BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,253,781	T→C	E171G (GAG→GGG)	yhaNM ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,253,781	0	T	C	99.7%	1011.9 / ‑4.9	316	E171G (GAG→GGG)	yhaNM	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (158/157); minor base A (1/0); total (159/157)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.74e-01

Only 100 of 335 total aligned reads displayed.

ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253690‑3253870

aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCccct                                                                                         <  1:3260315/93‑1 (MQ=255)

aTCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGCAGACTCTTGCTCC‑CCccct                                                                                         >  1:1076719/1‑93 (MQ=255)

  cgccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGc                                                                                       >  1:7601714/1‑93 (MQ=255)

   gccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAATGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGcc                                                                                      >  1:6084229/1‑93 (MQ=255)

    ccgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCca                                                                                     <  1:2622995/93‑1 (MQ=255)

     cgcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCcac                                                                                    >  1:6890879/1‑93 (MQ=255)

      gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCcaca                                                                                   >  2:6485437/1‑93 (MQ=255)

      gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCcaca                                                                                   <  1:2197234/93‑1 (MQ=255)

      gcAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCcaca                                                                                   >  1:2025493/1‑93 (MQ=255)

        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCcacaca                                                                                 >  2:3905480/1‑93 (MQ=255)

        aaaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCcacaca                                                                                 >  2:5921280/1‑93 (MQ=255)

         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCacacac                                                                                >  2:4350842/1‑93 (MQ=255)

         aaCGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCG‑CCGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGATAT‑CCCCCTGTCgcacac                                                                                >  2:4166290/1‑93 (MQ=255)

           cGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGc                                                                              >  1:6017715/1‑93 (MQ=255)

            gggACTTTGTTGACGAACTTCAGTATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGcc                                                                             <  2:4016365/93‑1 (MQ=255)

            gggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGcc                                                                             <  1:6943677/93‑1 (MQ=255)

             ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCct                                                                            >  2:3942182/1‑93 (MQ=255)

             ggACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCct                                                                            >  2:3317611/1‑93 (MQ=255)

              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCctg                                                                           >  2:1246664/1‑93 (MQ=255)

              gACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCctg                                                                           >  2:1937071/1‑93 (MQ=255)

               aCTTCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCctgc                                                                          <  1:10005629/93‑1 (MQ=255)

                 ttCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCctgctg                                                                        <  1:4493902/93‑1 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTgg                                                                       >  2:8710719/1‑93 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTgg                                                                       <  2:7412908/93‑1 (MQ=255)

                  tCATTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTgg                                                                       <  1:5247106/93‑1 (MQ=255)

                    aTTGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGt                                                                     >  2:1550316/1‑93 (MQ=255)

                     ttGACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTa                                                                    <  1:338961/93‑1 (MQ=255)

                       gACGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTaaa                                                                  <  2:2663420/93‑1 (MQ=255)

                        aCGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAac                                                                 >  1:705869/1‑93 (MQ=255)

                        aCGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAac                                                                 >  2:1999817/1‑93 (MQ=255)

                        aCGAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAac                                                                 <  1:3525815/93‑1 (MQ=255)

                          gAACTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacac                                                               <  1:8408671/93‑1 (MQ=255)

                           aaCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacacc                                                              >  2:6118994/1‑93 (MQ=255)

                            aCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacacca                                                             <  1:1873478/93‑1 (MQ=255)

                            aCTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacacca                                                             >  1:6703184/1‑93 (MQ=255)

                             cTTCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacaccac                                                            <  1:1546598/93‑1 (MQ=255)

                              ttCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacaccaca                                                           <  1:4973244/93‑1 (MQ=255)

                               tCAGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAacaccacac                                                          >  1:7357494/1‑93 (MQ=255)

                                 aGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAAcaccacacca                                                        >  2:9537664/1‑93 (MQ=255)

                                 aGGATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAAcaccacacca                                                        >  1:1662319/1‑93 (MQ=255)

                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCGCCCCCCTGCCACACACGCCTGCTGGGTAAAcaccacacca                                                        >  2:8479936/1‑93 (MQ=255)

                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       <  2:5841496/93‑1 (MQ=255)

                                  ggATCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       >  2:7989584/1‑93 (MQ=255)

                                  ggATCTCAACCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCAt                                                       <  1:8012118/93‑1 (MQ=255)

                                    aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     <  1:471582/93‑1 (MQ=255)

                                    aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     <  2:2997492/93‑1 (MQ=255)

                                    aTCTCAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCg                                                     >  2:2924466/1‑93 (MQ=255)

                                     tctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCACCTGCCACACACGCCTGCTGGGTCAACACCACAACAtaga                                                    >  1:7836788/1‑90 (MQ=255)

                                      ctcAGCCAGCGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTg                                                   >  2:5187336/1‑93 (MQ=255)

                                        cagccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCg                                                 >  2:9217285/1‑93 (MQ=255)

                                          gccagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtc                                               <  1:9273964/93‑1 (MQ=255)

                                            cagcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGtctc                                             >  1:5109106/1‑93 (MQ=255)

                                             agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                            >  2:10024096/1‑93 (MQ=255)

                                             agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                            >  2:1457402/1‑93 (MQ=255)

                                             agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                            >  2:6368813/1‑93 (MQ=255)

                                             agcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCg                                            >  1:5450199/1‑93 (MQ=255)

                                              gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           >  1:2520567/1‑93 (MQ=255)

                                              gcGTC‑GTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGa                                           >  2:7749993/1‑93 (MQ=255)

                                                 gtcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGata                                         <  1:9729321/90‑1 (MQ=255)

                                                 gtcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGata                                         <  1:3808071/90‑1 (MQ=255)

                                                 gtcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGata                                         <  2:8401802/90‑1 (MQ=255)

                                                  tcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGatat                                        <  2:4943019/91‑1 (MQ=255)

                                                  tcgTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGatat                                        >  1:5626185/3‑93 (MQ=255)

                                                     ttCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGca                                     <  1:8211009/93‑1 (MQ=255)

                                                     ttCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGca                                     >  2:6040680/1‑93 (MQ=255)

                                                       cTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcaca                                   >  2:612978/1‑93 (MQ=255)

                                                       cTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGcaca                                   <  2:826518/93‑1 (MQ=255)

                                                        tGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  >  1:8781779/1‑93 (MQ=255)

                                                        tGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACaa                                  >  2:1670878/1‑93 (MQ=255)

                                                         ggAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAAt                                 >  2:7354948/1‑93 (MQ=255)

                                                            aaGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGtt                              <  1:9528317/93‑1 (MQ=255)

                                                            aaGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGtt                              <  2:907636/93‑1 (MQ=255)

                                                             aGCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTg                             <  1:5649093/93‑1 (MQ=255)

                                                              gCACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTgg                            <  1:9443885/93‑1 (MQ=255)

                                                               gaCCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGt                           <  2:9330936/92‑1 (MQ=255)

                                                               cACCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGt                           <  2:6221905/93‑1 (MQ=255)

                                                                cccGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          <  2:4837742/92‑1 (MQ=255)

                                                                cccGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          <  1:3706473/92‑1 (MQ=255)

                                                                aCCGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTa                          <  2:4507681/93‑1 (MQ=255)

                                                                 ccGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTAt                         >  1:8633368/1‑93 (MQ=255)

                                                                  cGTCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATg                        <  1:4343430/93‑1 (MQ=255)

                                                                    tCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      <  2:4775002/93‑1 (MQ=255)

                                                                    tCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      >  2:7124438/1‑93 (MQ=255)

                                                                    tCAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      <  2:3502376/93‑1 (MQ=255)

                                                                    ccAGCGGAGACCCTTTCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGcc                      <  1:2987716/92‑1 (MQ=255)

                                                                     cAGCGGAGCCTCTTCCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGccc                     <  2:7167217/93‑1 (MQ=255)

                                                                     cAGCGGAGACTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGccc                     <  1:1408459/93‑1 (MQ=255)

                                                                           agcctctTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgac               <  1:6399446/90‑1 (MQ=255)

                                                                           agaCTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgac               >  2:6808970/1‑93 (MQ=255)

                                                                            gaCTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacg              >  1:4065185/1‑93 (MQ=255)

                                                                             aCTCTTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCcgacga             <  1:6574146/93‑1 (MQ=255)

                                                                              ccctTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAACCACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGAt            <  1:4030639/91‑1 (MQ=255)

                                                                                ctTGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATgg          >  1:588982/1‑93 (MQ=255)

                                                                                 ttGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGt         <  2:7601713/93‑1 (MQ=255)

                                                                                  tGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTg        >  1:1098386/1‑93 (MQ=255)

                                                                                  tGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTg        <  2:7479320/93‑1 (MQ=255)

                                                                                  tGCTCG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCACCGACGATGGTg        >  2:7084572/1‑93 (MQ=255)

                                                                                      cG‑CCCCCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacac    <  1:3881140/93‑1 (MQ=255)

                                                                                        cccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacaca   <  2:5660601/92‑1 (MQ=255)

                                                                                         ccccCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGacacac  >  2:2204506/1‑93 (MQ=255)

ATCGCCGCAAACGGGACTTCATTGACGAACTTCAGGATCTCAGCCAGCGTCGGTTCTGGAAAGCACCGTCAGCGGAGACTCTTGCTCG‑CCCTCTGCCACACACGCCTGCTGGGTAAACACCACACCATCGTGCGTCTCGATATGCACAATGTTGGTATGCCCGCCGACGATGGTGACACAC  >  NC_000913/3253690‑3253870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`