BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,054	A→G	H198R (CAC→CGC)	yhcG →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,054	0	A	G	99.8%	1305.6 / NA	410	H198R (CAC→CGC)	yhcG	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (197/212); total (198/212)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 454 total aligned reads displayed.

GGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACACGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAG  >  NC_000913/3365964‑3366143

ggTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAAcgcg                                                                                         >  2:1237324/1‑93 (MQ=255)

 gTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACgcgc                                                                                        <  1:4316492/93‑1 (MQ=255)

  tCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCt                                                                                       >  1:674204/1‑93 (MQ=255)

   cTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTc                                                                                      >  1:5186053/1‑93 (MQ=255)

   cTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTc                                                                                      <  1:8125441/93‑1 (MQ=255)

   cTGTNCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGNGGACACTACTGTNACNTGACAAATCAGCCATGCTGCAACAACGCGCTc                                                                                      >  1:4755039/1‑93 (MQ=255)

    tGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTcc                                                                                     >  1:124227/1‑93 (MQ=255)

     gTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCt                                                                                    >  1:1115785/1‑93 (MQ=255)

     gTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCt                                                                                    >  1:1318489/1‑93 (MQ=255)

          ccAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCga                                                                               <  2:8443820/93‑1 (MQ=255)

          ccAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGAAAAAAAGAGCTCCTGCCga                                                                               >  1:2011811/1‑93 (MQ=255)

          acAGCAAGAGCGGCAAATTGCGAACCAGTTTCATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCga                                                                               <  2:2161603/92‑1 (MQ=255)

           cAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgag                                                                              >  2:1031993/1‑93 (MQ=255)

           cAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgag                                                                              >  1:3612867/1‑93 (MQ=255)

           cAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgag                                                                              >  2:1775308/1‑93 (MQ=255)

            aGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCgaga                                                                             >  1:713094/1‑93 (MQ=255)

             gCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGAc                                                                            >  1:3312592/1‑93 (MQ=255)

             gCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGAc                                                                            <  2:1989907/93‑1 (MQ=255)

             gCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCCGCAACAACGCGCTCCTGCCGAGAc                                                                            >  1:5342534/1‑93 (MQ=255)

               tAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGc                                                                          <  1:891866/93‑1 (MQ=255)

               tAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGc                                                                          >  2:9704995/1‑93 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         >  2:7018417/1‑93 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         <  1:1621376/93‑1 (MQ=255)

                agagCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCa                                                                         >  1:7204861/1‑93 (MQ=255)

                  agCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCata                                                                       <  1:230423/93‑1 (MQ=255)

                   gCGGCAAATTGCGACCCAGTTTAATGAGCGAACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCatat                                                                      <  2:8809317/93‑1 (MQ=255)

                    cGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAtt                                                                     >  1:2499931/1‑93 (MQ=255)

                    cGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAtt                                                                     <  2:7866281/93‑1 (MQ=255)

                     ggCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttc                                                                    >  2:9911071/1‑93 (MQ=255)

                      gCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATAttct                                                                   <  2:2938640/93‑1 (MQ=255)

                        aaaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATTCTGCAACAACGTGCTCCTGCCGAGACGCATAttgttc                                                                 >  2:5301035/1‑93 (MQ=255)

                         aaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTcc                                                                <  1:6354800/93‑1 (MQ=255)

                         aaTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTcc                                                                >  1:687719/1‑93 (MQ=255)

                          aTTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCg                                                               >  1:992151/1‑93 (MQ=255)

                               gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          <  1:3731525/93‑1 (MQ=255)

                               gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          <  2:3288646/93‑1 (MQ=255)

                               gACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACa                                                          <  2:50705/93‑1 (MQ=255)

                                aCCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAg                                                         >  1:1737440/1‑93 (MQ=255)

                                aCCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAg                                                         >  1:6516720/1‑93 (MQ=255)

                                aCCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAg                                                         >  1:4624099/1‑93 (MQ=255)

                                 cccAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAgg                                                        <  2:6940106/93‑1 (MQ=255)

                                  ccAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGc                                                       >  1:8676728/1‑93 (MQ=255)

                                   cAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCg                                                      >  2:1621494/1‑93 (MQ=255)

                                    aGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGa                                                     <  1:8502298/93‑1 (MQ=255)

                                     gTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGAt                                                    <  2:8567236/93‑1 (MQ=255)

                                      ttttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATa                                                   <  2:3096254/93‑1 (MQ=255)

                                       tttATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATAc                                                  <  1:5649991/93‑1 (MQ=255)

                                         tATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATAcgc                                                <  2:8253797/93‑1 (MQ=255)

                                          aTGAGCGCACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATAcgcg                                               <  2:2636085/93‑1 (MQ=255)

                                           tGAGCGGACACTCCTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGa                                              <  1:8462932/93‑1 (MQ=255)

                                             aGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATc                                            <  1:1559916/93‑1 (MQ=255)

                                               cGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATccc                                          >  2:5720444/1‑93 (MQ=255)

                                                ggACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCt                                         >  1:3940040/1‑93 (MQ=255)

                                                ggACACTACTGTCACATGACAAACCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCt                                         <  1:5772086/93‑1 (MQ=255)

                                                 gACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCCTATTCTTCCGCAACAGGCGATACGCGATCCCtt                                        >  1:9690230/1‑93 (MQ=255)

                                                 gACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCtt                                        <  2:4259372/93‑1 (MQ=255)

                                                  acacTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCttt                                       <  1:9607502/93‑1 (MQ=255)

                                                  acacTACTGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCttt                                       <  1:311434/93‑1 (MQ=255)

                                                     ctactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTtgtg                                    >  2:6134076/1‑93 (MQ=255)

                                                     ctactGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGAGATCCGTTtgtg                                    >  1:7941478/1‑93 (MQ=255)

                                                       actGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCt                                  >  1:8228282/1‑93 (MQ=255)

                                                       actGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCt                                  >  1:2988425/1‑93 (MQ=255)

                                                        ctGTCACATGACAAATCAGCCATGCTGCAACACCGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTc                                 <  1:9200165/93‑1 (MQ=255)

                                                        ctGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTc                                 <  1:7502992/93‑1 (MQ=255)

                                                         tGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGcgcg                                >  2:7271647/1‑93 (MQ=255)

                                                         tGTCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCg                                >  1:8793508/1‑93 (MQ=255)

                                                           tCACATGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGaa                              >  1:260320/1‑93 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  2:4028241/1‑93 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  2:7371549/1‑93 (MQ=255)

                                                             acaTGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAAtt                            >  2:6652795/1‑93 (MQ=255)

                                                                tGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCt                         <  1:5398395/93‑1 (MQ=255)

                                                                tGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCt                         >  2:3903240/1‑93 (MQ=255)

                                                                tGACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCt                         <  2:7758817/93‑1 (MQ=255)

                                                                 gACAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTg                        <  2:9402180/93‑1 (MQ=255)

                                                                   cAAATCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGa                      <  2:3346083/93‑1 (MQ=255)

                                                                    aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     <  2:2214844/93‑1 (MQ=255)

                                                                    aaaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGaa                     >  1:7735798/1‑93 (MQ=255)

                                                                     aaTCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAAt                    >  1:4462329/1‑93 (MQ=255)

                                                                       tCAGCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGACTTTCTGGACTTg                  >  1:7063488/1‑93 (MQ=255)

                                                                         aGCCATGCTGCAACACCGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaa                <  2:1925091/93‑1 (MQ=255)

                                                                          gCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               <  2:9501683/93‑1 (MQ=255)

                                                                          gCCATGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGaaa               <  2:3654455/93‑1 (MQ=255)

                                                                             aTGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGAt            <  2:6366142/93‑1 (MQ=255)

                                                                             aTGCTGCAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGAt            <  2:7543158/93‑1 (MQ=255)

                                                                              tgctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATg           >  1:10027778/1‑93 (MQ=255)

                                                                               gctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGa          <  2:9186165/93‑1 (MQ=255)

                                                                               gctgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGa          <  1:6014506/93‑1 (MQ=255)

                                                                                 tgcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAat        >  2:5894767/1‑93 (MQ=255)

                                                                                  tcaacaaCGCCCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAata       <  2:3548481/92‑1 (MQ=255)

                                                                                  gcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAata       >  2:4501169/1‑93 (MQ=255)

                                                                                  gcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAata       >  1:7543815/1‑93 (MQ=255)

                                                                                  gcAACAACGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAata       <  1:4951796/93‑1 (MQ=255)

                                                                                   caacaaCGCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAatat      <  2:2474270/93‑1 (MQ=255)

                                                                                     acaacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTc    >  2:6850527/1‑93 (MQ=255)

                                                                                     acaacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTc    >  2:4744712/1‑93 (MQ=255)

                                                                                     acaacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTc    <  1:2872600/93‑1 (MQ=255)

                                                                                     acaacgCACTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTc    >  2:1339654/1‑93 (MQ=255)

                                                                                       aacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAg  >  1:3828260/1‑93 (MQ=255)

                                                                                       aacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTAAg  >  1:4153001/1‑93 (MQ=255)

                                                                                       aacgCGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGAGCTCGAATTTCTGGAATTGAAAGATGAATATTCAg  >  2:281739/1‑93 (MQ=255)

GGTCTGTTCGCCAGCTAGAGCGGCAAATTGCGACCCAGTTTTATGAGCGGACACTACTGTCACATGACAAATCAGCCATGCTGCAACAACACGCTCCTGCCGAGACGCATATTCTTCCGCAACAGGCGATACGCGATCCCTTTGTGCTCGAATTTCTGGAATTGAAAGATGAATATTCAG  >  NC_000913/3365964‑3366143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`