BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,854	T→C	L271P (CTG→CCG)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,854	0	T	C	99.7%	904.5 / NA	286	L271P (CTG→CCG)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (135/150); total (136/150)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 301 total aligned reads displayed.

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

 ggtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGAt                                                                                        <  1:2434661/93‑1 (MQ=255)

 ggtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGAt                                                                                        >  1:8273587/1‑93 (MQ=255)

 ggtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGAt                                                                                        >  2:3523355/1‑93 (MQ=255)

  gtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATc                                                                                       <  1:4156134/93‑1 (MQ=255)

  gtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATc                                                                                       >  1:2118820/1‑93 (MQ=255)

  gtggtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATc                                                                                       <  1:8557327/93‑1 (MQ=255)

     gtTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCAGc                                                                                    >  1:4529720/1‑93 (MQ=255)

      ttAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGcc                                                                                   >  2:9105741/1‑93 (MQ=255)

      ttAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGcc                                                                                   >  1:3654257/1‑93 (MQ=255)

       tAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACAGTCAGACCCAACCGccc                                                                                  >  1:8901353/1‑93 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGccccc                                                                                >  1:7892091/1‑93 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGccccc                                                                                <  2:4167027/93‑1 (MQ=255)

         aCCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGccccc                                                                                <  1:8158744/93‑1 (MQ=255)

           ccGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTg                                                                              >  1:9052856/1‑93 (MQ=255)

              cTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAc                                                                           >  1:1350424/1‑93 (MQ=255)

              cTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAc                                                                           <  2:9023603/93‑1 (MQ=255)

              cTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAc                                                                           <  2:96343/93‑1 (MQ=255)

               tCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAcc                                                                          >  1:2741044/1‑93 (MQ=255)

               tCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCAcc                                                                          <  2:2328786/93‑1 (MQ=255)

                 aCCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGc                                                                        >  1:6708968/1‑93 (MQ=255)

                  ccGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGcc                                                                       <  2:5551862/93‑1 (MQ=255)

                   cGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGccc                                                                      <  2:7882272/93‑1 (MQ=255)

                    gCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCg                                                                     <  1:9151676/93‑1 (MQ=255)

                    gCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCg                                                                     <  2:1854860/93‑1 (MQ=255)

                     cACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGt                                                                    >  2:8012307/1‑93 (MQ=255)

                      aCTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtc                                                                   >  2:2108917/1‑93 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcc                                                                  >  2:7548183/1‑92 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtca                                                                  >  1:2647448/1‑93 (MQ=255)

                       cTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtca                                                                  <  1:4893143/93‑1 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  2:3715523/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 >  2:5092340/1‑93 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcat                                                                 <  2:1172270/93‑1 (MQ=255)

                        tAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCACCCCCTGCCCCGCCCGtcat                                                                 <  2:4248616/93‑1 (MQ=255)

                         aGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatc                                                                <  1:5029165/93‑1 (MQ=255)

                          gATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGtcatca                                                               <  2:379982/93‑1 (MQ=255)

                          gATTATGCGGCAAGCGGTTGAGTGGGGCGGGGTTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCCCCGCCCGtcatca                                                               <  2:8174569/93‑1 (MQ=255)

                           aTTAGGCTGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAg                                                              <  2:8509552/93‑1 (MQ=255)

                             tATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcg                                                            >  1:839973/1‑93 (MQ=255)

                             tATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcg                                                            <  1:9443697/93‑1 (MQ=255)

                             tATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTACACCGCCCGTCATCAgcg                                                            <  2:836880/93‑1 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           >  1:1013926/1‑93 (MQ=255)

                              aTGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAgcgc                                                           <  2:4935877/93‑1 (MQ=255)

                                gCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTg                                                         <  1:6828094/93‑1 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    >  2:1710436/1‑93 (MQ=255)

                                     aaGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCACCTGCACCGCCCGTCATCAGCGCTGGTTCa                                                    >  1:5734077/1‑93 (MQ=255)

                                      aGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAg                                                   <  2:9412739/93‑1 (MQ=255)

                                       gCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAgg                                                  <  1:7569338/93‑1 (MQ=255)

                                       gCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAgg                                                  <  1:86815/93‑1 (MQ=255)

                                        gggTTTAGAGGCGCGGCGGTTCAGTATATCGGTTCCGTTCGCCGTCAGCCCGCTCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGc                                                 <  1:7722780/92‑1 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               >  2:4439263/1‑93 (MQ=255)

                                          gTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGt                                               >  1:2948293/1‑93 (MQ=255)

                                           tttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGtt                                              <  1:3230405/93‑1 (MQ=255)

                                            ttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGTCgcgg                                             <  2:2991608/93‑4 (MQ=255)

                                            ttAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGttt                                             <  2:3153490/93‑1 (MQ=255)

                                             tAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTa                                            <  1:9333123/93‑1 (MQ=255)

                                             tAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTa                                            <  1:1645037/93‑1 (MQ=255)

                                             tAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTa                                            >  1:7249495/1‑93 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAg                                           <  2:6279526/93‑1 (MQ=255)

                                              aGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAg                                           >  2:3276905/1‑93 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          >  2:399473/1‑93 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          >  2:6521674/1‑93 (MQ=255)

                                               gtggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGt                                          <  2:4553110/93‑1 (MQ=255)

                                                tggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTg                                         <  1:4588702/93‑1 (MQ=255)

                                                tggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTg                                         <  2:6987329/93‑1 (MQ=255)

                                                tggtgCGGCGGTTCAGAATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTg                                         >  2:9559966/1‑93 (MQ=255)

                                                 ggtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTgg                                        <  2:3399853/93‑1 (MQ=255)

                                                  gtgCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTggg                                       <  2:5118520/93‑1 (MQ=255)

                                                    gcggcgGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGATCAGGCGTTTAGTGGGCa                                     <  2:2874893/93‑1 (MQ=255)

                                                       gcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAttt                                  >  1:9852173/1‑93 (MQ=255)

                                                       gcggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCAttt                                  >  1:3781216/1‑93 (MQ=255)

                                                         ggTTCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTAc                                >  2:1075584/1‑93 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             >  2:7424160/1‑93 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             >  1:8078832/1‑93 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             <  1:8822350/93‑1 (MQ=255)

                                                            tCAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAg                             >  1:2225236/1‑93 (MQ=255)

                                                            tCAGTATTGCGGATCCGTTTACTGTCACGCCGATCCGCGCCCTTTATCGCCCGTCATTACCGCCGGGTCAGGCGTTTAGTGGGCCTTTACAAg                             >  2:7379897/1‑93 (MQ=255)

                                                             cAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGc                            <  1:2539348/93‑1 (MQ=255)

                                                             cAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGc                            >  1:3143620/1‑93 (MQ=255)

                                                             cAGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGc                            <  1:710414/93‑1 (MQ=255)

                                                              aGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCg                           <  1:9898183/93‑1 (MQ=255)

                                                              aGTATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCg                           <  1:514724/93‑1 (MQ=255)

                                                                tATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         >  1:1979962/1‑93 (MQ=255)

                                                                tATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         <  2:4658167/93‑1 (MQ=255)

                                                                tATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         >  2:531210/1‑93 (MQ=255)

                                                                tATTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         <  2:2383446/93‑1 (MQ=255)

                                                                tATTGCCGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCggg                         >  2:9883003/1‑93 (MQ=255)

                                                                 agtGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgggg                        <  1:4479617/91‑1 (MQ=255)

                                                                 aTTGCGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCgggg                        <  2:8199719/93‑1 (MQ=255)

                                                                     cGGTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTAc                    <  1:692011/93‑1 (MQ=255)

                                                                       gTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCg                  >  1:2001364/1‑93 (MQ=255)

                                                                       gTTCCGTTCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCg                  <  2:4879302/93‑1 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTg            >  1:5559177/1‑93 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTg            >  2:6785448/1‑93 (MQ=255)

                                                                             ttCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTg            <  1:468931/93‑1 (MQ=255)

                                                                              tCACCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTgg           >  1:6394481/1‑93 (MQ=255)

                                                                                cccGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTc         <  1:7849115/92‑1 (MQ=255)

                                                                                aCCGTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCTCTGGTTCAGGCGTTTAGTGGGCATTTCCAAGCGGGGTTGCCTAAACTGGTc         <  2:6110933/93‑1 (MQ=255)

                                                                                   gTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACt      >  1:3315149/1‑93 (MQ=255)

                                                                                   gTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACt      >  1:978126/1‑93 (MQ=255)

                                                                                   gTCAGCCCGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACt      >  2:277501/1‑93 (MQ=255)

CGGTGGTTAACCCGCTCACCGCACTAGATTATGCGGCAAGCGGTTTAGTGGTGCGGCGGTTCAGTATTGCGGTTCCGTTCACCGTCAGCCTGATCCGCCCCCTGCACCGCCCGTCATCAGCGCTGGTTCAGGCGTTTAGTGGGCATTTACAAGCGGGGTTACCGAAACTGGTCACTTCTC  >  NC_000913/2977764‑2977943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`