BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,407,232	G→A	A176T (GCG→ACG)	prmA →	PrmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,407,232	0	G	A	99.2%	1344.4 / ‑6.4	376	A176T (GCG→ACG)	prmA	PrmA
Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (205/168); total (205/171)
Fisher's exact test for biased strand distribution p-value = 9.32e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

TCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGC  >  NC_000913/3407142‑3407321

tctctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCgcgacg                                                                                         >  2:3533065/1‑93 (MQ=255)

tctctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCgcgacg                                                                                         >  2:9148710/1‑93 (MQ=255)

  tctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCt                                                                                       <  2:8406405/93‑1 (MQ=255)

  tctGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCt                                                                                       >  1:7650384/1‑93 (MQ=255)

    tgtgCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGa                                                                                     <  1:7960037/93‑1 (MQ=255)

       gCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAAc                                                                                  <  1:9164559/93‑1 (MQ=255)

        ccTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACt                                                                                 >  1:5634231/1‑93 (MQ=255)

          tGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATGGACTTTTGCTGTGGGTCCGGCATTCTGGCGATCGCGACGCTGAAACTgg                                                                               >  1:314054/1‑93 (MQ=255)

          tGCAATGGCTCGACAGCCTCGATTTAACCCGTAAAACAGTAATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACtcg                                                                               >  1:7869764/1‑91 (MQ=255)

           gCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTggg                                                                              <  1:8777263/93‑1 (MQ=255)

            cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGt                                                                             <  2:8706154/93‑1 (MQ=255)

            cAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGt                                                                             >  2:5145830/1‑93 (MQ=255)

             aaTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTg                                                                            >  1:3368636/1‑93 (MQ=255)

              aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgc                                                                           >  2:6648839/1‑93 (MQ=255)

              aTGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGGgc                                                                           >  2:8108402/1‑93 (MQ=255)

               tGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTgca                                                                          <  1:3790868/93‑1 (MQ=255)

                   tCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGCCGCTGAAATTGGGTGCAGCaa                                                                      >  1:402330/1‑93 (MQ=255)

                    cGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaa                                                                     <  1:8028697/93‑1 (MQ=255)

                    cGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaa                                                                     <  2:4587055/93‑1 (MQ=255)

                    cGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaa                                                                     >  2:3753085/1‑93 (MQ=255)

                     gACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCaaaa                                                                    >  1:6475636/1‑93 (MQ=255)

                       cAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGc                                                                  <  1:5236111/93‑1 (MQ=255)

                       cAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGc                                                                  <  1:1859712/93‑1 (MQ=255)

                         gCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCa                                                                <  1:2153812/93‑1 (MQ=255)

                          ccTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCAt                                                               >  2:7921079/1‑93 (MQ=255)

                           cTCGATTTAACCGGTAAAATAGTCATCGCCTTTGGCTGTGGTTCTGGCATTATCGCGGTCGCGCCGCTGAAATTGTGGGCAGCAAACGCCAtg                                                              >  1:8637977/1‑92 (MQ=255)

                            tCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTg                                                             >  1:8295670/1‑93 (MQ=255)

                             cGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTgg                                                            <  1:1613981/93‑1 (MQ=255)

                               aTTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTa                                                          >  2:7046772/1‑93 (MQ=255)

                               aTTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTa                                                          <  1:7598038/93‑1 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         <  2:10005591/93‑1 (MQ=255)

                                tttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAt                                                         >  1:944240/1‑93 (MQ=255)

                                 ttAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTAtt                                                        <  2:9600893/93‑1 (MQ=255)

                                  tAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTg                                                       <  1:10015967/93‑1 (MQ=255)

                                   aaCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGa                                                      >  2:3621187/1‑93 (MQ=255)

                                    aCCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGat                                                     >  2:4897058/1‑93 (MQ=255)

                                     ccGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGata                                                    >  1:9919047/1‑93 (MQ=255)

                                      cGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGGCATTGGTATTGatat                                                   >  2:730873/1‑93 (MQ=255)

                                       ggTAAAACAGTCATCGACTTTGGCTGTTGTTACGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGagatg                                                  >  2:6868116/1‑92 (MQ=255)

                                       ggTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATc                                                  >  2:1155255/1‑93 (MQ=255)

                                       ggTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATc                                                  >  1:1133301/1‑93 (MQ=255)

                                        gTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCg                                                 >  1:4778721/1‑93 (MQ=255)

                                         tAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGa                                                <  1:7317685/93‑1 (MQ=255)

                                         tAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGa                                                >  2:3967923/1‑93 (MQ=255)

                                         tAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGa                                                <  2:2683644/93‑1 (MQ=255)

                                         tAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGa                                                <  2:1196460/93‑1 (MQ=255)

                                          aaaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGAt                                               >  2:5264568/1‑93 (MQ=255)

                                           aaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATc                                              >  2:9294113/1‑93 (MQ=255)

                                           aaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATc                                              >  2:7868050/1‑93 (MQ=255)

                                           aaaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTAGTATTGATATCGATc                                              >  1:7769493/1‑93 (MQ=255)

                                            aaCAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATcc                                             <  2:5200785/93‑1 (MQ=255)

                                              cAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGc                                           <  2:7426839/93‑1 (MQ=255)

                                               aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  1:2747245/93‑1 (MQ=255)

                                               aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          <  1:8220956/93‑1 (MQ=255)

                                               aGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCa                                          >  1:22360/1‑93 (MQ=255)

                                                 tCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAgg                                        >  2:1550305/1‑93 (MQ=255)

                                                 tCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAgg                                        >  1:3922632/1‑93 (MQ=255)

                                                    tCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCACAAGCCATTGGTATTGATATCGATCCGCAGGCGa                                     >  1:9671084/1‑93 (MQ=255)

                                                    tCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa                                     >  2:7619805/1‑93 (MQ=255)

                                                    ccGACTGTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAACCTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGa                                     <  1:4207515/92‑1 (MQ=255)

                                                      gattttGGCTGTGGTCCGGGCTTTCTGGCGATCGCGACGCGAAACCGGGGTGCAGAAAAAGCAATTGTTATTGATATCGACCCGCGGGCGttt                                   <  2:4831417/90‑1 (MQ=255)

                                                      gACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAACCTGGGTGCACAACACGCCATTGGTATTGATATCGCTCCGCAGGCGAtt                                   >  1:2587376/1‑93 (MQ=255)

                                                      gACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGAtt                                   >  2:6395248/1‑93 (MQ=255)

                                                       aCTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTc                                  <  1:199772/93‑1 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 >  1:6825704/1‑93 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  2:297687/93‑1 (MQ=255)

                                                        cTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCa                                 <  1:1540891/93‑1 (MQ=255)

                                                         tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                >  2:5939289/1‑93 (MQ=255)

                                                         tttGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAg                                >  2:2460432/1‑93 (MQ=255)

                                                           tGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                              <  1:6198442/93‑1 (MQ=255)

                                                           tGGCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGc                              <  2:3429020/93‑1 (MQ=255)

                                                             gCTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCa                            <  2:6222356/93‑1 (MQ=255)

                                                              cTGTGGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAg                           >  1:5074855/1‑93 (MQ=255)

                                                               tgtgGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGc                          <  2:1920292/93‑1 (MQ=255)

                                                                gtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGcc                         >  2:5409907/1‑93 (MQ=255)

                                                                gtgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGcc                         <  1:5904557/93‑1 (MQ=255)

                                                                 tgGTTCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCcg                        >  1:1250840/1‑93 (MQ=255)

                                                                    ttCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGa                     <  2:8305161/93‑1 (MQ=255)

                                                                    ttCCGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGa                     >  2:4707999/1‑93 (MQ=255)

                                                                      ccGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATa                   <  2:9417602/93‑1 (MQ=255)

                                                                       cGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa                  <  1:5742690/93‑1 (MQ=255)

                                                                       cGGCATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATaa                  <  1:3272603/93‑1 (MQ=255)

                                                                          cATTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGc               >  1:6507734/1‑93 (MQ=255)

                                                                           aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcc              >  1:5368049/1‑93 (MQ=255)

                                                                           aTTCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGcc              >  2:2876976/1‑93 (MQ=255)

                                                                            ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg             >  1:292971/1‑93 (MQ=255)

                                                                            ttCTGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCg             >  2:75156/1‑93 (MQ=255)

                                                                               tGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAAc          >  2:1665614/1‑93 (MQ=255)

                                                                               tGGCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAAc          >  2:4352885/1‑93 (MQ=255)

                                                                                ggCGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACg         <  2:6036555/93‑1 (MQ=255)

                                                                                  cGATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTa       <  2:5569930/93‑1 (MQ=255)

                                                                                   gATGGCGACGCTGAAACTGGGTTCAGCAAAAGCCATTGGTATTGAAATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa      <  2:3297779/93‑1 (MQ=255)

                                                                                   gATCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTaa      <  2:1426384/93‑1 (MQ=255)

                                                                                    aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     >  1:9942187/1‑93 (MQ=255)

                                                                                    aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     <  2:421364/93‑1 (MQ=255)

                                                                                    aTCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAAt     >  1:8908305/1‑93 (MQ=255)

                                                                                     tCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATg    >  1:6904893/1‑93 (MQ=255)

                                                                                     tCGCGACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATg    >  1:9306415/1‑93 (MQ=255)

                                                                                      cgcgACGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATgg   <  1:7178532/93‑1 (MQ=255)

                                                                                       gcgacgCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGc  >  1:4566984/1‑93 (MQ=255)

TCTCTGTGCCTGCAATGGCTCGACAGCCTCGATTTAACCGGTAAAACAGTCATCGACTTTGGCTGTGGTTCCGGCATTCTGGCGATCGCGGCGCTGAAACTGGGTGCAGCAAAAGCCATTGGTATTGATATCGATCCGCAGGCGATTCAGGCCAGCCGCGATAACGCCGAACGTAATGGC  >  NC_000913/3407142‑3407321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`