BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,933,024	T→C	R256R (CGT→CGC)	fucP →	fucose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,933,024	0	T	C	99.8%	1399.7 / ‑5.3	429	R256R (CGT→CGC)	fucP	fucose permease
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (166/262); total (167/262)
Fisher's exact test for biased strand distribution p-value = 3.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 452 total aligned reads displayed.

ATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTA  >  NC_000913/2932935‑2933114

aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                         >  1:5681748/1‑93 (MQ=255)

aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                         <  2:2075291/93‑1 (MQ=255)

aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                         <  2:8932915/93‑1 (MQ=255)

aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                         <  2:10002661/93‑1 (MQ=255)

aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                         <  2:8723712/93‑1 (MQ=255)

aTGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCAtt                                                                                         <  1:6310006/93‑1 (MQ=255)

 tGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTc                                                                                        >  1:580368/1‑93 (MQ=255)

   cTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGc                                                                                      >  2:8244221/1‑93 (MQ=255)

     gACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCa                                                                                    >  1:4582246/1‑93 (MQ=255)

     gACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCa                                                                                    <  2:6291954/93‑1 (MQ=255)

      aCGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCAc                                                                                   <  2:2621072/93‑1 (MQ=255)

       cGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACt                                                                                  <  2:5054335/93‑1 (MQ=255)

       cGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACt                                                                                  >  1:2009928/1‑93 (MQ=255)

       cGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACt                                                                                  <  2:2723131/93‑1 (MQ=255)

         aaaTTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTgg                                                                                <  1:8985660/93‑1 (MQ=255)

          aaTTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGgc                                                                               >  1:1953059/1‑93 (MQ=255)

            ttCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGgcgc                                                                             <  1:3287811/93‑1 (MQ=255)

             tCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCt                                                                            >  2:6654031/1‑93 (MQ=255)

              cccGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTg                                                                           <  1:5046539/93‑1 (MQ=255)

                cGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTggg                                                                         <  2:9736212/93‑1 (MQ=255)

                cGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTggg                                                                         >  1:1335093/1‑93 (MQ=255)

                  gCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCg                                                                       <  2:4885573/93‑1 (MQ=255)

                   cATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCgg                                                                      <  1:4817915/93‑1 (MQ=255)

                    aTTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGt                                                                     <  2:8060588/93‑1 (MQ=255)

                     gtatagagTGATACTCACAGTGACCCAAAATAAGAATCGTTCTCCGCATCTCTTTCTCGCCTGGCGCGCAGTCGCCACTGGCGCTGGGCGGTa                                                                    <  2:4420456/89‑1 (MQ=255)

                      tGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTAt                                                                   <  1:3365434/93‑1 (MQ=255)

                       gCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTAtt                                                                  >  1:8367901/1‑93 (MQ=255)

                       gCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTAtt                                                                  <  2:593209/93‑1 (MQ=255)

                        cAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTa                                                                 >  1:6244979/1‑93 (MQ=255)

                        cAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTa                                                                 <  2:7811953/93‑1 (MQ=255)

                        cAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTa                                                                 >  2:3756894/1‑93 (MQ=255)

                        cAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTGTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTa                                                                 <  1:246076/93‑1 (MQ=255)

                         agagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAg                                                                >  1:9885827/1‑93 (MQ=255)

                          gagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAgc                                                               >  1:3488242/1‑93 (MQ=255)

                          gagTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAgc                                                               >  1:9466472/1‑93 (MQ=255)

                             tGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCa                                                            >  2:8457680/1‑93 (MQ=255)

                             tGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCa                                                            >  2:9662793/1‑93 (MQ=255)

                             tGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCa                                                            >  1:2820552/1‑93 (MQ=255)

                              gATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCaa                                                           <  1:7762103/93‑1 (MQ=255)

                                tAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAtt                                                         <  2:645548/93‑1 (MQ=255)

                                tAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAAtt                                                         >  2:359930/1‑93 (MQ=255)

                                 aaTCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTATCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGCATTAGCGCAAttt                                                        >  2:565962/1‑92 (MQ=255)

                                  aTCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCt                                                       <  1:6022724/93‑1 (MQ=255)

                                   tCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTg                                                      >  1:409255/1‑93 (MQ=255)

                                   tCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTg                                                      >  2:1991168/1‑93 (MQ=255)

                                   acacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTGCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTg                                                      <  1:8543467/92‑1 (MQ=255)

                                    cacaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGc                                                     <  1:3959298/93‑1 (MQ=255)

                                     acaGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCt                                                    <  2:2244576/93‑1 (MQ=255)

                                      caGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTa                                                   <  2:731533/93‑1 (MQ=255)

                                        gTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATg                                                 <  2:2876942/93‑1 (MQ=255)

                                         tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                                <  2:1232358/93‑1 (MQ=255)

                                         tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                                <  2:7006267/93‑1 (MQ=255)

                                         tGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGt                                                >  1:5434592/1‑93 (MQ=255)

                                          taCGCCAAACAAAAAACGTTCTCCGCATCGCTTTCTCTCCTGGCGCGCATTCGCAACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTc                                               <  1:7457730/92‑1 (MQ=255)

                                          gACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTc                                               <  2:5789653/93‑1 (MQ=255)

                                             gCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGgc                                            <  1:433753/93‑1 (MQ=255)

                                             gCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGgc                                            <  1:1790590/93‑1 (MQ=255)

                                                aaaCAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGca                                         <  1:1139571/93‑1 (MQ=255)

                                                     aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                    <  1:4955630/93‑1 (MQ=255)

                                                     aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                    >  2:4620448/1‑93 (MQ=255)

                                                     aGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAAc                                    <  1:7615339/93‑1 (MQ=255)

                                                      ggATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACACACg                                   >  2:1833746/1‑93 (MQ=255)

                                                      ggATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACg                                   <  1:1659199/93‑1 (MQ=255)

                                                      ggATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACg                                   <  1:4477407/93‑1 (MQ=255)

                                                       gATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACgg                                  >  2:3008625/1‑93 (MQ=255)

                                                       gATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACgg                                  <  2:7403072/93‑1 (MQ=255)

                                                        aTCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGc                                 >  2:1149720/1‑93 (MQ=255)

                                                         tCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGcc                                <  2:324006/93‑1 (MQ=255)

                                                           cttCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctg                              <  2:8573820/92‑1 (MQ=255)

                                                            ttCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctgc                             <  2:4211338/93‑1 (MQ=255)

                                                            ttCTCCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctgc                             <  1:6696219/93‑1 (MQ=255)

                                                             tctcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctgct                            >  2:2343229/1‑93 (MQ=255)

                                                              ctcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCctgctg                           <  2:2537588/93‑1 (MQ=255)

                                                               tcCGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTgg                          >  1:10018309/1‑93 (MQ=255)

                                                                ccGCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGa                         <  1:3600784/93‑1 (MQ=255)

                                                                  gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                       >  1:3055777/1‑93 (MQ=255)

                                                                  gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                       <  2:5204192/93‑1 (MQ=255)

                                                                  gCATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                       <  2:8748424/93‑1 (MQ=255)

                                                                  ccctCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGc                       <  1:2278488/90‑1 (MQ=255)

                                                                   cATCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCt                      <  1:7289588/93‑1 (MQ=255)

                                                                     tCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAt                    <  2:1544457/93‑1 (MQ=255)

                                                                     tCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAt                    <  2:1617084/93‑1 (MQ=255)

                                                                     tCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAt                    >  1:6515868/1‑93 (MQ=255)

                                                                     tCGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAt                    <  2:7163224/93‑1 (MQ=255)

                                                                      cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                   <  2:3675206/93‑1 (MQ=255)

                                                                      cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                   <  1:9147929/93‑1 (MQ=255)

                                                                      cGCTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTAtt                   <  2:531099/93‑1 (MQ=255)

                                                                        cTTTCTCGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTg                 >  2:9154824/1‑93 (MQ=255)

                                                                           tctcGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGAtt              >  1:9382915/1‑93 (MQ=255)

                                                                             tcGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCg            <  2:4348761/93‑1 (MQ=255)

                                                                              cGCCTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGc           <  1:147759/93‑1 (MQ=255)

                                                                                cccggCGCGCATTCGCCACGGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa         <  2:40074/90‑1 (MQ=255)

                                                                                ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa         <  2:854233/93‑1 (MQ=255)

                                                                                ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa         >  2:1073278/1‑93 (MQ=255)

                                                                                ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa         >  1:7031731/1‑93 (MQ=255)

                                                                                ccTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTa         >  2:9565881/1‑93 (MQ=255)

                                                                                 cTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAc        <  1:7873048/93‑1 (MQ=255)

                                                                                 cTGGCGCGCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTAc        <  2:3446610/93‑1 (MQ=255)

                                                                                     cgcgCATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTg    <  2:679409/93‑1 (MQ=255)

                                                                                      gcgcATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGt   >  1:5750345/1‑93 (MQ=255)

ATGCTGACGAAATTCCCGGCATTGCAGAGTGATAATCACAGTGACGCCAAACAAGGATCGTTCTCCGCATCGCTTTCTCGCCTGGCGCGTATTCGCCACTGGCGCTGGGCGGTATTAGCGCAATTCTGCTATGTCGGCGCACAAACGGCCTGCTGGAGCTATTTGATTCGCTACGCTGTA  >  NC_000913/2932935‑2933114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`