BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,427,261	T→C	V130A (GTC→GCC)	b3279 →	putative transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,427,261	0	T	C	99.5%	1284.4 / ‑5.8	401	V130A (GTC→GCC)	b3279	putative transferase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (196/201); total (199/202)
Fisher's exact test for biased strand distribution p-value = 2.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 417 total aligned reads displayed.

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

ttGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGccc                                                                                         <  1:1430102/93‑1 (MQ=255)

ttGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGccc                                                                                         >  1:9304334/1‑93 (MQ=255)

ttGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGccc                                                                                         <  1:7000946/93‑1 (MQ=255)

  ggCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCca                                                                                       >  2:9367516/1‑93 (MQ=255)

   gCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGACCcac                                                                                      >  2:830118/1‑93 (MQ=255)

    cAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCcaca                                                                                     >  2:8485109/1‑93 (MQ=255)

    cAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCcaca                                                                                     >  1:8520449/1‑93 (MQ=255)

     aaTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaa                                                                                    >  1:1080036/1‑93 (MQ=255)

      aTCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaa                                                                                   >  1:7796156/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaaa                                                                                  >  2:119764/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaaa                                                                                  >  2:6282678/1‑93 (MQ=255)

       tCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACaaaa                                                                                  >  2:5722635/1‑93 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 >  2:1050418/1‑93 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  1:7127265/93‑1 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 >  2:4241307/1‑93 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  1:8561235/93‑1 (MQ=255)

        cGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAAt                                                                                 <  1:3517764/93‑1 (MQ=255)

         gAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATa                                                                                <  1:8307455/93‑1 (MQ=255)

           gTTTTTGTTGGGATGTGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  2:3342285/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTTGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              >  1:9553720/1‑93 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              >  2:3955045/1‑93 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  1:4623162/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  1:5014012/93‑1 (MQ=255)

           gTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATaaa                                                                              <  2:4529371/93‑1 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             >  1:7645157/1‑93 (MQ=255)

            ttttGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAAc                                                                             >  2:8669770/1‑93 (MQ=255)

                  ttggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTgg                                                                       >  2:8052051/1‑93 (MQ=255)

                   tggGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGga                                                                      <  1:9719659/93‑1 (MQ=255)

                    tggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgag                                                                     <  1:8197638/92‑1 (MQ=255)

                     ggATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGgaga                                                                    >  1:274921/1‑93 (MQ=255)

                      gATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGagag                                                                   <  2:3493616/93‑1 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCGACAAAATAAACGGCTGGAGAGCg                                                                 >  2:5604931/1‑93 (MQ=255)

                        tGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCg                                                                 <  2:4771654/93‑1 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  1:9913831/93‑1 (MQ=255)

                          ggCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGa                                                               <  2:9807189/93‑1 (MQ=255)

                            cTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGata                                                             >  1:3868360/1‑93 (MQ=255)

                             tCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGatat                                                            <  1:5063247/93‑1 (MQ=255)

                               aaTTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCt                                                          >  2:186272/1‑93 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  1:6202724/1‑93 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        <  1:8909751/93‑1 (MQ=255)

                                 ttttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGt                                                        >  2:9497886/1‑93 (MQ=255)

                                  tttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTa                                                       <  1:5677730/93‑1 (MQ=255)

                                   ttACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAt                                                      <  2:5087096/93‑1 (MQ=255)

                                    tACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtc                                                     <  2:3012809/93‑1 (MQ=255)

                                     aCTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtct                                                    >  2:2875679/1‑93 (MQ=255)

                                      cTTGATGGCGCAATAGTAGAAGATGTTGTGATGATTTGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtctc                                                   >  1:5549442/1‑93 (MQ=255)

                                      cTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTAtctc                                                   <  1:7510757/93‑1 (MQ=255)

                                       ttGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCg                                                  <  2:3159436/93‑1 (MQ=255)

                                        tGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCgg                                                 >  1:6531831/1‑93 (MQ=255)

                                         gATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGt                                                <  2:5378184/93‑1 (MQ=255)

                                         gATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGt                                                <  2:2029397/93‑1 (MQ=255)

                                               gcAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGcccc                                          <  1:1218432/93‑1 (MQ=255)

                                                cAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCg                                         >  2:6857091/1‑93 (MQ=255)

                                                     gtagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCaaa                                    <  2:2681869/93‑1 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCcaaa                                   >  1:4246243/1‑92 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   >  1:5405282/1‑93 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   >  1:4876831/1‑93 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   >  1:9211677/1‑93 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   <  2:7064614/93‑1 (MQ=255)

                                                      tagAAGATGATGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAAc                                   >  2:2529218/1‑93 (MQ=255)

                                                         aagaTGATGTGATGATTGGTACGGGTAGTCTGGCCCGACAAAATACACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGa                                <  1:1835920/93‑1 (MQ=255)

                                                           gatgatGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATc                              <  2:6581841/93‑1 (MQ=255)

                                                           gatgatGTGATGATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATc                              >  2:1627946/1‑93 (MQ=255)

                                                               atgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGcc                          >  1:3179889/1‑93 (MQ=255)

                                                                tgtgatgATTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGccc                         <  1:734750/93‑1 (MQ=255)

                                                                  tgatgaTTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGt                       >  2:7251659/1‑93 (MQ=255)

                                                                  tgatgaTTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGt                       >  2:2919403/1‑93 (MQ=255)

                                                                   gatgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGtt                      >  2:1732150/1‑93 (MQ=255)

                                                                   gatgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGtt                      <  1:3732308/93‑1 (MQ=255)

                                                                    atgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCGCGGTa                     >  2:6800651/1‑93 (MQ=255)

                                                                    atgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTa                     <  2:9084717/93‑1 (MQ=255)

                                                                    atgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTa                     <  2:2301371/93‑1 (MQ=255)

                                                                    atgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTa                     <  1:3590464/93‑1 (MQ=255)

                                                                     tgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTaa                    >  1:2963683/1‑93 (MQ=255)

                                                                     tgatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTaa                    >  1:10029725/1‑93 (MQ=255)

                                                                      gatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAg                   >  1:5201300/1‑93 (MQ=255)

                                                                      gatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAg                   <  2:2171442/93‑1 (MQ=255)

                                                                      gatTGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATACGCCCGTTAAg                   >  1:5902678/1‑93 (MQ=255)

                                                                       atTGGTGCGGGTAGTCTGGACCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGt                  <  2:8948891/93‑1 (MQ=255)

                                                                        ttGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtg                 <  1:9089187/93‑1 (MQ=255)

                                                                         tGGTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGtga                <  2:9204085/93‑1 (MQ=255)

                                                                           gTGCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGggatg              >  1:3344213/1‑93 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  2:6909764/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  2:7437361/93‑1 (MQ=255)

                                                                             gCGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGaa            <  2:3759639/93‑1 (MQ=255)

                                                                              cGGGTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAag           >  1:2240060/1‑93 (MQ=255)

                                                                               gggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAaga          <  1:6013099/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         >  2:2742254/1‑93 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         <  1:2330219/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         <  1:1992539/93‑1 (MQ=255)

                                                                                ggTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAagag         >  2:8575845/1‑93 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        >  2:1318155/1‑93 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        >  1:2908422/1‑93 (MQ=255)

                                                                                 gTAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAgaga        <  2:7915735/93‑1 (MQ=255)

                                                                                  tAGTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGaa       <  2:4990684/93‑1 (MQ=255)

                                                                                    gTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAgg     >  2:5958447/1‑93 (MQ=255)

                                                                                    gTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAgg     <  2:135848/93‑1 (MQ=255)

                                                                                    gTCTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAgg     >  2:6867871/1‑93 (MQ=255)

                                                                                      cTGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCt   <  1:2612205/93‑1 (MQ=255)

                                                                                       tGGCCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTg  <  1:8370554/93‑1 (MQ=255)

TTGGCAATCGAGTTTTGGTTGGGATGGGCTCAATTTTACTTGATGGCGCAATAGTAGAAGATGATGTGATGATTGGTGCGGGTAGTCTGGTCCCACAAAATAAACGGCTGGAGAGCGGATATCTGTATCTCGGTAGCCCCGTCAAACAGATCCGCCCGTTAAGTGATGAAGAGAAGGCTG  >  NC_000913/3427171‑3427350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`