Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,374,589 A→G L156P (CTG→CCG)  sspA ← stringent starvation protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,374,5890AG99.7% 923.0 / ‑5.0 293L156P (CTG→CCG) sspAstringent starvation protein A
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (148/144);  total (149/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.78e-01

Only 100 of 311 total aligned reads displayed.

TAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCA  >  NC_000913/3374499‑3374678
                                                                                          |                                                                                         
taGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCggg                                                                                         >  2:7863920/1‑93 (MQ=255)
 aGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGc                                                                                        <  1:1581087/93‑1 (MQ=255)
 aGCCTTGCAGCCCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGTGGCAGACGCCACAGCACCGGAGCAAGAAAGCAATCGACCGGGc                                                                                        >  2:8961781/1‑93 (MQ=255)
  gCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCt                                                                                       >  2:8351867/1‑93 (MQ=255)
  gCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCt                                                                                       >  2:27013/1‑93 (MQ=255)
   ccTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGTCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTg                                                                                      <  1:4599632/93‑1 (MQ=255)
   ccTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTg                                                                                      <  1:8190559/93‑1 (MQ=255)
     tttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCTGCGGAGCAAGATAGCAATCGAACGGGCTGaa                                                                                    >  1:1673052/1‑93 (MQ=255)
     tttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGaa                                                                                    <  1:9586477/93‑1 (MQ=255)
      ttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAAc                                                                                   >  2:2637555/1‑93 (MQ=255)
      ttCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAAc                                                                                   <  2:741429/93‑1 (MQ=255)
       tCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACt                                                                                  <  1:10045280/93‑1 (MQ=255)
        cAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTc                                                                                 >  1:2897076/1‑93 (MQ=255)
         aGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCa                                                                                >  1:4734957/1‑93 (MQ=255)
         aGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCa                                                                                >  2:5739347/1‑93 (MQ=255)
         aGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCa                                                                                >  2:6434911/1‑93 (MQ=255)
          gCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCAt                                                                               >  1:8036337/1‑93 (MQ=255)
            tctTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCCTCg                                                                             >  1:6303255/1‑93 (MQ=255)
            tctTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCg                                                                             >  2:6210623/1‑93 (MQ=255)
             ctTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGc                                                                            >  2:2508780/1‑93 (MQ=255)
                tCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCa                                                                         <  1:6458634/93‑1 (MQ=255)
                 cGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGAAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAg                                                                        >  1:3445989/1‑93 (MQ=255)
                    aCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGaa                                                                     >  1:3853620/1‑93 (MQ=255)
                    aCCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGaa                                                                     <  2:1947095/93‑1 (MQ=255)
                     cccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAg                                                                    >  1:4040050/1‑93 (MQ=255)
                     cccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAg                                                                    >  2:1197949/1‑93 (MQ=255)
                     cccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAg                                                                    >  2:4306893/1‑93 (MQ=255)
                      ccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCGTCGCTCAGGAAGt                                                                   >  2:8472237/1‑93 (MQ=255)
                      ccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAgc                                                                   >  1:1463274/1‑92 (MQ=255)
                      ccGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGt                                                                   <  1:6616273/93‑1 (MQ=255)
                       cGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGtc                                                                  >  2:4433060/1‑92 (MQ=255)
                       cGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTa                                                                  <  2:3932406/93‑1 (MQ=255)
                        gggCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTAc                                                                 >  2:4072143/1‑93 (MQ=255)
                        gggCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTAc                                                                 <  1:9413846/93‑1 (MQ=255)
                         ggCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACg                                                                <  1:3193224/93‑1 (MQ=255)
                          gCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACgg                                                               <  2:3866055/93‑1 (MQ=255)
                          gCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACgg                                                               <  2:4814131/93‑1 (MQ=255)
                           ccGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGc                                                              <  1:7320062/93‑1 (MQ=255)
                              cTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTc                                                           <  2:7743911/93‑1 (MQ=255)
                               tGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCt                                                          >  1:2822322/1‑93 (MQ=255)
                                 aaCTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGa                                                        <  1:9834950/93‑1 (MQ=255)
                                   cTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGAcc                                                      >  1:5970293/1‑93 (MQ=255)
                                    tCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCg                                                     >  1:5388233/1‑93 (MQ=255)
                                    tCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCg                                                     >  1:2790914/1‑93 (MQ=255)
                                      gATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGaa                                                   >  1:7795380/1‑93 (MQ=255)
                                      gATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGaa                                                   <  1:5507573/93‑1 (MQ=255)
                                      gATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGaa                                                   <  2:7478950/93‑1 (MQ=255)
                                        tGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGa                                                 >  1:5779763/1‑93 (MQ=255)
                                        tGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGa                                                 <  2:5272921/93‑1 (MQ=255)
                                         gCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGAc                                                <  1:1911657/93‑1 (MQ=255)
                                          cccAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGAcc                                               <  2:7041495/93‑1 (MQ=255)
                                           ccAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTCCGGCTTCTGACAGAAGACCg                                              >  1:1163282/1‑93 (MQ=255)
                                           ccAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCg                                              >  2:7139021/1‑93 (MQ=255)
                                            cAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAACACCgg                                             >  1:3611886/1‑93 (MQ=255)
                                              gTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcg                                           >  2:1046229/1‑93 (MQ=255)
                                               ttGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcgc                                          >  2:5514828/1‑93 (MQ=255)
                                               ttGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGgcgc                                          >  2:3690499/1‑93 (MQ=255)
                                                  cGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAAt                                       <  1:2055196/93‑1 (MQ=255)
                                                   ggCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATc                                      >  2:510813/1‑93 (MQ=255)
                                                     cAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGc                                    <  1:8366736/93‑1 (MQ=255)
                                                      aGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGcc                                   >  1:2867495/1‑93 (MQ=255)
                                                      aGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGcc                                   <  2:2180066/93‑1 (MQ=255)
                                                       gACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCca                                  >  1:5203021/1‑93 (MQ=255)
                                                       gACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCca                                  >  2:2827435/1‑93 (MQ=255)
                                                       gACGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCca                                  >  2:1628569/1‑93 (MQ=255)
                                                        aCGCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcag                                 <  1:6883011/93‑1 (MQ=255)
                                                          gCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcagca                               >  1:920192/1‑93 (MQ=255)
                                                          gCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcagca                               >  2:6940031/1‑93 (MQ=255)
                                                          gCCACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcagca                               <  1:8790150/93‑1 (MQ=255)
                                                           ccACAGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCcagcag                              <  2:2346764/93‑1 (MQ=255)
                                                            cacaGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGt                             <  2:1474451/93‑1 (MQ=255)
                                                             acaGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGtt                            >  1:6023088/1‑93 (MQ=255)
                                                             acaGCAGCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGtt                            >  1:2195030/1‑93 (MQ=255)
                                                              cagcagCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttc                           >  2:2749924/1‑93 (MQ=255)
                                                              cagcagCGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttc                           <  2:794582/93‑1 (MQ=255)
                                                               agcagcGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttct                          <  2:3356430/93‑1 (MQ=255)
                                                                gcagcGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCCGCGCAATCGCCAGCAGttctt                         >  2:3438745/1‑93 (MQ=255)
                                                                 cagcGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGttcttc                        <  2:7005700/93‑1 (MQ=255)
                                                                  agcGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcg                       <  2:9687671/93‑1 (MQ=255)
                                                                   gcGGAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTcgc                      >  1:10007922/1‑93 (MQ=255)
                                                                     ggAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCgcgc                    <  2:6522491/93‑1 (MQ=255)
                                                                      gAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCa                   <  2:9299598/93‑1 (MQ=255)
                                                                      gAGCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCa                   <  2:6722153/93‑1 (MQ=255)
                                                                       aGCAAGAAAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAg                  >  2:1397359/1‑93 (MQ=255)
                                                                        gCAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGt                 <  2:6618979/93‑1 (MQ=255)
                                                                         cAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGtt                <  1:8536403/93‑1 (MQ=255)
                                                                         cAAGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGtt                >  1:5549045/1‑93 (MQ=255)
                                                                           aGATAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGc              <  1:8099399/93‑1 (MQ=255)
                                                                            aaTAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCt             <  2:8573672/92‑1 (MQ=255)
                                                                             aTAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGACAGCAGTTCTTCGCGCAGTTGCtt            >  1:4248711/1‑93 (MQ=255)
                                                                              tAGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTa           <  1:3010320/93‑1 (MQ=255)
                                                                               aGCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTAc          <  1:6374743/93‑1 (MQ=255)
                                                                                gCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACg         <  1:588212/93‑1 (MQ=255)
                                                                                gCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACg         <  2:4515882/93‑1 (MQ=255)
                                                                                gCAATCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACg         <  2:4932821/93‑1 (MQ=255)
                                                                                  aaTCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTg       <  1:7425186/93‑1 (MQ=255)
                                                                                  aaTCGACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTg       <  1:1824440/93‑1 (MQ=255)
                                                                                      gACCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGc   <  1:5107361/93‑1 (MQ=255)
                                                                                       aCCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCa  >  1:7556943/1‑93 (MQ=255)
                                                                                       aCCGGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCa  <  2:9164581/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAGCCTTTCAGCTCTTTCGCACCCGGGCCGCTGAACTCGATGCCCAGTTGCGGCAGACGCCACAGCAGCGGAGCAAGATAGCAATCGACCAGGCTGAACTCATCGCTCAGGAAGTACGGCTTCTGACCGAAGACCGGCGCAATCGCCAGCAGTTCTTCGCGCAGTTGCTTACGTGCGGCA  >  NC_000913/3374499‑3374678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑