BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	339,531	T→C	V48A (GTG→GCG)	yahI →	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	339,531	0	T	C	99.6%	817.3 / NA	251	V48A (GTG→GCG)	yahI	putative kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (126/124); minor base A (1/0); total (127/124)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

ACGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGC  >  NC_000913/339441‑339620

aCGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTgcgc                                                                                         >  1:2295630/1‑93 (MQ=255)

 cGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCt                                                                                        <  1:6242125/93‑1 (MQ=255)

 cGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCt                                                                                        <  1:2934849/93‑1 (MQ=255)

  gccagccaGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTg                                                                                       >  2:4562609/1‑93 (MQ=255)

   ccagccagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGa                                                                                      >  1:7764810/1‑93 (MQ=255)

   ccagccagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGa                                                                                      <  2:5575575/93‑1 (MQ=255)

     agccagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGAcc                                                                                    <  1:9165320/93‑1 (MQ=255)

     agccagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGAcc                                                                                    >  2:9660861/1‑93 (MQ=255)

      gccagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGAccc                                                                                   >  2:4078836/1‑93 (MQ=255)

        cagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCAc                                                                                 <  1:1161889/93‑1 (MQ=255)

        cagTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCAc                                                                                 <  2:4687770/93‑1 (MQ=255)

          gTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGCTTACGACATTGCGCGGACCCCCgc                                                                               >  1:551959/1‑92 (MQ=255)

          gTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACgg                                                                               >  2:9228389/1‑93 (MQ=255)

          gTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACgg                                                                               >  1:1505167/1‑93 (MQ=255)

          gTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATCACGACATTGCGCTGACCCACgg                                                                               >  1:7005181/1‑93 (MQ=255)

            cGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCa                                                                             >  2:7002118/1‑93 (MQ=255)

             gATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCaa                                                                            <  2:6546125/93‑1 (MQ=255)

              aTTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAAc                                                                           >  1:8522120/1‑93 (MQ=255)

              aTTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAAc                                                                           >  1:5826336/1‑93 (MQ=255)

               ttGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACg                                                                          >  2:4397724/1‑93 (MQ=255)

               ttGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACg                                                                          >  2:4792948/1‑93 (MQ=255)

               ttGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACg                                                                          <  1:6335406/93‑1 (MQ=255)

                tGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACgg                                                                         >  2:6663526/1‑93 (MQ=255)

                tGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACgg                                                                         >  1:90205/1‑93 (MQ=255)

                 gAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACggc                                                                        >  1:7313881/1‑92 (MQ=255)

                  aGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGc                                                                       <  1:8151132/93‑1 (MQ=255)

                  aGCATAAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGc                                                                       <  2:3775275/93‑1 (MQ=255)

                     aTCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGc                                                                    <  1:6379272/93‑1 (MQ=255)

                      tCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCa                                                                   <  1:9963455/93‑1 (MQ=255)

                      tCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCa                                                                   >  1:6189740/1‑93 (MQ=255)

                      tCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCa                                                                   <  1:4035935/93‑1 (MQ=255)

                        aggcggaggcggGCAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAgg                                                                 <  1:2872770/93‑1 (MQ=255)

                          gcggaggcgtTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTc                                                               <  2:5344053/93‑1 (MQ=255)

                          gcggaggcggTGAAAGCCGTCGCTGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTc                                                               <  2:7236041/93‑1 (MQ=255)

                          gcggaggcggTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTc                                                               >  2:4359531/1‑93 (MQ=255)

                           gggaggcggTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCg                                                              <  2:2926984/92‑1 (MQ=255)

                              aggcggTGAAAGCCGCCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGc                                                           <  2:340851/93‑1 (MQ=255)

                                gcggTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTg                                                         >  1:3950731/1‑93 (MQ=255)

                                  ggTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGa                                                       <  1:1873913/93‑1 (MQ=255)

                                  ggTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGATTCCGATTACGACATTGCGATGACCCACGGCAACGGGCAGCAGGGCGGGCTGGa                                                       >  2:2233440/1‑93 (MQ=255)

                                   gTGAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGAt                                                      >  2:7874381/1‑93 (MQ=255)

                                    ggAAAGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGAtt                                                     <  1:7112951/92‑1 (MQ=255)

                                     gAAAGCCGTCGCCGATACGGCGATGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGAttt                                                    >  2:8358750/1‑93 (MQ=39)

                                      aaaGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTa                                                   <  1:4763760/93‑1 (MQ=255)

                                      aaaGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTa                                                   <  1:2500746/93‑1 (MQ=255)

                                       aaGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTAc                                                  >  1:2370474/1‑93 (MQ=255)

                                        aGCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACg                                                 >  1:5192316/1‑93 (MQ=255)

                                         gCCGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGc                                                >  1:5541680/1‑93 (MQ=255)

                                           cGTCGCCGATACGGCGCTGGAAATGCTGGCTTCCCATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCg                                              <  1:1272966/93‑1 (MQ=255)

                                            gTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGt                                             <  2:2296249/93‑1 (MQ=255)

                                            gTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGt                                             >  2:1986675/1‑93 (MQ=255)

                                            gTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGt                                             >  1:8865332/1‑93 (MQ=255)

                                            gTCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGt                                             <  1:8345204/93‑1 (MQ=255)

                                             tCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTg                                            >  2:6967642/1‑93 (MQ=255)

                                             tCGCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTg                                            <  1:1773614/93‑1 (MQ=255)

                                               gCCGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCg                                          <  2:9755366/93‑1 (MQ=255)

                                                ccGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCgg                                         <  2:1739939/93‑1 (MQ=255)

                                                ccGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCgg                                         >  2:482298/1‑93 (MQ=255)

                                                 cGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGga                                        <  2:1209402/93‑1 (MQ=255)

                                                 cGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGga                                        >  2:2831643/1‑93 (MQ=255)

                                                 cGATACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGga                                        >  2:1928239/1‑93 (MQ=255)

                                                   aTACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGgaga                                      >  2:6057312/1‑93 (MQ=255)

                                                   aTACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGgaga                                      >  1:8221638/1‑93 (MQ=255)

                                                    tACGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGAt                                     >  1:733937/1‑93 (MQ=255)

                                                     aCGGCGCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGAtt                                    >  2:6084718/1‑93 (MQ=255)

                                                          gCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCca                               <  2:9669660/93‑1 (MQ=255)

                                                          gCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCca                               >  2:7475584/1‑93 (MQ=255)

                                                          gCTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCca                               <  2:5918988/93‑1 (MQ=255)

                                                           cTGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCcac                              <  1:7054744/93‑1 (MQ=255)

                                                            tGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCcaca                             >  2:5506097/1‑93 (MQ=255)

                                                            tGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCcaca                             >  2:2822641/1‑93 (MQ=255)

                                                            tGGAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGAGTGccagca                             >  1:6510186/1‑89 (MQ=39)

                                                             ggAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACaa                            >  2:5173425/1‑93 (MQ=255)

                                                              gAAATGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACCAg                           >  1:3429153/1‑93 (MQ=255)

                                                               aaaTGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAgc                          <  1:199000/93‑1 (MQ=255)

                                                                aaTGCTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAgcg                         >  1:9543756/1‑93 (MQ=255)

                                                                    cTGGCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGaa                     <  2:2415672/93‑1 (MQ=255)

                                                                      ggCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAgg                   <  2:15223/93‑1 (MQ=255)

                                                                      ggCTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAgg                   <  1:3345535/93‑1 (MQ=255)

                                                                        cTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGc                 <  1:10008230/93‑1 (MQ=255)

                                                                        cTTCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGc                 >  2:2591055/1‑93 (MQ=255)

                                                                         ttCCGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCt                <  1:7906454/93‑1 (MQ=255)

                                                                         ctCCGATTACGACATTGCGCCGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCt                <  1:7470983/92‑1 (MQ=255)

                                                                           ccGATTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGc              >  1:9935835/1‑93 (MQ=255)

                                                                              aTTACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCt           >  2:6296532/1‑93 (MQ=255)

                                                                               ttACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCtt          <  1:3091516/93‑1 (MQ=255)

                                                                                tACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTa         <  2:7633786/93‑1 (MQ=255)

                                                                                tACGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTa         >  1:2195565/1‑93 (MQ=255)

                                                                                 aCGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTaa        <  2:100766/93‑1 (MQ=255)

                                                                                 aCGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTaa        <  2:2738910/93‑1 (MQ=255)

                                                                                 aCGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTaa        <  2:8474031/93‑1 (MQ=255)

                                                                                  cGACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAc       >  2:3541736/1‑93 (MQ=255)

                                                                                   gACATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcg      >  1:2580896/1‑93 (MQ=255)

                                                                                    aCATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgc     >  1:3849847/1‑93 (MQ=255)

                                                                                    aCATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgc     <  1:7606692/93‑1 (MQ=255)

                                                                                    aCATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgc     <  1:9284397/93‑1 (MQ=255)

                                                                                     cATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgcc    <  1:440926/93‑1 (MQ=255)

                                                                                     cATTGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgcc    >  2:7960167/1‑93 (MQ=255)

                                                                                       ttGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgccgc  >  2:8781621/1‑93 (MQ=255)

                                                                                       ttGCGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAAcgccgc  <  1:5284385/93‑1 (MQ=255)

ACGCCAGCCAGTCGATTGAGCATCAGGCGGAGGCGGTGAAAGCCGTCGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACGGGCCGCAGGTCGGGCTGGATTTACGCCGTGCGGAGATTGCCCACAAGCGCGAAGGGCTGCCCTTAACGCCGC  >  NC_000913/339441‑339620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`