BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,631	A→G	E90G (GAA→GGA)	ebgC →	evolved beta‑D‑galactosidase beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,631	0	A	G	100.0%	1626.3 / NA	498	E90G (GAA→GGA)	ebgC	evolved beta‑D‑galactosidase beta subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (272/226); total (272/226)

Only 100 of 504 total aligned reads displayed.

CCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATA  >  NC_000913/3223541‑3223720

ccGGGCCTCGACGCTTTTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACCTTACAGGTAGTGGGat                                                                                         <  2:8966535/93‑1 (MQ=255)

ccGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGCATATGCGCCGAAAGAGACATTACAGGTAGTGGGat                                                                                         >  1:2635077/1‑93 (MQ=255)

ccGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGat                                                                                         <  2:1171024/93‑1 (MQ=255)

 cGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGata                                                                                        <  1:7681806/93‑1 (MQ=255)

 cGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGata                                                                                        <  2:3935201/93‑1 (MQ=255)

  gggCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGatat                                                                                       <  2:3274466/93‑1 (MQ=255)

   ggCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtatt                                                                                      <  1:4511170/93‑1 (MQ=255)

   ggCATCGACGCTATTTTGAAGAGAATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGAGGGatata                                                                                      >  2:2106472/1‑92 (MQ=255)

     cATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGCAAGAGACATTACAGGTAGTGGGAtattat                                                                                    >  2:7023983/1‑93 (MQ=255)

      aTCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATc                                                                                   <  2:5638467/93‑1 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  >  2:6514233/1‑93 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  >  2:2887010/1‑93 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  >  1:794404/1‑93 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  >  2:9461473/1‑93 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  >  2:9562719/1‑93 (MQ=255)

         gACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtg                                                                                <  2:7679126/93‑1 (MQ=255)

         gACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtg                                                                                <  1:5903733/93‑1 (MQ=255)

          aCGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtga                                                                               >  2:9721772/1‑93 (MQ=255)

          aCGCTATTTTGAAGTGCATTACGACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtga                                                                               >  2:6406044/1‑93 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              >  1:6269264/1‑93 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              >  2:5991333/1‑93 (MQ=255)

            gCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatg                                                                             >  2:3836134/1‑93 (MQ=255)

            gCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatg                                                                             >  2:9857515/1‑93 (MQ=255)

             cTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatga                                                                            >  2:2447744/1‑93 (MQ=255)

              tATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGaa                                                                           >  1:6900456/1‑93 (MQ=255)

                      aGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCg                                                                   <  1:2283041/93‑1 (MQ=255)

                         gCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGa                                                                >  1:7472488/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             >  1:10004812/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             <  2:9208079/93‑1 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             >  2:1589616/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             >  1:1044634/1‑93 (MQ=255)

                              actacCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATAtt                                                           >  1:3439706/1‑93 (MQ=255)

                               ctacCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATAttt                                                          >  1:8748297/1‑93 (MQ=255)

                                 acCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaa                                                        <  1:8064409/93‑1 (MQ=255)

                                  ccTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaa                                                       <  2:7235384/93‑1 (MQ=255)

                                  ccTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaa                                                       >  2:8102228/1‑93 (MQ=255)

                                  ccTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaa                                                       >  1:8550999/1‑93 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      >  1:8918557/1‑93 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      <  1:8390259/93‑1 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAAGTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      <  1:3751199/93‑1 (MQ=255)

                                    tGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAg                                                     >  2:6140612/1‑93 (MQ=255)

                                     gCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAgg                                                    >  2:1696381/1‑93 (MQ=255)

                                     gCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAgg                                                    <  1:9591741/93‑1 (MQ=255)

                                           gcagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCgg                                              <  2:1373887/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             >  2:5063720/1‑93 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             >  1:4417177/1‑93 (MQ=255)

                                             agcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGgag                                            <  1:2462739/93‑1 (MQ=255)

                                                  aaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAcc                                       <  1:7159885/93‑1 (MQ=255)

                                                  aaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAcc                                       <  2:10012287/93‑1 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGt                                     <  1:4685883/93‑1 (MQ=255)

                                                     aTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGtt                                    <  2:4436673/93‑1 (MQ=255)

                                                     aTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGtt                                    >  1:2908018/1‑93 (MQ=255)

                                                      ttGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTg                                   <  2:8018470/93‑1 (MQ=255)

                                                      ttGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTg                                   >  2:8609634/1‑93 (MQ=255)

                                                      ttGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTg                                   >  2:3362653/1‑93 (MQ=255)

                                                       tGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGa                                  <  2:2727291/93‑1 (MQ=255)

                                                        gAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAg                                 <  1:158654/93‑1 (MQ=255)

                                                        gAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAg                                 <  1:6176078/93‑1 (MQ=255)

                                                        gAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAg                                 >  1:9443278/1‑93 (MQ=255)

                                                        gAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAg                                 <  2:3101522/93‑1 (MQ=255)

                                                        gAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAg                                 <  1:140888/93‑1 (MQ=255)

                                                         aaTATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAgg                                <  1:2569297/93‑1 (MQ=255)

                                                         aaTATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAgg                                <  2:4564033/93‑1 (MQ=255)

                                                         aaTATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAgg                                >  2:2236759/1‑93 (MQ=255)

                                                          atatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGt                               <  1:6095461/93‑1 (MQ=255)

                                                          atatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGt                               >  2:2529646/1‑93 (MQ=255)

                                                           tatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc                              <  2:8835321/93‑1 (MQ=255)

                                                           tatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc                              <  1:4241503/93‑1 (MQ=255)

                                                           tatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc                              >  1:9831511/1‑93 (MQ=255)

                                                            atGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTcc                             <  1:5568105/93‑1 (MQ=255)

                                                            atGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTcc                             >  1:5305456/1‑93 (MQ=255)

                                                             tGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCa                            <  2:6827183/93‑1 (MQ=255)

                                                             tGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCa                            <  2:7305162/93‑1 (MQ=255)

                                                             tGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCa                            <  2:4725000/93‑1 (MQ=255)

                                                             tGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCa                            <  2:5002183/93‑1 (MQ=255)

                                                               cgcCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACg                          >  1:6687205/1‑93 (MQ=255)

                                                                 ccGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGaa                        <  2:5198930/93‑1 (MQ=255)

                                                                  cGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAg                       >  1:3091580/1‑93 (MQ=255)

                                                                  cGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAg                       <  2:6641756/93‑1 (MQ=255)

                                                                  cGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAg                       >  2:3722980/1‑93 (MQ=255)

                                                                   gAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAgg                      >  2:4318216/1‑93 (MQ=255)

                                                                   gAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAgg                      >  1:1281710/1‑93 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     >  2:2814571/1‑93 (MQ=255)

                                                                     aaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGc                    <  1:5417487/93‑1 (MQ=255)

                                                                     aaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGc                    <  1:403120/93‑1 (MQ=255)

                                                                       gagaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGTCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCaa                  >  2:4002652/1‑93 (MQ=255)

                                                                       gagaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCaa                  >  2:4950838/1‑93 (MQ=255)

                                                                         gaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAAt                >  1:2081919/1‑93 (MQ=255)

                                                                         gaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAAt                >  2:306178/1‑93 (MQ=255)

                                                                            aTTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGt             >  1:1836043/1‑93 (MQ=255)

                                                                             ttACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGtt            >  1:4897276/1‑93 (MQ=255)

                                                                               aCAGGTAGTGGGATGTTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAt          >  1:9336439/1‑93 (MQ=255)

                                                                               aCAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAt          <  2:8420977/93‑1 (MQ=255)

                                                                                   gTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGc      <  1:574738/93‑1 (MQ=255)

                                                                                      gTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGat   >  2:5395777/1‑93 (MQ=255)

                                                                                       tGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGata  >  2:7132744/1‑93 (MQ=255)

                                                                                       tGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGata  <  1:2746708/93‑1 (MQ=255)

                                                                                       tGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGata  <  2:5707758/93‑1 (MQ=255)

                                                                                       tGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGata  >  1:1190193/1‑93 (MQ=255)

                                                                                       tGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGATGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGata  >  1:6378902/1‑93 (MQ=255)

CCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATA  >  NC_000913/3223541‑3223720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`