BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,300,625	G→A	V172I (GTC→ATC)	yhbV →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,300,625	0	G	A	99.7%	1245.4 / NA	346	V172I (GTC→ATC)	yhbV	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (165/180); total (165/181)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

TGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACC  >  NC_000913/3300535‑3300714

tgtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATc                                                                                         >  1:7741026/1‑93 (MQ=255)

tgtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATc                                                                                         >  2:7496517/1‑93 (MQ=255)

 gtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATcc                                                                                        <  2:2954212/93‑1 (MQ=255)

 gtATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGGGGAAATcc                                                                                        >  2:7593893/1‑93 (MQ=255)

  tATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCt                                                                                       >  1:4711522/1‑93 (MQ=255)

  tATGCCAGTGAAGCTTTCCCGCGTGTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTACCCAGTTTGAAGTGGAAATCCt                                                                                       <  2:5520896/93‑1 (MQ=255)

    tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGGAGCTTCTTCATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTCAAGAGGAAAACCTGa                                                                                     >  2:8501449/1‑93 (MQ=255)

    tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAGATCCTGa                                                                                     >  1:5574651/1‑93 (MQ=255)

    tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGa                                                                                     <  2:8296600/93‑1 (MQ=255)

    tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGa                                                                                     <  1:3437791/93‑1 (MQ=255)

    tGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGa                                                                                     >  2:5920644/1‑93 (MQ=255)

     gCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAg                                                                                    <  2:8820939/93‑1 (MQ=255)

      ccAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGc                                                                                   <  1:5690962/93‑1 (MQ=255)

      ccAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGCGCGATGAGCTGGGCATTCGTAACCAGTTTTAAGTGGAAATCCTCAGc                                                                                   >  1:9645421/1‑93 (MQ=255)

        aGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTa                                                                                 <  1:9792375/93‑1 (MQ=255)

         gTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTAc                                                                                >  1:372395/1‑93 (MQ=255)

         gTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTAc                                                                                >  2:352796/1‑93 (MQ=255)

         gTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTAc                                                                                >  1:4990432/1‑93 (MQ=255)

          tGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACg                                                                               >  2:463555/1‑93 (MQ=255)

          tGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACg                                                                               >  1:1702009/1‑93 (MQ=255)

          tGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACg                                                                               >  1:1374662/1‑93 (MQ=255)

           ggAGCTTTCCCGCGACCGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACgg                                                                              >  1:7476149/1‑93 (MQ=255)

              gCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCa                                                                           <  1:2241984/93‑1 (MQ=255)

              gCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCa                                                                           <  1:6901737/93‑1 (MQ=255)

               cTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGACATCCTGAGCTACGGTCAt                                                                          >  1:1915328/1‑93 (MQ=255)

               cTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCAt                                                                          <  2:282249/93‑1 (MQ=255)

               cTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCAt                                                                          <  1:6953895/93‑1 (MQ=255)

                tttCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATc                                                                         >  2:9308898/1‑93 (MQ=255)

                tttCCCGCGACTGGCTGGTGAATCTGCTCAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATc                                                                         <  1:1018108/93‑1 (MQ=255)

                 ttCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCt                                                                        >  1:2336615/1‑93 (MQ=255)

                        gACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTg                                                                 >  1:4786853/1‑93 (MQ=255)

                         aCTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTgg                                                                >  2:5467855/1‑93 (MQ=255)

                         aCTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTgg                                                                >  1:3920014/1‑93 (MQ=255)

                          ctggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGc                                                               >  1:1537515/1‑93 (MQ=255)

                          ctggctggTGAATCTGCTTAATCAGAGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACCGTCATCTGCCGCTGGc                                                               >  2:3652734/1‑93 (MQ=255)

                           tggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCCGTTTGACGTGGAAATCCTGCGCTACGGTCCTCTGCCGCTGGcc                                                              >  1:6750678/1‑93 (MQ=255)

                           tggctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGcc                                                              >  1:7048930/1‑93 (MQ=255)

                             gctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTa                                                            <  2:5317885/93‑1 (MQ=255)

                              ctggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTAc                                                           <  2:700021/93‑1 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         >  1:854613/1‑93 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         <  1:7272308/93‑1 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         >  2:511254/1‑93 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         <  1:8358033/93‑1 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         <  2:4269466/93‑1 (MQ=255)

                                ggTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTAAGCTACGGTCATCTGCCGCTGGCCTACTc                                                         <  2:6217161/93‑1 (MQ=255)

                                 gTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTcc                                                        >  1:5837452/1‑93 (MQ=255)

                                 gTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTcc                                                        <  1:1847836/93‑1 (MQ=255)

                                    aaTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcc                                                     <  1:8678345/93‑1 (MQ=255)

                                    aaTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcc                                                     <  1:4333001/93‑1 (MQ=255)

                                    aaTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcc                                                     <  2:3698381/93‑1 (MQ=255)

                                     aTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgccc                                                    >  1:7380562/1‑93 (MQ=255)

                                     aTCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgccc                                                    >  2:2428033/1‑93 (MQ=255)

                                      tCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcccg                                                   >  1:795148/1‑93 (MQ=255)

                                      tCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcccg                                                   >  1:5300827/1‑93 (MQ=255)

                                       cTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTccgcccgc                                                  <  2:9172027/93‑1 (MQ=255)

                                        tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                                 >  2:3912683/1‑93 (MQ=255)

                                        tGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgct                                                 <  1:9105395/93‑1 (MQ=255)

                                         gCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctg                                                >  1:9240263/1‑93 (MQ=255)

                                          cTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgc                                               >  2:6800499/1‑93 (MQ=255)

                                          cTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgc                                               <  2:2555971/93‑1 (MQ=255)

                                           ttAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCgctgct                                              >  1:6120975/1‑93 (MQ=255)

                                            tAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCtt                                             >  2:2104282/1‑93 (MQ=255)

                                             aaTCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCttt                                            <  1:6516285/93‑1 (MQ=255)

                                               tCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTAc                                          >  2:1583478/1‑93 (MQ=255)

                                                  gTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACcgc                                       >  2:7956981/1‑93 (MQ=255)

                                                      gATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGt                                   <  2:903792/93‑1 (MQ=255)

                                                      gATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGt                                   <  1:5118524/93‑1 (MQ=255)

                                                      gATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGt                                   <  2:436510/93‑1 (MQ=255)

                                                       aTGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGtt                                  >  1:8409926/1‑93 (MQ=255)

                                                        tGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTc                                 <  2:4171204/93‑1 (MQ=255)

                                                         gAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                                >  2:4986276/1‑93 (MQ=255)

                                                         gAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCg                                <  1:1111497/93‑1 (MQ=255)

                                                           gCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGa                              >  2:139246/1‑93 (MQ=255)

                                                           gCTGGGCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGa                              <  2:1116488/93‑1 (MQ=255)

                                                              gggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGa                           <  2:5379541/93‑1 (MQ=255)

                                                               ggCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAc                          <  2:7313471/93‑1 (MQ=255)

                                                                gCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAcc                         >  2:1349217/1‑93 (MQ=255)

                                                                gCATTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGAcc                         >  1:6435421/1‑93 (MQ=255)

                                                                  aTTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGc                       >  1:2503082/1‑93 (MQ=255)

                                                                  aTTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGc                       <  2:6396016/93‑1 (MQ=255)

                                                                  aTTCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTGTACCGCGCGTTCGGAAGACCGc                       >  2:1768640/1‑93 (MQ=255)

                                                                   ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                      >  1:2846377/1‑93 (MQ=255)

                                                                   ttCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGcc                      <  2:1949657/93‑1 (MQ=255)

                                                                    tCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGccc                     <  2:7728213/93‑1 (MQ=255)

                                                                    tCGTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGccc                     <  2:2463749/93‑1 (MQ=255)

                                                                    tCGTAACCAGTTTGAAGTGGAAACCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGccc                     <  2:5716993/93‑1 (MQ=255)

                                                                      gTAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGa                   <  2:2705872/93‑1 (MQ=255)

                                                                       tAACCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGaa                  >  2:4743002/1‑93 (MQ=255)

                                                                         aCCAGTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAg                >  2:1452093/1‑93 (MQ=255)

                                                                             gTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa            <  2:9644773/93‑1 (MQ=255)

                                                                             gTTTGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGa            <  2:4575259/93‑1 (MQ=255)

                                                                              tttGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAg           <  1:4061000/93‑1 (MQ=255)

                                                                                tGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtg         <  2:8610380/93‑1 (MQ=255)

                                                                                tGAAGTGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAgtg         <  2:9406279/93‑1 (MQ=255)

                                                                                     tGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGaaa    >  2:7213934/1‑93 (MQ=255)

                                                                                     tGGAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGaaa    >  2:3316453/1‑93 (MQ=255)

                                                                                      ggAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAAc   <  1:9167994/93‑1 (MQ=255)

                                                                                       gAATTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAAcc  <  2:4011141/93‑1 (MQ=255)

                                                                                       gAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAAcc  <  1:8818785/93‑1 (MQ=255)

                                                                                       gAAATCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAAcc  <  2:1905736/93‑1 (MQ=255)

TGTATGCCAGTGGAGCTTTCCCGCGACTGGCTGGTGAATCTGCTTAATCAGTGCGATGAGCTGGGCATTCGTAACCAGTTTGAAGTGGAAGTCCTGAGCTACGGTCATCTGCCGCTGGCCTACTCCGCCCGCTGCTTTACCGCGCGTTCGGAAGACCGCCCGAAAGATGAGTGTGAAACC  >  NC_000913/3300535‑3300714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`