BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,454,538	T→C	N175N (AAT→AAC)	b3325 →	YheF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,454,538	0	T	C	99.8%	1465.0 / ‑5.2	444	N175N (AAT→AAC)	b3325	YheF
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (236/206); total (237/207)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 462 total aligned reads displayed.

CCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT  >  NC_000913/3454448‑3454627

ccAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATtaacaa                                                                                         <  1:605940/93‑1 (MQ=255)

 cAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACaaa                                                                                        <  1:5193817/93‑1 (MQ=255)

  aGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAc                                                                                       <  1:9048511/93‑1 (MQ=255)

  aGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAAc                                                                                       <  1:7074436/93‑1 (MQ=255)

     tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    >  1:5793575/1‑93 (MQ=255)

     tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    >  1:6516179/1‑93 (MQ=255)

     tgatgGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGa                                                                                    >  1:8489377/1‑93 (MQ=255)

           atgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAg                                                                              <  2:9775279/93‑1 (MQ=255)

            tgCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGt                                                                             <  2:1097047/93‑1 (MQ=255)

              ggggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCa                                                                           <  2:8897158/92‑1 (MQ=255)

               gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACACACTGATTGAAGTCAt                                                                          >  2:4409769/1‑93 (MQ=255)

               gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAt                                                                          >  2:8572037/1‑93 (MQ=255)

               gggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCAt                                                                          >  2:8415415/1‑93 (MQ=255)

                ggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATa                                                                         >  1:1767252/1‑93 (MQ=255)

                ggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATa                                                                         >  1:5366577/1‑93 (MQ=255)

                ggTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATa                                                                         >  2:6199224/1‑93 (MQ=255)

                 gTATCGTCGGTAATGTTGTGCATTATGAACCCTCCCACGTTCTTATTCTGCCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  1:1301232/93‑1 (MQ=255)

                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:8261009/93‑1 (MQ=255)

                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:8707814/93‑1 (MQ=255)

                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:9167528/93‑1 (MQ=255)

                 gTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaa                                                                        <  2:5406570/93‑1 (MQ=255)

                  gaGCGTCGGTAATATTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATaaa                                                                       <  1:8293848/92‑1 (MQ=255)

                   aGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAg                                                                      <  1:2354524/93‑1 (MQ=255)

                    gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     >  2:6521877/1‑93 (MQ=255)

                    gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     >  2:3109832/1‑93 (MQ=255)

                    gCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgc                                                                     <  1:2175900/93‑1 (MQ=255)

                      gTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAgcgc                                                                   <  2:5317625/93‑1 (MQ=255)

                       tCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACANANTGATTGAAGTCATAAAGcgcg                                                                  >  1:1866859/1‑93 (MQ=255)

                        cGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGt                                                                 >  2:3403674/1‑93 (MQ=255)

                         ggTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGtt                                                                <  2:7626986/93‑1 (MQ=255)

                          gTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTg                                                               <  2:2949724/93‑1 (MQ=255)

                           tAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGa                                                              <  1:8774453/93‑1 (MQ=255)

                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  1:1204837/1‑93 (MQ=255)

                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  2:8137197/1‑93 (MQ=255)

                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             >  2:6100563/1‑93 (MQ=255)

                            aaTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGAt                                                             <  2:878617/93‑1 (MQ=255)

                             aTGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATg                                                            >  1:10039753/1‑93 (MQ=255)

                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           <  2:4226650/93‑1 (MQ=255)

                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  2:355169/1‑93 (MQ=255)

                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:2472004/1‑93 (MQ=255)

                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:8093479/1‑93 (MQ=255)

                              tgttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGt                                                           >  1:4117976/1‑93 (MQ=255)

                               gttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTc                                                          >  1:8953812/1‑93 (MQ=255)

                                ttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCa                                                         >  2:9355125/1‑93 (MQ=255)

                                ttgtGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCa                                                         <  1:4813865/93‑1 (MQ=255)

                                   tgCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCg                                                      <  1:6432145/93‑1 (MQ=255)

                                       ttATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAGGTCATAAAGCGCGTTGATGTCATCGGcac                                                  <  1:6364113/93‑1 (MQ=255)

                                       ttATGAACCCTCCAACGTTCTTATTCTGACCGGTCATGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGcac                                                  >  1:1965225/1‑93 (MQ=255)

                                        tATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGcaca                                                 >  1:364253/1‑93 (MQ=255)

                                         aTGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACag                                                <  2:184289/93‑1 (MQ=255)

                                           gAACCCTCCAACGTTCTTTTTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACagag                                              >  1:5066519/1‑93 (MQ=255)

                                           gAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACagag                                              >  2:2262852/1‑93 (MQ=255)

                                            aaCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAgaga                                             <  2:211428/93‑1 (MQ=255)

                                             aCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGaa                                            <  1:5608555/93‑1 (MQ=255)

                                              cccTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAag                                           <  2:2546290/93‑1 (MQ=255)

                                               ccTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAaga                                          >  2:7398231/1‑92 (MQ=255)

                                                    aaCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaa                                     >  2:1542517/1‑93 (MQ=255)

                                                    aaCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaa                                     >  2:2181980/1‑93 (MQ=255)

                                                     aCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaaa                                    <  1:9408899/93‑1 (MQ=255)

                                                     aCGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCaaa                                    <  1:1628707/93‑1 (MQ=255)

                                                      cGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAat                                   <  2:7792796/93‑1 (MQ=255)

                                                       gTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatt                                  >  1:3167306/1‑93 (MQ=255)

                                                       gTTCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatt                                  >  1:7631721/1‑93 (MQ=255)

                                                        ttCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatta                                 <  1:1057633/93‑1 (MQ=255)

                                                        ttCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAatta                                 >  1:6760108/1‑93 (MQ=255)

                                                         tCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAattat                                <  2:6996984/93‑1 (MQ=255)

                                                         tCTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGCCACAGAGAAGCAGCACattat                                >  2:454904/1‑93 (MQ=255)

                                                          cTTATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAattatt                               >  1:9963236/1‑93 (MQ=255)

                                                            tATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCa                             <  1:7374399/93‑1 (MQ=255)

                                                            tATTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATCTCATCGGCACAGAGAAGCAGCAAATTATTCa                             >  2:1891832/1‑93 (MQ=255)

                                                             aTTCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCAt                            >  2:5708173/1‑93 (MQ=255)

                                                              ttCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATc                           <  2:5201237/93‑1 (MQ=255)

                                                              ttCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATc                           >  2:9594444/1‑93 (MQ=255)

                                                               tCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCt                          >  1:9727580/1‑93 (MQ=255)

                                                               tCTGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGGCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCt                          <  1:7884419/93‑1 (MQ=255)

                                                                 tGACGGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTgg                        <  2:3975519/93‑1 (MQ=255)

                                                                 tGACCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTgg                        >  2:7957824/1‑93 (MQ=255)

                                                                   aCCGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGaa                      <  2:7055260/93‑1 (MQ=255)

                                                                    ccGGTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAat                     >  2:4801137/1‑93 (MQ=255)

                                                                       gTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATg                  >  1:7158000/1‑93 (MQ=255)

                                                                       gTCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATg                  >  2:3887773/1‑93 (MQ=255)

                                                                        tCGTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGc                 <  2:2422930/93‑1 (MQ=255)

                                                                          gTGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGt               <  1:5457833/93‑1 (MQ=255)

                                                                           tGCCTCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTc              <  2:2008707/93‑1 (MQ=255)

                                                                             cctcctCCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAg            <  2:4843950/93‑1 (MQ=255)

                                                                               tCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCg          >  2:1415358/1‑93 (MQ=255)

                                                                               tCCACCATTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCg          >  1:2836115/1‑93 (MQ=255)

                                                                                ccaccaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCgg         >  2:8458723/1‑93 (MQ=255)

                                                                                 caccaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGa        >  1:1504705/1‑93 (MQ=255)

                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  2:5886350/1‑93 (MQ=255)

                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  2:2327391/1‑93 (MQ=255)

                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  1:2642349/1‑93 (MQ=255)

                                                                                  accaTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGaa       >  1:3846909/1‑93 (MQ=255)

                                                                                    caTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGa     <  2:4984582/93‑1 (MQ=255)

                                                                                     aTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAt    <  1:6167221/93‑1 (MQ=255)

                                                                                     aTTAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAt    >  1:3458740/1‑93 (MQ=255)

                                                                                      ttAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGagc   >  1:8637750/1‑91 (MQ=255)

                                                                                      ttAACAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAtc   >  1:4222531/1‑93 (MQ=255)

                                                                                       taacaaACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAtct  <  1:5739774/93‑1 (MQ=255)

                                                                                       taacaaACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGAtct  <  2:6697957/93‑1 (MQ=255)

CCAGATGATGGATGCGGGTAGCGTCGGTAATGTTGTGCATTATGAACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAGAAGCAGCAAATTATTCATCTGGAATATGCGTCAGCGGAAGATCT  >  NC_000913/3454448‑3454627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`