BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,924,563	T→C	N78N (AAT→AAC)	ygdH →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,924,563	0	T	C	92.3%	1086.9 / 58.3	409	N78N (AAT→AAC)	ygdH	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (18/13); new base C (164/208); total (186/223)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-02

Only 100 of 452 total aligned reads displayed.

ACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTA  >  NC_000913/2924471‑2924652

 ccGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATc                                                                                          >  2:2034640/1‑93 (MQ=255)

 ccGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACGGATTAATc                                                                                          >  2:10001187/1‑93 (MQ=255)

  cGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAccc                                                                                         >  2:7016039/1‑93 (MQ=255)

   gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccc                                                                                        >  2:3273690/1‑93 (MQ=255)

   gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccc                                                                                        <  1:8390493/93‑1 (MQ=255)

   gATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTCAACGCGGCGTAAAGCTGGAACTGATTAAcccc                                                                                        >  1:5414280/1‑93 (MQ=255)

    aTAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACcccc                                                                                       >  2:8482051/1‑93 (MQ=255)

     tAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccccc                                                                                      <  1:2997838/93‑1 (MQ=255)

     tAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAcccccc                                                                                      >  2:8055879/1‑93 (MQ=255)

      aaCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCg                                                                                     <  2:9086529/93‑1 (MQ=255)

      aaCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAAACCCCCg                                                                                     >  2:3864054/1‑93 (MQ=255)

       aCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCgg                                                                                    >  1:3268474/1‑93 (MQ=255)

       aCAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCgg                                                                                    >  1:10043578/1‑93 (MQ=255)

            aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAagag                                                                               >  1:2308961/1‑93 (MQ=255)

            aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAagag                                                                               <  2:12962/93‑1 (MQ=255)

            aaaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAagag                                                                               <  2:3654884/93‑1 (MQ=255)

             aaGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAgg                                                                              >  1:3920245/1‑93 (MQ=255)

               gAATTGCTGTCTCGTTTTGCAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTACCCCCCCGGAAGAGGCt                                                                            <  2:8959463/93‑1 (MQ=255)

               gAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCt                                                                            >  1:9201143/1‑93 (MQ=255)

                aaTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCtt                                                                           >  1:8664801/1‑93 (MQ=255)

                 aTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCttt                                                                          <  1:4245191/93‑1 (MQ=255)

                 aTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCttt                                                                          >  1:1642692/1‑93 (MQ=255)

                 aTTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCttt                                                                          <  2:6403500/93‑1 (MQ=255)

                  ttGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCtttt                                                                         >  1:4516750/1‑93 (MQ=255)

                    gCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGt                                                                       <  1:7652229/93‑1 (MQ=255)

                    gCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGt                                                                       <  2:8211744/93‑1 (MQ=255)

                      tGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCg                                                                     >  1:5568839/1‑93 (MQ=255)

                      tGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCg                                                                     >  2:5463313/1‑93 (MQ=255)

                       gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAGCTGATTAACCCCCCGGAAGAGGCTTTTGTCGa                                                                    >  2:3530100/1‑93 (MQ=255)

                       gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGa                                                                    <  1:5202667/93‑1 (MQ=255)

                       gTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGa                                                                    <  1:1758437/93‑1 (MQ=255)

                        tctcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGAt                                                                   >  1:8271237/1‑93 (MQ=255)

                         ctcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATg                                                                  <  2:5926627/93‑1 (MQ=255)

                         ctcGTTTTGAAAATTTCGAGATTAACGTCTTGCGACGTGAACGCGGCGTAAAGCTGGAACTGAGTAACCCCCCGGAAGAGGCTTTTGTCGATg                                                                  <  1:8195123/93‑1 (MQ=255)

                          tcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATgg                                                                 <  1:760021/93‑1 (MQ=255)

                          tcGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATgg                                                                 >  2:4702806/1‑93 (MQ=255)

                           cGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATggg                                                                >  2:3979533/1‑93 (MQ=255)

                            gTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGc                                                               >  2:7648714/1‑93 (MQ=255)

                            gTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGc                                                               <  2:9847480/93‑1 (MQ=255)

                             ttttGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCg                                                              >  2:1163116/1‑93 (MQ=255)

                                 gAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAatt                                                          <  2:7525059/93‑1 (MQ=255)

                                  aaaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAatta                                                         <  2:9797656/93‑1 (MQ=255)

                                  aaaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAatta                                                         <  1:956072/93‑1 (MQ=255)

                                   aaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAattat                                                        >  2:2667447/1‑93 (MQ=255)

                                   aaaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAattat                                                        >  2:2111743/1‑93 (MQ=255)

                                    aaTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAattatt                                                       >  2:2817953/1‑93 (MQ=255)

                                     aTTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTc                                                      <  2:1726952/93‑1 (MQ=255)

                                      tttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                     <  1:7962482/93‑1 (MQ=255)

                                      tttCGATATTAACGTCGTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcg                                                     <  1:7164957/93‑1 (MQ=255)

                                       ttCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTcgc                                                    <  1:7795352/93‑1 (MQ=255)

                                           atatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCtt                                                >  1:6144630/1‑93 (MQ=255)

                                           atatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCtt                                                <  1:6828771/93‑1 (MQ=255)

                                           atatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCtt                                                <  1:9819776/93‑1 (MQ=255)

                                            tatTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCTGAAGAGGCTTTTGTCGATGGGCGAATTATTGGCGGttt                                               >  2:2473273/1‑93 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             >  2:1600677/1‑93 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             <  2:927046/93‑1 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             >  2:5771454/1‑93 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             <  1:4091076/93‑1 (MQ=255)

                                              ttAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGCAACTGATTAGCCCCCCGGAAGAGTCTTTTGTCGATGGGCGAATTATTCGCCCTTTGc                                             <  2:6474693/93‑1 (MQ=255)

                                              tcaaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGc                                             <  2:9593721/91‑1 (MQ=255)

                                               tAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCa                                            <  1:3728431/93‑1 (MQ=255)

                                               tAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCa                                            <  2:88543/93‑1 (MQ=255)

                                                aaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAg                                           >  2:7201622/1‑93 (MQ=255)

                                                aaCGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAg                                           >  1:1884887/1‑93 (MQ=255)

                                                     cTTGCGCCGGGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCaa                                      <  1:8599963/93‑1 (MQ=255)

                                                      ttGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAc                                     <  1:2750325/93‑1 (MQ=255)

                                                      ttGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAAc                                     >  1:3456970/1‑93 (MQ=255)

                                                           ccGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGtt                                <  1:1958466/93‑1 (MQ=255)

                                                           ccGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGtt                                >  2:563282/1‑93 (MQ=255)

                                                            cGTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTc                               >  1:1190859/1‑93 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGTAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              <  2:417228/93‑1 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              >  2:6642541/1‑93 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              >  2:1936585/1‑93 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              <  1:9011742/93‑1 (MQ=255)

                                                             gTGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcg                              <  1:6580626/93‑1 (MQ=255)

                                                              tGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgc                             <  1:1392636/93‑1 (MQ=255)

                                                              tGAACGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTcgc                             >  1:9385160/1‑93 (MQ=255)

                                                                aaCGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCgg                           >  2:7739101/1‑93 (MQ=255)

                                                                aaCGCGGCGTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCgg                           <  2:6831313/93‑1 (MQ=255)

                                                                   gcggcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTcc                        <  1:6051903/93‑1 (MQ=255)

                                                                   gcggcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTcc                        <  2:3417031/93‑1 (MQ=255)

                                                                     ggcgTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTg                      <  2:9396626/93‑1 (MQ=255)

                                                                     ggcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTg                      >  2:8739025/1‑93 (MQ=255)

                                                                      gcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGc                     <  2:8656202/93‑1 (MQ=255)

                                                                      gcgTAAAGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGc                     >  2:4954264/1‑93 (MQ=255)

                                                                            aGCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACa               >  1:3560501/1‑93 (MQ=255)

                                                                             gCTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAt              >  2:4802514/1‑93 (MQ=255)

                                                                              cTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAtt             >  1:5869323/1‑93 (MQ=255)

                                                                              cTGGAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACAtt             <  1:4310529/93‑1 (MQ=255)

                                                                               tGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtc            >  1:7437672/1‑93 (MQ=255)

                                                                                ggAACTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtct           >  1:2627055/1‑93 (MQ=255)

                                                                                 gAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATtctc          <  1:4874977/93‑1 (MQ=255)

                                                                                  aaCTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTctct         <  1:2159263/93‑1 (MQ=255)

                                                                                    cTGATTACCCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  2:4430021/93‑1 (MQ=255)

                                                                                    cTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  2:7827685/93‑1 (MQ=255)

                                                                                    cTGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  2:5164894/93‑1 (MQ=255)

                                                                                    atGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTc       <  1:1440820/92‑1 (MQ=255)

                                                                                     tGATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTct      >  1:6350786/1‑92 (MQ=255)

                                                                                      gATTAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGt     >  2:2357697/1‑93 (MQ=255)

                                                                                        ttAACCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGttt   <  2:614464/93‑1 (MQ=255)

ACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTGATTAATCCCCCGGAAGAGGCTTTTGTCGATGGGCGAATTATTCGCGCTTTGCAGGCCAACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTA  >  NC_000913/2924471‑2924652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`