BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,047,558	T→C	V4V (GTA→GTG)	gcvH ←	GcvH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,047,558	0	T	C	99.4%	1636.8 / ‑2.1	513	V4V (GTA→GTG)	gcvH	GcvH
Reads supporting (aligned to +/- strand): ref base T (2/1); new base C (295/215); total (297/216)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.29e-01

Only 100 of 529 total aligned reads displayed.

CTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGC  >  NC_000913/3047468‑3047647

cTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCAc                                                                                         >  1:5523729/1‑93 (MQ=255)

 tGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTCTTTCAGTTCTGCTGGCACg                                                                                        >  1:2892998/1‑93 (MQ=255)

 tGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACg                                                                                        >  2:1950388/1‑93 (MQ=255)

  gAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGt                                                                                       >  2:6057903/1‑93 (MQ=255)

  gAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGt                                                                                       >  2:8910543/1‑93 (MQ=255)

     cATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGc                                                                                    >  1:8302540/1‑93 (MQ=255)

      aTGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCt                                                                                   >  1:6424256/1‑93 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                  >  2:6975144/1‑93 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                  >  2:4756801/1‑93 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                  >  2:9685387/1‑93 (MQ=255)

       tGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtc                                                                                  >  1:4538390/1‑93 (MQ=255)

        gTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtca                                                                                 <  2:4529407/93‑1 (MQ=255)

         ttCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcat                                                                                <  2:8735664/93‑1 (MQ=255)

         ttCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcat                                                                                <  1:4226182/93‑1 (MQ=255)

          tCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcatc                                                                               >  1:461126/1‑93 (MQ=255)

           cGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCtcatca                                                                              <  1:5653799/93‑1 (MQ=255)

            gggaaTACCAACGGTTTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaa                                                                             <  2:8162323/90‑1 (MQ=255)

            ggTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaa                                                                             >  2:3676518/1‑93 (MQ=255)

             gTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCatcaat                                                                            <  1:4796806/93‑1 (MQ=255)

                 tACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATcaatcaat                                                                        >  1:9535962/1‑93 (MQ=255)

                  aCCAAGGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCaatcaatc                                                                       <  2:7955035/93‑1 (MQ=255)

                   ccAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAAtct                                                                      <  2:1437877/93‑1 (MQ=255)

                    cAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAAtctc                                                                     >  2:910631/1‑93 (MQ=255)

                    cAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAAtctc                                                                     >  2:3381392/1‑93 (MQ=255)

                     aaCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTcc                                                                    >  2:8534177/1‑93 (MQ=255)

                      aCGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCa                                                                   <  1:387116/93‑1 (MQ=255)

                        ggTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaaa                                                                 <  1:9643923/93‑1 (MQ=255)

                         gtgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaaaa                                                                >  2:8290250/1‑93 (MQ=255)

                         gtgtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaaaa                                                                >  1:816237/1‑93 (MQ=255)

                           gtAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCaaaaaa                                                              <  1:9279196/93‑1 (MQ=255)

                            tAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAg                                                             >  1:9991071/1‑93 (MQ=255)

                               gTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTaa                                                          >  1:8402541/1‑93 (MQ=255)

                                tGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTaaa                                                         >  2:5621749/1‑93 (MQ=255)

                                    gTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCAc                                                     >  2:2946603/1‑93 (MQ=255)

                                     tctgCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGTTCATCAATCAATCTCCAAAAAAGTAAATCAcg                                                    <  1:8700224/90‑1 (MQ=255)

                                     tCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCCTCAATCAATCTCCAAAAAAGTAAATCAcc                                                    >  1:3368632/1‑92 (MQ=255)

                                       ggCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGCAAATCAcgcg                                                  >  2:9814687/1‑93 (MQ=255)

                                         cttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCGAAAAAAGTAAATCACGCGAc                                                <  2:6588937/93‑1 (MQ=255)

                                          ttcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACg                                               >  1:1789156/1‑93 (MQ=255)

                                           tcttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACgg                                              >  1:1899371/1‑93 (MQ=255)

                                            cttTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGc                                             >  2:5092229/1‑93 (MQ=255)

                                             tttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCt                                            >  2:1223320/1‑93 (MQ=255)

                                             tttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCt                                            >  2:4192555/1‑93 (MQ=255)

                                             tttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCt                                            >  1:4872189/1‑93 (MQ=255)

                                              ttccGCAGCCATTCGTGTTCTTTGCTTTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCtt                                           <  2:2622270/90‑1 (MQ=255)

                                              ttACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCtt                                           <  1:5456868/93‑1 (MQ=255)

                                               tACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCttt                                          >  1:6537230/1‑93 (MQ=255)

                                               tACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCttt                                          >  2:3325978/1‑93 (MQ=255)

                                                 cGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGc                                        >  1:6908267/1‑93 (MQ=255)

                                                 cGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGc                                        >  1:2551022/1‑93 (MQ=255)

                                                    cgCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGt                                     <  1:2687773/92‑1 (MQ=255)

                                                    aGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGt                                     >  1:615073/1‑93 (MQ=255)

                                                     gCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGtt                                    >  2:7528658/1‑93 (MQ=255)

                                                     gCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGtt                                    >  1:4711907/1‑93 (MQ=255)

                                                      ccATTCGTGTTTTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTAATAAATCAATCTCAAAAAAAGTAAACCACGCGACGGCTTTGCCGTTa                                   <  2:7232344/93‑1 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTa                                   <  1:7447113/93‑1 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTa                                   >  1:7608351/1‑93 (MQ=255)

                                                      ccATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTa                                   >  1:5985592/1‑93 (MQ=255)

                                                       cATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTAc                                  <  2:5212528/93‑1 (MQ=255)

                                                       cATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATAACGCGACGGCTTTGCCGTTAc                                  >  1:2344215/1‑93 (MQ=255)

                                                         ttCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGc                                >  1:7279826/1‑93 (MQ=255)

                                                         ttCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCGCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGc                                >  2:2798171/1‑93 (MQ=255)

                                                          tCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGca                               >  2:1065060/1‑93 (MQ=255)

                                                           cGTGTTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGcac                              <  2:8341637/93‑1 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGcaca                             <  2:5783180/93‑1 (MQ=255)

                                                            gtgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGcaca                             <  2:4289223/93‑1 (MQ=255)

                                                             tgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaa                            >  2:3611279/1‑93 (MQ=255)

                                                             tgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaa                            >  1:1531882/1‑93 (MQ=255)

                                                             tgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaa                            >  2:7652218/1‑93 (MQ=255)

                                                             tgtTCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaa                            <  1:2096569/93‑1 (MQ=255)

                                                                tCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAGAAAAGGAAATCACGCGACGGCTGTGCCGTCACGCACaaaaa                         >  1:5364118/1‑93 (MQ=255)

                                                                tCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaaaa                         <  2:5929689/93‑1 (MQ=255)

                                                                tCTTTGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACaaaaa                         >  1:663072/1‑93 (MQ=255)

                                                                  tttGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACa                       >  1:4416720/1‑93 (MQ=255)

                                                                   ttGCTGTATTTCAGGTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAg                      <  1:2059376/93‑1 (MQ=255)

                                                                    tGCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAgg                     <  2:7565552/93‑1 (MQ=255)

                                                                     gCTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGt                    <  1:9158536/93‑1 (MQ=255)

                                                                      cTGTATTTCAGTTCTGCTGGCACGTTGCTCATTAATCAATCTCCAAAAAAGTAAATCACTCGACGGCTTCGCCTTGACGTACAAAAACAGGtt                   >  1:8977232/1‑93 (MQ=255)

                                                                      cTGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGtt                   <  2:6866817/93‑1 (MQ=255)

                                                                       tGTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGttt                  >  1:5165432/1‑93 (MQ=255)

                                                                        gTATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGtttt                 <  2:7728620/93‑1 (MQ=255)

                                                                         tATTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTg                >  2:3145856/1‑93 (MQ=255)

                                                                          aTTTCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGt               >  1:9889908/1‑93 (MQ=255)

                                                                           tttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTc              >  1:8018532/1‑93 (MQ=255)

                                                                            ttCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCa             <  2:2214750/93‑1 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            >  2:4989756/1‑93 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            <  2:305050/93‑1 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            >  1:10025956/1‑93 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            >  1:7521767/1‑93 (MQ=255)

                                                                             tCAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCAc            >  1:7100186/1‑93 (MQ=255)

                                                                              cAGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACt           <  2:9590516/93‑1 (MQ=255)

                                                                               aGTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACtt          >  2:6454994/1‑93 (MQ=255)

                                                                                gTTCTGCTGGCACGTTGGTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGGTTTGCCGTTACGCACAAAAACAGGGTTTGTTACttt         >  1:6795503/1‑93 (MQ=255)

                                                                                gTTCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACttt         >  2:9277625/1‑93 (MQ=255)

                                                                                 ttCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTa        >  1:582383/1‑93 (MQ=255)

                                                                                 ttCTGCTGGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTa        <  1:9755488/93‑1 (MQ=255)

                                                                                   ctgctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAAc      >  1:1210838/1‑93 (MQ=255)

                                                                                      ctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCgg   <  1:860767/93‑1 (MQ=255)

                                                                                      ctgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCgg   <  2:615401/93‑1 (MQ=255)

                                                                                       tgGCACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGc  <  2:7894587/93‑1 (MQ=255)

CTGAGCATGTTCGGTAATACCAACGGTGTAAGTGCCGTCGGCTTCTTTACGCAGCCATTCGTGTTCTTTGCTGTATTTCAGTTCTGCTGGTACGTTGCTCATCAATCAATCTCCAAAAAAGTAAATCACGCGACGGCTTTGCCGTTACGCACAAAAACAGGTTTTGTCACTTTAACCGGC  >  NC_000913/3047468‑3047647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`