BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,358,838	G→A	A10T (GCT→ACT)	gltF →	GltF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,358,838	0	G	A	99.2%	874.5 / ‑5.5	240	A10T (GCT→ACT)	gltF	GltF
Reads supporting (aligned to +/- strand): ref base G (2/0); new base A (212/25); total (215/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 251 total aligned reads displayed.

GATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTA  >  NC_000913/3358748‑3358926

    tatataCTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTa                                                                                    >  2:9065472/1‑93 (MQ=255)

    tatataCTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTa                                                                                    <  2:5845291/93‑1 (MQ=255)

       ataCTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTAttt                                                                                 >  1:9754431/1‑93 (MQ=255)

          cTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTtgtg                                                                              >  2:4346217/1‑93 (MQ=255)

          cTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTtgtg                                                                              >  1:176870/1‑93 (MQ=255)

            gCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTgca                                                                            >  2:8839914/1‑93 (MQ=255)

            gCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTgca                                                                            >  2:1435436/1‑93 (MQ=255)

              ttCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTgcagc                                                                          >  2:2825400/1‑93 (MQ=255)

               tCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTgcagca                                                                         >  1:2746155/1‑93 (MQ=255)

                 aGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACt                                                                       >  2:2105699/1‑93 (MQ=255)

                    tttttATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTc                                                                    >  1:7000628/1‑93 (MQ=255)

                     ttttattttaAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCt                                                                   <  2:9802496/93‑1 (MQ=255)

                        tattttaAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGtt                                                                <  2:1236187/93‑1 (MQ=255)

                        tattttaAAAAATAAATTTGTTATTAAGGATATGGTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGtt                                                                >  1:1070778/1‑93 (MQ=255)

                          ttttaAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgc                                                              >  2:3993148/1‑93 (MQ=255)

                          ttttaAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgc                                                              >  2:7327591/1‑93 (MQ=255)

                            ttaAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgcag                                                            >  1:6605173/1‑93 (MQ=255)

                             taAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgcagc                                                           >  2:6928369/1‑93 (MQ=255)

                             taAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgcagc                                                           >  2:7433616/1‑93 (MQ=255)

                             taAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTgcagc                                                           >  2:8211381/1‑93 (MQ=255)

                               aaaaaTAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCAt                                                         >  1:1171560/1‑93 (MQ=255)

                               aaaaaTAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCAt                                                         >  2:7256757/1‑93 (MQ=255)

                                aaaaTAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCAtt                                                        >  1:4639321/1‑93 (MQ=255)

                                aaaaTAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCAtt                                                        >  1:686217/1‑93 (MQ=255)

                                aaaaTAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCAtt                                                        >  2:7000298/1‑93 (MQ=255)

                                 aaataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTc                                                       >  1:8101861/1‑93 (MQ=255)

                                   ataaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAg                                                     >  2:4054076/1‑93 (MQ=255)

                                     aaatTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTg                                                   >  1:2687174/1‑93 (MQ=255)

                                       atTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGcc                                                 >  2:3592129/1‑93 (MQ=255)

                                       atTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGcc                                                 >  2:2214883/1‑93 (MQ=255)

                                       atTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGcc                                                 >  2:8405890/1‑93 (MQ=255)

                                         ttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCAt                                               >  2:3356200/1‑93 (MQ=255)

                                         ttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTATGTTGCAGCATTCAGTGCCAt                                               >  1:6381526/1‑93 (MQ=255)

                                         ttGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTGTCTGTTGCAGCATTCAGTGCCAt                                               >  2:5609889/1‑93 (MQ=255)

                                          tGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATg                                              >  1:7315222/1‑93 (MQ=255)

                                          tGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATg                                              >  2:5066897/1‑93 (MQ=255)

                                           gTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATgg                                             >  2:1067753/1‑93 (MQ=255)

                                           gTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATgg                                             >  1:7843668/1‑93 (MQ=255)

                                             tattaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCa                                           >  1:6071691/1‑93 (MQ=255)

                                             tattaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCa                                           >  2:9325779/1‑93 (MQ=255)

                                              attaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCaa                                          >  1:9658754/1‑93 (MQ=255)

                                              attaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCaa                                          >  1:2092858/1‑93 (MQ=255)

                                              attaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCaa                                          >  2:8544872/1‑93 (MQ=255)

                                               ttaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAAc                                         >  2:9736893/1‑93 (MQ=255)

                                               ttaAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAAc                                         >  2:3106634/1‑93 (MQ=255)

                                                  aGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGa                                      >  2:1126561/1‑93 (MQ=255)

                                                  aGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGa                                      >  2:7442187/1‑93 (MQ=255)

                                                  aGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGa                                      >  1:2513485/1‑93 (MQ=255)

                                                  aGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGa                                      >  2:3887530/1‑93 (MQ=255)

                                                   ggATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAt                                     >  2:7457226/1‑93 (MQ=255)

                                                    gATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAtt                                    >  1:2150909/1‑93 (MQ=255)

                                                    gATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAtt                                    >  1:1103023/1‑93 (MQ=255)

                                                    gATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGAtt                                    >  2:3277468/1‑93 (MQ=255)

                                                     atgtGTTCATATGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTc                                   >  2:5720457/4‑93 (MQ=255)

                                                     atatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTc                                   <  2:5855784/93‑1 (MQ=255)

                                                      tttGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCTATGGCAACAGATTCa                                  >  1:8942837/3‑93 (MQ=255)

                                                      tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                  >  2:2934680/1‑93 (MQ=255)

                                                      tatGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCa                                  >  1:8014620/1‑93 (MQ=255)

                                                       atGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCaa                                 <  2:596013/93‑1 (MQ=255)

                                                       atGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCaa                                 >  2:7380849/1‑93 (MQ=255)

                                                       atGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCaa                                 <  1:5608050/93‑1 (MQ=255)

                                                        tGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAAc                                >  1:8102167/1‑93 (MQ=255)

                                                        tGTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAAc                                >  2:6512705/1‑93 (MQ=255)

                                                         gTTCATATGTTTTTCAAAAAGAACCTCACAACATCTGCTATCTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACt                               >  1:4973586/1‑93 (MQ=255)

                                                         gTTCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACt                               >  1:469837/1‑93 (MQ=255)

                                                          ttCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTg                              >  2:943848/1‑93 (MQ=255)

                                                          ttCATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTg                              >  2:9522801/1‑93 (MQ=255)

                                                            cATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGAt                            >  1:8630284/1‑93 (MQ=255)

                                                            cATATGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGAt                            <  1:1435436/93‑1 (MQ=255)

                                                             atatGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATa                           >  1:3182920/1‑93 (MQ=255)

                                                             atatGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATa                           >  2:7957966/1‑93 (MQ=255)

                                                               atGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACa                         >  2:4667226/1‑93 (MQ=255)

                                                                tGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCaa                                 <  1:7626683/84‑1 (MQ=255)

                                                                tGTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAg                        >  2:825251/1‑93 (MQ=255)

                                                                 gTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGa                       >  2:5682389/1‑93 (MQ=255)

                                                                 gTTTTTCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGa                       >  1:9171373/1‑93 (MQ=255)

                                                                   ttttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAAt                     >  1:3861728/1‑93 (MQ=255)

                                                                   ttttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAAt                     >  1:8814668/1‑93 (MQ=255)

                                                                    tttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAAtt                    >  1:4814148/1‑93 (MQ=255)

                                                                    tttCAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAAtt                    >  1:7535704/1‑93 (MQ=255)

                                                                       cAAAAAGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAAc                 >  1:1890900/1‑93 (MQ=255)

                                                                        aaaaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACt                >  2:9146516/1‑93 (MQ=255)

                                                                         aaaaGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTa               >  1:9968398/1‑93 (MQ=255)

                                                                            aGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatca            >  2:756963/1‑93 (MQ=255)

                                                                            aGAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatca            >  2:1874453/1‑93 (MQ=255)

                                                                             gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat           >  2:7841650/1‑93 (MQ=255)

                                                                             gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat           >  1:1348666/1‑93 (MQ=255)

                                                                             gAACCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcat           >  2:2405018/1‑93 (MQ=255)

                                                                              aaCCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcatc          >  2:7550937/1‑93 (MQ=255)

                                                                              aaCCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTatcatc          >  1:5683903/1‑93 (MQ=255)

                                                                               aCCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCg         >  2:8309030/1‑93 (MQ=255)

                                                                               aCCTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCg         >  1:994511/1‑93 (MQ=255)

                                                                                ccTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCgg        >  1:7109176/1‑93 (MQ=255)

                                                                                ccTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCgg        <  1:138148/93‑1 (MQ=255)

                                                                                ccTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCgg        >  2:4252580/1‑93 (MQ=255)

                                                                                 cTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGc       >  1:5901507/1‑93 (MQ=255)

                                                                                 cTCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGc       >  2:1973241/1‑93 (MQ=255)

                                                                                  tCACAACAACTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCg      >  2:6822238/1‑93 (MQ=255)

                                                                                     caacaaCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGt   <  2:3229040/93‑1 (MQ=255)

                                                                                     caacaaCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGt   <  1:2825400/93‑1 (MQ=255)

GATATATATACTGCTTCAGCTTTTTATTTTAAAAAATAAATTTGTTATTAAGGATATGTTCATATGTTTTTCAAAAAGAACCTCACAACAGCTGCTATTTGTGCAGCACTTTCTGTTGCAGCATTCAGTGCCATGGCAACAGATTCAACTGATACAGAATTAACTATCATCGGCGAGTA  >  NC_000913/3358748‑3358926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`