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAATCCGCtga  >  2:9901278/1‑93 (MQ=255)

ATATATAATGATACACAGGGTTTAAGTGGTGCTGATATTTATATTGTCTCCGGTTGTTATGCCCTTCCAGATTTACTTCGCAATGTACCGCTTGATGCTAAAAAATCTTTTCTGAATGTAAAAAAGCTAGAAATCACACAGAAACTTCCAATGATGGGGTTCATACAAGGTGAATCCGCTGA  >  NC_000913/2772056‑2772237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`