BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,938	T→C	T276A (ACG→GCG)	xerD ←	XerD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,938	0	T	C	99.8%	1302.6 / ‑5.1	408	T276A (ACG→GCG)	xerD	XerD
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (181/226); total (181/227)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01

Only 100 of 446 total aligned reads displayed.

TTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCA  >  NC_000913/3036848‑3037027

ttCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcgg                                                                                         <  2:4940476/93‑1 (MQ=255)

 tCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcggg                                                                                        >  1:4655811/1‑92 (MQ=255)

  cgcGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcggtg                                                                                       <  2:53696/91‑1 (MQ=255)

  cccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcggtg                                                                                       >  2:9175972/1‑93 (MQ=255)

  cccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcggtg                                                                                       >  2:3386965/1‑93 (MQ=255)

   ccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGcggtgg                                                                                      <  1:9206070/93‑1 (MQ=255)

   ccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGcggtgg                                                                                      <  2:8101901/93‑1 (MQ=255)

     gTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGgag                                                                                    >  2:3763600/1‑93 (MQ=255)

     gTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGGAGCGACATGCGTATAAATTTGCGCGGTGgag                                                                                    >  1:53712/1‑93 (MQ=255)

      ttCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGgaga                                                                                   >  1:7701651/1‑93 (MQ=255)

        cTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGgagaga                                                                                 <  1:4143383/93‑1 (MQ=255)

         ttttCAGCCATCACGCCCGCGGGGGATGCTGTTGATGAAGTTGCCGGAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGAt                                                                                <  2:2046410/93‑1 (MQ=255)

             cAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCt                                                                            >  2:5891391/1‑93 (MQ=255)

               gACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCtgt                                                                          <  2:2799802/93‑1 (MQ=255)

                   tCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGcc                                                                      >  1:8258927/1‑93 (MQ=255)

                      cGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAg                                                                   <  2:3955284/93‑1 (MQ=255)

                       gCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGt                                                                  <  1:2664808/93‑1 (MQ=255)

                       gCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGt                                                                  >  2:8557097/1‑93 (MQ=255)

                        cccGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTa                                                                 <  2:9319429/93‑1 (MQ=255)

                        cccGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTa                                                                 <  2:6556571/93‑1 (MQ=255)

                         ccGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAg                                                                >  1:5610513/1‑93 (MQ=255)

                           gcgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCa                                                              <  2:2463439/93‑1 (MQ=255)

                           gcgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCa                                                              >  1:156293/1‑93 (MQ=255)

                            cgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACGTGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCAt                                                             <  1:6050300/93‑1 (MQ=255)

                            cgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCAt                                                             >  1:9638250/1‑93 (MQ=255)

                             gggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATc                                                            >  2:4402436/1‑93 (MQ=255)

                              ggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCt                                                           <  2:5808314/93‑1 (MQ=255)

                              ggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCt                                                           <  1:1975876/93‑1 (MQ=255)

                               gTGATGCTGTTGATGAAGGTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTg                                                          <  1:3453904/93‑1 (MQ=255)

                                tGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGc                                                         >  2:8706211/1‑93 (MQ=255)

                                tGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGc                                                         <  2:7135571/93‑1 (MQ=255)

                                 gATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGca                                                        >  2:62323/1‑93 (MQ=255)

                                    gCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacca                                                     <  1:5098190/93‑1 (MQ=255)

                                    gCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacca                                                     <  2:8826683/93‑1 (MQ=255)

                                    gCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacca                                                     <  1:975707/93‑1 (MQ=255)

                                    gCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacca                                                     <  2:4711847/93‑1 (MQ=255)

                                    gCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacca                                                     <  1:8479715/93‑1 (MQ=255)

                                     cTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcaccac                                                    <  2:5078690/93‑1 (MQ=255)

                                     cTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcaccac                                                    <  1:2229801/93‑1 (MQ=255)

                                       gTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCAcgc                                                  >  1:25704/1‑93 (MQ=255)

                                           atgaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTaa                                              <  2:741004/93‑1 (MQ=255)

                                            tgaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTaaa                                             >  2:1804696/1‑93 (MQ=255)

                                            tgaAGTTGCCGCAGACGCTCGGGAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTaaa                                             <  2:1626329/93‑1 (MQ=255)

                                             gaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAAt                                            <  1:4468768/93‑1 (MQ=255)

                                              aaGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATc                                           <  1:2324969/93‑1 (MQ=255)

                                                  tGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCa                                       <  1:2977490/93‑1 (MQ=255)

                                                  tGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCa                                       <  2:7509620/93‑1 (MQ=255)

                                                    ccGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCAcc                                     <  1:6798156/93‑1 (MQ=255)

                                                      gCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCAt                                   <  1:1697434/93‑1 (MQ=255)

                                                       cAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATg                                  >  1:73561/1‑93 (MQ=255)

                                                        aGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGa                                 >  1:1460868/1‑93 (MQ=255)

                                                          aCGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAtt                               <  1:9900437/93‑1 (MQ=255)

                                                           cGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAttt                              <  1:8794331/93‑1 (MQ=255)

                                                            gCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTta                             >  1:6130639/1‑93 (MQ=255)

                                                            gCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTta                             >  2:6821088/1‑93 (MQ=255)

                                                              tCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaat                           <  1:9269233/93‑1 (MQ=255)

                                                              tCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaat                           <  2:2170833/93‑1 (MQ=255)

                                                              tCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaat                           >  1:7003547/1‑93 (MQ=255)

                                                               cGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata                          <  1:3820143/93‑1 (MQ=255)

                                                               cGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata                          >  1:4906213/1‑93 (MQ=255)

                                                                ggTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaataa                         <  1:3125685/93‑1 (MQ=255)

                                                                ggTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaataa                         >  1:3259504/1‑93 (MQ=255)

                                                                ggTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaataa                         <  2:3586777/93‑1 (MQ=255)

                                                                ggTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaataa                         <  1:4322317/93‑1 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGTGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                        >  1:4817841/1‑93 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                        <  2:9845571/93‑1 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                        <  1:4750575/93‑1 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                        <  1:5565359/93‑1 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                        <  2:3133192/93‑1 (MQ=255)

                                                                  tAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt                       <  2:9009570/93‑1 (MQ=255)

                                                                  tAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt                       >  2:9666882/1‑93 (MQ=255)

                                                                  tAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt                       >  1:9471163/1‑93 (MQ=255)

                                                                   aGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATg                      <  2:1015704/93‑1 (MQ=255)

                                                                   aGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATg                      >  2:7778858/1‑93 (MQ=255)

                                                                   aGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATg                      <  1:6312951/93‑1 (MQ=255)

                                                                    gCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGa                     <  2:5462058/93‑1 (MQ=255)

                                                                    gCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGa                     >  2:5235307/1‑93 (MQ=255)

                                                                    gCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGa                     >  1:1067082/1‑93 (MQ=255)

                                                                     cGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAg                    <  2:8280326/93‑1 (MQ=255)

                                                                      gACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGt                   <  2:4761661/93‑1 (MQ=255)

                                                                       aCATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTg                  >  1:6456791/1‑93 (MQ=255)

                                                                       aCATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTg                  >  1:7172904/1‑93 (MQ=255)

                                                                       aCATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTg                  <  2:1271062/93‑1 (MQ=255)

                                                                         aTGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGc                <  2:4032089/93‑1 (MQ=255)

                                                                          tGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCGCGACGCGTAAATCCGCACCATGATTTAATAAATCGGTGGCa               >  1:8663632/1‑93 (MQ=255)

                                                                          tGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCa               >  2:7693151/1‑93 (MQ=255)

                                                                           gCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCaa              >  2:1461749/1‑93 (MQ=255)

                                                                             gTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCaaaa            >  2:7009928/1‑93 (MQ=255)

                                                                               ataAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGc          >  2:6290784/1‑93 (MQ=255)

                                                                               ataAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGc          >  2:5721893/1‑93 (MQ=255)

                                                                                taAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCg         <  2:4201231/93‑1 (MQ=255)

                                                                                taAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCg         >  2:944803/1‑93 (MQ=255)

                                                                                  aaTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTg       >  1:5315499/1‑93 (MQ=255)

                                                                                   aTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa      <  1:3995449/93‑1 (MQ=255)

                                                                                   aTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa      <  1:1309148/93‑1 (MQ=255)

                                                                                   aTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa      <  1:1233621/93‑1 (MQ=255)

                                                                                    tttGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGAc     >  2:5277922/1‑93 (MQ=255)

                                                                                    tttGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGAc     >  1:9025363/1‑93 (MQ=255)

                                                                                    tttGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGAc     <  1:6303308/93‑1 (MQ=255)

                                                                                      tGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGc   >  2:3719055/1‑93 (MQ=255)

TTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACGCA  >  NC_000913/3036848‑3037027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`