BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	361,946	A→G	L153P (CTG→CCG)	lacY ←	galactoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,946	0	A	G	99.7%	1235.7 / ‑5.5	389	L153P (CTG→CCG)	lacY	galactoside permease
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (213/175); total (213/176)
Fisher's exact test for biased strand distribution p-value = 4.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

Only 100 of 416 total aligned reads displayed.

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

aaaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgc                                                                                         >  2:4749008/1‑93 (MQ=255)

 aaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgcg                                                                                        >  2:7655594/1‑93 (MQ=255)

 aaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgcc                                                                                        >  1:3595202/1‑92 (MQ=255)

  aaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCTGACAATCGAGGCACACGgcgc                                                                                       >  1:8872377/1‑93 (MQ=255)

   aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGcc                                                                                      <  2:5582854/93‑1 (MQ=255)

     ggCGAGGATGAATGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCa                                                                                    >  2:1458954/1‑93 (MQ=255)

      gCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACACACTGATTATTGATGGTGAACATGATGACGACAATCGACGCACACGGCGCCCAg                                                                                   >  2:4522432/1‑93 (MQ=255)

       cGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGc                                                                                  <  2:5810789/93‑1 (MQ=255)

       cGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGc                                                                                  >  1:328588/1‑93 (MQ=255)

         aGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCTGACAATCGAGGCACACGGCGCCCAGCCa                                                                                >  1:6492118/1‑93 (MQ=255)

         aGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCa                                                                                <  1:755352/93‑1 (MQ=255)

          ggATGAGTGTACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCaa                                                                               >  1:9892388/1‑93 (MQ=255)

          ggATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCaa                                                                               >  2:8364316/1‑93 (MQ=255)

          ggATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCaa                                                                               >  2:2368927/1‑93 (MQ=255)

             tGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAacac                                                                            <  1:1581418/93‑1 (MQ=255)

               agggCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAg                                                                          <  1:2927943/90‑1 (MQ=255)

               aGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAAcacac                                                                          >  1:8357424/1‑92 (MQ=255)

               aGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAg                                                                          >  1:9567067/1‑93 (MQ=255)

                gTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGc                                                                         <  1:6201443/93‑1 (MQ=255)

                   cacaGCCAGAGCCCAGCCAGAAAACAAACTGATTAGTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCaa                                                                      >  1:1843012/1‑93 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   >  2:3172275/1‑93 (MQ=255)

                       gCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACAt                                                                  <  1:9195771/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  2:6230955/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  2:9303417/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 >  2:9485277/1‑93 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  2:9653988/93‑1 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                <  1:5932638/93‑1 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                <  1:2296863/93‑1 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                <  1:5416356/93‑1 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                >  2:5194154/1‑93 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                >  1:43311/1‑93 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                <  2:1273327/93‑1 (MQ=255)

                         cAGAGCCCAGCCAGAAAACAAACTGATTATTGATGATGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATcc                                                                <  2:4031241/93‑1 (MQ=255)

                          agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcg                                                               >  2:6588922/1‑93 (MQ=255)

                          agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcg                                                               >  1:8205960/1‑93 (MQ=255)

                          agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcg                                                               >  2:2027860/1‑93 (MQ=255)

                             gCCAAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgc                                                            >  2:8459257/1‑93 (MQ=255)

                               ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgc                                                          <  2:6171691/93‑1 (MQ=255)

                               ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgc                                                          >  2:5018056/1‑93 (MQ=255)

                                cagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgcg                                                         <  1:457677/93‑1 (MQ=255)

                                 agccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGa                                                        >  2:2643278/1‑93 (MQ=255)

                                 aaccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGa                                                        >  2:4345661/3‑93 (MQ=255)

                                    cagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCa                                                     >  1:8567567/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  1:1047159/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  1:6834750/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  1:6971947/1‑93 (MQ=255)

                                      gAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   >  2:8596708/1‑93 (MQ=255)

                                      aaaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   <  2:2171953/92‑1 (MQ=255)

                                       aaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACAAAAt                                                  >  1:8623899/1‑93 (MQ=255)

                                         aacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTc                                                >  2:9434412/1‑93 (MQ=255)

                                            aaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaa                                             >  2:7082328/1‑93 (MQ=255)

                                            aaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaa                                             <  1:2700141/93‑1 (MQ=255)

                                              acTGTTTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCCACACAGCCAACCATCCGCGCGCGACCAAATTCGAAAt                                           <  2:8949357/93‑1 (MQ=255)

                                              acTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAt                                           >  1:1364925/1‑93 (MQ=255)

                                              acTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAt                                           <  2:2815548/93‑1 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         <  1:5068731/93‑1 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         <  2:3773718/93‑1 (MQ=255)

                                                tGATTATTGATGGGAAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         <  1:2564343/93‑1 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        <  1:3390501/93‑1 (MQ=255)

                                                  attattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACt                                       <  1:2174939/93‑1 (MQ=255)

                                                   ttattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTg                                      >  2:6798823/1‑93 (MQ=255)

                                                   ttattGATGGTGAAAATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACAAAAGTCGAAATTACTg                                      >  2:1625454/1‑93 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  2:7894443/93‑1 (MQ=255)

                                                       tGATGGTGAACATGATGCCGACAATCGAGGCACCCGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                  <  2:3000606/93‑1 (MQ=255)

                                                       tGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                  <  2:9579152/93‑1 (MQ=255)

                                                       tGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                  >  2:6419354/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               <  1:3932001/93‑1 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               >  1:5594549/1‑93 (MQ=255)

                                                           ggTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCt                              >  1:9802210/1‑93 (MQ=255)

                                                              gAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGAc                           <  1:8324156/93‑1 (MQ=255)

                                                              gAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGAc                           <  2:77119/93‑1 (MQ=255)

                                                               aaCATGATGCCGACAATCGAGGCGCACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACt                          >  1:4904452/1‑93 (MQ=255)

                                                                aCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACtt                         <  1:6273117/93‑1 (MQ=255)

                                                                  atgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtc                       <  1:7147684/93‑1 (MQ=255)

                                                                  atgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtc                       <  2:5683395/93‑1 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  1:3021222/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  2:2549545/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  1:202741/1‑93 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      <  2:6001416/93‑1 (MQ=255)

                                                                    gatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGGGCGCGCCCAAATTCGAAATTAATGCGACGGATGACTTtctc                     >  2:4866245/1‑93 (MQ=255)

                                                                     atgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCa                    <  1:7392791/93‑1 (MQ=255)

                                                                      tgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCaa                   <  1:3852653/93‑1 (MQ=255)

                                                                         ctaCAAGCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaa                <  1:3671602/91‑1 (MQ=255)

                                                                         cGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaa                <  2:1156055/93‑1 (MQ=255)

                                                                         cGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaa                <  2:8115719/93‑1 (MQ=255)

                                                                          gACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaaa               >  1:930964/1‑93 (MQ=255)

                                                                          gACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaaa               <  1:7310993/93‑1 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              <  2:6949151/93‑1 (MQ=255)

                                                                           aCAACCGAGGCACCCGCCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              <  2:7398905/93‑1 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           >  2:4115055/1‑93 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTAAATAAATGcc           >  1:4132131/1‑93 (MQ=255)

                                                                                cGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctc         >  2:7343541/1‑93 (MQ=255)

                                                                                  aGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTa       <  1:5232584/93‑1 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      >  1:5809610/1‑93 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      <  2:3379342/93‑1 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      <  1:8815162/93‑1 (MQ=255)

                                                                                     cacacgGCGCACAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActg    <  1:4697031/93‑1 (MQ=255)

                                                                                      acacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgc   >  2:8224876/1‑93 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  <  1:7663230/93‑1 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  >  1:9040349/1‑93 (MQ=255)

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`