Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,337,699 T→C Q3R (CAG→CGG)  yrbF ← putative ATP‑binding component of a transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,337,6990TC99.6% 1729.6 / ‑6.1 539Q3R (CAG→CGG) yrbFputative ATP‑binding component of a transport system
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (307/230);  total (308/231)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 555 total aligned reads displayed.

CGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACC  >  NC_000913/3337609‑3337788
                                                                                          |                                                                                         
cgcgGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGc                                                                                         >  1:1391496/1‑93 (MQ=255)
cgcgGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGc                                                                                         <  1:1754751/93‑1 (MQ=255)
cgcgGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGc                                                                                         <  2:6723594/93‑1 (MQ=255)
  cgGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTc                                                                                       <  2:2864607/93‑1 (MQ=255)
     cACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCAt                                                                                    <  2:8149065/93‑1 (MQ=255)
     cACGGTCAGGGAAATATTATCGAAGATGCAGCGATCGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCAt                                                                                    >  1:6888706/1‑93 (MQ=255)
        ggTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAAt                                                                                 >  1:4295589/1‑93 (MQ=255)
         gTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAACTTCGCCACAGACCGCTCCATAAtt                                                                                >  2:5269807/1‑93 (MQ=255)
           cAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCa                                                                              >  2:7531886/1‑93 (MQ=255)
             gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAcc                                                                            >  1:4572658/1‑93 (MQ=255)
             gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAcc                                                                            <  1:3918876/93‑1 (MQ=255)
             gggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAcc                                                                            >  2:4581495/1‑93 (MQ=255)
              ggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAccc                                                                           <  2:9887764/93‑1 (MQ=255)
              ggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAccc                                                                           >  1:4996835/1‑93 (MQ=255)
              ggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAccc                                                                           <  1:2636386/93‑1 (MQ=255)
              ggAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCAccc                                                                           >  2:6109726/1‑93 (MQ=255)
               gAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCt                                                                          >  2:1463318/1‑93 (MQ=255)
               gAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCt                                                                          <  2:8035223/93‑1 (MQ=255)
                aaaTATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGAACGCTCCATAATTCACCCtt                                                                         >  2:4159474/1‑93 (MQ=255)
                 aaTATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTc                                                                        >  2:7276300/1‑93 (MQ=255)
                 aaTATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTc                                                                        <  1:4485553/93‑1 (MQ=255)
                   tattatCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGt                                                                      >  1:5672789/1‑93 (MQ=255)
                     ttatCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCt                                                                    <  2:6901027/93‑1 (MQ=255)
                       atCAAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTg                                                                  <  1:8068307/93‑1 (MQ=255)
                         cGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCg                                                                >  1:7840807/1‑93 (MQ=255)
                          gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGt                                                               >  1:4137809/1‑93 (MQ=255)
                          gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGt                                                               <  1:5492388/93‑1 (MQ=255)
                          gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGt                                                               >  2:1151518/1‑93 (MQ=255)
                          gAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGt                                                               >  1:8937012/1‑93 (MQ=255)
                           aaGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGtt                                                              >  2:953258/1‑93 (MQ=255)
                              aTGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGAt                                                           >  1:1868253/1‑93 (MQ=255)
                               tGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGAtt                                                          >  2:901018/1‑93 (MQ=255)
                                 cAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGAtttt                                                        <  1:3788792/93‑1 (MQ=255)
                                  aGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTc                                                       <  1:5104409/93‑1 (MQ=255)
                                     gATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTaa                                                    >  1:9750136/1‑93 (MQ=255)
                                      aTTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg                                                   <  1:8500492/93‑1 (MQ=255)
                                      aTTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAg                                                   >  1:3134833/1‑93 (MQ=255)
                                       ttGCAACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGc                                                  >  2:1754788/1‑93 (MQ=255)
                                        tGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCa                                                 <  1:8368044/93‑1 (MQ=255)
                                         gCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCAt                                                >  1:2787796/1‑93 (MQ=255)
                                           cACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATgg                                              <  2:3283267/93‑1 (MQ=255)
                                           cACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATgg                                              <  1:9095611/93‑1 (MQ=255)
                                           cACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATgg                                              >  1:4824968/1‑93 (MQ=255)
                                           cACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATgg                                              <  2:6416344/93‑1 (MQ=255)
                                             cgcgTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGCTTTTCTAAGCATGgcg                                            >  1:2568507/1‑93 (MQ=255)
                                              gcgTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGgcgc                                           >  1:2467667/1‑93 (MQ=255)
                                                gTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTc                                         <  1:1094913/93‑1 (MQ=255)
                                                gTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTc                                         <  2:4756569/93‑1 (MQ=255)
                                                 tAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCa                                        >  1:641091/1‑93 (MQ=255)
                                                   aaaCTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAt                                      >  1:7391931/1‑93 (MQ=255)
                                                    aaCTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAtt                                     <  1:4367700/93‑1 (MQ=255)
                                                    aaCTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAtt                                     <  1:2206767/93‑1 (MQ=255)
                                                    aaCTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAtt                                     >  2:9670334/1‑93 (MQ=255)
                                                     aCTGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAAttt                                    >  2:6581149/1‑93 (MQ=255)
                                                       tGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTaa                                  <  1:9296573/93‑1 (MQ=255)
                                                       tGACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTaa                                  >  2:161003/1‑93 (MQ=255)
                                                        gACATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAc                                 >  2:5355676/1‑93 (MQ=255)
                                                         aCATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAcc                                <  2:5618583/93‑1 (MQ=255)
                                                         aCATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAAcc                                <  1:1670586/93‑1 (MQ=255)
                                                          cATCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCt                               >  2:3715757/1‑93 (MQ=255)
                                                           aTCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCtt                              <  1:3356373/93‑1 (MQ=255)
                                                            tCGCGCATATCGACTAAATTCGCCTCAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGCTTTTCTAAGCATGGCGCTCAATTTAACCTTg                             >  1:1556406/1‑93 (MQ=255)
                                                            tCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTg                             <  2:5020476/93‑1 (MQ=255)
                                                            tCGCGCATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTg                             >  1:8781908/1‑93 (MQ=255)
                                                              gcgcATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGCTTTTCTACGCATGGCGCTCAATTTCACCTTgca                           >  1:3260509/1‑91 (MQ=255)
                                                               cgcATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAc                          >  1:7085329/1‑93 (MQ=255)
                                                                gcATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAcc                         >  2:2207214/1‑93 (MQ=255)
                                                                gcATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAcc                         >  1:6118700/1‑93 (MQ=255)
                                                                 cATATCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAAccc                        >  2:7119866/1‑93 (MQ=255)
                                                                   tatCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCaa                      >  1:10018783/1‑93 (MQ=255)
                                                                   tatCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCaa                      >  1:3568251/1‑93 (MQ=255)
                                                                   tatCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCaa                      >  2:9208389/1‑93 (MQ=255)
                                                                   tatCGACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTAAATTTAACCTTGAACCCaa                      >  2:3844378/1‑93 (MQ=255)
                                                                   tatCAACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCaa                      >  1:9936038/1‑93 (MQ=255)
                                                                     tgcaCTAAAGTCGCCACAGACCGCTTCATAATTCAGCCTTCGTCTTGCGTTGATTTTCTAAGCAGGGCGCTCAATTTAACCTTGAACCCAACa                    <  2:8320027/90‑1 (MQ=255)
                                                                       gACTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACata                  <  2:3132994/93‑1 (MQ=255)
                                                                        aCTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACatat                 >  1:9569713/1‑93 (MQ=255)
                                                                         cTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAtt                <  2:7769733/93‑1 (MQ=255)
                                                                         cTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAtt                <  2:87953/93‑1 (MQ=255)
                                                                         cTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAtt                >  2:2079497/1‑93 (MQ=255)
                                                                         cTAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAtt                <  1:818547/93‑1 (MQ=255)
                                                                          tAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAttt               >  2:270821/1‑93 (MQ=255)
                                                                          tAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAttt               >  2:2365324/1‑93 (MQ=255)
                                                                          tAAATTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATAttt               <  2:1972249/93‑1 (MQ=255)
                                                                           aaaTTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTa              <  2:4471771/93‑1 (MQ=255)
                                                                            aaTTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTAc             <  1:6689183/93‑1 (MQ=255)
                                                                             aTTCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACa            <  2:5865686/93‑1 (MQ=255)
                                                                              ttCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAg           <  2:1302665/93‑1 (MQ=255)
                                                                               tCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGa          <  1:9428726/93‑1 (MQ=255)
                                                                               tCGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGa          >  1:5814866/1‑93 (MQ=255)
                                                                                cGCCACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGaa         <  2:6316551/93‑1 (MQ=255)
                                                                                  ccACAGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAata       <  1:7672903/93‑1 (MQ=255)
                                                                                   cacaGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAatat      >  2:852543/1‑93 (MQ=255)
                                                                                    acaGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATAtt     <  2:3005071/93‑1 (MQ=255)
                                                                                    acaGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATAtt     >  2:9327216/1‑93 (MQ=255)
                                                                                     caGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTa    <  2:4010583/93‑1 (MQ=255)
                                                                                      aGACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTAc   <  1:1273915/93‑1 (MQ=255)
                                                                                       gACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTAcc  >  1:2966706/1‑93 (MQ=255)
                                                                                       gACCGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTAcc  >  2:3548343/1‑93 (MQ=255)
                                                                                       gACCGCTCCATAATTCACCCCTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTAcc  <  1:7246655/93‑1 (MQ=255)
                                                                                          |                                                                                         
CGCGGCACGGTCAGGGAAATATTATCGAAGATGCAGCGATTGCCACGCGTAAAACTGACATCGCGCATATCGACTAAATTCGCCACAGACTGCTCCATAATTCACCCTTCGTCTTGCGTTGATTTTCTAAGCATGGCGCTCAATTTAACCTTGAACCCAACATATTTACAGAATATTACC  >  NC_000913/3337609‑3337788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑