BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,877,392	T→C	E135G (GAG→GGG)	b2755 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,877,392	0	T	C	99.7%	1115.8 / ‑5.5	346	E135G (GAG→GGG)	b2755	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (156/187); total (157/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 366 total aligned reads displayed.

TAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGCA  >  NC_000913/2877302‑2877481

  gCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTAc                                                                                       <  2:8678860/93‑1 (MQ=255)

     aCGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGa                                                                                    >  1:774631/1‑93 (MQ=255)

     aCGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGa                                                                                    >  1:7313726/1‑93 (MQ=255)

     aCGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGa                                                                                    <  1:2921729/93‑1 (MQ=255)

      cGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAg                                                                                   <  1:1720640/93‑1 (MQ=255)

       gTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAgc                                                                                  >  2:8420945/1‑93 (MQ=255)

       gTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTAAGTTGCCTTACGGAgc                                                                                  >  2:2014082/1‑93 (MQ=255)

        tccattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAgcg                                                                                 >  2:7130278/1‑93 (MQ=255)

        tccattccatGTCACGCCGTATTGATTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAgcg                                                                                 >  2:5389283/1‑93 (MQ=255)

          cattccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGcc                                                                               <  1:5038924/93‑1 (MQ=255)

           attccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACATCTGAGTTGCCCTACGGAGCGCCg                                                                              <  2:6719660/93‑1 (MQ=255)

             tccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCggg                                                                            >  2:7420906/1‑93 (MQ=255)

              ccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGc                                                                           >  1:9538316/1‑93 (MQ=255)

              ccatGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGc                                                                           <  1:7584928/93‑1 (MQ=255)

               catGTCCCGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCa                                                                          <  1:7511969/93‑1 (MQ=255)

               catGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCa                                                                          <  1:9311952/93‑1 (MQ=255)

               catGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCa                                                                          >  1:4806612/1‑93 (MQ=255)

               catGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCa                                                                          <  1:6443817/93‑1 (MQ=255)

                 tGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAgg                                                                        >  2:3013137/1‑93 (MQ=255)

                 tGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAgg                                                                        <  1:2300384/93‑1 (MQ=255)

                 tGTCACGCCGTATTGCTTCGCCAGAAGTGCCTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAgg                                                                        <  2:1720981/93‑1 (MQ=255)

                   tCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGgcg                                                                      <  2:776787/93‑1 (MQ=255)

                    cACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGgcgc                                                                     <  1:7111083/93‑1 (MQ=255)

                     aCGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCa                                                                    <  1:5282198/93‑1 (MQ=255)

                      cGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGCTGCAATACGGAGCGCCGGGCAGGCGCAg                                                                   >  2:5302159/1‑93 (MQ=255)

                       gCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAgg                                                                  <  2:8010350/93‑1 (MQ=255)

                       gCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAgg                                                                  <  1:8069976/93‑1 (MQ=255)

                        ccGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGt                                                                 >  1:7025472/1‑93 (MQ=255)

                         cGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGtt                                                                <  1:360834/93‑1 (MQ=255)

                         cGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGtt                                                                >  1:1027243/1‑93 (MQ=255)

                          gTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtc                                                               >  2:8584773/1‑93 (MQ=255)

                           tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                              >  2:3487853/1‑93 (MQ=255)

                           tATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                              >  1:4196265/1‑93 (MQ=255)

                           gaTTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTtct                                                              <  1:1479806/92‑1 (MQ=255)

                             ttGCTTCGCCAGAAGTGCGTAGGTTGCCCGCCCGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTcc                                                            <  1:3393630/93‑1 (MQ=255)

                             ttGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTcc                                                            <  2:9733566/93‑1 (MQ=255)

                                cTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCaaa                                                         >  1:433908/1‑93 (MQ=255)

                                 ttCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                        <  1:6590849/93‑1 (MQ=255)

                                 ttCGCCAGAAGAGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAc                                                        >  2:1370972/1‑93 (MQ=255)

                                  tCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAAcc                                                       <  2:3118085/93‑1 (MQ=255)

                                    gCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGa                                                     >  2:5081268/1‑93 (MQ=255)

                                     ccAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGaa                                                    >  1:3759371/1‑93 (MQ=255)

                                      cAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                   <  2:6394068/93‑1 (MQ=255)

                                      cAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                   >  2:7827861/1‑93 (MQ=255)

                                      cAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAg                                                   >  2:2085485/1‑93 (MQ=255)

                                       aGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGt                                                  >  2:2800511/1‑93 (MQ=255)

                                       aGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGt                                                  >  1:8930958/1‑93 (MQ=255)

                                       aGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGt                                                  >  1:4615319/1‑93 (MQ=255)

                                        gAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGtt                                                 >  1:1911226/1‑93 (MQ=255)

                                           gTGCGTAGGTTGCTCTCACGCGACTGCCTTCTATACCTCTGAGTGGCGCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCaa                                              <  2:3993517/93‑1 (MQ=255)

                                           gTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCaa                                              <  2:9255986/93‑1 (MQ=255)

                                           gTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCaa                                              <  2:8391643/93‑1 (MQ=255)

                                              cGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACa                                           <  2:5828759/93‑1 (MQ=255)

                                               gTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCATGCGCAGGTTCTCCAAACCGAAGTTCAAACAt                                          >  1:441579/1‑93 (MQ=255)

                                               gTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAt                                          >  1:8732043/1‑93 (MQ=255)

                                                tAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtt                                         <  2:1818979/93‑1 (MQ=255)

                                                tAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtt                                         <  2:4169779/93‑1 (MQ=255)

                                                  ggTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACAtttt                                       <  1:4105674/93‑1 (MQ=255)

                                                   gTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTta                                      >  2:3333766/1‑93 (MQ=255)

                                                     tGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacg                                    <  1:9694800/93‑1 (MQ=255)

                                                     tGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacg                                    <  1:1348683/93‑1 (MQ=255)

                                                      gCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgt                                   <  2:5551079/93‑1 (MQ=255)

                                                       cccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgta                                  <  2:1230852/93‑1 (MQ=255)

                                                       cccGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgta                                  <  1:6081887/93‑1 (MQ=255)

                                                         cGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgtacg                                <  1:3961815/93‑1 (MQ=255)

                                                         cGCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTtacgtacg                                >  1:54457/1‑93 (MQ=255)

                                                          gCACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGa                               <  1:8484538/93‑1 (MQ=255)

                                                           cACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGAc                              <  1:7940688/93‑1 (MQ=255)

                                                           cACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGAc                              <  2:4514249/93‑1 (MQ=255)

                                                           cACGCGACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGAc                              <  1:6370327/93‑1 (MQ=255)

                                                              gcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCtt                           >  2:3653930/1‑93 (MQ=255)

                                                              gcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCtt                           >  2:2197183/1‑93 (MQ=255)

                                                              gcgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCtt                           >  1:7669563/1‑93 (MQ=255)

                                                               cgACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTc                          <  2:4416182/93‑1 (MQ=255)

                                                                gACTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCa                         >  1:553173/1‑93 (MQ=255)

                                                                 aCTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAg                        >  2:6153392/1‑93 (MQ=255)

                                                                 aCTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAg                        >  2:7261511/1‑93 (MQ=255)

                                                                  cTGCCTTCTATGCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                       <  1:2553629/93‑1 (MQ=255)

                                                                  cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                       <  1:2838002/93‑1 (MQ=255)

                                                                  cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                       <  1:2999566/93‑1 (MQ=255)

                                                                  cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                       >  1:8306898/1‑93 (MQ=255)

                                                                  cTGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGa                       <  2:518652/93‑1 (MQ=255)

                                                                   tGCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGAc                      >  2:3571345/1‑93 (MQ=255)

                                                                    gCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACg                     >  2:10043729/1‑93 (MQ=255)

                                                                    gCCTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACg                     <  2:9551524/93‑1 (MQ=255)

                                                                     ccTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGc                    <  2:6983655/93‑1 (MQ=255)

                                                                      cTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCa                   >  2:5473139/1‑93 (MQ=255)

                                                                      cTTCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCa                   >  2:8222489/1‑93 (MQ=255)

                                                                       ttCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCCAACCGACGTTCAAACATTTTACGTACGACCTTCAGACGaaa                  >  1:2689850/1‑93 (MQ=255)

                                                                       ttCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCaa                  >  1:9766894/1‑93 (MQ=255)

                                                                       ttCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCaa                  >  2:4720241/1‑93 (MQ=255)

                                                                       ttCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGGTCAAACATTTTACGTACGACCTTCAGACGaaa                  >  2:2717844/1‑93 (MQ=255)

                                                                        tCTATACCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCaaa                 >  1:5633958/1‑93 (MQ=255)

                                                                          tataCCTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATc               <  1:1237776/93‑1 (MQ=255)

                                                                              ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa           >  2:6817759/1‑93 (MQ=255)

                                                                              ccTCTGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCa           >  2:9095965/1‑93 (MQ=255)

                                                                                  tGAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCa       <  2:6589514/93‑1 (MQ=255)

                                                                                   gAGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCag      <  2:37850/93‑1 (MQ=255)

                                                                                    aGTTGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCaga     <  1:3073330/93‑1 (MQ=255)

                                                                                       tGCCCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGCa  >  2:2548074/1‑93 (MQ=255)

TAGCGACGTCCATTCCATGTCACGCCGTATTGCTTCGCCAGAAGTGCGTAGGTTGCCCGCACGCGACTGCCTTCTATACCTCTGAGTTGCTCTACGGAGCGCCGGGCAGGCGCAGGTTCTCCAAACCGAAGTTCAAACATTTTACGTACGACCTTCAGACGCAAATCTTCATCCAGAGCA  >  NC_000913/2877302‑2877481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`