BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,228,614	G→A	A696T (GCT→ACT)	ygjK →	putative isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,228,614	0	G	A	99.7%	1265.4 / ‑6.0	358	A696T (GCT→ACT)	ygjK	putative isomerase
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (188/168); total (190/168)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 375 total aligned reads displayed.

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGT  >  NC_000913/3228524‑3228703

gCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACt                                                                                         <  2:1119315/93‑1 (MQ=255)

  cAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACttt                                                                                       >  2:7121807/1‑93 (MQ=255)

  cAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACttt                                                                                       <  2:6331089/93‑1 (MQ=255)

   aaTGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACtttt                                                                                      <  2:9853317/93‑1 (MQ=255)

     tGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTgg                                                                                    >  2:4047577/1‑93 (MQ=255)

        cGACGCGCTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGgcgc                                                                                 <  2:8233893/93‑1 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                              <  1:2299515/93‑1 (MQ=255)

           cgcgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGa                                                                              <  1:8339492/93‑1 (MQ=255)

             cgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGata                                                                            <  2:1711003/93‑1 (MQ=255)

             cgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGata                                                                            >  1:9905080/1‑93 (MQ=255)

             cgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGata                                                                            <  1:9036476/93‑1 (MQ=255)

             cgGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGata                                                                            <  1:4504187/93‑1 (MQ=255)

              ggtggtGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGatat                                                                           <  1:9403897/93‑1 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGGGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                          >  2:3182490/1‑93 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                          <  2:3266813/93‑1 (MQ=255)

               gtggtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATc                                                                          <  1:9252798/93‑1 (MQ=255)

                  gtgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTAc                                                                       >  1:8171788/1‑93 (MQ=255)

                   tgAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACt                                                                      <  1:3292151/93‑1 (MQ=255)

                    gAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTg                                                                     >  1:8722032/1‑93 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGCACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                    >  1:4390294/1‑93 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                    >  2:7429753/1‑93 (MQ=255)

                     aaGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTgg                                                                    >  1:7965975/1‑93 (MQ=255)

                        gTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGt                                                                 >  2:3927854/1‑93 (MQ=255)

                        gTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGt                                                                 >  2:2251341/1‑93 (MQ=255)

                          taTGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTgg                                                               <  1:2137849/92‑1 (MQ=255)

                          gATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTgg                                                               <  1:6434272/93‑1 (MQ=255)

                           aTGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTggg                                                              >  1:6243865/1‑93 (MQ=255)

                           aTGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGAGTTAACCAATCCGCCTTTTGGCGCTGATATCTACTGGCGTggg                                                              >  1:2378927/1‑93 (MQ=255)

                            tGCTCGATCGTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGgc                                                             <  2:6147235/93‑1 (MQ=255)

                             gCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGgcg                                                            <  1:8864688/93‑1 (MQ=255)

                             gCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGAGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGgcg                                                            >  1:1493273/1‑93 (MQ=255)

                              cTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGgcgc                                                           >  1:3721999/1‑93 (MQ=255)

                               tCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGcgcg                                                          <  2:2960080/93‑1 (MQ=255)

                                cGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGt                                                         >  1:5456151/1‑93 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                        <  1:3616697/93‑1 (MQ=255)

                                 gATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                        <  1:6569441/93‑1 (MQ=255)

                                 gATCCTAAAGAGGTCAACACCGTTGTCACGCTGGGAACGGCGGCGGTAACCGATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTa                                                        <  1:614391/93‑1 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                       >  1:187121/1‑93 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                       >  2:1052965/1‑93 (MQ=255)

                                  aTCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTAt                                                       >  1:8447853/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                      >  2:6058159/1‑93 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                      <  2:2464803/93‑1 (MQ=255)

                                   tCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATg                                                      <  2:6812290/93‑1 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                    <  1:5572162/93‑1 (MQ=255)

                                     cTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATggg                                                    <  2:8042635/93‑1 (MQ=255)

                                      tAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGt                                                   <  2:595625/93‑1 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTCTGGGTg                                                  >  2:9172594/1‑93 (MQ=255)

                                       aaaGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTg                                                  >  2:4592806/1‑93 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                <  1:6377571/93‑1 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                <  2:52353/93‑1 (MQ=255)

                                         agagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGa                                                <  1:5256733/93‑1 (MQ=255)

                                          gagTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGAt                                               >  1:5746463/1‑93 (MQ=255)

                                            gTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCa                                             <  1:3077951/93‑1 (MQ=255)

                                             ttCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAg                                            >  1:4852470/1‑93 (MQ=255)

                                              tCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGt                                           >  2:4366144/1‑93 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                         >  2:8654164/1‑93 (MQ=255)

                                                aaCACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTc                                         >  2:1323215/1‑93 (MQ=255)

                                                 acacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCt                                        >  1:1188413/1‑93 (MQ=255)

                                                 acacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCt                                        >  2:5893152/1‑93 (MQ=255)

                                                 acacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCt                                        >  1:1646185/1‑93 (MQ=255)

                                                 acacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCt                                        <  2:7624558/93‑1 (MQ=255)

                                                  cacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTg                                       <  2:8864280/93‑1 (MQ=255)

                                                  cacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTg                                       <  1:7752519/93‑1 (MQ=255)

                                                  cacCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACGGGCGTGGGCGCGGATGGGTGGATCAGTTCTg                                       >  1:292719/1‑93 (MQ=255)

                                                   acCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTgg                                      >  2:6960370/1‑93 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                     >  2:1253808/1‑93 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                     >  2:4951690/1‑93 (MQ=255)

                                                    ccTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGt                                     <  1:1030278/93‑1 (MQ=255)

                                                     cTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGtt                                    >  1:2437196/1‑93 (MQ=255)

                                                          tCCCGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTc                               <  1:506292/93‑1 (MQ=255)

                                                            ccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGGTCAGTTCTGGTTTGGTCTg                             >  2:5047193/1‑93 (MQ=255)

                                                            ccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTg                             >  1:7372539/1‑93 (MQ=255)

                                                            ccGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTg                             >  1:708142/1‑93 (MQ=255)

                                                             cGCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGa                            >  2:4809789/1‑93 (MQ=255)

                                                              gCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaa                           >  1:4025189/1‑93 (MQ=255)

                                                              gCTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaa                           >  1:5095947/1‑93 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaaa                          <  1:3965347/93‑1 (MQ=255)

                                                               cTGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGaaa                          >  2:2538608/1‑93 (MQ=255)

                                                                tGGGAACGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAg                         >  1:6943586/1‑93 (MQ=255)

                                                                    aaCGGCGGCGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGAt                     >  2:723699/1‑93 (MQ=255)

                                                                    aaCGGCGGAGTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGCATGGGAGGCTCAGTTCTGGTTTGGTCTGACAGGGAt                     >  1:2787926/1‑93 (MQ=255)

                                                                        gcggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAg                 <  2:8424150/93‑1 (MQ=255)

                                                                        gcggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAg                 >  2:260457/1‑93 (MQ=255)

                                                                          ggcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGCGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCg               >  2:9653024/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt              >  2:6387441/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt              >  1:420091/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt              >  1:2620053/1‑93 (MQ=255)

                                                                           gcgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGt              <  1:7605144/93‑1 (MQ=255)

                                                                            cgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGtt             <  2:353279/93‑1 (MQ=255)

                                                                            cgTTAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGtt             <  2:7548941/93‑1 (MQ=255)

                                                                            cgTTAACCAAGCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGtt             >  2:6550542/1‑93 (MQ=255)

                                                                               tAACCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACg          >  1:7860666/1‑93 (MQ=255)

                                                                                aaCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACgg         >  1:1735853/1‑93 (MQ=255)

                                                                                 aCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGt        >  2:1599868/1‑93 (MQ=255)

                                                                                 aCCAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGt        <  2:2059261/93‑1 (MQ=255)

                                                                                   cAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTa      <  2:7040804/93‑1 (MQ=255)

                                                                                   cAATCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTa      >  2:5689590/1‑93 (MQ=255)

                                                                                    aaTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTAt     >  1:7908884/1‑93 (MQ=255)

                                                                                     aTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATc    <  2:7882287/93‑1 (MQ=255)

                                                                                     aTCCGACTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATc    <  1:3125416/93‑1 (MQ=255)

GCCAATGCCGACGCGGTGGTGAAGGTGATGCTCGATCCTAAAGAGTTCAACACCTTTGTCCCGCTGGGAACGGCGGCGTTAACCAATCCGGCTTTTGGCGCTGATATCTACTGGCGTGGGCGCGTATGGGTGGATCAGTTCTGGTTTGGTCTGAAAGGGATGGAGCGTTACGGTTATCGT  >  NC_000913/3228524‑3228703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`