BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,340,190	A→G	T93A (ACT→GCT)	yrbI →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,340,190	0	A	G	99.8%	1480.8 / ‑5.4	457	T93A (ACT→GCT)	yrbI	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (207/248); total (208/249)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.70e-01

Only 100 of 489 total aligned reads displayed.

CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340100‑3340279

cGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgct                                                                                         >  2:4523394/1‑93 (MQ=255)

 gTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctc                                                                                        >  2:8114877/1‑93 (MQ=255)

 gTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctc                                                                                        >  1:2848104/1‑93 (MQ=255)

  ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctca                                                                                       <  2:9348871/93‑1 (MQ=255)

  ttGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctca                                                                                       <  2:8284225/93‑1 (MQ=255)

   tgtgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGAtcgctcac                                                                                      <  1:4429128/93‑1 (MQ=255)

    gtgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATcgctcact                                                                                     <  1:3965227/93‑1 (MQ=255)

     tgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACtt                                                                                    <  1:5976366/93‑1 (MQ=255)

     tgCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACtt                                                                                    >  2:726810/1‑93 (MQ=255)

      gcgcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTg                                                                                   <  1:5310645/93‑1 (MQ=255)

      gcgcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTg                                                                                   >  2:5634267/1‑93 (MQ=255)

        gcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTa                                                                                 <  1:1912791/93‑1 (MQ=255)

        gcTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTa                                                                                 >  1:8873459/1‑93 (MQ=255)

          tCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATc                                                                               >  2:779143/1‑93 (MQ=255)

           cACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCa                                                                              <  1:7554709/93‑1 (MQ=255)

            cccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTTTATCAg                                                                             <  2:4914747/92‑1 (MQ=255)

            aCCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAg                                                                             <  2:1815717/93‑1 (MQ=255)

            aCCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAg                                                                             >  1:2316532/1‑93 (MQ=255)

             ccTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgg                                                                            >  2:2986391/1‑93 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  2:4302479/1‑93 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  1:577787/1‑93 (MQ=255)

              ctctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAggg                                                                           >  1:1282414/1‑93 (MQ=255)

               tctGATATTGAAGTCTTGCTCATTTCCTGGCGAATGGCTAAGCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          <  1:8692305/93‑1 (MQ=255)

               tctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          <  1:144612/93‑1 (MQ=255)

                ctGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGc                                                                         <  1:2174593/93‑1 (MQ=255)

                 tGATATTGAAGTCGCTATCATTACTGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTTGGGATCGCTCACTTGTATCAGGGGcg                                                                        >  2:9205345/1‑92 (MQ=255)

                   atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      <  2:1067874/93‑1 (MQ=255)

                   atatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGt                                                                      <  2:2891929/93‑1 (MQ=255)

                    tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGta                                                                     >  1:8162209/1‑92 (MQ=255)

                    tatTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTc                                                                     >  2:9714444/1‑93 (MQ=255)

                      ttGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaa                                                                   >  1:10035708/1‑93 (MQ=255)

                        gAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaac                                                                 >  1:9647560/1‑93 (MQ=255)

                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                >  1:673438/1‑93 (MQ=255)

                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                <  2:6128141/93‑1 (MQ=255)

                         aaGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTcaaaca                                                                >  1:9429411/1‑93 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             >  1:2502527/1‑93 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             >  1:7290339/1‑93 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:6459191/93‑1 (MQ=255)

                            tCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCaaacaaac                                                             <  2:8705843/93‑1 (MQ=255)

                             cGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACt                                                            <  2:8515227/93‑1 (MQ=255)

                                tATCATTGCCGGGCGAAAGGCTAATCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  2:3984635/93‑1 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:921767/93‑1 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         >  2:3739113/1‑93 (MQ=255)

                                tATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGAt                                                         <  1:5059967/93‑1 (MQ=255)

                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        >  1:5529328/1‑93 (MQ=255)

                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        <  2:5661318/93‑1 (MQ=255)

                                 aTCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATc                                                        >  2:258810/1‑93 (MQ=255)

                                  tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  1:7786293/1‑93 (MQ=255)

                                  tCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCg                                                       >  2:2215242/1‑93 (MQ=255)

                                    aTTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGcc                                                     <  2:4425539/93‑1 (MQ=255)

                                    aTTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGcc                                                     >  1:7111488/1‑93 (MQ=255)

                                     ttACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCt                                                    >  2:3518997/1‑93 (MQ=255)

                                     ttACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCt                                                    >  2:3306618/1‑93 (MQ=255)

                                       aCCGGGGGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCttt                                                  <  1:7442539/93‑1 (MQ=255)

                                       aCCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCttt                                                  <  1:3023083/93‑1 (MQ=255)

                                        ccGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTa                                                 <  2:9840401/93‑1 (MQ=255)

                                            gCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGa                                             <  2:6557419/93‑1 (MQ=255)

                                            gCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGa                                             <  2:7589744/93‑1 (MQ=255)

                                              gAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATc                                           >  2:893852/1‑93 (MQ=255)

                                               aaaGGCTAAACTGGTAGAAGGTCGTTGTTCCACATTGGGGATCGCTCACTTGTAGCAGGGGCAGTAAAACAAACTGATCGCCTTTAGCGATct                                          <  2:6226859/93‑1 (MQ=255)

                                               aaaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATct                                          >  1:8998058/1‑93 (MQ=255)

                                                aaGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctg                                         >  1:4279338/1‑93 (MQ=255)

                                                 aGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgc                                        <  2:9489786/93‑1 (MQ=255)

                                                  gtcTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:7111268/91‑1 (MQ=255)

                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:2381322/93‑1 (MQ=255)

                                                  ggCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgct                                       <  2:747048/93‑1 (MQ=255)

                                                   gCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATctgctg                                      <  2:3354229/93‑1 (MQ=255)

                                                     tAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGa                                    >  2:1219080/1‑93 (MQ=255)

                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAgggg                                                                          >  2:6909424/1‑54 (MQ=255)

                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   >  1:1573922/1‑93 (MQ=255)

                                                      aaaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaa                                   <  2:1961062/93‑1 (MQ=255)

                                                       aaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaa                                  >  1:4995971/1‑93 (MQ=255)

                                                       aaCTGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGaaa                                  <  1:9080090/93‑1 (MQ=255)

                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               >  2:3094717/1‑93 (MQ=255)

                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               <  1:8484043/93‑1 (MQ=255)

                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               >  2:505226/1‑93 (MQ=255)

                                                          tGGTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAAc                               <  1:3172683/93‑1 (MQ=255)

                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              <  2:413886/93‑1 (MQ=255)

                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              >  1:8031913/1‑93 (MQ=255)

                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              >  1:3809402/1‑93 (MQ=255)

                                                           ggTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACt                              >  1:6044771/1‑93 (MQ=255)

                                                            gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             <  2:6029970/93‑1 (MQ=255)

                                                            gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             >  1:7657545/1‑93 (MQ=255)

                                                            gTAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTg                             <  2:1658338/93‑1 (MQ=255)

                                                             tAGAAGATCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTgg                            <  1:3427605/93‑1 (MQ=255)

                                                              agaagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGc                           <  2:135828/93‑1 (MQ=255)

                                                                aagaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGa                         <  2:8330395/93‑1 (MQ=255)

                                                                 agaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAt                        <  1:6471021/93‑1 (MQ=255)

                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       <  1:354972/93‑1 (MQ=255)

                                                                  gaTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGAtt                       <  1:4810515/93‑1 (MQ=255)

                                                                   aTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTg                      >  2:5876555/1‑93 (MQ=255)

                                                                   aTCGTTGTGCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTg                      >  1:6837393/1‑93 (MQ=255)

                                                                          tgCCACATTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGa               <  1:2587997/93‑1 (MQ=255)

                                                                             cacaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGaaaa            <  2:2078978/93‑1 (MQ=255)

                                                                              acaTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAt           >  2:4793599/1‑93 (MQ=255)

                                                                               caTTGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtg          <  1:2756764/93‑1 (MQ=255)

                                                                                 ttGGGGATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAAtgtg        >  1:63376/1‑93 (MQ=255)

                                                                                   ggggATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGc      >  1:6181277/1‑93 (MQ=255)

                                                                                      gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  1:325216/93‑1 (MQ=255)

                                                                                      gATCGCTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTa   <  1:4525929/93‑1 (MQ=255)

CGTTGTGCGCTCACCTCTGATATTGAAGTCGCTATCATTACCGGGCGAAAGGCTAAACTGGTAGAAGATCGTTGTGCCACATTGGGGATCACTCACTTGTATCAGGGGCAGTCAAACAAACTGATCGCCTTTAGCGATCTGCTGGAAAAACTGGCGATTGCCCCGGAAAATGTGGCTTAT  >  NC_000913/3340100‑3340279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`